Download - ALAT-ALAT PCR & Elektroforesis 2011
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ALAT-ALAT PCR (Polymerase Chain Reaction), ALAT
ELEKTROFORESIS DNA DAN DNA SQUENCING
OLEH : MARUNI WIWIN DIARTI,S.SI,M.KES
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Polymerase Chain Reaction(PCR)
Suatu metode enzimatik untuk mengamplifikasi (=menggandakan) fragmen DNA secara spesifik.
Bekerja berdasarkan pada sistem pemanasan dan pendinginan berulang, terkontrol dan otomatis dalam suatu perangkat/mesin, yaitu thermal cycler.
Dapat bekerja secara sensitif dan spesifik.
Aplikasi untuk diagnostik dan penelitian.
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ALAT-ALAT PCR
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DNA THERMAL CYCLER (MESIN PCR)
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DNA THERMAL CYCLER (MESIN PCR)
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DNA THERMAL CYCLER (MESIN PCR)
• KEGUNAAN:OTOMATISASI REAKSI
• CONTOH:THERMOLYNE AMPLITRON IMYCYCLER BIORAD
• TAHAPAN REAKSI:- DENATURASI- ANNEALING- POLIMERISASI/ELONGATION
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MICROCENTRIFUGE
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MICROCENTRIFUGE
• KEGUNAAN:PENGENDAPAN DNA
• CONTOH:TOMY MC-140
• KECEPATAN:14.000 rpm.
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WATERBATH & VORTEX MIXER (MENCAMPUR PEREAKSI/BARNSTEAD)
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MICROPIPETTE
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MICROPIPETTE
• KEGUNAAN:MENCAMPUR PEREAKSI/MENGAMBIL PEREAKSI ATAU SAMPEL
• CONTOH:EPPENDORF
• VOLUME:0,5 – 10 µl
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FREEZER -20OC
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FREEZER -20OC
• KEGUNAAN:MENYIMPAN REAGEN
• CONTOH:MERK DERBY, SANYO
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DEEP FREEZER -80OC
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DEEP FREEZER -80OC
• KEGUNAAN:MENYIMPAN DNA, RNA
• CONTOH:SANYO (MDF-U281)
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ELEKTROPHORESIS DNA
• Elektroforesis suatu tehnik pemisahan makro molekul (seperti protein dan asam nukleat) pemisahan berdaarkan BM
• Elektroforesis = kromatografi. • Prinsip dasar bekerjanya elektroforesis ialah
adanya perbedaan kecepatan gerak partikel-partikel bermuatan bila berada dalam suatu medan listrik
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ELEKTROPHORESIS DNA
• Pada elektroforesis ini bahan yang akan dipisahkan ditempatkan dalam tabung bentuk U yang kedua ujungnya dihubungkan dengan anoda dan katoda dan berisi larutan dapar sebagai pelarutnya.
• Apabila kepada sistem dipasang tegangan searah, ion-ion dalam larutan dapar akan bergerak dengan kecepatan yang tergantung kepada, antara lain: muatan netto, ukuran bahan dan bentuk ionnya.
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ELEKTROPHORESIS DNA
• SUB-MARINE GEL ELEKTROPHORESIS app.ANALISA PRODUK PCR BIORAD (MINI SUB CELL)
• ELECTROPHORESIS POWER SUPPLY DNA ELECTROPHORESIS BIORAD PAC 1000
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04/17/23 Yunan Jiwintarum/2008
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04/17/23 Yunan Jiwintarum/2008
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04/17/23 Yunan Jiwintarum/2008
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UV TRANSILUMINATOR
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KEGUNAAN: ANALISA PRODUK PCR
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04/17/23 Yunan Jiwintarum/2008
pcr
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ELEKTROFORESIS PROTEIN
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ALAT ELEKTROFORESIS PROTEIN
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HASIL ELEKTROFORESIS PROTEIN BERUPA BAND ATAU PITA
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BAHAN DAN REAGEN UNTUK ELEKTROFORESIS
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