Gram Stain of a Clinical Specimen of Candida albicans
Gram Stain of a Clinical Specimen of Candida albicans
Courtesy of University of Washington; with permission.
Growth on Sabouraud's Dextrose Media
الشريحه في الصو�رة عن تتحدثالتاليه
• Gram Stain• Gram + stain of cocci arranged in culster
Staphylococcus
Staphylococci Stained in Pus
Gram-Positive Streptococcus
Sputum stained with gram positive
microorganismpus
StreptococcusStaphylococcus
Streptococcus pyogenes = Group A Strep
Carried by many peoplein throat or on skin
often no symptoms
Cause of • strep throat• Impetigo الجلد اصابه• Necrotizing االنسجه موت fasciitis
القاموس منA bacterium that
causes the formation of pus or of fatal septicemias
Streptococcus pyogenes. Left. Gram stain of Streptococcus pyogenes in a clinical specimen. Right. Colonies of Streptococcus pyogenes on blood agar exhibiting beta (clear) hemolysis
Beta hemolysis
Alpha-hemolysis
• Alpha-hemolytic Streptococcus species "Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis.
احفظ االنواع
المسببه مهمه
Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis. (Also see Figures 15,16 and 23-25).
Alpha-hemolytic Streptococcus species
Bacitracin مضاد اسمsensitivity
Demonstrations and practial
• Principle:– Bacitracin test is used for presumptive
identification of group A للترجيح– To distinguish between S. pyogenes
(susceptible حساس to Bacitracin) & non group A such as S. agalactiae (Resistant to B)
– Bacitracin will inhibit the growth of gp A Strep. pyogenes giving zone of inhibition around the disk
• Procedure:– Inoculate يلقح BAP ( فيها اللي الميديا االجار
with heavy suspension of tested المضاد (organism
– Bacitracin disk (0.04 U) is applied to inoculated BAP الملقحه العينه
– After incubation الحضانه any zone of ,فترهinhibition around the disk is considered as susceptible يعني المضاد هذا من حساسأي) ( قروب
الدائره هي
العالمه الفارقه
Group A
Optochin Susceptibility Test
Optochin resistantS. viridans
Optochin resistantS. viridans
Optochin susceptibleS. pneumoniae
اللي االختبار فكره نفس اختبار راح
هي هنا الحساسه البكتيريا : بسs.pneumonia
Lowenstein-Jensen medium
Lowenstein-Jensen medium
Mycobacterium tuberculosis
Mycobacterium tuberculosis
Name of its medium
Mycobacterium tuberculosis Ziehl-Neelsen stain
Acid-fast bacilli=redish colour
Aspergillus niger
Culture of Aspergillus niger. Conidial head of A. niger
alpha hemolysis (green zone surrounding colonies). Note the zone of inhibition around a filter paper disc impregnated عليه with optochin. Viridans وضعstreptococci are not inhibited by optochin.
Streptococcus pneumoniae - optochin sensitivity test
Haemophilus Species
Direct smear لطاخه of H. influenzae in CSF in a case of meningitis. Note the intracellular and extracellular pleomorphic االشكال -gram متعددnegative bacilli.