Transcript
Page 1: 8…PFGE MLST 0×ØÙN$LF’ŁŒDOI:10.13350/j.cjpb.190315 ·!"· 8…PFGE、MLSTš MALDITOFMSÖr

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】 16735234(2019)03032305

[犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔.2019Mar;14(3):323-329.]

犛狌犫狋狔狆犻狀犵犆狉狅狀狅犫犪犮狋犲狉狑犻狋犺犘犉犌犈,犕犔犛犜,犪狀犱犕犃犔犇犐犜犗犉犕犛

LIUMing,SHIYuwen,LIUHui,LIJian,LIULanzheng,HUGuangchun (犇犻狏犻狊犻狅狀狅犳犅犪犮狋犲狉犻犪犾

犇犻狊犲犪狊犲狊,犑犻狀犪狀犕狌狀犻犮犻狆犪犾犆犲狀狋犲狉犳狅狉犇犻狊犲犪狊犲犆狅狀狋狉狅犾犪狀犱犘狉犲狏犲狀狋犻狅狀,犑犻狀犪狀,犆犺犻狀犪250021)

【犃犫狊狋狉犪犮狋】 犗犫犼犲犮狋犻狏犲 Tostudydifferentmethods(PFGE,MLST,andMALDITOFMS)ofsubtyping犆狉狅狀狅犫犪犮狋犲狉.

 犕犲狋犺狅犱狊 Eightytwoisolatesof犆狉狅狀狅犫犪犮狋犲狉weresubtypedandidentifiedwithPFGE,MLST,andMALDITOFMS.

ResultswereanalyzedusingthesoftwareBioNumericsorSaramisforclustering,andeachmethodwascomparedinterms

ofthediscriminationindex(DI). 犚犲狊狌犾狋狊 The82isolatesgenerated82massspectra(similarityrangedfrom61to

89%),81PFGEprofiles(similarityrangedfrom55.5%to100%),and68sequencetypes.Fiveclusterswereobtained

usingMALDITOFMSandMLST,andeachclustercorrespondedtoadifferentspeciesof犆狉狅狀狅犫犪犮狋犲狉.TheDIwas0.

997forPFGE,0.994forMLST,and1forMALDITOFMS. 犆狅狀犮犾狌狊犻狅狀 MALDITOFMSquicklyidentifiedanddif

ferentiated犆狉狅狀狅犫犪犮狋犲狉,butithadalowlevelofconsistency.PFGEdifferentiated犆狉狅狀狅犫犪犮狋犲狉atahigherratethan

MLST,butitwasunabletoidentify犆狉狅狀狅犫犪犮狋犲狉.MLSTaccuratelyidentifieddifferentspeciesof犆狉狅狀狅犫犪犮狋犲狉.BothPF

GEandMLSTweretimeconsuming.Thus,aMALDITOFMSdatabaseisneededtoimprovetheaccuracyofidentifica

tionof犆狉狅狀狅犫犪犮狋犲狉,andPFGEandMLSTneedtobeusedtosimultaneouslysubtype犆狉狅狀狅犫犪犮狋犲狉sothat犆狉狅狀狅犫犪犮狋犲狉

fromdifferentsourcescanberapidlyandaccuratelyidentifiedandsubtyped.

【犓犲狔狑狅狉犱狊】 犆狉狅狀狅犫犪犮狋犲狉;PFGE;MLST;MALDITOFMS

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Mar.2019, Vol.14,No.3

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Page 3: 8…PFGE MLST 0×ØÙN$LF’ŁŒDOI:10.13350/j.cjpb.190315 ·!"· 8…PFGE、MLSTš MALDITOFMSÖr

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CR0004 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(144) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5726,5756

CR0005 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(167) 犆.犱狌犫犾犻狀犲狀狊犻狊 5742,7272,9477,4738

CR0006 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(145) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,7290,5756

CR0007 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(162) 犆.犱狌犫犾犻狀犲狀狊犻狊 5741,9476,7289,8279

CR0008 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(168) 犆.犱狌犫犾犻狀犲狀狊犻狊 7271,9475,4737,9620

CR0009 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(146) 犆.狊犪犽犪狕犪犽犻犻 4481,9475,7289,5740

CR0011 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(169) 犆.犿犪犾狅狀犪狋犻犮狌狊 7289,9476,4737,7274

CR0012 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(163) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5742,5757,5708,8281

CR0013 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(148) 犆.狊犪犽犪狕犪犽犻犻 5742,9477,4738,5709

CR0014 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(17) 犆.狊犪犽犪狕犪犽犻犻 5710,5742,5726,5757

CR0015 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(150) 犆.狊犪犽犪狕犪犽犻犻 5743,5710,5757,4739

CR0016 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(7) 犆.犿犪犾狅狀犪狋犻犮狌狊 5696,5742,5725,5756

CR0019 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(151) 犆.狊犪犽犪狕犪犽犻犻 5742,9478,4738,5709

CR0022 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(42) 犆.狊犪犽犪狕犪犽犻犻 9477,8281,7289,5381

CR0023 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(164) 犆.犱狌犫犾犻狀犲狀狊犻狊 5743,7273,9478,4739

CR0024 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(17) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5726,5756

CR0025 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(152) 犆.狊犪犽犪狕犪犽犻犻 7289,9476,5380,7275

CR0026 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(159) 犆.犿犪犾狅狀犪狋犻犮狌狊 9477,7291,8281,4738

CR0027 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(172) 犆.犿犪犾狅狀犪狋犻犮狌狊 5740,9476,7289,7274

CR0028 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(173) 犆.犱狌犫犾犻狀犲狀狊犻狊 5741,5707,5756,5697

CR0030 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(165) 犆.犱狌犫犾犻狀犲狀狊犻狊 5742,5708,5697,9476

CR0031 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(166) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5742,5697,5708,5757

CR0032 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(174) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5743,9477,5708,5698

CR0033 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(175) 犆.犱狌犫犾犻狀犲狀狊犻狊 5743,5697,5708,5757

CR0034 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(176) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5742,5708,9478,4738

CR0035 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(4) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5756,9478

CR0037 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(143) 犆.狊犪犽犪狕犪犽犻犻 5741,5709,5755,9477

CR0039 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狋狌狉犻犮犲狀狊犻狊(153) 犆.狋狌狉犻犮犲狀狊犻狊 5856,4738,5709,5697

CR0040 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(177) 犆.犱狌犫犾犻狀犲狀狊犻狊 5742,5708,5697,9478

CR0041 � 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(178) 犆.狊犪犽犪狕犪犽犻犻 5708,5741,5724,5754

CR0042 � 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(179) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5743,5708,5670,5693

CR0044 è_ 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(154) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5731,5756

CR0045 è_ 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(40) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5755,7289

CR0046 è_ 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(161) 犆.狊犪犽犪狕犪犽犻犻 5709,5741,5724,9477

CR0048 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(155) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5725,8132

CR0054 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(40) 犆.狊犪犽犪狕犪犽犻犻 5708,5741,5725,5755

CR0055 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(224) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5756,5726

CR0058 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狋狌狉犻犮犲狀狊犻狊(463) 犆.狊犪犽犪狕犪犽犻犻/犆.狋狌狉犻犮犲狀狊犻狊 5857,5698,5710,9477

CR0059 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(464) 犆.犿狌狔狋犼犲狀狊犻犻 5713,8100,5700,9477

CR0060 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(308) 犆.狊犪犽犪狕犪犽犻犻 5708,5741,5725,5755

CR0061 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(458) 犆.狊犪犽犪狕犪犽犻犻 5708,5741,5755,8131

CR0062 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(146) 犆.狊犪犽犪狕犪犽犻犻 5709,5741,5725,4482

CR0063 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(487) 犆.犱狌犫犾犻狀犲狀狊犻狊/犆.狊犪犽犪狕犪犽犻犻 5742,5708,5697,5742

CR0065 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(12) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5725,5756

CR0066 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(46) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5726,5756

CR0067 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(4) 犆.狊犪犽犪狕犪犽犻犻 5742,5710,9478,4739

CR0068 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(465) 犆.犿犪犾狅狀犪狋犻犮狌狊 9478,5741,4738,7275

CR0069 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犱狌犫犾犻狀犲狀狊犻狊(517) 犆.犱狌犫犾犻狀犲狀狊犻狊 5741,7270,9476,5707

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Mar.2019, Vol.14,No.3

Page 4: 8…PFGE MLST 0×ØÙN$LF’ŁŒDOI:10.13350/j.cjpb.190315 ·!"· 8…PFGE、MLSTš MALDITOFMSÖr

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CR0070 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(22) 犆.狊犪犽犪狕犪犽犻犻 5707,5740,5754,9475

CR0071 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狋狌狉犻犮犲狀狊犻狊(32) 犆.狋狌狉犻犮犲狀狊犻狊 5856,5696,5708,5722

CR0072 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(148) 犆.狊犪犽犪狕犪犽犻犻 5741,5709,5756,9477

CR0073 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(4) 犆.狊犪犽犪狕犪犽犻犻 5742,4738,9478,5709

CR0074 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狋狌狉犻犮犲狀狊犻狊(466) 犆.狊犪犽犪狕犪犽犻犻/犆.狋狌狉犻犮犲狀狊犻狊 5698,5857,5710,8085

CR0075 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(488) 犆.狊犪犽犪狕犪犽犻犻 5709,5741,9477,5724

CR0076 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(483) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5763,4738

CR0077 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(484) 犆.狊犪犽犪狕犪犽犻犻 5741,5709,5755,9476

CR0079 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(64) 犆.狊犪犽犪狕犪犽犻犻 5709,5741,5731,5756

CR0080 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(12) 犆.狊犪犽犪狕犪犽犻犻 5742,5710,5757,9478

CR0081 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(13) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5725,5756

CR0082 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(489) 犆.犿狌狔狋犼犲狀狊犻犻 5712,5698,5726,4625

CR0085 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(148) 犆.狊犪犽犪狕犪犽犻犻 9478,4738,7290,5381

CR0086 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(3) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5756,7290

CR0087 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(485) 犆.狊犪犽犪狕犪犽犻犻 9479,4739,7291,5742

CR0088 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(1) 犆.狊犪犽犪狕犪犽犻犻 9476,9558,4737,5380

CR0091 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(23) 犆.狊犪犽犪狕犪犽犻犻 9477,7289,5380,7276

CR0092 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(493) 犆.犿狌狔狋犼犲狀狊犻犻 5711,5726,7348,4697

CR0093 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(494) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5756,5726

CR0094 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(494) 犆.狊犪犽犪狕犪犽犻犻 5709,5742,5726,9478

CR0095 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(486) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,4482,9480

CR0096 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(526) 犆.狊犪犽犪狕犪犽犻犻 5742,5693,5707,5756

CR0097 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(64) 犆.狊犪犽犪狕犪犽犻犻 5709,5741,5725,5756

CR0098 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(60) 犆.犿犪犾狅狀犪狋犻犮狌狊 5741,7290,5709,9477

CR0099 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(64) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5756,5671

CR0100 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(4) 犆.狊犪犽犪狕犪犽犻犻 5742,5709,5726,5756

CR0101 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(527) 犆.狊犪犽犪狕犪犽犻犻 5742,5710,5726,5757

CR0102 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(528) 犆.犿犪犾狅狀犪狋犻犮狌狊 5741,5709,5756,5698

CR0103 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(495) 犆.狊犪犽犪狕犪犽犻犻 5743,5710,5757,9478

CR0104 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.狊犪犽犪狕犪犽犻犻(21) 犆.狊犪犽犪狕犪犽犻犻 5744,5711,5758,5727

CR0105 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(529) 犆.犿犪犾狅狀犪狋犻犮狌狊 5742,5709,5756,5726

CR0106 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(530) 犆.犿狌狔狋犼犲狀狊犻犻 5713,5381,7349,9477

CR0107 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(531) 犆.犿狌狔狋犼犲狀狊犻犻 5711,5697,5725,4696

CR0108 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿狌狔狋犼犲狀狊犻犻(532) 犆.犿狌狔狋犼犲狀狊犻犻 5712,5733,7348,5627

CR0109 �x 犆.狊犪犽犪狕犪犽犻犻group 犆.犿犪犾狅狀犪狋犻犮狌狊(7) 犆.犿犪犾狅狀犪狋犻犮狌狊 7317,9475,4737,5740

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Mar.2019, Vol.14,No.3


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