DNA REPLICATION
Nucleotid & Nucleosid
Nucleosid
*Figure 07.08
*Figure 07.09
*Figure 07.18
Polymerization of the deoxynucleotides during DNA synthesis. The β and γ phosphates of each deoxynuleosid triphosphate are cleaved off to give energy for the polymerization reaction
A single base can be flipped out from the double helix,which could be important in recombination and repair
*Figure 01.11
*Figure 09.23
*DNA polymerase { ΙΙΙ , Ι }
*Nuclease { endonuclease , exonuclease [ 5´,3´ exonuclease]}
*DNA ligase
*Primase
*Accessory proteins : 10 different protein: β clamp → dnaN gene , clamp loader → help the β clamp
*Helicases and helix- desabilizing proteins or single strand DNA binding proteins
*Topoisomerases
*Figure 09.18
ori C
Initiation of replication at E.coli oriC
DNA Repair*Mismatch repair
*Excision repair
*Recombination repair
*SOS repair
Mismatch repair(methyl-directed mismatch repair)