dr. mahmud abuauba pct, dct connecting tubules cct. uriniferous tubule uriniferous tubule is the...
TRANSCRIPT
The Microscopic
Structure of the Kidney
Dr. Mahmud Abuauba MBChB, DCH, MD, PhD
Consultant Pediatrician & pediatric Nephrologist
Assistant Professor of Pediatrician
Zawia Kidney Center
5th
May 2018
الزاوية-ركز عالج أمراض الكلىم
برعاية
THE URINARY SYSTEM
Paired kidneys (2)
Paired ureters (2)
Unpaired urinary bladder (1)
Unpaired urethra (1)
SIX ORGANS
THE URINARY SYSTEM
THE KIDNEYS
CORTEX & MEDULLA
Coronal or long. section: two
regions:
Outer, darker, granular region
⚫ Renal cortex
⚫ Cortical labyrinth Cortical
⚫ Medullary rays arch
Inner lighter, striated region
⚫ Renal medulla ~10-18 medullary
pyramids
RENAL CORTEX
Renal lobule= medullary ray +neighbuoring cortical labyrinth,with medullary ray in the center oflobule bounded by interlobular As
Renal lobe= renal pyramid + renalcolumn + cortical arch
Renal lobe is a segment drains
through the renal papilla
RENAL MEDULLA
Composed of 10-18 conical-shapedmedullary tissue; renal or medullarypyramids
Base toward the CM border
Apex points toward the hilum, renalpapillae, project into minor calyx
Renal papillae perforated by 10-25opening of ducts of Bellini formingarea cribosa
Renal pyramids separated by cortical-like tissue (renal column of Bertin)
RENAL MEDULLA
Contains
Straight portion of PT & DT
Henle’s loop, ALH & DLH
Vasa recta
MCT
Papillary or ducts of Bellini
RENAL CORTEX
Cortical arch is portion of cortexoverlying renal pyramid
Formed of cortical labyrinth &medullary rays
Cortical labyrinth consists ofrenal corpuscle & convolutedtubules
Medullary ray, straight tubules ofseveral nephrons with single ormore collecting tubules
RENAL CORTEX
Contains
Glomeruli
PCT, DCT
Connecting tubules
CCT
URINIFEROUS TUBULE
Uriniferous tubule is the basic
functional unit of the kidney
Uriniferous tubule formed of
nephron & collecting tubule
UT entirely is epithelial in nature
UT are densely packed so the CT
of kidney (stroma) is scant
Uriniferous tubule
NEPHRON I
Nephron = renal corpuscle + renal
tubules
Each kidney has approx. a million
(0.7-1.3 million) nephrons
NEPHRON II
Several nephrons (10) are drainedby a single CCT
Several CCT join to form largertubule, MCT
Several MCT join to form ducts ofBellini, perforate & open at papilla
There are 250 collecting ducts
Each collects urine from 4000-5000 nephrons
NEPHRON III
Cclassifications
All nephrons are in the cortex
Cortical nephrons (85%); haveshort Henle’s loop, to outermedulla with short thin des HL &no thin Asc HL
⚫ Superficial nephrons
⚫ Mid-cortical nephrons
NEPHRON III
Juxtamedullary nephrons (15%=1/7);have very long Henle’s loop,sometimes descend to the tip ofpapilla
Have short thick des HL, long thinDec HL & thin Asc HL & thick Asc HL
To establish medullary hypertonicityfor concentrating urine
S1
S2S3
Cortical nephron
JM nephron
NEPHRON IV
Each nephron consists of:
⚫ Renal corpuscle
⚫ Renal tubule
RENAL CORPUSCLE
Each renal corpusclecconsists of:
⚫ Glomerulus, a tuft ofcapillaries
⚫ Bowman’s capsule, a double-walled epithelial capsule
Has two poles: vascular &urinary pole
Renal Corpusle
GLOMERULUS I
About 200-250 m
A tuft of capillaries
Invaginated into Bowman’s
capsule
Formed from AGA
AGAs are short, stright, enter
renal corpuscle at vascular pole
Filtrating surface area is 0.8 m2
GLOMERULUS II
Each AGA divides into 3-5branches; into a tuft of capillaries(20-40 loops) → glomerulus
Fenestrated endothelial cells (70-90 nm); no diaphragm, act as aphysical barrier
Capillaries coalesce again to formefferent glomerular arteriole
EGA is longer & narrower
Bowman’s capsule
Consists of two layers:
⚫ Visceral layer; internal; modified
epithelial cells called podocytes
⚫ Parietal layer; external, single layer
of squamous epith supported by
basal lamina & reticular fibers
In between, Bowman’s or urinary
space, receives ultrafiltrate
Neck of the PT at urinary pole
PODOCYTES
Modified epithelial cells of
visceral layer
Have cell body & primary
processes (arise from body) both
not in contact with the capillary
Secondary processes or pedicels
arise from the primary processes
Pedicels in intimate contact with
LRE
FILTRATION SLIT
Pedicels interdigitate forming
filtration slit≈ 25 nm (20-40) &
covered by slit diaphragm≈ 6 nm
thick
SD has circular pores of 3-5 nm
Acts as a physical barrier
Pedicels covered by glycocalyx
Glycocalyx contains sialoprotein
(podocalyxin)
Filtration Slit
Proteinuria
GLOMERULAR BASEMENT
MEMBRANE
Is produced by fusion of basal lamina
of endothelial cells & podocytes
As a physical & charge barrier
Consists of three layers:⚫ Lamina rara interna
⚫ Lamina densa; collagen IV
⚫ Lamina rara externa
Laminin, fibrenectin, heparan
sulphate
FILTRATION BARRIER
Is a barrier between blood in
capillaries & Bowman’s space
Consists of three layers:
⚫ Fenestrated endothelial cells
⚫ GBM
⚫ Filtration slits of podocytes
⚫ Glycocalyx
FILTRATION BARRIER
Lamina densa is a meshwork of
type IV collagen & laminin in a
matrix containing –vely charged
proteoglycan (heparan sulfate)
acts as a charge & physical
barriers
Laminae rarae contain fibronectin
that binds them to cells
FILTRATION BARRIER
Particles >10 nm in diameter don’t
cross GBM
While –vely charged proteins with
MWt > 69 kDa pass sparingly
The largest protein succeeded to
cross GBM has MWt 70kDa,
therefore small amounts of
albumin appear in urine
MESANGIUM
Located between glomerular
capillaries & consists of:
⚫ Mesangial matrix & mesangial cells
Mesangial cells:
⚫ Intraglomerular mesangial cells
⚫ Extraglomerular mesangial cells
Supporting, phagocytosis,
contraction, resorption
Mesangial cells
They are contractile & have Ang II
receptors that when activated the
glomerular blood flow is reduced
Also contain ANF receptors that
relax mesangial cells & ↑ RBF
Therefore, they regulate the RBF
& glomerular filtration
RENAL TUBULE
The second part
of the nephron
RENAL TUBULE
Is the part of uriniferous tubule
orginates at the urinary pole of
the renal corpuscle
Divided into 12 segments
Proximal tubule
⚫ S1, 1st 2/3 of para convoluta
⚫ S2, last 1/3 of para convoluta & much of para recta
⚫ S3, remainder of para recta
Loop of Henle
⚫ Thin descending limb
⚫ Thin ascending limb
Distal tubule
⚫ Medullary thick ascending limb
⚫ Cortical thick ascending limb
⚫ Distal convoluted tubule
Connecting tubule
Collecting tubule or duct
⚫ Cortical collecting tubule
⚫ Outer medullary collecting tubule
⚫ Inner medullary collecting tubule
S1
S2S3
Cortical nephron
JM nephron
S1
S2
S3
PROXIMAL TUBULE I
First part of renal tubule
Drains the urinary space
⚫ Pars convoluta = PCT
⚫ Pars recta = straight PT = DTkLH
Divides into three segments:
⚫ S1;1st 2/3 of pars convoluta
⚫ S2; last 1/3 of PC & much of PR
⚫ S3; remainder of pars recta
PROXIMAL TUBULE II
Confined to renal cortex
Is longer than DCT therefore more
numerous on histological cross section
It is surrounded by peritubular
capillaries
Composed of single layer of cuboidal
epithelial cells
PROXIMAL TUBULE III
Have brush border of microvilli & apical
canaliculi in between
These canaliculi help in formation of
pinocytosis to absorb macromolecules
as proteins
PROXIMAL TUBULE IV
Rich in mitochondria and it is highly
metabolically active,mainly S1
Because basolateral membranes have
abundant Na-K pump which
responsible for active reabsorption of
Na ions back into peritubular capillaries
HENLE’S LOOP
A U-shaped structure starts as
continuation of PR & ends as ATkLHL
Consists of three (or 5) parts:
⚫ Descending thick LHL
⚫ Descending thin LHL
⚫ Loop or turn of Henle
⚫ Ascending thin LHL
⚫ Ascending thick LHL
composed of single squamous
HENLE’S LOOP
HENLE’S LOOP
1/7 are JM nephrons
Consists of four types of cells:
l Type I only in midcortical &
superficial nephrons
l Type II,III,IV in JM nephrons
JM nephrons have long LHL
Cortical nephrons have short LHL
DISTAL TUBULE
Starts as continuation of ATnLHL
Subdivided into three parts:
⚫ Pars recta = ATkLHL (part of HL)
⚫ Macula densa
⚫ Pars convoluta = DCT
DT has single cuboidal epithelial
cells, no brush border, no canaliculi
ATkLHL is highly metabolic
DISTAL TUBULE
Macula densa
⚫ At junction of ATkLHL & DCT
⚫ It is part of juxtaglomerular
apparatus
DCT
⚫ From MD to connecting tubule
⚫ Cudoidal cells, No brush border
⚫ Shorter than PCT, ratio 1:7
Macula densa
Is a part of DT at junction of
ATkLHL & DCT
⚫ Also part of juxtaglomerular
apparatus
Consists of modified columnar
cells with closely packed nuclei
Sensitive to ionic content & water
of tubular fluid
Ju
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CONNECTING TUBULE
It is a short segment of renaltubules that connects DCT tocortical collecting tubules
DCT of several nephrons join toform short connecting tubule
Several connecting tubules join toform collecting tubule
S1
S2S3
Cortical nephron
JM nephron
S1
S2
S3
COLLECTING TUBULE
Consists of three parts:
⚫ CCT
⚫ IMCT
⚫ OMCT (papillary duct, Bellini ducts)
It is not part of the nephronembryologicaly different
The epithelium of CT is sensitiveto vasopressin or ADH to ↑ waterreabsorption
Development of the renal pelvis, calyces, collecting
tubules
A: at 6 weeks, B: at the end of the 6th
wk, C: 7 weeks
& D: newborn
COLLECTING TUBULE
CCT consists of two types of cells:⚫ Principal cells (P cells), Na reabsorption
& water reabsorption 2ndary to ADH
⚫ Intercalated cells (I cells); AB balance
MCT formed by union of severalCCT; has same cells as CCT
Papillary CT or ducts of Belliniformed by union of several MCT &open at area cribosa; has onlyprincipal cells
Prof. Dr. Peter AGRE
Nobel Laureate
Nobel Prize in
Chemistry 2003
RENAL INTERSTITIUM
It is the space between uriniferous
tubules & blood & lymphatic
vessels
Composed of:
⚫ scanty loose CT; collagen & elastic
fibers in both cortex & medulla
⚫ Three types of cells; fibroblast;
macrophages & interstitial cells
RENAL INTERSTITIUM
Interstitial cells have numerous
lipid droplets & synthesize &
secrete medullipin I
Medullipin I is converted (liver) to
medullipin II; a potent vasodialtor
which lowers BP
Also secrete PGs (E2), prostcyclin
JUXTAGLOMERULAR
APPARATUS
Is a combination of specialized
tubular & vascular cells located at
vascular pole of glomerulus
Consists of:
⚫ Juxtaglomerular cells of the AGA
⚫ Macula densa of the distal tubule
⚫ Extraglomerular mesangial or Lacis
cells
Juxtaglomerular apparatus
Ju
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Juxtaglomerular apparatus
JUXTAGLOMERULAR CELLS
Specialized, modified (VSMC)
myoepithelaial cells; mainly
tunica media of AGA as it enters
glomeruls, occasionally EGA
Contain secretory granules; renin
Renin is a proteolytic enzyme that
converts angoitensinogen into
angiotensin I to regulate BP
JUXTAGLOMERULAR CELLS
JGCs receive sympathetic nerve
via 1 adrenergic receptors
Acts as a baroreceptor (BP)
Sensitive to change of BP
So regulate arterial blood
pressure
MACULA DENSA
Is a speciliazed short segment of
DT, as ATkLHL passes near its
own corpuscle
Located at vascular pole between
AGA & EGA
Composed of modified epithelial
cells that are tall, narrow & have
dense closely packed nuclei
Appears as dark spot by LM
MACULA DENSA
They are not innervated
Act as chemoreceptors (Na&Cl)
Sensitive to Na & Cl load in DT
The JG cells & MD cells are in
intimate contact, because no
basal lamina between them
LACIS CELLS
Extraglomerular mesangial cells
Polkissen or polar cushions
Are one type of mesangial cells
forming interstitium of JGA
Located between AGA, EGA & MD
Are in intimate contact with
intramesangial cells
Function is unknown (renin)
Bladder & Urinary passages
Conduct & store urine till excreted to
exterior
Mucosa of calyces, renal pelvis,
ureters, UB consists of transitional
epit with basal lamina
Muscle fibers of UB run in every
direction until reach bladder neck
then arranged into 3 layers
Adventia covers them externally
Bladder & Urinary passages
Internal long layer becomes circular
around prostatic urethra, true
involuntary urethral sphincter
It extends to external meatus in ♀
Middle layer ends at bladder neck
Outer layer ends at prostate in ♂ & at
external meatus ♀
THANKS FOR
ATTENTION