1. 20141218 KodamaYuichi,Ph.D DDBJ DDBJcenter,annotator 2. 20141218 DDBJ 30DDBJing(JST) INSDC: JGA DDBJ Qualityvalue DRA BioProject BioSample 1 3. 20141218 BioProject/BioSample 30DDBJing(JST) BioSample1BioSample2 data UmbrellaBioProject Genome BioProject Transcriptome BioProject Epigenome BioProject datadatadatadatadata ! ! 2 4. 20141218 SRA 30DDBJing(JST) ! SRAStudyBioProjectSRASampleBioSample ! DDBJSRA(DRA)2014512 3 5. 20141218 30DDBJing(JST) DRARun DRAExperiment Run 1SRA 3. Experiment1Sample ( Sample) TSV TSV BioProject BioSample 1. TSV TSV 2. ExperimentRun 0. (https://trace.ddbj.nig.ac.jp/D-way)D-way DRAcentername http://trace.ddbj.nig.ac.jp/dra/submission.html#DRA_ 4 6. 20141218 DRA3 30DDBJing(JST) ! BioProjectBioSampleDRAExperimentDRARun ! :DRX000001(DRX) 5 7. 20141218 D-way 8. 20141218 D-way 30DDBJing(JST) ! D-way(https://trace.ddbj.nig.ac.jp/D-way/) ! centernameDRA DRA BioProject BioSample centername D-way http://trace.ddbj.nig.ac.jp/book/account.htmlHandbook: centername:SRA : 7 9. 20141218 10. 20141218 30DDBJing(JST) ! BioProjectBioSampleExperimentRun ! 1. 2. http://trace.ddbj.nig.ac.jp/dra/submission.html# : 9 11. 20141218 BioProject 12. 20141218 30DDBJing(JST) ! DDBJ DRA Qualityvalue BioProject 11 13. 20141218 30DDBJing(JST) http://www.ncbi.nlm.nih.gov/bioproject/PRJDA38027 SRA Pubmed NCBIBioProject: 12 14. 20141218 30DDBJing(JST) ! 20141112Projectdatatype 13 15. 20141218 30DDBJing(JST) ! () ! ! DDBJ http://trace.ddbj.nig.ac.jp/bioproject/submission.html# 14 16. 20141218 30DDBJing(JST) ! () ! or() ! PRJDBioProjectID 15 17. 20141218 BioSample 18. 20141218 BioSample 30DDBJing(JST) ! ! ! 20142DDBJBioSample http://trace.ddbj.nig.ac.jp/biosample/index.html 17 19. 20141218 (attributes) 30DDBJing(JST) ! :(:tissue:liver) http://www.ncbi.nlm.nih.gov/biosample/1990977 NCBIBioSample: 18 20. 20141218 30DDBJing(JST) ! (Sampletype) http://trace.ddbj.nig.ac.jp/biosample/attribute.html: Environmentalpackage (16SrRNA) () 19 21. 20141218 30DDBJing(JST) ! Sampletype ! ! or() ! SAMDBioSampleID N.A.missing sample_nameID 20 22. 20141218 DDBJSequenceReadArchive 23. 20141218 DRA 30DDBJing(JST) DRA Validation BioProject BioSample 22 24. 20141218 DRA 30DDBJing(JST) ! DRA(dradev-0019) 23 25. 20141218 30DDBJing(JST) ! DRA SSH DRA DRA (dradev-0019) (fastq,bametc) http://trace.ddbj.nig.ac.jp/dra/submission.html# 24 26. 20141218 DRA1:Submission 30DDBJing(JST) ! () (Analysis) 25 27. 20141218 DRA2:Study 30DDBJing(JST) ! BioProject BioProjectID(PRJD) 26 28. 20141218 DRA3:Sample 30DDBJing(JST) ! BioSample BioSampleID(SAMD) 27 29. 20141218 DRA4:Experiment 30DDBJing(JST) ! 28 30. 20141218 DRA5:Run 30DDBJing(JST) ! RunExperiment ! Run fastqletypegeneric_fastq 29 31. 20141218 DRA 30DDBJing(JST) ! Submit 30 32. 20141218 validation 30DDBJing(JST) ! SRA validation validation 31 33. 20141218 validation 30DDBJing(JST) " (Forward100+Reverse100=200) " " " ! Experiment.SpotLength ! md5md5 ! ! 32 34. 20141218 30DDBJing(JST) ! Submission(DRA),Experiment(DRX),Run(DRR) 33 35. 20141218 BioProjectBioSampleDRA 36. 20141218 BioProjectBioSample 30DDBJing(JST) ! BioProject/BioSample ! BioProject/BioSample BioProject/BioSample DRA/DDBJ BioProject/BioSample DRA/DDBJ http://trace.ddbj.nig.ac.jp/bioproject/submission.html# 35 37. 20141218 30DDBJing(JST) ! DDBJ/EBI/NCBI DDBJDRASearch NCBIBioProject NCBIBioSample NCBISRA 36 38. 20141218 30DDBJing(JST) ! BioProject ! pubmedID BioProject BioSample DRA ! BioSample ! SamplenameID ! ! : (43) 37 39. 20141218 30DDBJing(JST) http://trace.ddbj.nig.ac.jp/contact.html ! D-way SubmissionID 38 40. 20141218 41. 2014612 30DDBJing(JST) ! Strain-leveltaxonomyIDBioSample BioProject BioSample Strain: 1 Locus tag prefix: AAAA1 BioSample Strain: 2 Locus tag prefix: AAAA2 BioSample Strain: 3 Locus tag prefix: AAAA3 BioProject Strain: 1 Locus tag prefix: AAAA1 Strain-level taxonomy ID: 10 BioProject Strain: 2 Locus tag prefix: AAAA2 Strain-level taxonomy ID: 11 BioProject Strain: 3 Locus tag prefix: AAAA3 Strain-level taxonomy ID: 12 Species-level taxonomy ID: 100 FederhenSetal.StandGenomicSci(2014)doi:10.4056/sigs.4851102 20142 20142 Genome Genome Genome Bacteria: A Strain: 1 Bacteria: A Strain: 2 Bacteria: A Strain: 3 Genome Genome Genome Bacteria: A Strain: 1 Bacteria: A Strain: 2 Bacteria: A Strain: 3 40 42. 2014612 Biological/technicalreplicates 30DDBJing(JST) ! BioSampleSRAExperiment http://trace.ddbj.nig.ac.jp/dra/faq.html#samples-for-sra 41 43. 2014612 BioProject/BioSample 30DDBJing(JST) ! DRA ! ! 42 44. 2014612 30DDBJing(JST) ! DRABioProject http://trace.ddbj.nig.ac.jp/dra/submission.html# 43 45. 2014612 md5 30DDBJing(JST) ! md5: md5: DRA md5 md5 md5 md5 md5:32 http://trace.ddbj.nig.ac.jp/dra/submission.html#__MD5_ 44