ori pVS1pTF101.1

9.2 kbp

TEV enhancer

RB

Tvsp

LB

ori colE1

2x P35S

aadA

bar

ori pSa(incW)pTF101.1::UCD2

13.0 kbp

tetr

ori RK2(incP)

pTF101.1::BIN19

11.0 kbpkanr

ori pRipTF101.1::Ri

10.1 kbp

ori RK2(incP)pE2759

30.1 kbp

tetr

pgl

picASupplemental Figure 1. Schematic representation of the various T-DNA binary vectors and integration plasmid used in this study. The parent plasmid was pTF101.1. The region highlighted in blue indicates the T-DNA region plus the aadA gene incorporated into all other plasmids. LB/RB, T-DNA

left/right border regions; ori, vegetative origin of replication; 2xP35S, cauliflower mosaic virus double 35S promoter; TEV, tobacco etch virus; bar, phoshinothricin-resistance gene; Tvsp, vsp polyA addition signal; aadA, spectinomycin-resistance gene; tet/kanr, tetracycline/kanamycin resistance gene; pgl/picA, pgl/picA genes from the C58 chromosome.