Discover the UniProt Blast tool
Murcia, February, 2011Protein Sequence Databases
Customize the BLAST results
Look for the local alignment
Highlight important positions within the alignment according to the sequence annotation
The metal binding sites are conserved
HBB_HUMAN
Discover the UniProt Align tool
1- Select sequences of interest… (from Blast results for example)
2 - Align…
Add the ‘query’ sequence(HBB_HUMAN)
Conservation of the metal-binding site….
Highlight important positions within the alignment according to the sequence annotation
Compare the Blast
@ UniProt and
@ NCBI
Protein Sequence Databases
The ‘alternative’ sequence(s)
not ‘directly available’ for a lot of tools, including protein identification tools, Blast, depending on the server !….
Murcia, February, 2011
Blast P04150 against Swiss-Prot / homo sapiens @ UniProt
Isoform sequences
Murcia, February, 2011Protein Sequence Databases
Protein Sequence Databases
Blast P04150 against Swiss-Prot / homo sapiens @ NCBI
The isoform sequences (from Swiss-Prot) are not present in the NCBI protein database !The .x number (P06401.4) correspond to the version number of the sequence…not to an alternatively spliced sequence !
Murcia, February, 2011