CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

Dr.Chen Gao

cg_npwr@yahoo.com.cn

DNA的提取与含量测定

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

DNA ， DNP

变性蛋白，肝糜

氯仿

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

( 二 ) 二苯胺法测定DNA含量

二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和

分别针对 DNA和 RNA的颜色反应方法

本实验采用针对 DNA的二苯胺法测 DNA的含量。在酸性溶液中， DNA与二苯胺共热生成蓝色化合物，该物质在 595nm有最大吸收，在 40～ 400mg/mL范围内其峰值与 DNA含量成正比

NH

100℃

冰醋酸，少量硫酸蓝色物DNA +