DR. Chip Biotech Inc. was founded at the Incubator Center of the Industrial Technology Research Institute (ITRI) in October 1998, with the goal of developing low cost and high quality of DNA chip products for clinical diagnosis. We continue improving and simplifying current biological research methods and clinical diagnostic tools to meet the demands of our customers. We have developed three technological platforms and filed thirty-nine patents in the US, EU, Japan, China, and Taiwan. Today DR. Chip develops and manufactures many biochip products, image analysis devices and software as well as biological reagents.

Microarray- revolutionary technology for research and

disease diagnosisMultiple targets (up to tens of thousands)SensitiveParallel comparisonProteins (peptides) or nucleotides

Microarray-barriers to commercializationHigh density, high costMainly for research and new drug

developmentRequiring expensive scannersNo standardized formatMicroarray companies mainly

Central technologyNucleic AcidExtraction

TargetAmplification

Probe&BiochipDesign

HybridizationTechnology

SignalDetection

Data Analysis

Our Current R&D Focuses

Polymer Microarray ChipsPolymer microarray chips are made of

various polymer materials and can be designed in different shapes and sizes

Oligonucleotide probes are designed to identify target sequences and unique sequence variances of various clinical samples

IntroductionDR. Holstein kit is a DNA-based system designed for identification of 4 common autosomal recessive genetic diseases, included bovine leukocyte adhesion deficiency (BLAD), citrullinaemia(CIT), deficiency of uridine monophosphatesynthase (DUMPS) and complex vertebral malfunction (CVM), in Holstein cattle in molecular level. The entire operation processes require 6 hours. DR. Holstein Kit adop ts t h ree mo lecu la r b io log i ca l techniques: genomic DNA extraction, PCR and DR. Chip’s proprietary nucleic acidhybridization technique on polymer chip.

Chip Images and Patterns

Principle of DR. Holstein kit

Procedure and Instruments

Health BLAD CIT DUMP CVM

Polymer chip characteristicLow noiseEasy to useCost saving

DR.Chip polymer chip (low noise)

Traditional membrane (high noise)

DR. Chip platform

DIY Chip (DR.AiM Platform)(96 well microplate)(DR.AiM Platform)

(96 well microplate)

1. Plastic adapter2. DIY kit3. 8x8 matrix

1 . H i g h T h r o u g h p u t S c r e e n i n g A r r a y ( 9 6 s a m p l e / b a t c h )

2.Automatic Analysis System

3. 6x6 matrix

Direction Marker

Hyb. Control probe

PCR control probe

DUMPS specific probe

CVM specific probe

BLAD specific probe

`

1 2 3 4 5A

B

C

D

E

CIT specific probe

G

DNA extraction

PCR

Hybridization

Colorimetric Development

Result Interpretation

TEL: 886-37-585585 . 0800-588858. FAX: 886-37-585586No.31,Ke Jung Rd.,Science-Based Industrial Park, Chu-Nan 350,Miao-Li County,Taiwan,R.O.C. DR.Chip Biotech, Inc. Copyright (c) 2008 all rights reserved.