James Alexander McRoberts

CURRICULUM VITAE

PERSONAL HISTORY:

Business Address: UCLA, Warren Hall 14-103 (MC: 178622)900 Weyburn PlaceLos Angeles, CA 90095

Business Telephone: (310) 267-4834 (UCLA office)Mobile Telephone: (323) 447-9850

E-mail address jim.mcroberts@ucla.eduJMcRoberts@mednet.ucla.edu

EDUCATION:

B.A., Cellular and Organismal Biology, University of California, Santa Barbara, 1973.

Ph.D., Biochemistry, University of California, San Francisco, 1979.

PROFESSIONAL EXPERIENCE:

PRESENT:

Researcher - Recalled, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine; Supervisory Research Chemist, Veteran’s Affairs Greater Los Angeles Healthcare System, 2011-present.

PREVIOUS:

Research Assistant, Department of Biology, University of California, Santa Barbara, 1972-1973.

Teaching and Research Assistant, Department of Biochemistry and Biophysics, University of California, San Francisco, 1974-1979.

Postdoctoral Research Biologist, Department of Biology, University of California, San Diego, 1979-1982.

Postdoctoral Research Physiologist, Division of Gastroenterology, Department of Medicine, University of California, San Diego, 1982

Assistant Research Physiologist, Division of Gastroenterology, Department of Medicine, University of California, San Diego, 1983-1987.

Assistant Adjunct Professor, Division of Gastroenterology, Department of Medicine, Harbor-UCLA Medical Center, Torrance, 1987-1992

Associate Adjunct Professor, Division of Gastroenterology, Department of Medicine, Harbor-UCLA Medical Center, Torrance, 1992-1995

Associate Adjunct Professor, UCLA; Research Physiologist, Veteran’s Administration; CURE: Digestive Disease Research Center, West Los Angeles V.A. Medical Center; 1995-2001

Associate Biochemist, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine; 2001-2002

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Researcher I, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine; 2002-2005

Researcher II, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine; 2005-2008.

Researcher III, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine; 2008-2011.

PROFESSIONAL ACTIVITIES:

Member: Society for Neuroscience, International Association for the Study of Pain.

Ad-hoc reviewer: Neuroscience (7X since last review), Gastroenterology (5X), Merican Jpurnal of Gastroenterology (5X), Brain Research (3X), American Journal of Physiology, Gastrointestinal and Liver Physioliology (1X), Gut (1X), Pain (1X), Journal of Neurogastroenterology and Motility (1X), Journal o f General Medicine (1X), NeuroReport (1X), Journal of Neurophysology (1X).

NIH Study sections: Ad hoc study section member, Special Empahais Panel: Integrative, Functional and Cognitive Neuroscience-K (07/2007) and IFCN-E (5/2008, 10/2009); Neurological Sciences and Disorders (NSD)-C (10/2007) and ZRRI RI-7 (11/09). Chartered member, NDC-C study section (2008 – 2012).

Member; Rome IV Commitee on Age, Gender, Woman’s Health and the Patient, Rome Foundation International (2012-present).

HONORS AND SPECIAL AWARDS:

Faculty scholarship, University of California, Santa Barbara, 1972.Graduation with highest honors, University of California, Santa Barbara, 1973.Regents’ fellowship, University of California, 1974-1975.HHMI pre-doctoral fellowship, 1976Director: Morphology / Cell Biology Core of the Inflammatory Bowel Disease Center, Harbor-UCLA Medical Center, July 1988- July 1992.

RESEARCH GRANTS RECEIVED:

National Institute of Arthritis, Diabetes, Digestive and Kidney Disease"Na+,K+,Cl- cotransport in a kidney epithelial cell line" RO1 AM31619$193,782, 1/1/83 to 12/31/86P.I.: J.A. McRoberts

Academic Senate, University of California, San Diego"Regulatory mechanisms involved in transepithelial transport"$2500, 7/1/84 to 6/30/85P.I.: J.A. McRoberts

Cystic Fibrosis Foundation"Molecular characterization of epithelial Cl- channels"$31,604, 5/1/86 to 4/30/87P.I.: J.A. McRoberts

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Academic Senate, University of California, San Diego"Na+,K+,Cl- cotransport and [3H]piretanide receptors"$2500, 7/1/86 to 7/1/87P.I.: J.A. McRoberts

Cystic Fibrosis Foundation"Molecular characterization of epithelial Cl- channels"$31,860 6/1/87 to 4/30/88P.I.: J.A. McRoberts

Research and Education Institute, Initial intramural support;"Research laboratory support."$6,000; 2/1/88-6/30/88P.I.: J.A. McRoberts

Research and Education Institute, Institutional Research Project;"Intracellular messengers involved in Cl- secretion."$3,000, 3/1/88-2/28/89P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases: Inflammatory Bowel Disease Center Pilot Project; W.J. Snape, Jr., Director. "Intracellular second messengers involved in chloride ion secretion."$15,000, 1/1/88-7/31/88P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases: Inflammatory Bowel Disease Center Pilot Project; W.J. Snape, Jr., Director. "Expression of electrolyte transport by primary rabbit colonocytes in culture."$15,000, 8/1/88-8/31/88Co-P.I.s: J.A. McRoberts & A.Vidrich

Crohn's and Colitis Foundation of America "Regulation of Intestinal Permeability by Insulin and Insulin-like Growth Factors"; $144,424, 10/1/89-9/31/91P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases: Inflammatory Bowel Disease Center Pilot Project; W.J. Snape, Jr., Director. "Growth and differentiation of normal epithelial cells in culture." $7,925, 11/1/90-10/31/91 P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases:"Regulation of epithelial permeability by growth factors" RO1 DK42874$318,099, 8/1/91 to 7/31/94P.I.: J.A. McRoberts

Harbor-UCLA Research & Education Institute, Institutional Research Project."Discovery of messenger RNAs associated with changes in epithelial permeability."

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$4,000, 7/1/93-6/31/94P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases: CURE:VA/UCLA Gastroenteric Biology Center, Pilot project, J. H. Walsh, Director“Discovery of genes regulating epithelial permeability”$12,000, 1/1/95-12/31/96 P.I.: J.A. McRoberts

Pharmacia & Upjohn, Inc.“Endoscopic, morphologic, and physiologic basis of Irinotcan (CPT-11)-induced diarrhea”$175,000, 1/1/97-12/31/97 P.I. R. Hecht; Co-P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases: “Peripheral NMDA receptors in visceral nociception” RO1 DK58173$1,150,000, 5/1/01-4/30/06 P.I.: E.A. Mayer; Co-P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases “Chronic Stress and Visceral Nociception” R21 DK066065$200,000, 2/1/04-1/31/06P.I.: J.A. McRoberts

GlaxoSmithKline“Regulation of CGRP Release by 5-HT3 Receptors” GSK 004888$72,360; 02/01/06 – 01/31/07 P.I.: J.A. McRoberts

Center for Neurovisceral Sciences & Women’s Health: Pilot and feasibility study National Institute of Diabetes, Digestive and Kidney Diseases P50 DK64539“Sex dependent expression and regulation of NMDA receptors in DRG neurons” $15,000; 08/01/06 – 07/31/07P.I.: J.A. McRoberts

National Institute of Diabetes, Digestive and Kidney Diseases “Peripheral NMDA receptors in visceral nociception” RO1 DK58173$760,000; 9/1/06 – 8/31/10P.I.: J.A. McRoberts

CURE Pilot and feasibility study, NIDDK, P30 DK041301 “Role of protein kinase D in nociception”$25,000; 3/1/09 – 10/31/10P.I.: J.A. McRoberts

Department of Veteran Affairs (Rehabilitation Research & Development Service) IRX000378A“Neurotransmitter control of substance P release in the spinal cord” Role: Co-Investigator 3/01/2011 – 9/30/14

NIH/NIDA R01DA033059

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“NMDA receptors in primary afferents”$ 277,000 / yr; 07/01/2012 – 04/30/2017MPI: Marvizon & McRoberts

NIH/NIDDK, 2U01DK082370-06 “MAPP Research Network Second Phase”$900,000; 09/15/2014 – 06/30/2019Role: Co-Investigator

TEACHING EXPERIENCEC. Tony Tran, undergraduate, UC San Diego, Bio 199 (faculty sponsor: Milton Saier). 1982-

1983.

Christine Cartwright, M.D., Resident, UC San Diego School of Medicine, Research Rotation, 1985.

Hoon Kang, high school graduate, Summer Student/Teacher Research Program, Harbor-UCLA Medical Center, summer 1988.

Richard Aranda, M.D., Research rotation during Residency Program at Harbor-UCLA Medical Center, 1988.

BMD260, Disease Process GI, UCR-UCLA combined program, Harbor-UCLA Medical Center; Dr. McRoberts contributed two lectures during the three week course. 1989-1995.

Helen Park, high school graduate, Summer Student/Teacher Research Program, Harbor-UCLA Medical Center, summer 1989.

Lin Chang, M.D., Research rotation during Residency Program at Harbor-UCLA Medical Center, 1989.

Mario Sottili, M.D., Visiting Fellow, Harbor-UCLA Medical Center, 1991-1992.

Sachiko Morrey, high school science teacher, Summer Student/Teacher Research Program, Harbor-UCLA Medical Center, summer 1992.

Maria Cabero, high school graduate, Summer Student/Teacher Research Program, Harbor-UCLA Medical Center, summer 1993.

Brent Kovacs, M.D., Research rotation during Residency Program at Harbor-UCLA Medical Center, 1993.

Susan Saucedo, high school graduate, Summer Student/Teacher Research Program, Harbor-UCLA Medical Center, summer 1994. Sammy Y. Shon, UCLA undergraduate student, supervisor during 3 quarters of Special Studies 199 (Physiology faculty sponsor: Earnest Wright). 1992-1994.

Jörg Zeeh, M.D., Postdoctoral Fellow, Division of Gastroenterology, Harbor-UCLA Medical Center, 1993-1995.

Timothy Fitzsimmons, graduate student in Biological Sciences, UC San Diego, Ph.D. Nov. 1997, under the direction of Steve Pandol, MD. Provided extensive supervision of research projects. 1995-1997.

Victor V. Chaban, Ph.D., Postdoctoral Fellow, Neuroenteric Disease Program, UCLA School of Medicine, 1999-2001.

Jingjiang Nie, M.D., Ph.D., Postdoctoral Fellow, Neuroenteric Disease Program, UCLA School of Medicine, 2001-2003.

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Ines Schwetz, M.D., Postdoctoral Fellow, Neuroenteric Disease Program, UCLA School of Medicine, 2001-2003

Jichang Li, Ph.D. Postdoctoral Fellow, CNS/WH, UCLA School of Medicine, 2002-2007

Sylvie Bradesi, Ph.D. Postdoctoral Fellow, CNS/WH, UCLA School of Medicine, 2002-2006

Erin, Vickie, undergraduate volunteers, summer 2003

Muriel Larauche, Ph.D., Postdoctoral Fellow, Center for Neurovisceral Sciences & Women’s Health, UCLA School of Medicine, 2004-2005.

Craig Berzofsky, Pre-med student/technician, Jan-July 2004.

Yash Mittal, UCLA undergraduate student, July 2004-Dec 2007.

Nanyi Zhang, UCLA undergraduate student, Sept 2006-Jun 2009.

Murphy Bergstrom, UCLA undergraduate student, Dec 2007-Jun 2009

Brian Nguyen, UCLA undergraduate student, March 2007-Jun 2010

Samantha Jagannathan, UCLA undergraduate student, Sep 2008-Jun 2010

Shane Shahrestani, UCLA undergraduate student, Sep 2013-Jun 2014

INVITED LECTURES AND PRESENTATIONS

"Mutants of the MDCK cell line defective in Na+, K+ and Cl- cotransport." West Coast Salt and Water Club, 2-12-83.

"Characterization of electrolyte transport processes in a human colonic epithelial cell line." National Convention of the American Gastroenterological Association, 5-10-83.

"Hormonally regulated transepithelial transport by a human epithelial cell line derived from colon carcinoma.” West Coast Salt and Water Club, 2-14-85.

"Is the Na+,K+,Cl- cotransporter a receptor for loop-diuretics?", Department of Pharmacology, University of Missouri, Columbia, 11-13-86.

"Transcellular Cl- secretion by a cultured epithelial cell line", Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, 9-12-86.

"Is the Na+,K+,Cl- cotransporter a receptor for loop-diuretics ?", Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, 1-18-87.

"Is the Na+,K+,Cl- cotransporter a receptor for loop-diuretics ?", Department of Physiology and Biophysics, USC School of Medicine, Los Angeles, CA, 2-4-87.

"Differentiated Cell Lines as Model systems for the study of epithelial ion transport", IBD Center, Harbor/UCLA Medical Center, Torrance, CA, 7-8-87.

"Pharmacology of antisecretory drugs: structural and functional aspects on transport proteins", Intestinal Secretion of Fluid and Electrolytes: Physiology, Methodology and Pharmacology, sponsored by University of Goteborg and A. B. Hassle, Fiskenbackskil, Sweden, 8-14-87.

"Growth and differentiation of the human colonic epithelial cell line, T84, in hormonally defined, serum-free media", IBD Center, semi-annual meeting, 11-18-87.

"Backwards and forwards through the chloride channel", Center Directors Meeting, Harbor-UCLA Medical Center, Torrance, CA 2-22-88.

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"Regulation of paracellular permeability by insulin and insulin-like growth factors", Division of Pulmonary and Critical Care Medicine, Harbor-UCLA Medical Center, 3-2-89.

"Regulation of paracellular permeability by insulin and insulin-like growth factors", IBD Center monthly meeting 3-15-89.

"Electrolyte transport and regulation", Harbor-UCLA Postdoctoral Basic Science Course, 4-10-89

"Insulin and insulin-like growth factors regulate the paracellular permeability of T84 colonic epithelial cells", National Convention of the American Gastroenterological Association, 5-15-89.

"Insulin regulates the paracellular permeability of T84 colonic epithelial cells", Faculty Society of Harbor-UCLA Medical Center first annual Ph.D. research competition, 9-25-89.

Mucosal permeability in bowel inflammation", IBD Clinical Conference, 1-18-90

"Regulation of intestinal permeability by growth factors", Center for Ulcer Research and Education (CURE), V.A. Wadsworth , 5-2-90.

Modulation of growth factor and cytokine mediated changes in paracellular permeability by media glucose and iron", Third Annual Gastroenteric Biology conference, Chicago, 11-1-90.

"Regulation of paracellular permeability by growth factors", Department of Pharmacology, University of Missouri - Columbia, 11-15-90.

"Regulation of paracellular permeability by growth factors", Alza Inc., Palo Alto, CA, 12-19-90.

Regulation of colonic epithelial cell tight junction permeability by insulin-like growth factors", 2nd International IGF Symposium, San Francisco, 1-15-91.

"Regulation of epithelial permeability by growth factors by growth factors and cytokines", Department of Physiology and Cell Biology, University of Texas, Houston, 3-11-91.

Regulation of epithelial permeability by growth factors by growth factors and cytokines", Department of Pharmacology, University of Southern California, 6-17-91.

"Regulation of epithelial permeability by growth factors by growth factors and cytokines", Telios Inc. and La Jolla Cancer Research Foundation, San Diego, 7-8-91.

"Insulin-like growth factors and effects on intestinal epithelial cells"; Growth Factor Group, Harbor-UCLA Medical Center, 10-4-91.

"E. Coli heat stable enterotoxin mediates chloride secretion through cGMP and protein kinase A"; National Convention of the American Gastroenterological Association, San Francisco, 5-12-92

"Regulation of intestinal epithelial paracellular permeability", UCLA Combined Nephrology Research Series, V.A Wadsworth, 8-28-92.

"Cultured epithelial cells as tools to investigate transepithelial transport and barrier function", Amgen, Inc, Thousand Oaks, 4-27-93.

Insulin-like growth factors, their receptors and binding proteins", L.A. Growth Factor & Cytokine Club (interest group from UCLA, USC, V.A. Wadsworth, V.A. Sepulveda, & Harbor-UCLA Medical Centers), Harbor-UCLA, 10-12-93.

Cultured epithelial cells as tools to investigate transepithelial transport and barrier function", Proctor & Gamble, Cincinnati, OH, 1-9-95

Regulation of gastrointestinal epithelial permeability by insulin and insulin-like growth factors”; Immunex Corporation, Seattle WA, 5-25-95

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Binding and signal transduction properties of human CCK receptor chimeras”, CURE: DDRC Signal Transduction Affinity Group, West L.A. V. A. Medical Center, 4-25-96.

Role of cyclic ADP ribose in pancreatic acinar cells”, CURE: DDRC Signal Transduction Affinity Group, West L.A. V. A. Medical Center, 5-9-96

Ryanodine receptors and Ca2+ release in pancreatic acinar cells”. Zymogenetics, Seattle, Washington, 7-15-99.

“Stress-induced visceral hyperalgesia in the rat maternal separation model: Role of opioid and CRF receptors” Brain-Gut Meeting, Oxford, UK, 7-13-2001.

"Ifenprodil and other subtype selective antagonists of the NMDA receptor" Pain-Neurobiology Seminar series, September 16, 2003.

“NMDA, NMDA receptors and spinal afferent activity” Pain-Neurobiology Seminar series, February 3, 2004. 

"The revolution in mouse genetics: The nuts and bolts of regional and conditional mutagenesis" Pain-Neurobiology Seminar series, April 6, 2004.

“Role of NMDA receptors in dorsal root ganglia neurons in nociception” CNS/WH Translational Seminar series, July 12, 2004

Peripheral NMDA receptors: Gatekeepers of visceral pain” Annual meeting of the Center for Neurovisceral Sciences and Women’s Health, January 21, 2005.

“The Role of NMDA Receptors On Spinal Afferents In Visceral Pain and Hyperalgesia” Digestive Diseases Week, Los Angeles, May 23, 2006.

“Sex dependent expression and regulation of NMDA receptors in DRG neurons” Center for Neurovisceral Sciences and Women’s Health; Annual Research meeting, Feb 1, 2007.

Sensory neuron NMDA receptors in pain transmission” CURE: Digestive Diseases Research Center Annual Meeting, Los Angeles, March 16, 2007.

“Characterization of NMDA receptors on spinal afferents and their role in nociception”, UCSD, Dept. of Anesthesiology seminar series, Jan 9, 2008.

Sex dependent regulation of NMDA receptors in DRG neurons”, CURE seminar series, Feb 13, 2008.

“Role of NMDA receptors spinal afferents and in nociception”, Center for Study of Opioid Receptors and Drugs of Abuse (CSORDA) seminar series, Jun 6, 2009.

PUBLICATIONS

RESEARCH PAPERS (PEER REVIEWED)

1. Graf, L.H., Jr., McRoberts, J.A., Harrison, T.M. and Martin, D.W., Jr.: Increased PRPP synthetase activity in cultured rat hepatoma cells containing mutations in the hypoxanthine-guanine phosphoribosyltransferase gene. J. Cell. Physiol. 88: 331-342, 1976.

2. McRoberts, J.A. and Martin, D.W., Jr.: Submolecular characterization of a human mutant purine-nucleoside phosphorylase. J. Biol. Chem. 255: 5605-5615, 1980.

3. Rindler, M.J., McRoberts, J.A. and Saier, M.H., Jr.: Na+,K+ cotransport in the Madin Darby Canine Kidney (MDCK) cell line: Kinetic characterization of the interaction between Na+ and K+. J. Biol. Chem. 257: 2254-2259, 1982.

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4. McRoberts, J.A., Erlinger, S., Rindler, M.J. and Saier, M.H., Jr.: Furosemide-sensitive salt transport in the Madin Darby Canine Kidney (MDCK) cell line: Evidence for the cotransport of Na+,K+,Cl-. J. Biol. Chem. 257: 2260-2266, 1982.

5. McRoberts, J.A., Tran, C.T. and Saier, M.H., Jr.: Characterization of low-potassium resistant mutants of the Madin Darby Canine Kidney (MDCK) cell line with defects in NaCl/KCl symport. J. Biol. Chem. 258: 12320-12326, 1983.

6. Dharmsathaphorn, K., Yamashiro, D.J., Lindeborg, D., Mandel, K.G., McRoberts, J.A., and Ruffolo, R.R.: Effects of structure-activity relationships of alpha-adrenergic compounds on electrolyte transport in the rabbit ileum and rat colon. Gastroenterology 86: 120-128, 1984.

7. Dharmsathaphorn, K., Mandel, K.G., McRoberts, J.A., Tisdale, L.D. and Masui, H.: A human colonic tumor cell line that maintains vectorial electrolyte transport. Am. J. Physiol. 246: G204-G208, 1984.

8. Dharmsathaphorn, K., Mandel, K.G., Masui, H. and McRoberts, J.A.: Vasoactive intestinal polypeptide-induced chloride secretion by a human colonic epithelial cell line: Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system. J. Clin. Invest. 75: 462-471, 1985.

9. Weymer, A., Huott, P., McRoberts, J.A. and Dharmsathaphorn, K.: Chloride secretory mechanism induced by prostaglandin E1 in a colonic epithelial cell line. J. Clin. Invest. 76: 1828-1836, 1985.

10. Cartwright, C.A., McRoberts, J.A., Mandel, K.G. and Dharmsathaphorn, K.: Synergistic action of cyclic AMP and calcium mediated chloride secretion in a colonic epithelial cell line. J. Clin. Invest. 76: 1837-1842, 1985.

11. McRoberts, J.A., Beuerlein, G. and Dharmsathaphorn, K.: Cyclic AMP and Ca2+ activated K+ transport in a human colonic epithelial cell line. J. Biol. Chem. 260: 14163-14172, 1985.

12. Mandel, K.G., Dharmsathaphorn, K. and McRoberts, J.A.: Characterization of a cyclic AMP-activated Cl- transport pathway in the apical membrane of a human colonic epithelial cell line. J. Biol. Chem. 261: 704-712, 1986.

13. Mandel, K.G., McRoberts, J.A., Beuerlein, G., Foster, E.S. and Dharmsathaphorn, K.: Ba++ inhibition of VIP and A23187 stimulated Cl- secretion by T84 cell monolayers. Am. J. Physiol. 250: C486-C494, 1986.

14. Dharmsathaphorn, K., Huott, P., Cartwright, C.A., McRoberts, J.A., Mandel, K.G., and Beuerlein, G.: Inhibition of ATP levels by quinidine in a human colonic epithelial cell line. Am. J. Physiol. 250: G806-G813, 1986.

15. Giesen-Crouse, E. and McRoberts, J.A.: Coordinate expression of piretanide receptors and Na+,K+,Cl- cotransport activity in MDCK cell mutants. J. Biol. Chem. 262:17393-17397, 1987.

16. Huott, P.A., Liu, W., McRoberts, J.A., Giannella, R.A. and Dharmsathaphorn, K.: The mechanism of action of E. coli heat stable enterotoxin in a human colonic cell line. J. Clin. Invest. 82:514-523, 1988.

17. McRoberts, J.A., Aranda, R., Riley, N., Kang, H.: Insulin regulates the paracellular permeability of cultured intestinal epithelial cell monolayers. J. Clin. Invest. 85:1127-1134, 1990.

18. Lira, M., Schteingart, C.D., Steinbach, J.H., Lambert, K., McRoberts, J.A., Hofmann, A.F.: Sugar absorption by the biliary ductular epithelium of the rat: Evidence for two transport systems. Gastroenterology 102:563-571, 1992.

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19. McRoberts, J.A., Riley, N.: Regulation of T84 colonic epithelial cell monolayer permeability by insulin-like growth factors. Am. J. Physiol. 262:C207-C213, 1992.

20. Hyman, P.E., Kimura, S., Tomomasa, T., Snape, W.J., Jr., McRoberts, J.A.: Neonatal changes in the substance P receptor on rabbit gastric smooth muscle Am. J. Physiol. 262:G291-G297, 1992.

21. Ennes, H.S., McRoberts, J.A., Hyman, P.E., Snape, W.J., Jr.: Characterization of colonic circular smooth muscle cells in culture. Am. J. Physiol. 263:G365-G370, 1992.

22. Yuan, Q.X., Lakshmanan, J. McRoberts, J.A., Yagi, H., Hyman, P.E.: Newborn rabbit gastric smooth muscle cell culture: EGF and TGF, but not IGF-1 are potent mitogens. J. Ped. Gastro. Nutr.17:153-160, 1993.

23. LeDuc, L.E., J.A. McRoberts, A. Vidrich: Eicosanoid production by a differentiated canine colonic epithelial cell line, VNCC. Gastroenterology, 106:297-305, 1994.

24. Procaccino, F., M. Reinshagen, P. Hoffmann, J.M. Zeeh, J. Laksmanan, J.A. McRoberts, A. Patel, S. French and V.E. Eysselein. Epidermal growth factor reduces mucosal injury in an experimental model of colitis in rats. Gastroenterology, 107:12-17, 1994.

25. McRoberts, J.A., Riley, N.E.: Role of insulin and insulin-like growth factor receptors in the regulation of T84 cell monolayer permeability. Am. J. Physiol., 267:G883-G891, 1994.

26. McRoberts, J.A., Riley, N.E.: Modulation of growth factor and cytokine-induced increases in T84 cell monolayer permeability by media components. Am. J. Physiol., 267:C537-C543, 1994.

27. Zeeh, J., P. Hoffmann, M. Sottili, H.S. Ennes, V.E. Eysselein, J.A. McRoberts. Up-regulation of insulin-like growth factor-I binding sites in experimental colitis in rats. Gastroenterology, 108:644-652, 1995.

28. Zeeh, J.M., F. Procaccino, P. Hoffmann, S.L. Aukerman, F. Procaccino, J.A. McRoberts, S. Soltani, G.F. Pierce, J. Lakshmanan, D. Lacey, V.E. Eysselein. Kerotinocyte growth factor ameliorates mucosal injury in an experimental model of colitis in rats. Gastroenterology, 110:1077-1083, 1996.

29. Zeeh, J.M., H.S. Ennes, P. Hoffmann, F. Procaccino, V.E. Eysselein, W.J. Snape, Jr., and J.A. McRoberts. Expression of insulin-like growth factor I receptors and binding proteins by colonic smooth muscle cells. Am.J.Physiol. 272:G481-G487, 1997.

30. Hoffmann, P., J.M. Zeeh, J. Lakshmanan, V.S. Wu, F. Procaccino, M. Reinshagen, J.A. McRoberts, and V.E. Eysselein. Increased expression of transforming growth factor alpha precursors in acute experimental colitis in rats. Gut 41:195-202, 1997.

31. Wu, V., M. Yang, J.A. McRoberts, J. Ren, R. Seensalu, N. Zeng, M. Dagrag, M. Birnbaumer, and J.H. Walsh. First intracellular loop of the human cholecystokinin-A receptor is essential for cyclic AMP signaling in transfected HEK-293 cells. J.Biol.Chem. 272:9037-9042, 1997.

32. Fitzsimmons, T.J., J.A. McRoberts, K.H. Tachiki, and S.J. Pandol. Acyl-coenzyme A causes Ca2+ release in pancreatic acinar cells. J.Biol.Chem. 272:31435-31440, 1997.

33. Zeeh, J.H., N. Mohapatra, P.K. Lund, V.E. Eysselein, and J.A. McRoberts. Differential expression and localization of IGF-I and IGF binding proteins in inflamed rat colon. J. Receptor Signal Transduction Res., 18:265-280, 1998.

34. Kölbel, C.B., G. Holtmann, J.A. McRoberts , S. Schöler, P. Aengenvoordt, M.V. Singer and E.A. Mayer. Involvement of chloride channels in the receptor-mediated activation of longitudinal colonic smooth muscle. Neurogastro. Motility, 10: 489-498, 1998.

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35. Young, S.H., H.S. Ennes, J.A. McRoberts, V.V. Chaban, S.K. Dea, and E.A. Mayer. Calcium waves in colonic myocytes produced by mechanical and receptor-mediated stimulation. Am.J.Physiol, 276: G1204-G1212, 1999.

36. Gschossmann, J.M., V.V. Chaban, J.A. McRoberts, H.E. Raybould, S.H. Young, H.S. Ennes, T. Lembo, E.A. Mayer. Mechanical activation of dorsal root ganglion cells in vitro: Comparison with capsaicin and modulation by kappa opioids. Brain Research, 856: 101-110, 2000.

37. Fitzsimmons, T.J., I. Gukovsky, J.A. McRoberts, E. Rodriguez, F.A. Lai, and S.J. Pandol. Multiple isoforms of the ryanodine receptor are present in rat pancreatic acinar cells. Biochem. J. 351: 265-271, 2000.

38. Abreu M.T., A.A. Palladino, E.T. Arnold, R.S. Kwon, J.A. McRoberts. Modulation of Barrier Function During Fas-Mediated Apoptosis in Human Intestinal Epithelial Cells. Gastroenterology 2000 119: 1524-1536, 2000.

39. Chaban, V.V., J.A. McRoberts, H.S. Ennes, E.A. Mayer. Nitric oxide synthase inhibitors enhance mechanosensitive Ca2+ influx in cultured DRG neurons. Brain Research 903:74-85, 2001.

40. McRoberts, J.A., S. Coutinho, J.C.G. Marvizón, E.F. Grady, M. Tognetto, J.N. Sengupta, H.S. Ennes, V.V. Chaban, S. Amadesi, C. Creminon, T. Lanthorn, P. Geppetti, N.W. Bunnett, and E.A. Mayer. Role of peripheral N-methyl-D-aspartate (NMDA) receptors in visceral nociception in rats. Gastroenterology 120:1737-1748, 2001.

41. Marvizon, J.C., J.A. McRoberts, H.S. Ennes, T. Johansson, B. Corneliussen, L. Jinton, E.A. Mayer. Subunit composition of NMDA receptors in rat dorsal root ganglia. J. Comp. Neuro. 2002;446:325-341.

42. Coutinho, S.V., P.M. Plotsky, M. Sablad, J.C. Miller, H. Zhuo, A.I Bayati, J.A. McRoberts, E.A. Mayer. Neonatal maternal separation alters stress-induced responses to viscerosomatic nociceptive stimuli in the adult rat. Amer J Physiol Gastrointest Liver Physiol 2002;282:G307-G316.

43. Million M, Grigoriadis DE, Sullivan S, Crowe PD, McRoberts JA, Zhou H, Saunders P, Maillot C, Mayer EA, Tache Y. A novel water soluble selective CRF1 receptor antagonist, NBI 35965, blunts stress-induced visceral hyperalgesia and colonic motor function in rats. Brain Research. 985:32-42, 2003.

44. Schwetz I, Bradesi S, McRoberts JA, Sablad M, Miller JC, Zhou H, Ohning G, Mayer EA. Delayed Stress-Induced Visceral Hyperalgesia in Male Wistar Rats: Role of NK1 and CRF1 receptors. Am. J. Physiol., 286:G683-91, 2004.

45. Amadesi S, Nie J, Vergnolle N, Cottrell GS, Grady EF, Trevisani M, Manni C, Geppetti P, McRoberts JA, Ennes H, Davis JB, Mayer EA, Bunnett NW Protease-activated receptor 2 sensitizes the capsaicin receptor transient receptor potential vanilloid receptor 1 to induce hyperalgesia. J Neurosci. 24:4300-12, 2004.

46. Li J, McRoberts JA, Nie J, Ennes HS, Mayer EA. Electrophysiological characterization of N-methyl-D-aspartate receptors in rat dorsal root ganglia neurons. Pain, 109:443-52, 2004.

47. Chaban V, Li J, Mayer EA, Ennes H, McRoberts JA. NMDA receptor modulation of mechanically induced calcium transients in rat DRG neurons. Neuroscience 128: 347–357, 2004.

48. Bradesi S, Schwetz I, Ennes HS, Lami C, Ohning G, Fanselow M, McRoberts JA, Mayer EA. Repeated exposure to water avoidance stress in rats: a new model for sustained visceral hyperalgesia. Am J Physiol Gastrointest Liver Physiol 289:G42-53, 2005.

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49. Schwetz I, McRoberts JA, Coutinho SV, Bradesi S, Gale G, Fanselow M, Mulugeta M, Ohning G, Plotsky PM, Tache Y and Mayer EA. Corticotropin releasing factor receptor 1 mediates acute and sustained stress-induced visceral hyperalgesia in maternally separated Long Evans rats. Am J Physiol Gastrointest Liver Physiol. 289:G704-12, 2005.

50. J Li, HS Ennes, C Berzofsky, EA Mayer, JA McRoberts. Experimental colitis causes up-regulation of NMDA receptors on dorsal root ganglia neurons. Am J Physiol Gastrointest Liver Physiol. 291:G219-28, 2006.

51. Million M, Wang L, Wang Y, Adelson DW, Yuan PQ, Maillot C, Coutinho SV, McRoberts JA, Bayati A, Mattsson H, Wu VS, Wei JY, Rivier J, Vale W, Mayer EA, Tache Y. CRF2 receptor activation prevents colorectal distension-induced visceral pain and spinal ERK1/2 phosphorylation in rats. Gut. 55:172-81, 2006.

52. Bradesi S, Kokkotou E, Simeonidis S, Patierno S, Ennes HS, Mittal Y, McRoberts JA, Ohning G, McLean P, Marvizon JC, Sternini C, Pothoulakis C, Mayer EA. The role of neurokinin 1 receptors in the maintenance of visceral hyperalgesia induced by repeated stress in rats. Gastroenterology 130:1729-42, 2006.

53. McRoberts JA, Li J, Ennes HS, Mayer EA. Sex-dependent differences in the activity and modulation of NMDA receptors in rat DRG neurons. Neuroscience 148:1015–1020, 2007.

54. Larauche MH, Bradesi S, Million M, McLean P, Tache Y, Mayer EA, McRoberts JA. Corticotropin releasing factor type 1 receptors mediate the sustained visceral hyperalgesia induced by a repeated psychological stress in rats. Am J Physiol Gastrointest Liver Physiol., 294:1033-1040, 2008.

55. Bryant CD, Zhang NN, Sokoloff G, Fanselow MS, Ennes HS, Palmer AA, and McRoberts JA. Behavioral differences among C57BL/6 substrains: Implications for transgenic and knockout studies. J. Neurogenet., 22:315-31, 2008.

56. McRoberts, JA, HS Ennes, J.C Marvizon, EA Mayer, M. Fanselow, B Vissel. Selective knockdown of NMDA receptors in primary afferent neurons decreases pain during phase 2 of the formalin test. Neuroscience, 172:474-82, 2011. PMID: 20974228 .

57. Okano-Matsumoto S, McRoberts JA, Taché Y, Adelson DW. Differential afferent and efferent responses to intravenous CCK-8 in subdivisions of the rat ventral gastric vagus indicate distinct vago-vagal reflex arcs controlling distal vs proximal stomach function. J Physiol. 589 :371-93, 2011. PMID: 21078593

58. Kilpatrick LA, JS Labus, K Coveleskie, C Hammer, JM Jarcho, JA McRoberts, G Rappold, JA Bueller, B Suyenobu, JC Papp, S Dandekar, B Niesler & EA Mayer. The 5HT3A gene polymorphism c. -42C>T is associated with amygdala responsiveness in patients with irritable bowel syndrome. Gastroenterology 140: 1943-51, 2011. PMCID: PMC3757951

59. Bradesi S, Golovatscka V, Ennes HS, McRoberts JA, Karagiannides I, Bakirtzi K, Pothoulakis C, Mayer EA. Role of astrocytes and altered regulation of spinal glutamatergic neurotransmission in stress-induced visceral hyperalgesia in rats. Am J Physiol Gastrointest Liver Physiol. 301:G580-9, 2011. PMC3174538.

60. Chen W, McRoberts JA, Marvizón JC. μ-Opioid receptor inhibition of substance P release from primary afferents disappears in neuropathic pain but not inflammatory pain. Neurosci. 267:67-82, 2014; PMC3998911

61. Chen W, Walwyn W, Ennes HS, Kim H, McRoberts JA, Marvizón JC. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. Eur J Neurosci. 39:1439-54, 2014. PMC4122572.

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62. Chen W, McRoberts JA, Marvizón JC. μ-Opioid receptor inhibition of substance P release from primary afferents disappears in neuropathic pain but not inflammatory pain. Neuroscience 267:67-82, 2014. PMC3998911

63. Walwyn WM, Chen W, Kim H, Minasyan A, Ennes HS, McRoberts JA, Marvizón JC. Sustained Suppression of Hyperalgesia during Latent Sensitization by μ-, δ-, and κ-opioid receptors and α2A Adrenergic Receptors: Role of Constitutive Activity. J Neurosci. 2016 36:204-21. PMC4701961

BOOK CHAPTERS

1. Gudas, L.J., Zannis, V.I., McRoberts, J.A., Martin, D.W., Jr.: Characterization of mutant subunits of human purine nucleoside phosphorylase. In Inborn Errors of Specific Immunity. B. Pollara, R.J. Pickering, M.J. Meuwissen, I.H. Porter, eds. Academic Press, 1979, pp 173-185.

2. McRoberts, J.A., Taub, M., Saier, M.H., Jr.: The Madin Darby Canine Kidney (MDCK) cell line. In Functionally Differentiated Cell Lines. G. Sato, ed. Alan R. Liss, New York, 1981, pp 117-139.

3. Dharmsathaphorn, K., Mandel, K.G., McRoberts, J.A., and Masui, H.: Utilization of a human colonic tumor cell line as a model to study electrolyte transport in the intestine. In Intestinal Absorption and Secretion. K. Elve, K. Heintze, W. Rummel, E. Skadhague, K.H. Soergel, eds. MPT Press, Ltd., Lancaster, 1984, pp 325-333.

4. Cartwright, C.A., McRoberts, J.A., Mandel, K.G., Masui, H. and Dharmsathaphorn, K.: Electrolyte transport in a human colonic cell line - regulation by cAMP and calcium. In Regulation of Intestinal Electrolyte Transport. M. Donowitz and G.W. Sharp, eds. Alan R. Liss, inc., New York, 1984, pp 65-75.

5. McRoberts, J. A. and Barrett, K. E.: Hormone regulated ion transport in T84 human colonic cells; In Functional Epithelial Cells in Culture; K. S. Matlin and J. D. Valentich, editors. Alan R. Liss, Inc., New York, 1989, pp235-265.

6. Kaunitz, J.D., K.E. Barrett, J.A. McRoberts,. Chapter 14: Secretion and Absorption: Small Intestine and Colon. In Textbook of Gastroenterology. T. Yamada, D. Alpers, D. Powell, F. Silverstein, C. Owyang, editors. 2nd edition., 1995, pp 326-360.

CHAPTERS (IN PREPARATION)

7. Heitkemper M, Houghton L, Halpert A., Crowell M, Toner B, McRoberts J, Emanuel A, Chang L. Chapter 6: Age, Gender, Woman’s Health and the Patient; in Rome IV: The Functional Gastrointestinal Disorders.

REVIEWS

1. Saier, M.H., Jr., Boerner, P., Grenier, F.C., McRoberts, J.A., Rindler, M.J., Taub, M. and U, H.S.: Sodium entry pathways in renal epithelial cell lines. Mineral Electrolyte Metab. 12: 42-50, 1986.

2. Bradesi S, McRoberts J, Anton P, Mayer EA. Inflammatory bowel disease and irritable bowel syndrome: Separate or unified? Current Opinion in Gastroenterology, 19: 336-342, 2003.

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ABSTRACTS

1. Martin, D.W., J.A. McRoberts, L.J. Gudas. Molecular characterization of a human mutant purine nucleoside phosphorylase. J Clin Chem Clin Bio 17: 425, 1979

2. Rindler, M.J., McRoberts, J.A., Saier, M.H., Jr.: Na+-K+ cotransport in dog kidney epithelial cells (MDCK). J. Supramolec. Struct., Suppl 4: 86, 1980.

3. McRoberts, J.A., Rindler, M.J., Erlinger, S., Saier, M.H., Jr.: Na+,K+,Cl- cotransport in the MDCK cell line. J. Cell. Biol. 91: 261a, 1981.

4. Dharmsathaphorn, K., Yamashiro, D.J., Lindeborg, D., Mandel, K., McRoberts, J.A. and Ruffolo, R.R.: Structure-activity relationships of 2 adrenergic compounds in the intestine: The possibility that 2 adrenergic receptors in the gut and brain are different. Gastroenterology 84: 1247, 1983.

5. McRoberts, J., Mandel, K., Masui, H., and Dharmsathaphorn, K.: Characterization of electrolyte transport processes in a human colonic tumor line. Gastroenterology 84: 1247, 1983.

6. Dharmsathaphorn, K. Mandel, K., McRoberts, J. Tisdale, L.D., and Masui, H.: Alteration of electrolyte transport in a human colonic epithelial cell line in response to peptide hormones and neurotransmitters. Gastroenterology 84: 1136, 1983.

7. Cartwright, C., McRoberts, J., Masui, H., Lindeborg, D. and Dharmsathaphorn,K.: Quinidine reversal of VIP-stimulated chloride secretion in a human colonic epithelial cell line. Gastroenterology 86: 1042, 1984.

8. Cartwright, C., McRoberts, J., Mandel, K., Masui, H. and Dharmsathaphorn, K.: Stimulation of Cl- secretion in a human colonic epithelial cell line. Clin. Res. 32: 279A, 1984.

9. McRoberts, J.A. and Dharmsathaphorn, K.: Ba++-sensitive K+ transport by a human colonic epithelial cell line. Fed. Proc. 43: 447, 1984.

10. Beuerlein, G., McRoberts, J.A., Mandel, K.G., Foster, E. and Dharmsathaphorn, K.: Requirement for a basolateral membrane K+ recycling mechanism in the electrogenic Cl- secretory process. Fed. Proc. 44: 647, 1985.

11. McRoberts, J.A., Beuerlein, G. and Dharmsathaphorn, K.: Evidence for cAMP and Ca++-activated K+ channels in a human colonic epithelial cell line. Fed. Proc. 44: 646, 1985.

12. Mandel, K.G., McRoberts, J.A. and Dharmsathaphorn, K.: Stimulation of an apical membrane Cl- transport pathway by vasoactive intestinal polypeptide. Fed. Proc. 44: 1365, 1985.

13. Dharmsathaphorn, K., Weymer, A. and McRoberts, J.A.: Chloride secretion induced by prostaglandin E1 (PGE1): Participation of Na+,K+,Cl- cotransport, Cl- channels and K+ channels. Gastroenterology 88: 1365, 1985.

14. Dharmsathaphorn, K., Pandol, S. and McRoberts, J.A.: Cl- secretion induced by carbachol in a human colonic epithelial cell line: Studies of the mechanism of action. Gastroenterology 88: 1364, 1985.

15. McRoberts, J.A., Giesen-Crouse, E. and Dharmsathaphorn, K.: Coordinate expression of piretanide receptors and Na+,K+,Cl- cotransport activity in MDCK cell mutants. Fed. Proc. 46: 636, 1987.

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16. Giesen-Crouse, E., McRoberts, J.A. and Dharmsathaphorn, K.: Self-regulation of Na+,K+,Cl- cotransport in MDCK cells. Fed. Proc. 46: 635, 1987.

17. Lira, M., Schteingart, C., Lambert, K. J., McRoberts, J. A., Hofmann, A. F.: Active absorption of monosaccarides by biliary ductules in the perfused rat liver. Hepatology 8: 1452, 1988.

18. Ennes, H.E., McRoberts, J.A., Hyman, Snape, W.J.,Jr.: Effect of cell culture on smooth muscle muscarinic receptors. Gastroenterology 96: A141, 1989.

19. McRoberts, J.A., Aranda, R., Riley, N., Kang, H.: Insulin and insulin-like growth factors regulate the paracellular permeability of T84 human colonic epithelial cells. Gastroenterology 96: A337, 1989.

20. Ennes, H.S., J.A. McRoberts, P.E. Hyman, V. Eysselein, W.J. Snape, Jr. Effect of culture on expression of colonic smooth muscle receptors. Clin. Res. 38: 101A, 1990.

21. Ennes, H.S., J.A. McRoberts, P.E. Hyman, V. Eysselein, W.J. Snape, Jr. Effect of culture on expression of colonic smooth muscle vasoactive intestinal peptide (VIP) receptors. Gastroenterology, 98: A972, 1990.

22. Yuan, O.X., J. Lakshmanan, J.A. McRoberts, W.J. Snape, Jr., P.E. Hyman. Newborn rabbit gastric smooth muscle culture: EGF and TGF, but not IGF I, are mitogens. Gastroenterology 100: A555, 1991.

23. McRoberts, J.A., N. Riley, H. Kang. Insulin, insulin-like growth factors and interferon- increase T84 cell monolayer paracellular permeability through similar mechanisms. Gastroenterology 100: A697, 1991.

24. McRoberts, J.A., N. Riley, L.R. Forte. E. Coli heat stable eneterotoxin mediates chloride secretion through cGMP and protein kinase A. Gastroenterology 102: A226, 1992.

25. Tomomasa, T., Y. Xie, A. Morikawa, H. Mochizuki, J. McRoberts, W.J. Snape, Jr., P.E. Hyman. Developmental changes in morphometry and actinomysin content of rabbit gastric muscle. Gastroenterology 102: A527, 1992.

26. Ennes, H., M. Sottili, Y. Xie, V. Eysselein, W.J. Snape, Jr, J.A. McRoberts. Density dependent EGF receptor expression and mitogenic effect on cultured colonic smooth muscle cells. Gastroenterology 102: A730, 1992.

27. Sottili, M., J.A. McRoberts, M. Reinshagen, A. Patel, E. Lezocbe, V.E. Eysselein. Up-regulation of the epidermal growth factor/transforming growth factor -receptor in T84 colonic epithelial cells by interleukin 1. Gastroenterology 102: A759, 1992.

28. Xie, Y.N., H. Ennes, J. McRoberts, W.J. Snape, Jr. Inositol (1,4,5)-triphosphate is a second messenger in colonic smooth muscle response to Substance P. Gastroenterology 102: A767, 1992.

29. Hoffmann, P., M.J. Domek, F. Procaccino, M. Reinshagen, L. Lui, L. Barajas, J. McRoberts, A. Patel, J. Lakshmanan, S. French, F.W. Leung, V.E. Eysselein. Up-regulation of EGF-binding sites in dextran sulfate induced rat colitis. Gastroenterology, 104: A625, 1993.

30. Reinshagen, M., F. Procaccino, J.A. McRoberts, J. Lakshmanan, M. Sottili, H. Ennes, A. Patel, M. Hierro, S. French, L. Lui, L. Barajas, V.E. Eysselein. Up-regulation of EGF-binding sites in rat colitis. Gastroenterology, 104: A642, 1993.

31. Bortolami, M., H.S. Ennes, J.A. McRoberts, W.J. Snape, Jr., F. Cominelli. IL-1 enhances PGE2 production in rabbit colonic smooth muscle cells (SMC) through a type I IL-1 receptor. Gastroenterology, 104: A672, 1993.

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32. Ennes, H.S., M. Kajimura, J.A. McRoberts, G. Sachs, V.E. Eysselein, W.J. Snape, Jr. Gene expression of muscarinic receptor subtypes in colonic smooth muscle cells. Gastroenterology, 104: A822, 1993.

33. Zeeh, J.M., P. Hoffmann, M. Sottili, V.E. Eysselein and J.A. McRoberts. Increase in insulinlike growth factor I binding sites in the muscularis propriae of inflamed rat colon. FASEB J., 8: A680, 1994.

34. McRoberts, J.A. and N.E. Riley. Modulation of growth factor and cytokine-induced increase in T84 epithelial cell monolayer permeability by media components. FASEB J., 8: A18, 1994.

35. Ambe, R.K., F. Procaccino, J.M. Zeeh, P. Hoffmann, J.A. McRoberts, J. Laksmanan, S.K. Peng, and V.E. Eysselein. Effect of epidermal growth factor on colonic mucus production. Gastroenterology, 106: A594, 1994.

36. Hoffmann, P., J.M. Zeeh, J. Laksmanan, M. Reinshagen, F. Procaccino, J.A. McRoberts, L. Lui, L. Barajas, and V.E. Eysselein. Increased expression of distinct molecular weight glycoproteins in experimental rat colitis. Gastroenterology, 106: A699, 1994.

37. Procaccino, F., M. Reinshagen, P. Hoffmann, J.M. Zeeh, J. Laksmanan, J.A. McRoberts, A. Patel, and V.E. Eysselein. Epidermal growth factor protects against mucosal injury in an experimental model of colitis in rats. Gastroenterology,106: A628, 1994.

38. Zeeh, J.M., H. Ennes, P. Hoffmann, F. Procaccino, V.E. Eysselein, W.J. Snape, Jr, and J.A. McRoberts. Cultured rat colonic smooth muscle cells express insulinlike growth factor-I receptors and IGF binding proteins. Gastroenterology, 106: A853, 1994.

39. Zeeh, J.M., P. Hoffmann, S.L. Aukerman, F. Procaccino, S. Soltani, J.A. McRoberts, G.F. Pierce and V.E. Eysselein. Keratinocyte growth factor improves healing in an experimental model of colitis in rats. Gastroenterology, 106: A853, 1994.

40. Zeeh, J.M., P. Hoffmann, M. Sottili, H. Ennes, V.E. Eysselein, J.A. McRoberts. Up-regulation of insulinlike growth factor binding proteins in experimental colitis in rats. Gastroenterology, 106: A853, 1994.

41. McRoberts, J.A. The combination of insulin-like growth factor and interferon- produces a more than additive increase in paracellular permeability across T84 cell monolayers. Gastroenterology, 108:A307, 1995.

42. Zeeh, J.M., N. Mohapatra, P.K. Lund, V.E. Eysselein, J.A. McRoberts. Differential expression and localization of IGF-I and IGF-I binding protein mRNA in inflamed colon. Gastroenterology, 108:A948, 1995.

43. Zeeh, J.M., F. Procaccino, P. Hoffmann, V.E. Eysselein, J.A. McRoberts. Effects of insulin-like growth factor-I on colonic epithelial proliferation and on mucosal injury in rat colitis. Gastroenterology, 108:A1019, 1995.

44. Zeeh, J.M., F. Procaccino, P. Hoffmann, T.T. Zittel, V.E. Eysselein, J.A. McRoberts. Colonic smooth muscle of rats is a major target of insulin-like growth factor-I in vivo and in vitro. Gastroenterology, 108:A1019, 1995.

45. Zeeh, J.M., P. Hoffmann, S.L. Aukerman, F. Procaccino, J.A. McRoberts, S. Soltani, G.F. Pierce, J. Lakshmanan, D. Lacey, V.E. Eysselein. Effects of keratinocyte growth factor on mucosal injury in rat colitis: A dose response study. Gastroenterology, 108:A948, 1995.

46. Procaccino, F., J.M. Zeeh, T.T. Pizarro, F. Cominelli, P. Hoffmann, J. Lakshmanan, J.A. McRoberts, V.E. Eysselein. Effects of epidermal growth factor on inducible cylcooxygenase and nitric oxide synthetase in rat colonic tissue. Gastroenterology, 108:A897, 1995.

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47. Procaccino, F., J.M. Zeeh, P. Hoffmann, J. Lakshmanan, J.A. McRoberts, V.E. Eysselein. Epidermal growth factor does not accelerate healing in an experimental model of colitis in rats. Gastroenterology, 108:A897, 1995.

48. Wu, S.V., M. Yang, R. Seensalu, J. McRoberts, J.H. Walsh. First intracellular loop of CCK-A receptor is essential for cAMP response to CCK in transfected HEK-293 cells. Gastroenterology, 110: A1133, 1996.

49. Fitzsimmons, T., J.A. McRoberts, K. Tachiki, S. Pandol. Role of fatty acid-CoA esters in Ca2+ signaling in the pancreatic acinar cell. Gastroenterology, 114: A457, 1998.

50. Ennes HS, SH Young, J Goliger, J. McRoberts, EA Mayer. Gap junction-mediated intercellular communication between DRG neurons and three target cell types. Gastroenterology, 114: A1141, 1998.

51. Chaban V, SH Young, HS Ennes, J McRoberts, EA Mayer. Evidence for modulation of mechanosensitivity by endogenous nitric oxide production in cultured DRG neurons. Gastroenterology, 116: A1038, 1999.

52. McRoberts JA, Chaban VV, Ennes HS, Marvizon JC, Mayer EA. Expression of NMDA receptors on adult rat DRG neurons in primary culture. Gastroenterology 118: A1189, 2000.

53. Abreu MT, Palladino AA, Arnold ET, Kwon RS, McRoberts JA. Resiliency of a model human intestinal epithelium to Fas-mediated apoptotic injury, evidence for junctional restructuring in its repair. Gastroenterology 118: A802-A803, 2000.

54. Chaban VV, Ennes HS, McRoberts JA, Mayer EA. Nitric oxide synthase inhibitors enhance mechanosensitive Ca2+ influx in cultured DRG neurons. Gastroenterology 118: A858-A859, 2000.

55. Tognetto M, Amadesi S, Creminon C, Geppetti P, McRoberts JA, Mayer EA. Stimulation of NMDA receptors causes release of CGRP from the peripheral terminals of extrinsic primary afferent neurons. Gastroenterology 118: A1201, 2000.

56. McRoberts, J.A., H.S. Ennes, V.V. Chaban, H.E. Raybould, E.A. Mayer. Expression of NMDA receptors on dorsal root ganglia neurons in primary culture. Society for Neuroscience Abstracts 30: 935, 2000.

57. Marvizon, J.C., J.A. McRoberts, H.S. Ennes, T. Johansson, B. Corneliussen, L. Jinton, E.A. Mayer. Expression of NMDA receptor subunits in different types of primary afferent neurons. Gastroenterology, 120:A331, 2001.

58. Wei, J.Y., Y.H. Wang, J.A. McRoberts, E.A. Mayer. An in vitro distal colon-splanchnic nerve preparation: Single unit responses to colorectal distension and bradykinin. Gastroenterology, 120: A328, 2001.

59. Wei, J.Y., Y.H. Wang, E.A. Mayer, J.A. McRoberts. Role of peripheral NMDA receptors in colorectal distension mediated afferent nerve activity: An in vitro study. Gastroenterology, 120: A329, 2001.

60. McRoberts, J.A., H.S. Ennes, V.V. Chaban, Wei, J.Y., Y.H. Wang, E.A. Mayer. Potentiation of mechanical responses by NMDA in DRG neurons and spinal afferents. Society for Neuroscience Abstracts, Program No. 53.11, 2001.

61. Marvizón, J.C.G. , J.A. McRoberts, H.S. Ennes, B. Song, X. Wang, L. Jinton, B. Corneliussen, E.A. Mayer. Subunit composition of NMDA receptors in rat dorsal root ganglia. Society for Neuroscience Abstracts, Program No. 819.12, 2001.

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62. McRoberts JA, Chaban VV, Ennes HS, Waldron RT, Rozengurt E, Mayer E. The glutamate receptor agonist NMDA potentiates mechanosensitive Ca2+ responses of rat DRG neurons through protein kinase C. Gastroenterology 122:A43, 2002.

63. McRoberts JA, Gale G, Schwetz I, Sablad M, Miller J, Zhou H, Fanselow M, Plotsky PM, Mayer EA. Relationship between behavioral characteristics and stress-induced visceral hyperalgesia in response to colorectal distension following neonatal maternal separation in Long Evans rats. Gastroenterology 122:A157, 2002.

64. Million M, Coutinho SV, Sablad M, McRoberts JA, Mayer EA, Rivier J, Vale W, Taché Y. Human urocortine II (hUcn II), a new selective CRF2 agonist, prevents colorectal distention-induced visceral pain in rats. Gastroenterology 122:A257, 2002.

65. Wang YH, Wei JY, Mayer EA, McRoberts JA. The glutamate receptor agonist NMDA dose-dependently increases the basal activity and alters the colorectal distension responsiveness of rat inferior splanchnic afferents in vitro. Gastroenterology 122:A408, 2002.

66. Wang YH, McRoberts JA, Mayer EA, Wei JY. Characterization of colorectal distension-responsive polymodal afferents of the rat inferior splanchnic nerve in vitro. Gastroenterology 122:A410, 2002.

67. Million M, McRoberts JA, Mayer EA, Grigoriadis D, Taché Y. A novel water soluble CRF1 antagonist prevents stress-induced visceral hyperalgesia and colonic motor response in rats. Gastroenterology 122:A527, 2002.

68. Schwetz I, McRoberts JA, Bradesi S, Miller JC, Zhou H, Mayer EA. NK1 receptors are involved in delayed stress induced visceral hyperalgesia in Wistar rats. Soc Neurosci Abstr 2002.

69. McRoberts JA, Ennes HS, Gale GD, Schwetz I, Zhou H, Miller JC, Fanselow M, Plotsky PM, Mayer EA. Association of anxiety and increased colonic motility with stress-induced visceral hyperalgesia n maternally separated Long Evans rats. Soc Neurosci Abstr 2002.

70. Wang Y, Wei J, McRoberts JA, Mayer EA. NMDA modulates the mechanosensitivity of rat inferior splanchnic afferents in vitro. Soc Neurosci Abstr 2002.

71. Bradesi S, Schwetz I, McRoberts J, Ohning G, Mayer EA. Chronic water avoidance stress induces visceral hypersensitivity in male Wistar rats. Gastroenterology 124:A671, 2003.

72. Li J, McRoberts JA, Ennes HS, Nie J, Mayer EA. Functional characteristic of NMDA receptors in DRG neurons from rats with and without colitis. Gastroenterology 124:A116, 2003.

73. McRoberts JA, Ennes HS, Li J, Slice L, Mayer EA. Rat DRG neurons express NR3 NMDA receptor subunits and excitatory glycine receptors. Gastroenterology 124:A468, 2003.

74. Schwetz I, McRoberts JA, Coutinho SV, Bradesi S, Mulugeta M, Miller JC, Zhou H, Ohning G, Mayer EA. CRF1 receptor mediates acute and sustained visceral hyperalgesia following an acute stressor in maternally separated Long Evans rats. Gastroenterology 124:A612, 2003.

75. Wang Y, Wei JY, McRoberts JA, Mayer EA. The effect of Mg2+ and D-serine on the NMDA responsiveness of rat inferior splanchnic afferents in vitro. Gastroenterology 124:A249, 2003.

76. Li J, McRoberts JA, Ennes H, Mayer EA. Experimental colitis alters the functional properties of NMDA receptors expressed by colon-specific DRG neurons in rats. Gastroenterology 126: A-162, 2004.

77. McRoberts JA, Wang Y, Mayer EA. N-methyl-D-aspartate (NMDA) receptors modulate the response of inferior splanchnic afferent fibers to colorectal distension. Gastroenterology 126: A-162, 2004.

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78. Amadesi S, Nie J, Vergnolle N, Cottrell GS, Grady EF, Trevisani M, Manni C, Geppetti P, McRoberts JA, Davis JB, Mayer EA, Bunnett NW. Protease-activated receptor 2 (PAR2) sensitizes the capsaicin receptor TRPV1 to induce hyperalgesia. Gastroenterology 126: A-427, 2004.

79. Bradesi S, Patierno S, Ennes H, McRoberts J, Ohning G, Sternini C, Pothoulakis H, Mayer EA. Visceral hypersensitivity induced by chronic stress in male Wistar rats: Regulation of colonic neurokinin 1 receptors and pro-inflammatory cytokines. Gastroenterology 126: A-93, 2004.

80. Wang Y, McRoberts JA, Mayer EA. The glutamate analogue, N-methyl-D-aspartate (NMDA), non-specifically activates inferior splanchnic afferent fibers innervating the distal colon. Gastroenterology 126: A-482, 2004.

81. Bradesi S, Schwetz I, McRoberts J, Ohning G, McLean PG, Winchester WJ, Hicks GA, Mayer EA. Effect of acute stress on visceral sensitivity after previous chronic stressful experience. Gastroenterology 126: A-591, 2004.

82. Li J; McRoberts JA; Ennes HS; Mayer EA. Modulation of NMDA receptors by phosphorylation in adult rat DRG neurons. Program No. 861.7. 2004 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience.

83. Li J, Mayer EA, Ennes HS, McRoberts JA. Modulation of NMDA receptors by SRC family tyrosine kinases in adult rat DRG neurons. Gastroenterology 128:A-223, 2005

84. Li J, McRoberts JA, Ennes HS, Mayer EA. Non-genomic modulation of NMDA receptors by estrogen in adulat rat DRG neurons. Gastroenterology 128:A-222, 2005.

85. Larauche M, Mayer EA, Bradesi S, Ohning GV, Tache Y, McRoberts JA. Corticotropin releasing factor-1 receptors (CRF1Rs) only partially mediate the sustained visceral hyperalgesia induced by repeated psychological stressor in rodents. Gastroenterology 128:A-86, 2005.

86. Bradesi S, Kokkotou E, Song B, Marvizon JC, Mittal Y, McRoberts JA, Ennes HS, G. Ohning GV, Pothoulakis C, Mayer EA. Sustained visceral hyperalgesia following chronic psychological stress in rats involves upregulation of spinal NK1 receptors. Gastroenterology 128:A-494, 2005.

87. Li J, JA McRoberts, HS Ennes, EA Mayer. Sex-dependent differences in the modulation of NMDA receptors by estrogen in rat DRG neurons. Program No. 860.5, 005 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2005.

88. McRoberts JA, HS Ennes, JC Marvizon, Y Mittal, B Vissel, M Fanselow, EA Mayer. Targeted knock down of NMDA receptors in dorsal root ganglia neurons. Gastroenterology 130:A-337, 2006

89. Adelson D, JA McRoberts, Y Wang, EA Mayer. NMDA receptors differentially regulate the excitability of distinct classes of splanchnic afferent neurons innervating the distal rat distal colon. Gastroenterology 130:A-255, 2006.

90. McRoberts JA, HS Ennes, JC Marvizon, Y Mittal, B Vissel, M Fanselow, EA Mayer. Knock down of NMDA receptors in dorsal root ganglia neurons decreases inflammatory hyperalgesia to formalin injection. Program No. 704.3, 2006 Neuroscience Meeting Planner. Atlanta, GA: Society for Neuroscience, 2006.

91. McRoberts JA, CD Bryant, N Zhang, HS Ennes, B Vissel, M Fanselow, EA Mayer. Differential nociceptive responses to thermal and mechanical stimuli in the closely related mouse substrains C57BL/6J and C57BL/6N. Program No. 72.10 2007 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2007.

92. McRoberts JA, Ennes HS, Ameen VZ, Sanger GJ and Mayer EA. Regulation of CGRP Release by 5-HT3 Receptors. Gastroenterology, 134:A708, 2008.

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93. Ennes HS, McRoberts JA, Hicks G, Pfannkuche H-J, Mayer EA. Expression of 5-HT4 receptor splice variants by DRG neurons. Gastroenterology, 134:A709, 2008.

94. McRoberts JA, HS Ennes, JC Marvizon, Y Mittal, B Vissel, M Fanselow, EA Mayer . Tissue specific knockdown of NMDA receptors in primary afferent neurons decreases inflammatory hyperalgesia. Gastroenterology, 134:A556, 2008.

95. Ennes HS, Nguyen B, Mayer EA, McRoberts JA. Sex-dependent differences in nociceptive responses to peripheral NMDA receptor activation. Gastroenterology, 134:A560, 2008.

96. McRoberts JA, Ennes HS, Pham H, Slice LW, Mayer EA, Bradesi S. Up-regulation of COX-2 expression in the spinal cord and dorsal root ganglia of rats exposed to chronic psychological stress. Gastroenterology,136, A-724, 2009.

97. Bradesi S, Ennes HS, Karagiannidis I, Kyriaki B, McRoberts JA, Pothoulakis C, Mayer EA. Modulation of Spinal Glia Activation and Glutamate Transmission in the Model of Chronic Water Avoidance Stress-Induced Visceral Hyperalgesia: Role of Glucocorticoids. Gastroenterology, 138, S-278, 2010.

98. McRoberts JA, Jagannathan S, Ennes, Marvizon JC, Mayer EA, Fanselow M, Wood JN, Vissel B. Tissue-selective knockout of NMDA receptors in nociceptive afferents abolishes peripheral sensitization to NMDA receptor agonists. Program No. 581.06. 2011 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2011.

99. Marvizon JC, Chen W, McRoberts JA. BDNF triggers the activation by Src family kinase phosphorylation of the NMDA receptors that induce substance P release from primary afferents. Program No. 581.06. 2011 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2011.

100.Chen W., McRoberts J.A., Marvizon J.C.G.Mu-opioid receptors switch NMDA receptors in primary afferents to a non-functional state. Program No. 882.19, 2012 Neuroscience Meeting Planner, Society for Neuroscience, 2012.

101.McRoberts JA, Walwyn W, Ennes HS, Chen W, Marvizon JC. BDNF increases NMDA receptor currents in primary afferent neurons by inducing phosphorylation of Tyr1472 of the NR2B subunit. Program No. 267.03, 2013 Neuroscience Meeting Planner, Society for Neuroscience, 2013

102.Marvizon JC, Chen W, Walwyn W, Ennes H, McRoberts JA. NMDA receptors in primary afferents are potentiated by BDNF released by microglia during the induction of neuropathic pain, Program No. 267.02, 2013 Neuroscience Meeting Planner, Society for Neuroscience, 2013.

103.Chen W, McRoberts JA, Marvizon JC. Mu-opioid receptor inhibition of substance P release from primary afferents disappears in neuropathic pain but not in inflammatory pain. 2013 Neuroscience Meeting Planner, Society for Neuroscience, 2013

104.Ennes H, Chen W, McRoberts JA, Marvizon JC. Interleukin-1β induces substance P release from primary afferent terminals independently of NMDA receptors. Program No. 741.06, 2013 Neuroscience Meeting Planner, Society for Neuroscience, 2013.

105.Walwyn W, Kim H, Ennes HS, Chen W, McRoberts JA, Marvizon JC. Constitutive activity of µ-opioid receptors suppresses hyperalgesia in complete Freund’s adjuvant-induced Latent Sensitization. Program No. 534.20, 2014 Neuroscience Meeting Planner, Society for Neuroscience, 2014

106.Marvizon JC, Kim H, Walwyn W, Minasyan A, Ennes HS, Chen W, McRoberts JA. µ-Opioid receptor knock-out mice only partially recover from hyperalgesia in Latent Sensitization - contribution of α2A adrenergic and δ-opioid receptors to pain remission. Program No. 730.17, 2014 Neuroscience Meeting Planner, Society for Neuroscience, 2014.

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107.McRoberts JA, Kim H, Minasyan A, Chen W, Ennes HS, Walwyn W, Marvizon JC. Latent Sensitization of neuropathic pain in rats and mice - involvement of µ-opioid receptors. Program No. 730.16, 2014 Neuroscience Meeting Planner, Society for Neuroscience, 2014.

108.Chen W, Kim H, Ennes HS, Walwyn W, McRoberts JA, Marvizon JC. NMDA receptor-induced substance P release from primary afferent terminals is inhibited by µ and κ, but not δ, opioid receptors. Program No. 534.06, 2014 Neuroscience Meeting Planner, Society for Neuroscience, 2014.

109.Kim H, Chen W, Ennes HS, Walwyn W, McRoberts JA, Marvizon JC. NMDA receptor-induced substance P release from primary afferent terminals is inhibited by α2A adrenergic receptors, lack of synergism with µ-opioid receptors. Program No. 534.20; 2014 Neuroscience Meeting Planner, Society for Neuroscience, 2014.

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