cÁch lÀm mẤt hoẠt tÍnh aflatoxin

8/6/2019 CCH LM MT HOT TNH AFLATOXIN

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CCH LM MT HOTCCH LM MT HOT

TNH AFLATOXINTNH AFLATOXIN


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GiI THIUGiI THIU

Nhng thng tin trn cc phng tin thng tini chng trong v ngoi nc v tnh hnh

ATVSTP trong thi gian gn y i hichng ta cn phi quan tm nhiu hn na vcht lng n ung

Vit Nam thi gian gn y tnh hnh ngc cc bp n tp th, khu ch xut, khu

cng nghip cng khng km phn phc tp.


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NI DUNG CHNHNI DUNG CHNH

1. CHT C V S NG C THC

PHM

2. AFLATOXIN

3. TC HI C T NM MC

4. LM MT HOT TNH AFLATOXIN


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CHT C V S NG CCHT C V S NG C

THC PHMTHC PHM

Cht c (cn gi l toxin) l nhng

cht m khi vo c th gy ra ri loncc hot ng sinh l, sinh ha bnhthng, biu hin ra bng nhng triuchng bnh tch khc nhau.


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CHT C V S NG CCHT C V S NG C

THC PHM (tt)THC PHM (tt)

Cc trng thi ng c

Ng c cp tnh

Ng c mn tnh

Ng c tim n


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AFLATOXINAFLATOXIN

Vi nmAsperillus flavus vAsperiIlus parasiticus sinh ra,c pht hin vo nm 1960

Trc y ngi ta tm ra Aflatoxin c 6 dn xut quan trng:

B1 v B2: trong nh sng UV ca n fluoress pht ra muxanh nc bin.

G1 v G2: trong nh sng UV ca n fluoress pht ra muxanh l cy.

M1v M2: tm thy trong sa.


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AFLATOXIN (tt)AFLATOXIN (tt)

Aflatoxin l mt hp cht hu c a

mch vng gi l Difurannocumarin


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C ch gy bnh ca Aflatoxin:

lin kt vi ADN trong nhn t bo.S lin kt ny gy c ch enzyme

polymerase ca ARN. N gy tc

dng hn ch trong tng hp ARNv c ch polymerase t-ARN.


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y l nguyn nhn gy gim st tng hp proteintrong t bo. Ngi ta cng chng minh rng vng E

vF-lacton khng bo ha c trong phn t Aflatoxinlm cho hp cht ny c hot tnh gy ung th vcng chnh vng lacton ny gy c ch tng hpAND nhn t bo, do n lm ri lon s tng

trng bnh thng ca t bo (M.F. Nexterin vV.I.A. Vixarinova, 1971).


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S chuyn ha Aflatoxin trongS chuyn ha Aflatoxin trong

c thc th Aflatoxin l tinh th trng, bn vi nhit, khng b

phn hy khi un nu nhit thng thng (

120oC, phi un 30 pht mi mt tc dng), bn vicc men tiu ha, khng bn di nh sng mt triv tia t ngoi

Nhim c cc Aflatoxin gy mt lot cc triuchng cp tnh v mn tnh


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c tnh Aflatoxin trn ng vtc tnh Aflatoxin trn ng vt

th nghim v ngith nghim v ngi Cho n nay, ngi ta tm thi cng nhn kh

nng tc ng ln t bo gan ca Aflatoxin qua

5 giai on:

Tc ng qua li vi AND v c ch ccpolymeraza chu trch nhim tng hp AND

vNgng tng hp AND.


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c tnh Aflatoxin trn ng vt thc tnh Aflatoxin trn ng vt thnghim v ngi(tt)nghim v ngi(tt)

Gim tng hp AND v c ch tng hp

ARN thng tin.

Bin i hnh thi nhn t bo.

Gim tng hp protein


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TC HI C T NM MCTC HI C T NM MC

Gy tn thng t bo gan, thn

Bo mn nim mc ca ng tiu ha

Lm thay i hot ng sinh l

Gim tnh ngon ming ca thc n

Lm h hi cc vitamin trong thc n


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Hm lng Aflatoxin trong mt sHm lng Aflatoxin trong mt snguyn liu lm thc nnguyn liu lm thc n


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LM MT HOT TNHLM MT HOT TNHAFLATOXINAFLATOXIN

Nhit

nh sng

NH3

Phng php sinh hc rATDZ Cht hp th b mt


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LM MT HOT TNH AFLATOXINLM MT HOT TNH AFLATOXINBI NHIT BI NHIT

Nhng nghin cu gn y CFTRI (Central FoodTechnologycal Reseach Institute) Mysor, n , khi

em go nhim Aflatoxin nu di p sut hi ncc n gn 70% Aflatoxin b ph hy. Nu p sut1 at c th ph hy n 50% Aflatoxin. Ngy nayngi ta s dng rng ri phng php ny gimthiu Aflatoxin (Theo thng bo FAO, Rome,1979)


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LM MT HOT TNH AFLATOXINLM MT HOT TNH AFLATOXINBI NHIT (tt)BI NHIT (tt)

Nng kh v chin du c n 65%

Aflatoxin b ph hy. Nu ta emnguyn liu nu bnh thng thAflatoxin khng b h.


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LM MT HOT TNH AFLATOXINLM MT HOT TNH AFLATOXINBI NH SNGBI NH SNG

n ngi ta cn nghin cu cho

thy nu s dng nh sng mt tri vicng 50.000 lux s ph hy c thhon ton cu trc Aflatoxin. Phng

php ny gi l photodegradation of theAflatoxin


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LM MT HOT TNH AFLATOXINLM MT HOT TNH AFLATOXINBI NHBI NH33

c p dng M vo nm 1969 nh l mtphng php x l bnh du bng vi

T nm 1996 Dollear v Gardner ngh s dngkh NH3 di p sut 1,5 3 bars kh c bnhdu phng v bnh du ht bng

Phng php x l ny lm gim 95% hm lngAflatoxin c th xc nh bng phng php ha hc


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nh hng ca x l NH3 di p sut caonh hng ca x l NH3 di p sut caotrn s kh c Aflatoxin ca bnh du phngtrn s kh c Aflatoxin ca bnh du phng


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Lmmt hiu lc Aflatoxin bngLmmt hiu lc Aflatoxin bngphng php sinh hc rATDZphng php sinh hc rATDZ

rADTZ (Recombinant Aflatoxin

Detoxifizym Enzym): Enzym ti t hpkh c tnh Aflatoxin


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Qu trnh nghin cu rADTZQu trnh nghin cu rADTZ

ch to rADTZ phi lm nhiu cng

on: nui cy vi nm, tinh ch protein,to dng gen, ti t hp gen v ln men.


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Bn cht ho hc v c ch khBn cht ho hc v c ch kh

c ca rADTZc ca rADTZ ADTZ l mt men ni t bo c kh nng kh

c ca c t Aflatoxin B1

iu ch t mt loi vi nm v hi cho ngiv ng vt

Khi lng phn t l 51,8 kDa

im ng in pH = 5,4 v kh nng ti u kh c tnh Aflatoxin l pH = 6,8 vnhit l 350C


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Bn cht ho hc v c ch khBn cht ho hc v c ch khc ca rADTZ (tt)c ca rADTZ (tt)

Trong nhng iu kin thch hp, rADTZ

phn ng vi Aflatoxin ph v cutrc vng bifuran lm mt c tnh v cchc nng nh l mt cht i khng

(antidote) ca Aflatoxin


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Qui trnh sn xutQui trnh sn xut rADTZrADTZ

Trc ht l phn lp v sau l tinh chADTZ t vi nm, nhng on mi chuyn bitca gen ADTZ thu c bng cch tinh ch vnhn bn

on gen m ho cho ADTZ c to dng tnhim sc th ca loi nmArmillariellatablescen.


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Qui trnh sn xutQui trnh sn xut rADTZ (tt)rADTZ (tt)

Sau , tng hp protein ti t hp v

tinh ch thng qua hng lot cc h thngtng hp s dng cng ngh gen. ADTZc hot tnh sinh hc lm ph v cu trcca Aflatoxin, do kh c tc ng

gy ung th ca Aflatoxin.


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Tim nng ng dng ca ADTZTim nng ng dng ca ADTZ

Trong cng nghip sn xut thc n gia

sc: rADTZ c th s dng nh mt phgia thm vo loi b Aflatoxin trongthc n gia sc v nhng sn phm tng

t khc nh cht to mi, du u phng


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Tim nng ng dng ca ADTZTim nng ng dng ca ADTZ(tt)(tt)

Trong cng nghip sn xut thc phm:

x l bng rADTZ trong nhng iu kinc bit khng lm nh hng n chtlng v iu kin bo qun ca snphm.


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Tim nng ng dng ca ADTZTim nng ng dng ca ADTZ

(tt)(tt) Trong cng nghip dc phm: rADTZ

cng cho thy nhng du hiu dng nga v tr ung th d dy, ung th gannguyn pht v ung th phi


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Tim nng ng dng ca ADTZTim nng ng dng ca ADTZ(tt)(tt)

Trong th nghim y hc: rADTZ dng

lm thuc th pht hin Aflatoxin v snxut ging chuyn i gen


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NHC iMNHC iM

Ch c th p dng vi nhng l hng b

nhim nng thp. Sn phm sau khix l ch c dng nh mt sn phmph.


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Lmmt hiu lc Aflatoxin biLmmt hiu lc Aflatoxin bicht hp th bmtcht hp th bmt

S dng cc cht hp ph kt dnh

c t loi thi ra ngoi theo phn,lm gim thiu tnh c hi cachng i vi c th


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KT LUNKT LUN

Aflatoxin l mt trong s nhng c t cc tnh cao gy hi cho ngi v vt nui

Hiu c c tnh cng nh c ch gyc ca n s gip cho con ngi cnhng bin php phng nga v loi b

c tnh mt cch trit gp phn hnch tc hi ca n ln sc khe ngi tiudng


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TI LIU THAM KHOTI LIU THAM KHO

PGS.TS LNG C PHM, VI SINH VT HC VV SINH AN TON THC PHM, NXB Nng nghip HNi 2002

PGS.TS DNG THANH LIM, C CHT HCTHC PHM V V SINH AN TON THC PHM, HNNG LM 2008

BO suckhoevadoisong.com.vn CHU VNH TH dch t bi vit trn trang web

http://www.chinamedicinecorp Phng vin tp ch bo chn nui bin son theo t liuca Dr Dwendolyn Jones Biomin, Australia.


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THANKS FOR YOURTHANKS FOR YOURATTENTION!!!ATTENTION!!!

NHM THC HIN:

Nguyn Th Thanh Thuyn

Ng Th Hoi N

Phan Th Thy LinhLp DH06VT

cÁch lÀm mẤt hoẠt tÍnh aflatoxin

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