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BAKTERIOLOGI INFEKSI KULIT & JARINGAN LUNAK
DICKY BAGUS WIDHYATMOKO
18 – 19 MARET 2015DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERANUNIVERSITAS AIRLANGGA
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POKOK BAHASAN
Staphylococcus spp
Mycobacterium leprae
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DISEDIAKAN
Staphylococcus :
S. aureus
S. epidermidis
S. saphrophyticus
Mycobacterium leprae :
slide only
Uji Katalase
Uji Koagulase
Mannitol Salt Agar (MSA)
Novobiocin test
MRSA Identification
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Identifikasi Staphylococcus
Uji Katalase
Uji Koagulase
Mannitol Salt Agar (MSA)
Novobiocin test
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Staphylococcus spp
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Use of Catalase Test
The catalase test is primarily used todistinguish among Gram-positive cocci
Staphylococcus are catalase-positive,
members of the genera Streptococcus andEnterococcus are catalase-negative.
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Catalase Test
Metode :
slide (drop) method
tube method
tube (slant) method
Catalase2H2O2 O2 + 2H2O
Streptococci vs. Staphylococci
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Catalase Test
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Catalase POS
Staphylococcus
Catalase NEG
Catalase Test
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Catalase Test (tube method)
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Catalase Test (tube (slant) method)
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Coagulase Test
differentiates strains of Staphylococcus aureusfrom S. epidermidis and other coagulase-negative species
S. aureus strains are usually capable ofcoagulating EDTA-treated plasma in the tubetest and will produce clumps of cells in theslide test.
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Coagulase Test
method :
Slide Test
Tube Test
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Coagulase Test (Slide Test)
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S. aureus
Coagulase
Fibrinogen Fibrin
Differential Characteristics
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Coagulase NEG
Coagulase POS
Staphylococcus aureus
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Mannitol Salt Agar
Contains :
Mannitol
Phenol Red as a pH indicator (pH = 7.8)
Reaction
Mannitol Fermented acid produced
pH < 6.9 : yellow color
pH > 8.4 : red color
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various spesies on Tripticase Soy Agar (TSA)
A. S. aureus B. S. epidermidis C. E. coli
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various spesies on Tripticase Soy Agar (TSA)
A. S. aureus B. S. epidermidis C. E. coli
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Novobiocin Test
Differentiate between S. epidermidis w/ S. saprophyticus
Using Novobiocin disc ( 5 g)
< 15 mm = Resisten S. saprophyticus
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Identifikasi MRSA
MRSA : Methicillin-Resistant Staphylococcus aureus
Methicillin Oxacillin Cefoxitin
CLSI M100
Using Cefoxitin disc :
21 mm : MRSA
> 21 mm : non - MRSA
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Suspect MRSA
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Collect Sample from wound
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Collect Sample from nose
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Mycobacterium Leprae
Sample : Skin scrap using scalpel
Using Slide with Ziehl-Neelsen Stain method
Presence of globi (singular = globus):encapsulated amphophilic masses of non-viable bacilli found within macrophages or inextracellular space
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Result : (Bacterial Index)
1+ At least 1 bacillus in every 100 fields.
2+ At least 1 bacillus in every 10 fields.
3+ At least 1 bacillus in every field.
4+ At least 10 bacilli in every field.
5+ At least 100 bacilli in every field.
6+ At least 1000 bacilli in every field.
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On Hand
Pengamatan & Pelaporan Preparat Demo
Uji Katalase
Kultur Media dari spesimen langsung (amies swab) dengan :
Blood Agar Plate
McConkey Agar
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