Download - Hemopoietic growth factors
Hemopoietic Growth Factors: A Review George Morstyn and Antony W. Burgess1
Melbourne Tumour Biology Branch, Ludwig Institute for Cancer Research,
Australia 3050 CANCER RESEARCH 48. 5624-5637, October 15. 1988
อาจารยท่ี์ปรกึษา : อาจารย ์ประสทิธิ ์ชนะรตัน์
Section 132 group 3
Hemopoietic system
Type of Colony stimulating factor
(CSF) Granulocyte-Macrophage Colony
Stimulating Factor (GM-CSF) Granulocyte Colony stimulating factor
(G-CSF) Macrophage Colony stimulating factor
(M-CSF) Multi Colony stimulating factor (multi-
CSF)
Colony stimulating factor Synthesis
Bone marrow + Cell line
Recombinant Natural cDNA of CSF Cell stimulation CFS Bacteria Stem cell
CSF Colonies Colony Testing
Granulocyte-Macrophage
Colony Stimulating Factor (GM-CSF)
Stimulated Granulocyte and Macrophage series in murine and human
Granulocyte Colony stimulating factor
(G-CSF) Human G -CSFSynthesized from recombinant DNA methodCan not diferented between natural G-CSF and recombinant G-CSF
Homology between human and murine G-CSF approximately 75 %
Differentiation of biological and species
Macrophage Colony stimulating factor
(M-CSF) Also called CSF-1 Active only in dimer form Human urinary M-CSF
stimulate for the final stages of granulocytic development in humans
stimulator of the early monocytic progenitor cells in mouse marrow
Murine M-CSFStimulate mouse marrow cell Not stimulate human marrow cell
Multi-Colony Stimulating Factor
(multi-CSF) Also called IL-3 Stimulate granulocyte ,macrophage and erythroid cell
Effects of Colony Stimulating Factors
on Mature Cells
In Vivo Effects of CSFs
Granulocyte-Macrophage Colony Stimulating FactorMurine studies increase in the peritoneum of neutrophils,monocytes, and eosinophils decrease in lymphocytes.Simian studies not restricted to the myeloid system
Multi-Colony Stimulating Factor
effects of multi-CSF in mice
peritoneal cavity increased of monocytes and these monocytes appeared to functionas more effective phagocytes.
Granulocyte Colony Stimulating Factor.
Hemopoietic Growth Factors and Leukemia CSFs suppression of normal
hemopoiesis by the leukemic cell of patients with acute myeloid leukemia ( AML)
Production and Storage of GM-CSF Most organs can produce the granulocyte
macrophage colony stimulating factors bioassays salivary gland, lung, thymus, kidney, spleen, lymph
node, pancreas, brain,heart, small intestine, testis, skeletal muscle, liver, and skin.
Endotoxin and tumor necrosis factor appear to stimulate CSF release
GM-CSF production by endothelial cells, smooth muscle, and fibroblasts and that these can be stimulated to produce CSF by tumor necrosis factor
GM-CSF is to identify the cells and tissues where GM-CSF mRNA is present
Clinical Disorders and Abnormal GM-
CSF Levels CSFs can control hemopoiesis in vivo direct and indirect
Correlation between urine CSF levels and WBC levels
Detected human G-CSF but not human GM-CSF
Early Clinical Trials with G-CSF
Future Directions
Clinical situations ; ovarian cancer ,lung cancerhigh concentrations GM-CSF inhibits the proliferation of small cell lung cancer cells.
Potential Side Effects of CSF
Therapy The direct effects of the colony stimulating factors will be confined to cells that contain CSF receptors
the stimulated cells may be induced to secrete other biological response modifiers which could in fluence the action of these molecules.
Another: potential side effect is the development of antibodies to CSF.
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