Concentrations of Anti-RON ADCs (μg/ml)

Ce

ll v

iab

ilit

y (

% o

f c

on

tro

l)

IC50 (μg/ml) Zt/g4-DM1: 1.65 Zt/c1-DM1: 6.24

LoVo: RON per cell <10

DLD1: RON per cell ~4,480

HT29: RON per cell ~18,797

IC50 (μg/ml) Zt/g4-DM1: 4.03 Zt/c1-DM1: 4.36

IC50 (μg/ml) Zt/g4-DM1: 20.36 Zt/c1-DM1: 21.14

IC50 (μg/ml) Zt/g4-DM1: >100 Zt/c1-DM1: >100

SW620: RON per cell ~11,256

0

20

40

60

80

100

0 3 6 9 12 15

Feng et al. Supplementary Fig. 3

Caption: Effect of Zt/g4-DM1 on reduction of cell viability in CRC cell lines expressing different numbers of RON receptor