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Abhishek Kumar Mishra (PhD)
(1/6/1982)
Corresponding address: 4040, Department of Pharmacology, AIIMS, New Delhi-29, India
Email and LinkedIn: [email protected]
Contact No. and Skype address: +91-8447197715, 9958298072 [email protected]
Objective: To promote the goodwill of the institute for better output through a disciplined,
organized and progressive ways with my sincere hard work and utmost endeavours in the task
entrusted to me and keep growing at every step of my life.
Academia
Year Degree/Result University/ Institution Marks (%)
February
2015 PhD
All India institute of medical
sciences, New Delhi Awarded
July 2006 M.Sc.
(Biotechnology) Punjabi university, Patiala (Punjab) 68.45
July 2000 BSc. (Life Science)
St. Andrews P G college, Gorakhpur 59.94
PhD thesis and Research projects
PhD Thesis Title: Development of small interfering RNAs (siRNAs) against
Coxsackievirus A24 causing eye infections
Senior Research Fellow in an AYUSH, Ministry of Health and family welfare funded
project “Preliminary pharmacological and safety studies of Homeopathic drugs” in the
department of Pharmacology, AIIMS, New Delhi (9/2/2015 to 12/06/2015).
Senior Research Fellow in an Indian Council of Medical Research funded and awarded
research project “Microsporidial keratoconjunctivitis in Delhi: A prospective study
using rapid molecular diagnostics and cytology” in the Department of Ocular
Microbiology, All India Institute of Medical Sciences, New Delhi, (February, 2013 to
February, 2015)
Senior Research Fellow in Department of Biotechnology funded research project
“Development of small interfering RNA (siRNA) against Enterovirus70 and
CoxsackievirusA24 causing acute haemorrhagic conjunctivitis” in the Department of
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Ocular Microbiology, All India Institute of Medical Sciences, New Delhi (March, 2010 to
July ,2012)
Junior Research Fellow in an Indian Council of Medical Research funded research project
“Evaluation of polymerase chain reaction assay for Herpes Simplex Virus-I (HSV)
detection in tear specimen of suspected viral keratitis, particularly stromal and
atypical epithelial keratitis.” in the Department of Ocular Microbiology, All India
Institute of Medical Sciences, New Delhi (July,2007 to March,2010)
CSIR-UGC NET(Life Science) Qualified (310296/23/12/2007)
Trainee in Biotech Consortium India Limited sponsored training programme in the area of
“Human Papillomavirus Detection assay” (29.11.06 -28.05.07)
Masters project “Cytotoxicity assay of lung cancer cell line -A549 by using m-PEG-
DAAO conjugates” at Punjabi University, Patiala, India (01.12.05 - 01.05.06)
Trainee in CSIR sponsored CIMAP summer school on “Molecular techniques in Bio
prospection and biodiversity analysis” at Central Institute of Medicinal and Aromatic
Plants, Lucknow, India (15.06.05 -30.07.05)
Scientific and technical skills
Working micro-organisms: Mycobacterium tuberculosis, Acanthamoeba, Coxsackie
virus A24, Human Enterovirus 70, Human Adenovirus, Microsporidia, Herpes simplex
virus-I, Human Cytomegalovirus and Chlamydia trachomatis.
Molecular Biology: PCR, Molecular cloning, Western, Northern and Southern blotting,
in vitro transcription, Interferon assay, RNA interference experiments and other
techniques involved in molecular biology experiments, Flow cytometry
Animal cell culture: Media and reagents preparation, Maintenance of cell lines,
Propagation of virus in cell lines, Transfection and cytotoxicity assays.
Microscopy: Bright field, Phase contrast and Immunofluorescence microscopy
Bioinformatics and Biostatistical tools: shRNA and siRNA designing, Primer express,
Bio Edit, Gene runner, Rasmol, ClustalW, MEGA 6.0, IBM SPSS, Graph pad PRISM
Scientific papers
Satpathy G, Mishra AK, Tandon R, Sharma MK, Sharma A, Nayak N, Titiyal JS,
Sharma N. Evaluation of tear samples for Herpes Simplex Virus 1 (HSV) detection in
suspected cases of viral keratitis using PCR assay and conventional laboratory diagnostic
Br J Ophthalmology. 2011 Mar; 95(3):415-8
Development of effective small interfering RNAs (siRNAs) to treat acute hemorrhagic
conjunctivitis in Coxsackie virus A24 infections (Paper communicated)
Outbreak of Adenovirus in a tertiary care hospital of North India-Emergence of a new
ocular strain? (Paper communicated)
Scientific abstracts and presentations
Satpathy G, Patnayak D, Mishra AK and Panda SK. siRNA mediated inhibition of
Entero Virus 70 (EV 70) causing acute haemorrhagic conjunctivitis in tissue culture
system. Presented in 110th General meeting of American Society of Microbiology, May
23rd- 27th, 2010, San Deigo, CA, USA. pp F 2147 (ABSTRACT)
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Satpathy G, Patnayak D, Mishra AK, Panda SK. In vitro inhibition of conjunctivitis
causing Enteroviruses (EV 70 & Coxsackievirus A 24) by RNAi using shRNAs.
Presented in 9th International Symposium on Positive – strand RNA viruses. May 17th –
23rd, 2010, Atlanta, GA, USA. pp 61. (ABSTRACT)
Mishra AK, Satpathy G. Inhibition of Coxsackievirus A24 by sequence- specific small
Interfering RNAs (siRNAs). Presented in ASIA ARVO 2013, October 28-31. New Delhi,
India, pp 92. (POSTER)
Mishra AK, Satpathy G. Development of effective small interfering RNAs (siRNAs) to
treat acute hemorrhagic conjunctivitis by Coxsackie virus A24 infections Presented in
SYSCON on Recent Advances in biological sciences held on 10th December 2014 at All
India Institute of Medical Sciences, New Delhi, India (POSTER)
Other Scientific involvements
Organizing committee member of SIHAM ( March 2010) at All India Institute of
Medical Sciences , New Delhi , India
Organizing committee member of CME (April,2009) at All India Institute of Medical
Sciences , New Delhi , India
Participated in mini international symposium on “Drug discovery and development :
case studies”organised by Department of Pharmacology, All India Institute of Medical
Sciences held on 30th October 2007at AIIMS,New Delhi,India.
Participated in National Symposium on Immunity to Infection organised by SYS-
AIIMS, held on 8th October 2007at All India Institute of Medical Sciences,New
Delhi,India.
Participaed in National Symposium on Nanotechnology in medicine organized and
conducted by SYS- All India Institute of Medical Science ,held on 13 Jan 2007, New
Delhi,India.
Successfully completed a Flow Cytometry BD FACSOrient (BD FACS Calibur) training
programme from 27th-29th May, 2015 at BD-JH FACS Academy, New Delhi.
References
Dr Gita Satpathy
Professor and Head,
Department of Microbiology
All India Institute of Medical Sciences
Ansari Nagar, New Delhi-110029
Email:[email protected]
Phone: 011-26593171
Dr S K Panda
Professor and Head
Department of Pathology
All India institute of Medical Sciences
Ansari Nagar, New Delhi-110029
Email:[email protected]
Phone:011-26594924
Dr Radhika Tandon
Professor of Ophthalmology
Dr R P Centre for Ophthalmic Sciences
All India Institute of Medical Sciences
Ansari Nagar, New Delhi-110029
Email: [email protected]
Phone: 011-26593194
Dr Neelam Verma
Professor of Biotechnology
Department of Biotechnology
Punjabi University, Patiala,
Punjab-147002
Email:[email protected]
Phone: +98-9815710298