Download - Cytology of Body Fluid
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Cytology of Body FluidSpeaker : Advisor : Date : 3.15.2006Edmund S, Cytology, Chapter 4: Pleural, pericardial, and peritoneal fluid Richard M DeMay, The art & science of cytopathology, Chapter 8: FluidLeopold G.Koss, Koss diagnostic cytology, Chapter 26: Effusion in the presence of cancer
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OutlineRepresentation of the three body cavitiesCollection and preparation of specimen Benign elementsNon-neoplastic conditionsMalignant effusions---primary tumors ---metastatic tumorsDifferences Between Adenocarcinoma and Mesothelioma
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Schematic representation of the three body cavities
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Accumulation of fluids in body cavities
Differences Between a Transudate and an ExudateFeature Transudate ExudateGross appearance Watery, clear Cloudy, reddishSpecific gravity 1.015Protein 3.0 g/dlClots No YesCells Few; usually benign Many; can be malignant
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Collection and preparation of specimen
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Benign elementsMesothelial cells
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Non-neoplastic conditionsEosinophilic pleural fluid
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Tuberculous pleuritisMultinucleated macrophages
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Systemic lupus erythematosus Cytology preparation: Lupus erythematosus cell (LE cell)LE cell
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Malignant effusions---primary tumors
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More and bigger cells, in more and bigger clustersCell-in-cell pattern
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Malignant effusions---metastatic tumors
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F4.27F4.28
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Non-Hodginkin lymphomaLarge cell lymphoma Nuclei large than histiocyte Eccentric nuclei Abundant blue cytoplasm Best appreciated in Diff-QuikFollicular lymphoma Irregular nuclear contours Scant cytoplasm
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lymphoblastic lymphoma Small to medium sized lymphocytes Fine powdery chromatin Scant cytoplasm
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Differences Between Adenocarcinoma and Mesothelioma
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C: cytoplasm; M: membrane; N: nuclear +++++++++++--------++---
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E-cadherin
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BerEP4
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B72.3
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Calretinin