Download - biomol-Mikroskop Elektron
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Mikroskop Elektron
Sutiman B. SumitroGuru Besar Biologi Sel
Universitas Brawijaya
Understanding Size
1 metre
Understanding Size
10 centimetres
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Understanding Size
1 centimetre
Understanding Size
100 micrometres
Understanding Size
10 micrometres
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Understanding Size1 micrometre
Understanding Size100 nanometres
Understanding Size
10 nanometres
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Understanding Size1 nanometre
Tahun 1973 Tahun 2013
Mikroskop Elektron Transmisi
The electron microscope uses electrostatic and
electromagnetic lenses
Electron microscopes are used to investigate the ultrastructure of
a wide range of biological and inorganic specimens
Industrially, the electron microscope is often used for quality
control and failure analysis.
Modern electron microscopes produce electron micrographs,
using specialized digital cameras or frame grabbers to capture
the image
Resolustion achived to 50 piko meter (pm) resolutionandmagnifications of up to about 10,000,000x
https://en.wikipedia.org/wiki/Electrostatic_lenshttps://en.wikipedia.org/wiki/Electromagnetismhttps://en.wikipedia.org/wiki/Ultrastructurehttps://en.wikipedia.org/wiki/Micrographhttps://en.wikipedia.org/wiki/Frame_grabberhttps://en.wikipedia.org/wiki/Frame_grabberhttps://en.wikipedia.org/wiki/Micrographhttps://en.wikipedia.org/wiki/Ultrastructurehttps://en.wikipedia.org/wiki/Electromagnetismhttps://en.wikipedia.org/wiki/Electrostatic_lens
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Resolusi (Daya Pisah)
1 nm 100 nm
Newtonian
Gravitation,
speed
Space and time
Measurable
dimension,
weigh, andspeed
Modern
Physics
Quantum
Physics
Atom andSubatomic,
particless
Time and
space less
Nano Science
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Sample preparation
A sample of cells (black) stained withosmium tetroxide and uranyl acetateembedded in epoxy resin (amber) ready for
sectioning.
Tissue Sectioning
A diamond knife blade used for cutting
ultrathin sections (typically 70 to 350 nmfor transmission electron microscopy.
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Imaging methods
Contrast formation
Contrast formation in the TEM depends greatly on the mode of operation.Complex imaging techniques, which utilize the unique ability to change lens
strength or to deactivate a lens, allow for many operating modes
Bright field The most common mode o f operation for a TEM is the bright
field imaging mode
Diffraction contrast known as a dark-field image.
Electron energy loss This normally results in chromatic aberration –
however this effect can, fo r example, be used to generate an image which
provides information on elemental composition, based upon the atomic
transition during electron-electron interaction.
Phase contrast Crystal structure can also be investigated by High-
Resolution Transmission Electron Microscopy (HRTEM)
Diffraction This image provides the investigator with information about thespace group symmetries in the crystal and the crystal's orientation
TEM can be modified into a
Scanning TransmissionElectron Microscope (STEM)
by the addition of a system
that rasters the beam acrossthe sample to form the image
Scaning
Transmision
Electon
Microscope
STEM)
A three-dimensiona l T EM image of a parapoxavirus
http://en.wikipedia.org/wiki/Space_grouphttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://localhost/var/www/apps/conversion/tmp/scratch_7/TEM-parapoxvirus-tomograph.ogv.480p.webmhttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Space_group
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Scaning Electron Microscope
M. von Ardenne's f irst SEM
Images of Scanning Electron
Microscope
1 micrometre
10 micrometres
100 nanometres
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SEM
NORMAL
OSTEOPOROSIS
XRD PATTERN
XRD PATTERN
http://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdf
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Average of crystal size in osteoporosis
0
50
100
150
200
( n m )
HA-Synthetic Normal Osteoporosis
Skull Bone Cortical Bone
Crystal Size
Data (15) D (15)
Crystal size
(nm)
1 HAp std 1899,15 189,92
2 S-16 263,31 26,33
3 S-15 197,43 19,74
4 S-2 263,85 26,39
5 S-4 716,83 71,68
6 M5 790,46 79,05
7 Bone Skull 88,99 8,90
8 Normal
Cortical Bone
350,73 35,07
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Atomic distribution pattern in osteoporosis
Ca P
Cu Zn
Al Fe K Mg S Ti
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Atomic distribution pattern in normal
Ca K Ti
Cu Zn Fe
Al P Mg S
Comparation of mapping atom (SEM-EDAX)
Pattern Osteoporosis Normal Periodic
1 Ca, P Ca, K, Ti IV
2 Cu, Zn Cu, Zn, Fe IV
3 Al, Fe, K, Mg, S, Ti Al, P, Mg, S III
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http://localhost/var/www/apps/conversion/Periodic%20Table.lnkhttp://localhost/var/www/apps/conversion/Periodic%20Table.lnk