conflict of interest disclosure, has no real or apparent conflicts of interest to report
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Conflict of Interest Disclosure <Hidezo> <Mori>, <MD>
Has no real or apparent
conflicts of interest to report.
Visualization of Perforating Branches of Cerebral Arteries Toward Clinical Evaluation of Vascular Disease and Alzheimer’s-overlap
Syndrome
Hidezo Mori1, Naoto Fukuyama1, Yoshimori Ikeya1, Toshiharu Fujii1, Chiharu Tanaka1, Yuko Tsukamoto1, Shunya Takizawa1, Keiji Umetani 2.
1Departments of Physiology, Neurology and Cardiology, Tokai University School of Medicine, Ishehara, 2Division of Reserch and Utilization, JASRI, Sayo-cho, Japan
BackgroundVascular cognitive impairment (VCI) is characterized By sub-corticalwhite-matter lesion on MRI/CT, and often associated with Alzheimer Disease.
Purpose of Present Study
The present study examined whether
synchrotron radiation (SR) microangiography
can detect functional DM microangiopathy
and visualize directly the perforating
branches of cerebral arteries (PBCA) in rat
models.
An illustration of an experimental arrangementAn illustration of an experimental arrangement for synchrotron microangiography in ratsfor synchrotron microangiography in rats
Bending magnet
Synchrotron radiation
Single-clystal monochromator
Monochromatic X-Ray
object
X-Ray SaticonCamera
8GeV Electron Beam
Slitt
X-ray Shutter
線形加速器(0 ⇒1 GeV)
Synchrotron (1 ⇒ 8 GeV)
A
B
Langendorf
MI heart
Mass
att
enuation c
oeffi
cient (c
m2/g
)
Photon energy (KeV)0 20 40 60 80 100 120 140
1000
100
10
1
0.1
bone
筋 肉
ヨード
K 吸収端
M
ass
abso
rptio
n co
effic
ient
Photon energy
K-edge
iodine
bone
muscle
(cm2/kg)
( keV )
Conventional white x-ray
Monochromatic x-ray
Mass Absorption Coefficient Plotted Mass Absorption Coefficient Plotted Against Photon Energy Against Photon Energy
Protocol-1 • In order to determine whether fingertip synchrotron
radiation (SR) microangiography has enough spatial resolution to quantitate arterioles (20-200 μm in diameter) , we measured diameter reduction as arteriolar branching in fingertip microvessels.
• Next, we compared the arteriolar diameter changes induced by Ach administration between control and DM rats.
b-2
a-1 a-2
100 μm
Microvessel of Fingertip
b-1
*‡
#
‡ Daughter 1
* Mother
# Daughter 2
Baseline Ach
Lu
men
Dia
met
er o
f 1s
t D
aug
hte
r A
rter
y
Mother and 1st Daughter Artery
n = 38
Lu
men
Dia
met
er o
f 2n
d D
aug
hte
r A
rter
y
Mother and 2nd Daughter Artery
Baseline Ach Stress
Normal Group DM Group
n = 25 n = 37
Baseline Ach Stress
142.4 ± 61.9 190.9 ± 73.5 201.6 ± 83.0 160.4 ± 67.9
P < 0.016 P < 0.024
Protocol-2
• Whether SR microangiography can visualize perforating branches of cerebral arteries (PBCA) in rat models.
• By injecting contrast materials selectively into internal carotid artery SR microangiography was applied to visulaize cerebral microvessles in control and DM rats.
Perforating branches of cerebral arteries in normal ratPerforating branches of cerebral arteries in normal rat
Perforating branches of cerebral arteries in DM ratPerforating branches of cerebral arteries in DM rat
ConclusionConclusion
SR microangiography can detect functional
DM microangiopathy the both in fingertip arterioles
and perforating branches of cerebral arteries in
rats, and could be useful for clinical evaluation
of vascular disease and Alzheimer’s-overlap
syndrome.