千葉大学・大学院医学研究院・助教

科学研究費助成事業　　研究成果報告書

様　式　Ｃ－１９、Ｆ－１９－１、Ｚ－１９ （共通）

機関番号：

研究種目：

課題番号：

研究課題名（和文）

研究代表者

研究課題名（英文）

交付決定額（研究期間全体）：（直接経費）

１２５０１

若手研究(B)

2017～2015

マウス頬ひげ原基誘導と感覚神経回路網のパターン連携に関する研究

Pattern formation between the induction of whiskers and sensory nerves of mice

２０７０７８９８研究者番号：

石田　研太郎（Ishida, Kentaro）

研究期間：

１５Ｋ２１３５２

平成 年 月 日現在３０ ５ ２６

円 3,200,000

研究成果の概要（和文）：本研究は、胎仔期マウスの頬ひげの器官原基誘導と神経線維の侵入・接続を生体外培養系で再現する手法を開発し、感覚器官としての頬ひげが中枢神経と連携する仕組みの解析を目的とした。はじめに、免疫組織化学的な手法により、頬ひげと神経の接続部の細胞集団を解析した。次に、マウス胎仔の頬皮膚と三叉神経節の器官培養法を開発した。そして、これらの共培養により頬ひげと三叉神経の接続を培養皿上で構築する手法を開発した。本研究で得られた知見や培養実験方法は、感覚器官としての頬ひげの成立と三叉神経との接続・連携の仕組みの解明に貢献するものと期待される。

研究成果の概要（英文）：In this study, I aimed to analyze the pattern formation between the induction of whisker follicles and somatosensory nerves through development of organ culture methods in order to observe the formation of a key structure, called Merkel cell-neurite complex, on a dish. I first analyzed the complex between whisker follicles and trigeminal nerves using immunohistochemical techniques. I initially developed two distinct culture methods of a whisker pad and a trigeminal ganglion explant, and then combined them. After the co-cultivation of the whisker row with a trigeminal ganglion explant, a Merkel cell-neurite complex composed of Merkel cells, trigeminal nerve fibers and Schwann cells was observed. My method for the formation of a Merkel cell-neurite complex in an organ culture will contribute to understanding the regulatory mechanisms of the coordination between trigeminal nerves and whisker follicles.

研究分野： 発生生物学

キーワード： 上皮・間葉相互作用　器官培養　頬ひげ原基　三叉神経節　器官形成　Merkel細胞

２版

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6 N Kentaro Ishida & Toshiyuki Mitsui

Role of the boundary in feather bud formation on one-dimensional bioengineered skin APL Bioengineering 2, 016107, 2018

(DOI: 10.1063/1.4989414)

Yuta Kato, Naoto Sakashita, Kentaro Ishida & Toshiyuki Mitsui Gate voltage controlled threading DNA into transistor nanopores The Journal of Physical Chemistry B 122(2), 827-833, 2018 (DOI: 10.1021/acs.jpcb.7b06932)

Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa & Takashi Tsuji Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model Science Advances 2(4), e1500887, 2016

(DOI: 10.1126/sciadv.1500887)

Kentaro Ishida & Toshiyuki Mitsui Generation of bioengineered feather buds on a reconstructed chick skin from dissociated epithelial and mesenchymal cells Development, Growth & Differentiation 58(3), 303-314, 2016 (DOI: 10.1111/dgd.12275)

Naomi Yamamoto, Masamitsu Oshima, Chie Tanaka, Miho Ogawa, Kei Nakajima, Kentaro Ishida, Keiji Moriyama & Takashi Tsuji Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field Scientific Reports 5, 18393, 2015 (DOI: 10.1038/srep18393)

S Manabu Sugimoto, Yuta Kato, Kentaro Ishida, Changbae Hyun, Jiali Li & Toshiyuki Mitsui DNA motion induced by electrokinetic flow near an Au coated nanopore surface as voltage controlled gate Nanotechnology 26(6), 065502, 2015

(DOI: 10.1088/0957-4484/26/6/065502)

38 N Kentaro Ishida & Toshiyuki Mitsui, Feather

bud formation on limited domain of reconstituted artificial skin, 18th International Congress of Developmental Biology, University Cultural Centre, National University of Singapore, 18th - 22nd June 2017

Kentaro Ishida & Toshiyuki Mitsui, Feather bud formation on limited domain of reassembled artificial skin, 50th Annual Meeting of the Japanese Society of Developmental Biologists, Tower Hall Funabori, Tokyo, 10 May 2017

Kentaro Ishida & Toshiyuki Mitsui, Wnt/β-

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feedback loop involving Wnt/β-catenin, FGF/ERK and SOSTDC1 modulates the self-patterning of bioengineered feather buds in a developmental timing-dependent manner, 48th Annual Meeting of the Japanese Society of Developmental Biologists, Tsukuba International Congress Center, Ibaraki, 3 June 2015

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