36

16

6

3a 3b

1

234

56

C250

98

50

622

36

148

64

3b3aB

1: 230LDNSDGSPSGNGA (~20-kDa)

2: 230LDNSDGSPSGNGA (~17-kDa)

3: 232NSDGSPxGNGAD (x=S; ~15-kDa)

4: 258TGNGERTADTSKGQE (~8-kDa)

5: 196VSETxPPGEGEINGNEETG (x=T)

6: 232NSDGSPSGNGAD (~19-kDa)

1 2 3 4 5 6D36

16

6

250

98

50

166

22

36

148

64

3b3aA

AAAsxThrSerGlxProGlyAlaValIleLeuLysArg

4-2-131--

1#

1--

2#4-2-131--1--

3#4-2-131-----

4#2313-31---11

5#231524-11---

6#4-2-131-----

31.7-

19.5-

8.622.68.6--

1%

7.9--

2%31.2

-19.5

-8.3

23.67.8--

9.1--

3%31.7

-19.5

-8.6

22.68.6-----

4%12.718.67.6

19.3-

21.56.2---

6.46.6

5%10.015.85.3

27.111.220.3

-4.85.0---

6%31.02.2

19.11.3

10.426.79.3-----

E F