CTGAATGGCT TAAAATAATC TTTTTAATTA CAATAAATTA GAACTAAAAA TCGTCACAGA TCATTAGATCGACTTACCGA ATTTTATTAG AAAAATTAAT GTTATTTAAT CTTGATTTTT AGCAGTGTCT AGTAATCTAG

ACTCTAATCA TATTTAATCA TTTAAATCAG AAAGATCAGT TATTTAAAAA CAACAAATTT TTCTTTATTTTGAGATTAGT ATAAATTAGT AAATTTAGTC TTTCTAGTCA ATAAATTTTT GTTGTTTAAA AAGAAATAAA

ATGATCTCTT TTTCTTTATT CTCTTGGAAC TATAGTGATA TTACGGTAAG TGTGATACGG ATCTAACCATGTACTAGAGAA AAAGAAATAA GAGAACCTTG ATATCACTAT AATGCCATTC ACACTATGCC TAGATTGGTAC

-35

-10

924

Figure S1

Supplementary Figure Legend

Fig. S1: Nucleotide sequence of the ig2 fragment in reporter B1. B1 starts from TIGR bp 924. The region highlighted in grey is the putative IHF binding site and GATC sites for Dam methylation are underlined. The predicted -10 and -35 regions of PrctB are outlined in black. The start site of rctB transcription is in bold. The rctB start codon is in bold and underlined.