Sheet13120430303030AllozymeRVARVBLKALKBCODESITEESTFBAMDHD0101RVA100981009010096D0102RVA8086901008686D0103RVA909280909298D0104RVA9810084809880D0105RVA100981008486100D0106RVA8210010010092100D0107RVA949810010010090D0108RVA1001009410010098D0109RVA8890948610082D0110RVA90801009298100D0111RVA100849010094100D0112RVA90100801008496D0113RVA801008410010086D0114RVA841001009810098D0115RVA100941009410080D0116RVA100941008490100D0117RVA10010084100100100D0118RVA9492981009090D0119RVA949486928092D0120RVA10090921008498D0121RVA1009210010010086D0122RVA9210010010010092D0123RVA94100100100100100D0124RVA90100981009292D0125RVA921009494100100D0126RVA1009684100100100D0127RVA10090100100100100D0128RVA1008010092100100D0129RVA1009210094100100D0130RVA961009290100100D0131RVB9010010092100100D0132RVB801001001009292D0133RVB92961001009494D0134RVB100901001009090D0135RVB100801001009292D0136RVB100928496100100D0137RVB1001009490100100D0138RVB10010010080100100D0139RVB9410010092100100D0140RVB96100921009696D0141RVB98100941009090D0142RVB100100901008080D0143RVB809092909292D0144RVB929810090100100D0145RVB908210098100100D0146RVB9690100829696D0147RVB8698100908686D0148RVB98100969898100D0149RVB809890968086D0150RVB10096808610092D0151RVB10086929810098D0152RVB9098100809086D0153RVB98801001009892D0154RVB821009010090100D0155RVB90100989098100D0156RVB989082100100100D0157RVB10090909010098D0158RVB10096988010094D0159RVB100861008410084D0160RVB869886100100100D0161LKA90809010090100D0162LKA941009410096100D0163LKA100100100948690D0164LKA100901009498100D0165LKA10098100828090D0166LKA8282829810080D0167LKA909410010010084D0168LKA861009010090100D0169LKA1001009810098100D0170LKA100100848682100D0171LKA90821009010092D0172LKA928610094100100D0173LKA98989010096100D0198LKA86809210086100D0175LKA921009810098100D0176LKA10090868280100D0177LKA1008092100100100D0178LKA1008410090100100D0179LKA9810096929092D0180LKA9410086989294D0196LKA8410098869890D0182LKA1009480928692D0194LKA1009410010092100D0190LKA100928690100100D0196LKA100989896100100D0186LKA10086808690100D0192LKA9092100989296D0188LKA98100100809890D0198LKA10098901008680D0190LKA10094921009292D01100LKB9090921009090D0192LKB94100100988092D0180LKB100901001008498D0194LKB100801009010086D0190LKB10084988010092D0196LKB821009484100100D0184LKB1001008410094100D0198LKB9010010010094100D0186LKB989210010010098D0200LKB84100929410094D0201LKB100100100949284D0202LKB10010010010094100D0203LKB901001009290100D0204LKB921001009492100D0205LKB9810010090100100D0206LKB861009492100100D0207LKB929210010010090D0208LKB1009410010010098D0209LKB96909210096100D0210LKB86929410090100D0211LKB9810090968090D0212LKB8010092909294D0213LKB10010010080100100D0214LKB8610010092100100D0215LKB9896100100100100D0216LKB809010010010082D0217LKB1008096100100100D0218LKB1009290969490D0219LKB9010080909698D0220LKB9210092809884

AFP (2)Allele Frequencies by Population for Codominant Data*With Step by Step optionData SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Counts and Total Count by PopulationLocusAllele/nRVARVBLKALKBESTN6060606080453482123184202286144388100090796892634794716296250298497410025222527Locus2N60606060803434820230846212862260905107692555894624696032398357210030252229Locus3N6060606080334282101184211386544190468792688594131696243298655510030262528Allele Frequencies and Sample Size by PopulationsLocusAllele/nRVARVBLKALKBESTN30303030800.0670.0830.0500.067820.0170.0330.0500.017840.0330.0000.0330.033860.0170.0670.0670.050880.0170.0000.0000.000900.1170.1500.1000.133920.1000.0500.0670.117940.1170.0170.1000.033960.0330.0830.0000.033980.0670.1500.1170.0671000.4170.3670.4170.450Locus2N30303030800.0500.0670.0500.067820.0000.0330.0500.000840.1000.0330.0170.033860.0330.0330.1000.000900.0830.1670.1170.100920.0830.0830.0830.133940.1000.0330.0670.100960.0000.0500.0330.050980.0500.0830.1170.0331000.5000.4170.3670.483Locus3N30303030800.0500.0500.0670.033820.0170.0000.0170.017840.0330.0170.0170.050860.0830.0670.0670.017900.0670.1000.1330.117920.1000.1330.1330.083940.0170.0500.0170.100960.0330.0670.0500.033980.1000.0830.0830.0831000.5000.4330.4170.467

AGL (2)Allele Frequencies by Population with Graph over Loci for Codominant Data

Data SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies by PopulationsLocusAlleleRVARVBLKALKBEST800.0670.0830.0500.067820.0170.0330.0500.017840.0330.0000.0330.033860.0170.0670.0670.050880.0170.0000.0000.000900.1170.1500.1000.133920.1000.0500.0670.117940.1170.0170.1000.033960.0330.0830.0000.033980.0670.1500.1170.0671000.4170.3670.4170.450Locus2800.0500.0670.0500.067820.0000.0330.0500.000840.1000.0330.0170.033860.0330.0330.1000.000900.0830.1670.1170.100920.0830.0830.0830.133940.1000.0330.0670.100960.0000.0500.0330.050980.0500.0830.1170.0331000.5000.4170.3670.483Locus3800.0500.0500.0670.033820.0170.0000.0170.017840.0330.0170.0170.050860.0830.0670.0670.017900.0670.1000.1330.117920.1000.1330.1330.083940.0170.0500.0170.100960.0330.0670.0500.033980.1000.0830.0830.0831000.5000.4330.4170.467

AGF (2)Allele Frequencies with Graphs by Population and Locus for Codominant Data

Data SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies by Populations For Graph By LocusLocusAlleleRVARVBLKALKBEST800.0670.0830.0500.067820.0170.0330.0500.017840.0330.0000.0330.033860.0170.0670.0670.050880.0170.0000.0000.000900.1170.1500.1000.133920.1000.0500.0670.117940.1170.0170.1000.033960.0330.0830.0000.033980.0670.1500.1170.0671000.4170.3670.4170.450LocusAlleleRVARVBLKALKBLocus2800.0500.0670.0500.067820.0000.0330.0500.000840.1000.0330.0170.033860.0330.0330.1000.000900.0830.1670.1170.100920.0830.0830.0830.133940.1000.0330.0670.100960.0000.0500.0330.050980.0500.0830.1170.0331000.5000.4170.3670.483LocusAlleleRVARVBLKALKBLocus3800.0500.0500.0670.033820.0170.0000.0170.017840.0330.0170.0170.050860.0830.0670.0670.017900.0670.1000.1330.117920.1000.1330.1330.083940.0170.0500.0170.100960.0330.0670.0500.033980.1000.0830.0830.0831000.5000.4330.4170.467

EST AGP (2)Allele Frequencies at EST with Graphs by Population for Codominant Data

Data SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies by Population for ESTAlleleRVA800.067820.017840.033860.017880.017900.117920.100940.117960.033980.0671000.417AlleleRVB800.083820.033840.000860.067880.000900.150920.050940.017960.083980.1501000.367AlleleLKA800.050820.050840.033860.067880.000900.100920.067940.100960.000980.1171000.417AlleleLKB800.067820.017840.033860.050880.000900.133920.117940.033960.033980.0671000.450

Locus2 AGP (2)Allele Frequencies at Locus2 with Graphs by Population for Codominant Data

Data SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies by Population for Locus2AlleleRVA800.050820.000840.100860.033900.083920.083940.100960.000980.0501000.500AlleleRVB800.067820.033840.033860.033900.167920.083940.033960.050980.0831000.417AlleleLKA800.050820.050840.017860.100900.117920.083940.067960.033980.1171000.367AlleleLKB800.067820.000840.033860.000900.100920.133940.100960.050980.0331000.483

Locus3 AGP (2)Allele Frequencies at Locus3 with Graphs by Population for Codominant Data

Data SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies by Population for Locus3AlleleRVA800.050820.017840.033860.083900.067920.100940.017960.033980.1001000.500AlleleRVB800.050820.000840.017860.067900.100920.133940.050960.067980.0831000.433AlleleLKA800.067820.017840.017860.067900.133920.133940.017960.050980.0831000.417AlleleLKB800.033820.017840.050860.017900.117920.083940.100960.033980.0831000.467

AFL (2)Allele Frequencies by Locus for Codominant Data*With Step by Step optionData SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Allele Frequencies, Sample Size and Counts by LocusPopAllele/nESTCountLocus2CountLocus3CountRVAN306030603060800.06740.05030.0503820.01710.00000.0171840.03320.10060.0332860.01710.03320.0835880.017100900.11770.08350.0674920.10060.08350.1006940.11770.10060.0171960.03320.00000.0332980.06740.05030.10061000.417250.500300.50030RVBN306030603060800.08350.06740.0503820.03320.03320.0000840.00000.03320.0171860.06740.03320.0674880.000000900.15090.167100.1006920.05030.08350.1338940.01710.03320.0503960.08350.05030.0674980.15090.08350.08351000.367220.417250.43326LKAN306030603060800.05030.05030.0674820.05030.05030.0171840.03320.01710.0171860.06740.10060.0674880.000000900.10060.11770.1338920.06740.08350.1338940.10060.06740.0171960.00000.03320.0503980.11770.11770.08351000.417250.367220.41725LKBN306030603060800.06740.06740.0332820.01710.00000.0171840.03320.03320.0503860.05030.00000.0171880.000000900.13380.10060.1177920.11770.13380.0835940.03320.10060.1006960.03320.05030.0332980.06740.03320.08351000.450270.483290.46728

HFP (2)Heterozygosity, Fstatistics and Polymorphism by Population for Codominant Data*With Step by Step optionData SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Sample Size, No. Alleles, No. Effective Alleles, Information Index, Observed Heterozygosity, Expected and Unbiased Expected Heterozygosity, and Fixation IndexPopLocusNNaNeIHoHeuHeFRVAEST3011.0004.4891.8890.9000.7770.790-0.158Locus2308.0003.4481.6340.7670.7100.722-0.080Locus33010.0003.4881.7080.6330.7130.7250.112RVBEST309.0004.9591.8630.9000.7980.812-0.127Locus23010.0004.4121.8610.8000.7730.786-0.034Locus3309.0004.2251.7970.3670.7630.7760.520LKAEST309.0004.5001.8500.8330.7780.791-0.071Locus23010.0005.2791.9680.9330.8110.824-0.151Locus33010.0004.3801.8250.8000.7720.785-0.037LKBEST3010.0004.0181.7980.8000.7510.764-0.065Locus2308.0003.5641.6380.7330.7190.732-0.019Locus33010.0003.8381.7640.7330.7390.7520.008Mean and SE over Loci for each PopPopNNaNeIHoHeuHeFRVAMean30.0009.6673.8081.7440.7670.7340.746-0.042SE0.0000.8820.3400.0760.0770.0220.0220.080RVBMean30.0009.3334.5321.8410.6890.7780.7920.119SE0.0000.3330.2200.0220.1640.0100.0110.202LKAMean30.0009.6674.7191.8810.8560.7870.800-0.087SE0.0000.3330.2820.0440.0400.0120.0120.034LKBMean30.0009.3333.8071.7330.7560.7370.749-0.025SE0.0000.6670.1320.0490.0220.0090.0090.021Grand Mean and SE over Loci and PopsNNaNeIHoHeuHeFTotalMean30.0009.5004.2171.8000.7670.7590.772-0.009SE0.0000.2610.1660.0290.0440.0090.0100.053F-Statistics and Estimates of Nm over All Pops for each LocusAll Pops.LocusHtMean HeMean HoFisFitFstNmEST0.7830.7760.858-0.106-0.0960.00928.222Locus20.7630.7530.808-0.073-0.0600.01220.089Locus30.7510.7470.6330.1520.1570.00641.369

Mean-0.0090.0000.00929.893SE0.0810.0790.0026.200Percentage of Polymorphic Loci

Population%PRVA100.00%RVB100.00%LKA100.00%LKB100.00%Mean100.00%SE0.00%Na = No. of Different AllelesNe = No. of Effective Alleles = 1 / (Sum pi^2)I = Shannon's Information Index = -1* Sum (pi * Ln (pi))Ho = Observed Heterozygosity = No. of Hets / NHe = Expected Heterozygosity = 1 - Sum pi^2uHe = Unbiased Expected Heterozygosity = (2N / (2N-1)) * HeF = Fixation Index = (He - Ho) / He = 1 - (Ho / He)Where pi is the frequency of the ith allele for the population & Sum pi^2 is the sum of the squared population allele frequencies.Fis = (Mean He - Mean Ho) / Mean HeFit = (Ht - Mean Ho) / HtFst = (Ht - Mean He) / HtNm = [(1 / Fst) - 1] / 4Key: Mean He = Average He across the populations. Mean Ho = Average Ho across the populations.Ht = Total Expected Heterozygosity = 1 - Sum tpi^2 where tpi is the frequency of the ith allele for the total & Sum tpi^2 is the sum of the squared total allele frequencies.Note:Mean F-Statistics represent arithmetic averages. See G-Statistics for an alternative mean Fst estimate, calculated based on the average Hs and Ht over loci.

HFL (2)Heterozygosity, Fstatistics and Polymorphism by Locus for Codominant Data*With Step by Step optionData SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Sample Size, No. Alleles, No. Effective Alleles, Information Index, Observed Heterozygosity, Expected and Unbiased Expected Heterozygosity, and Fixation IndexPopESTLocus2Locus3RVAN303030Na11810Ne4.4893.4483.488I1.8891.6341.708Ho0.9000.7670.633He0.7770.7100.713uHe0.7900.7220.725F-0.158-0.0800.112RVBN303030Na9109Ne4.9594.4124.225I1.8631.8611.797Ho0.9000.8000.367He0.7980.7730.763uHe0.8120.7860.776F-0.127-0.0340.520LKAN303030Na91010Ne4.5005.2794.380I1.8501.9681.825Ho0.8330.9330.800He0.7780.8110.772uHe0.7910.8240.785F-0.071-0.151-0.037LKBN303030Na10810Ne4.0183.5643.838I1.7981.6381.764Ho0.8000.7330.733He0.7510.7190.739uHe0.7640.7320.752F-0.065-0.0190.008Mean and SE over Pops for each LocusESTLocus2Locus3NMean30.00030.00030.000SE0.0000.0000.000NaMean9.7509.0009.750SE0.4790.5770.250NeMean4.4914.1763.983SE0.1920.4260.200IMean1.8501.7751.773SE0.0190.0830.025HoMean0.8580.8080.633SE0.0250.0440.095HeMean0.7760.7530.747SE0.0100.0240.013uHeMean0.7890.7660.760SE0.0100.0240.013FMean-0.105-0.0710.151SE0.0220.0300.127Grand Mean and SE over Pops and LociTotalNMean30.000SE0.000NaMean9.500SE0.261NeMean4.217SE0.166IMean1.800SE0.029HoMean0.767SE0.044HeMean0.759SE0.009uHeMean0.772SE0.010FMean-0.009SE0.053F-Statistics and Estimates of Nm over All Pops for each LocusAll Pops.ESTLocus2Locus3Ht0.7830.7630.751Mean He0.7760.7530.747Mean Ho0.8580.8080.633MeanSEFis-0.106-0.0730.152-0.0090.081Fit-0.096-0.0600.1570.0000.079Fst0.0090.0120.0060.0090.002

Nm28.22220.08941.36929.8936.200Percentage of Polymorphic Loci

Population%PRVA100.00%RVB100.00%LKA100.00%LKB100.00%Mean100.00%SE0.00%Na = No. of Different AllelesNe = No. of Effective Alleles = 1 / (Sum pi^2)I = Shannon's Information Index = -1* Sum (pi * Ln (pi))Ho = Observed Heterozygosity = No. of Hets / NHe = Expected Heterozygosity = 1 - Sum pi^2uHe = Unbiased Expected Heterozygosity = (2N / (2N-1)) * HeF = Fixation Index = (He - Ho) / He = 1 - (Ho / He)Where pi is the frequency of the ith allele for the population & Sum pi^2 is the sum of the squared population allele frequencies.Fis = (Mean He - Mean Ho) / Mean HeFit = (Ht - Mean Ho) / HtFst = (Ht - Mean He) / HtNm = [(1 / Fst) - 1] / 4Key: Mean He = Average He across the populations. Mean Ho = Average Ho across the populations.Ht = Total Expected Heterozygosity = 1 - Sum tpi^2 where tpi is the frequency of the ith allele for the total & Sum tpi^2 is the sum of the squared total allele frequencies.Note:Mean F-Statistics represent arithmetic averages. See G-Statistics for an alternative mean Fst estimate, calculated based on the average Hs and Ht over loci.

APT (2)Allelic Patterns for Codominant Data*With Step by Step optionData SheetSheet1Data TitleAllozyme

No. Loci3No. Samples120No. Pops.4Totals for Allelic Patterns by PopulationsPopulationRVARVBLKALKBNa29282928Na Freq. >= 5%19.00021.00023.00018.000Ne1.0000.0000.0000.000No. LComm Alleles (