新開発の浮遊細胞固定化ディッシュ - nof...bam-protein conjugate...
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![Page 1: 新開発の浮遊細胞固定化ディッシュ - NOF...BAM-Protein Conjugate 細胞固定化の新技術 NOF CORPORATION 浮遊細胞の固定化方法 1st Pre-coated BAM 2nd Addition](https://reader034.vdocuments.pub/reader034/viewer/2022050400/5f7e3aa6ccb2456197168a61/html5/thumbnails/1.jpg)
NOF CORPORATION
新開発の浮遊細胞固定化ディッシュ
1) 細胞ダメージの無い新規開発浮遊細胞固定化技術2) 簡単に使えるプレコートガラスボトムディッシュ3) 浮遊細胞の各種顕微鏡観察に使用可能
ex. 細胞内カルシュームの観察 Green Fluorescence Protein (GFP) の局在化の観察 微速度撮影を用いた細胞増殖の観察
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Physiologycally active substances
Biocompatible Anchor for Membrane (BAM)
Oleyl groupPolyetyleneglycol chain
X : Reactive group
OO X
n
Oleyl-O-PEG-X - BAM
Protein,Enzyme,Drug etc.BAM-ProteinConjugate
細胞固定化の新技術
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NOF CORPORATION
浮遊細胞の固定化方法
1st Pre-coated BAM
2nd Addition of cell suspension
At r.t. for 10 min(until cells settle on the BAM surface)
cell
wash
3rd Addition of culture medium Observe
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細胞固定化方法の比較
Slide glassBSA coating
BAM
+ - - --+ + +
++ ++++ +
+ + -
BAM modified surface PLL-coated surfaceNOF Reference
Membrane-active immobilization Electrostatic immobilization
Adsorbed PLLSlide glass
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1. Apply the treated cell suspension (50µl) by Fura-3(4µM).2. Wash with PBS to remove non-immobilized cells.3. After 10 minutes, apply the calcium ionophore, A23187(50µM,100µl), to the
immobilized cells.
BAM coated glass
Wash with PBS to remove non-immobilized cells
Fura3-treated cell suspension
K562細胞における細胞内カルシウムの観察
Add A23187
Phase contrast view of cell immobilization using BAM
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NOF CORPORATION
Calcium Ionophore
添加前
添加後
蛍光顕微鏡像光学顕微鏡像
K562細胞における細胞内カルシウムの観察
(Data from Prof. Nagamune, Department of Chemistry and Biotechnolog, School of Engineering, The University of Tokyo, Japan).