csiv-tre-rfa-ubc-kt 12464 bp 1 plasmid name: csiv-tre-rfa
TRANSCRIPT
CSIV-TRE-RfA-UbC-KT12464 bp
MluI 228
NotI 1521
EcoRI 2565
NheI 2905NotI 3039BamHI 3110BspEI 3356EcoRI 3360
MluI 3807BamHI 3813
PstI 4482AgeI 4625
XhoI 4631
AgeI 5878XbaI 6600ApaI 6742BamHI 7352XbaI 7358HpaI 7364
EcoRV 8212BspEI 8252
ApaI 8446PmeI 8452
CMV R U55' SD
RRE
3' SAcPPT+CTS
TRE P
RfA
attR1
Cm
ccdBattR2
UbChKO1
T2ArtTA
PREdel U3
loxPRU5
BGH pAf1 oriSV40 pro & ori
ZeoSV40 pA
ColE1 ori
Amp 1
Miyoshi
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Plasmid name: CSIV-TRE-RfA-EF-KT Plasmid size: 12404 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence TRE P: Tet-responsive promoter (TRE-Tight) RfA: Gateway Reading Frame Cassette A containing Cm, ccdB, attR1, and attR2 Cm: Chloramphenicol resistance gene ccdB: The ccdB gene encodes a protein that interferes with E. coli DNA gyrase attR1, 2: Sites for site-specific recombination with a Gateway vector (Invitrogen). attR sites always recombine with attL sites in a reaction mediated by the LR Clonase (Invitrogen). UbC: Human ubiquitin C promoter hKO1: Humanized Kusabira-Orange T2A: Thosea asigna virus 2A peptide sequence rtTA: Reverse Tet-controlled transactivator protein (Tet-On-Advanced) PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region loxP: Site-specific recombination site for Cre recombinase BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene *Use the DB3.1 or ccdB Survival 2 T1R E. coli strain for propagation of plasmids containing the ccdB gene.
Miyoshi
テキストボックス
y