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Single cell assay on CD-like lab chip using centrifugal
single cell trap
Microsystem Research Center, Korea Institute of Science and Technology
報告人:微機電所陳治忠
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Types of signaling
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Conventional method to isolate single cell
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Chip design consisting of chamber channel and valve, and trap
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Experimental setup
Control module CCD Motor parts of for rotating chip
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Microfluidic component on CD-chip
Photo image of microfluidic pattern Schematic sideview of CD-chip
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Cell trap assay
Cell trap VS duration time Cell trap VS trap size
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Survival rate of cells with the treatment of PFA
Single cell assay at CD-chip MTT assay of crowed cells at 96 well plates
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Rate of apoptotic cells with the expose of UV
Fluorescence microscope image of dead cellsFluorescence intensity data was cutoff at 10% of average value
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Conclusion
• The ratio of trapping cells were almost 80% and more than 600 cells can be test in a experiment
• The trapping efficiency was main effected by the size of trapping hole
• Cytotoxicity assay showed the single cell might not be affected by the neighboring cells
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• Fluorescein isothiocyanate (FITC)