![Page 1: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/1.jpg)
Gram Stain of a Clinical Specimen of Candida albicans
Gram Stain of a Clinical Specimen of Candida albicans
Courtesy of University of Washington; with permission.
![Page 2: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/2.jpg)
Growth on Sabouraud's Dextrose Media
![Page 3: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/3.jpg)
الشريحه في الصورة عن تتحدثالتاليه
• Gram Stain• Gram + stain of cocci arranged in culster
![Page 4: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/4.jpg)
Staphylococcus
![Page 5: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/5.jpg)
Staphylococci Stained in Pus
![Page 6: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/6.jpg)
Gram-Positive Streptococcus
![Page 7: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/7.jpg)
Sputum stained with gram positive
microorganism
pus
![Page 8: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/8.jpg)
StreptococcusStaphylococcus
![Page 9: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/9.jpg)
Streptococcus pyogenes = Group A Strep
Carried by many peoplein throat or on skin
often no symptoms
Cause of • strep throat• Impetigo الجلد اصابه• Necrotizing االنسجه موت fasciitis
القاموس منA bacterium that
causes the formation of pus or of fatal septicemias
![Page 10: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/10.jpg)
Streptococcus pyogenes. Left. Gram stain of Streptococcus pyogenes in a clinical specimen. Right. Colonies of Streptococcus pyogenes on blood agar exhibiting beta (clear) hemolysis
![Page 11: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/11.jpg)
Beta hemolysis
![Page 12: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/12.jpg)
Alpha-hemolysis
• Alpha-hemolytic Streptococcus species "Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis.
احفظ االنواع
المسببه مهمه
![Page 13: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/13.jpg)
Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis. (Also see Figures 15,16 and 23-25).
Alpha-hemolytic Streptococcus species
![Page 14: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/14.jpg)
![Page 15: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/15.jpg)
Bacitracin مضاد اسمsensitivity
Demonstrations and practial
• Principle:– Bacitracin test is used for presumptive
identification of group A للترجيح– To distinguish between S. pyogenes
(susceptible حساس to Bacitracin) & non group A such as S. agalactiae (Resistant to B)
– Bacitracin will inhibit the growth of gp A Strep. pyogenes giving zone of inhibition around the disk
• Procedure:– Inoculate يلقح BAP ( فيها اللي الميديا االجار
with heavy suspension of tested المضاد (organism
– Bacitracin disk (0.04 U) is applied to inoculated BAP الملقحه العينه
– After incubation الحضانه any zone of ,فترهinhibition around the disk is considered as susceptible يعني المضاد هذا من حساسأي) ( قروب
الدائره هي
العالمه الفارقه
Group A
![Page 16: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/16.jpg)
Optochin Susceptibility Test
Optochin resistantS. viridans
Optochin resistantS. viridans
Optochin susceptibleS. pneumoniae
اللي االختبار فكره نفس اختبار راح
هي هنا الحساسه البكتيريا : بسs.pneumonia
![Page 17: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/17.jpg)
Lowenstein-Jensen medium
Lowenstein-Jensen medium
Mycobacterium tuberculosis
Mycobacterium tuberculosis
Name of its medium
![Page 18: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/18.jpg)
Mycobacterium tuberculosis Ziehl-Neelsen stain
![Page 19: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/19.jpg)
Acid-fast bacilli=redish colour
![Page 20: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/20.jpg)
Aspergillus niger
Culture of Aspergillus niger. Conidial head of A. niger
![Page 22: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/22.jpg)
alpha hemolysis (green zone surrounding colonies). Note the zone of inhibition around a filter paper disc impregnated عليه with optochin. Viridans وضعstreptococci are not inhibited by optochin.
Streptococcus pneumoniae - optochin sensitivity test
![Page 23: Gram Stain of a Clinical Specimen of Candida albicans Courtesy of University of Washington; with permission](https://reader035.vdocuments.pub/reader035/viewer/2022062519/5697bfe11a28abf838cb4082/html5/thumbnails/23.jpg)
Haemophilus Species
Direct smear لطاخه of H. influenzae in CSF in a case of meningitis. Note the intracellular and extracellular pleomorphic االشكال -gram متعددnegative bacilli.