Download - Macromolecular Nanomachines In Action
IN ACTION
José Ramón Macías
macromolecularnanomachines
José Ramón Macías
Hi, my name is:
Bioinformatician
macromolecularnanomachines
IN ACTION
Structure
Sequence
Structure
Function
Sequence
10 nm
1 mm
100 nm
X-Ray DiffractionCrystallography
conformational changes
Simian Virus 40
Large T antigen
Simian virus 40(SV40)
Large T antigen
Short & straight
Long & straight
Middle curvature
High curvature
Short & straight
Long & straight
Middle curvature
High curvature
Bacteriophage SPP1 replicative
Helicase G40P
D. melanogaster
26S proteasome
26S proteasome
X-Ray Crystallography
Requires Crystals
High Resolution
X-Ray Crystallography
Requires Crystals
High Resolution
3D Electron Microscopy
Medium Resolution
Crystals Not required
X-Ray Crystallography
Requires Crystals
High Resolution
3D Electron Microscopy
Medium Resolution
Crystals Not required
X-Ray Crystallography
Requires Crystals
High Resolution
3D Electron Microscopy
Medium Resolution
Crystals Not required
X-Ray Crystallography
Requires Crystals
High Resolution
3D Electron Microscopy
Medium Resolution
Crystals Not required
Hybrid Models
X-Ray Crystallography
Requires Crystals
High Resolution
3D Electron Microscopy
Medium Resolution
Crystals Not required
Hybrid Models
Fitting
3D-EM model Atomic model
Hybrid model
GroEL chaperonin
Anti-tumoralP53 transcription factor
lectron
icroscopy
ata
ank
E
M
D
B
10
40
67
117
193
266
363
2002 2003 2004 2005 2006 2007 2008
since June 2002, the number of structures has increasedsteadily.
“
”
Single Particle Icosahedral
HelicalIndividual Structure 2D Crystal
Today there are629 entries
1 out of 3 structures
are covered by more than one entry
369
http://biocomp.cnb.csic.es/das/PeppeR
DASx3DEM & PeppeR
Structure
Sequence
adopting different conformations
is what leads to function
