首都大学東京 理工学研究科 生命科学年報

2016

生命科学教室 http://www.biol.se.tmu.ac.jp/

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a) Role of the kinase activity of LMTK1A: LMTK1A, a neuronal kinase, is a regulator of vesicle trafficking and

neurite growth, however how its kinase activity plays a role in such regulation was not clear. I found that kinase active

LMTK1A inhibits vesicle transition from actin to microtubules at neurite tip. I also found that LMTK1A regulates

cellular microtubule organization. (Govinda)

b) LMTK1 LMTK1 LMTK1

c) BACE1 LMTK1 x

APP BACE1 LMTK1

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Phos-Tag 12 Thr181, Ser202,

Thr231,Ser235,Ser404 ( )

c) Developmental changes in tau isoforms and their phosphorylation: Isoforms and phosphorylation of tau change

during brain development. To know how the changes in isoforms and phosphorylation of tau are related, I analyzed

phosphorylation of 3-repeats and 4-repeats tau in mouse brains from perinatal days 3 to 30 in detail by Phos-tag

SDS-PAGE, and found that the change in phosphorylation proceeded without the isoform change. (Tuerde) d) Quantitative study on cellular activation of GSK3β: Activity of GSK3β is regulated by autophosphorylation at

Tyr216 and inhibitory phosphorylation at Ser9. I examined whether the kinase activity of GSK3β could be measured

quantitatively by Phos-tag SDS-PAGE, and fount it possible. I measured GSK3b activitiy in neurons and brains, and

found that most of GSK3b in brains are active and is not suppressed by the insulin-Akt signaling. (Krishnankutty)

e) Tau phosphorylation in different tauopathies: Tau hyperphosphorylation are found in different tauopathies but it is

not clear how different pathologies result from same causative protein. Using post-mortem human brain, I have found

that the pattern of tau phosphorylation is different in different tauopathies. (Sharma)

Mechanisms underlying neurodegeneration caused by disruption of mitochondrial distribution and

Alzheimer’s disease-related protein tau (Ando, Hayashishita, Oka)

a) Age-dependent changes in ATP homeostasis in the axon and cell body in brain neurons (Oka): Metabolic

processes and energy demand change during aging, however, it is not understood whether and how basal ATP levels

changes in neurons during aging in the brain and how those changes affects neuronal vulnerability. Using transgenic

Drosophila expressing fluorescent ATP sensor, we found that ATP levels decrease in the cell body but not in the axon

during aging. These results suggest that the maintenance mechanisms of ATP levels are different between cell body and

axon during aging.

b) Roles of CaMKII in tau-induced neurodegeneration (Oka): Accumulation of tau is associated with

neurodegenerative diseases including Alzheimer’s disease (AD), while how abnormal tau causes neurodegeneration is

not fully understood. We found that elevated activity of Ca2+/calmodulin-dependent protein kinase II (CaMKII)

promotes neurodegeneration caused by tau.

c) Mechanisms by which Alzheimer’s disease-related protein tau gains toxicity and accumulates in disease brains

(Hayashishita): Abnormal accumulation of the microtubule-interacting protein tau causes neurodegenerative diseases

including Alzheimer’s disease (AD), however, the mechanisms underlying tau accumulation and toxicity remain

unclear. Using transgenic Drosophila expressing human tau as a model, we identified novel pathway that can reduce tau

accumulation and toxicity through tau phosphorylation at AD-related sites.

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1) Furusawa, K., Asada, A., Urrutia, P., Gonzalez-Billault, C., Fukuda, M., Hisanaga, SI. Cdk5 Regulation of the

GRAB-Mediated Rab8-Rab11 Cascade in Axon Outgrowth. J Neurosci. 37: 790-806., 2017.

2) Akasaka-Manya, K., Kawamura, M., Tsumoto, H., Saitoh, Y., Shinobu Kitazume, S., Hatsuda, H., Miura, Y.,

Hisanaga, S., Murayama, S., Hashimoto, Y., Manya, H., Endo, T. Excess APP O-glycosylation by GalNAc-T6

decreases Aβ production. J. Biochem. 161:99-111, 2017

3) Ando, K., Oka, M., Ohtake, Y., Hayashishita, M., Shimizu, S., Hisanaga, S., Iijima, K. M. Tau phosphorylation at Alzheimer's disease-related Ser356 contributes to tau stabilization when PAR-1/MARK activity is elevated. Biochem. Biophys. Res. Commun. 478:929-934. 2016.

4) Kimura, T., Hosokawa, T., Taoka, M., Tsutsumi, K., Ando, K., Ishiguro, K., Hosokawa, M., Hasegawa, M.,

Hisanaga, S. Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17

mutants. Sci. Rep. 6:33479, 2016.

5) Sharma, S., Tsutsumi, K., Saito, T., Asada, A., Ando, K., Tomomura, T., Hisanaga, S. The kinase activity of endosomal kinase LMTK1A regulates its cellular localization and interactions with cytoskeletons. Genes Cells. 21: 1080-1094. 2016.

6) Tarutani, A., Suzuki, G., Shimozawa, A., Nonaka , T., Akiyama, H., Hisanaga, S., and Hasegawa, M. Effect of

Fragmented Pathogenic α-Synuclein Seeds on Prion-like Propagation. J. Biol. Chem., J. Biol. Chem.,

291:18675-18688. 2016.

7) Oikawa, T., Nonaka, T., Hisanaga, S., Hasegawa, M. (2016) a-Synuclein fibrils exhibit gain-of-toxic-function, promoting tau aggregation and inhibiting microtubule assembly. J Biol Chem. 291:15046-15056, 2016

8) Shimonaka, S, Nonaka, T, Suzuki, G, Hisanaga, S, Hasegawa, M. Templated Aggregation of TAR DNA-binding

Protein of 43 kDa (TDP-43) by Seeding with TDP-43 Peptide Fibrils. J Biol Chem. 291:8896-907. 2016

9) Takasugi, T., Minegishi, S., Asada, A., Saito, T., Kawahara, H., Hisanaga, S. Two degradation pathways of the p35

Cdk5 activation subunit, dependent and independent of ubiquitination. J. Biol. Chem. 291:4649-4657, 2016. 10) Kimura, T., Hatsuta, H., Masuda-Suzukake, M., Hosokawa, M., Ishiguro, K., Akiyama, H., Murayama, S., Hasegawa,

M., Hisanaga, S. The abundance of nonphosphorylated tau in mouse and human tauopathy brains revealed by the use

of Phos-tag method. Am. J. Pathol. 186:398-409, 2016.

11) Ando K, Maruko-Otake A, Ohtake Y, Hayashishita M, Sekiya M, and Iijima KM. Stabilization of microtubule-unbound tau via tau phosphorylation at Ser262/356 by Par-1/MARK contributes to augmentation of AD-related phosphorylation and Aβ42-induced tau toxicity. PLoS Genetics 12(3): e1005917, 2016.

12) Katow, S., Katow, T., Yoshida, Y., Kiyomoto, M., Uemura, I. Immunohistochemical and ultrastructural properties of the larval ciliary bandassociated strand in the sea urchin Hemicentrotus pulcherrimus. Frontiers in Zoology 13:27, 2016.

13) . 20 (2),129-135, 2016.

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17) Krishnankutty, A, Kimura, T., Saito, T., Asada, A., Ishiguro, K., Hisanaga, S. Quantitative measurement of in vivo GSK3B activity using Phos-tag technique. 14th meeting of APSN, (Aug. Kaula Lumpur, Malaysia).

18) Tuerde, D., Kimura, T., Miyasaka, T., Asada, A., Saito1, T.,Ando, K., Hisanaga, S. Phosphorylation and Isoform Expression of Microtubule–Associated Protein Tau are Regulated Independently during Brain Development. Asian-Pacific Society for Neurochemistry, (Aug. Kuala Lumpur, Malaysia)

19) Kimura, T., Ishiguro, K., Hasegawa, M., Hisanaga, S. Heterogeneous phosphorylation of microtubule-associated protein tau in cultured cells. 14th meeting of APSN, (Aug. Kaula Lumpur, Malaysia).

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2 23) Oka, M., Suzuki, E., Hisanaga, S.., Iijima, K. M, Ando., K. Reduction in ATP levels in the axon during aging and the

role of mitochondrial distribution.” 59th Annual Meeting of The Japanese Society for Neurochemistry (Sep.

Fukuoka)

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Ser262 38

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25) Oka, M., Suzuki, E., Hisanaga, S., Iijima, K. M, and Ando., K. Reduction in ATP levels in the axon during aging and

the role of mitochondrial distribution. Society for Neuroscience’s the 46th annual meeting, (Nov. San Diego, USA,)

26) Ando, K., Maruko-Otake, A., Hayashishita, M., Oka, M., Ohtake, Y., Sekiya, M., Saito, T., Hisanaga, S., Iijima, K.

M. Sustained activation of CaMKII caused by depletion of mitochondria from the axon enhances tau toxicity. 46th

annual meeting of Society for Neuroscience (Nov. San Diego, USA)

27) Krishnankutty, K., Kimura, T., Saito, T., Aoyagi, K.,Asada, A., Ando, K., Ohara-Imaizumi, M., Ishiguro, K., Hisanaga, S. In vivo regulation of GSK3b activity unveiled by the quantitative measurement of its phospho-isotypes. 46th annual meeting of Society for Neuroscience (Nov. San Diego, USA)

28) Furusawa, K., Asada, A., Urrutia, P., Gonzalez-Billault, C., Fukuda, M., Hisanaga, SI. Cdk5-dependent

phosphorylation of GRAB, a guanine nucleotide exchange factor for Rab8, regulates axon outgrowth by directing

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Rab8A to Rab11A-positive endosomes. American Society for Cell Biology 2016 (12 )

29) Tuerde, D., Kimura, T., Miyasaka, T., Asada, A., Saito1, T., Ando, K., Hisanaga, S. Phosphorylation and Isoform Expression of Microtubule–Associated Protein Tau are Regulated Independently during Brain Development. The Molecular Biology Society of Japan, (Dec. Yokohama, Japan)

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2. 1) Serotonin transporter in Drosophila

The neurotransmitter serotonin has been implicated in behavior and many neuropsychiatric disorders. However, its

precise roles are not well understood. We generated a null mutation in SerT encoding a serotonin transporter in

Drosophila using the CRISPR/Cas9 system, and found that the mutant flies showed abnormal locomotor activity and

sleep ( , ).

2) Succinyl CoA synthetase in Drosophila

Scsα (Succinyl-CoA synthetase alpha subunit) encodes a subunit of SCS, a TCA cycle enzyme and its deficiency is

known to cause severe metabolic disease in humans. We have generated a null mutation ScsαKO in Drosophila. The

mutant flies showed developmental delays, locomotor activity defects and the glycogen breakdown at the beginning of

the third instar larval stage. The mutant should be useful as a model to understand the physiological role of SCSα in vivo.

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19) Dynamics of chromatin structure and epigenetic modification in Drosophila oocytes

The Drosophila oocyte nucleus undergoes dynamic changes in chromatin structure during late prophase. We found

that changes in histone methylation and acetylation are related to transient chromatin decondensation observed in stage

10 oocytes. We also performed genetic screening for epigenetics-related genes which play important role in regulation

of chromatin dynamics in germline cells ( , ).

3.

1. Thoa TT, Minagawa N, Aigaki T, Ito Y, Uzawa T. (2017) Regulation of photosensitization processes by an RNA

aptamer. Sci Rep. 7:43272.

2. Quan X, Sato-Miyata Y, Tsuda M, Muramatsu K, Asano T, Takeo S, Aigaki T. (2017) Deficiency of succinyl-CoA

synthetase α subunit delays development, impairs locomotor activity and reduces survival under starvation in

Drosophila. Biochem Biophys Res. Commun. 483, 566-571.

3. Tanegashima K, Sato-Miyata Y, Funakoshi M, Nishito Y, Aigaki T, Hara T. (2017) Epigenetic regulation of the

glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice.

Gene Cells. 22,71-83.

4. Manandhar Y, Wang W, Inoue J, Hayashi N, Uzawa T, Ito Y, Aigaki T, Ito Y. (2017) Interactions of in vitro selected

fluorogenic peptide aptamers with calmodulin. Biotechnol Lett. 39, 375-382.

5. Tsuda M, Aigaki T. (2016) Evolition of sex-peptide in Drosophila. Fly (Austin) 10, 172-7.

6. Kohda M, Tokuzawa Y, Kishita Y, Nyuzuki H, Moriyama Y, Mizuno Y, Hirata T, Yatsuka Y, Yamashita-Sugahara Y,

Nakachi Y, Kato H, Okuda A, Tamaru S, Borna NN, Banshoya K, Aigaki T, Sato-Miyata Y, Ohnuma K, Suzuki T,

Nagao A, Maehata H, Matsuda F, Higasa K, Nagasaki M, Yasuda J, Yamamoto M, Fushimi T, Shimura M,

Kaiho-Ichimoto K, Harashima H, Yamazaki T, Mori M, Murayama K, Ohtake A, Okazaki Y. (2016) A

Comprehensive Genomic Analsis Reveals the Genetic Landscape of Mitochondreal Respiratory Chain Complex

Deficiencies. PLoS Genet. 12(1):e1005679.

7. #Shimada N, #Inami S, Sato S, Kitamoto S, and Sakai T. (2016) Modulation of light-driven arousal by

LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain.

Scientific Reports. 6: 37255. (# co-first author)

8. #Mabuchi I, #Shimada N, Sato S, Ienaga K, Inami S, *Sakai T. (2016) Mushroom body signaling is required for

locomotor activity rhythms in Drosophila. Neuroscience Research 111: 25-33. (# co-first author)

2' +)*/ ★ .*

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y23y

1. Nakayama, M., Cattani, M., Cruz, I., Trindade, L., Sato-Miyata, Y., and Aigaki, T. (2016) How can we avoid

Caffeine-induced sleep disturbances? ~ Guarana, a highly caffeinated plant from the Amazon may help~. World

Congress on Brain, Behavior and Emotions. June 12-15, Buenos Aires, Argentina

2. Yamamoto, M., Kaneuchi, T., Sato-Miyata, Y., Takeo, S. and Aigaki, T. (2016) Metabolomic analysis of

egg activation in Drosophila. Insect Reproductive Molecules Meeting, June 28 - July 1. Münster, Germany

3. Ohnuma, K., Sato-Miyata, Y., Kishita, Y., Kohda, M., Murayama, K., Ohtake, A., Okazaki, Y. and Aigaki, T, (2016)

Investigating Mitochondrial Respiratory Chain Disorders (MRCD) in Drosophila, The Allied Genetics Conference.

July 13-17, Orlando, Florida, USA

4. Ohnuma, K., Sato-Miyata, Y., Kishita, Y., Nyuzuki, H. Kohda, M., Murayama, K., Ohtake, A., Okazaki, Y. and

Aigaki, T. (2016) A Novel Function of dSki3, a Drosophila homologue TTC37 related to Trichohepatoenteric

Syndrome. The 12th Japan Drosophila Research Coference, Sep. 9-11, Tokyo

5. Yamamoto, M., Kaneuchi, T., Sato-Miyata, Y., Takeo, S. and Aigaki, T. Metabolomic analysis of egg activation in

Drosophila. The 12th Japan Drosophila Research Coference, Sep. 9-11, Tokyo

6. Ohnuma, K., Sato-Miyata, Y., Kishita, Y., Nyuzuki, H. Kohda, M., Murayama, K., Ohtake, A., Okazaki, Y. and

Aigaki, T. (2016) Deficiency of ski3 gene impairs mitochondrial function and lipid metabolism, 39

11 30 12 2 ,

7. , (2017) SP SP . 2016

2 7 8 ,

8. Ohashi H., Sakai T. (2016) A neuropeptide signaling regulates starvation-dependent reduction of heat nociception in

Drosophila. Neuroscience2016 (Annual meeting of Society for Neuroscience), San Diego, 2016/11/12-16

9. Sato S., Ueno K., Sakai T. (2016) cAMP production induced by AL-MB synaptic transmission reduces Ca2+

responses in Drosophila MB. 39 7 22 .

10. Ohashi H., Sakai T. (2016) The exploration of neuropeptide involved in starvation-dependent reduction of heat

nociception in Drosophila. 39 7 21 .

11. Inami S., Shimada N., Asano T., Sakai T. (2016) Drosophila LIM homeodomain transcription factor Apteorus

regulates acquisition and maintenance of long-term memory. 39 7 21 .

12. Mabuchi I., Ienaga K., Sakai T. (2016) The circadian clock gene, period, in the dorsal lateral neurons is critically

involved in Drosophila long-term memory. 39 7 21 .

13. Ohashi H., Sakai T. (2016) Brain is requires for starvation-dependent reduction of heat nociception in Drosophila

adults. JSCPB △ "Environmental Sensing and Animal Behavior" 6 10 .

14. 2016 ★ 2016 9 , 15. Tsunaki Asano (2016) "Impact of laccase-mediated cuticle formation on evolution of insects" Joint event of

22nd International Congress of Zoology and 87th Meeting of Zoological Society of Japan, 11 ,

16. 2017 ★ , 87 ,

3 ,

17. 2017 , 61 , 3

,

y24y

1. Aigaki, T. (2016) Molecular evolution of the SP/SPR system in Drosophila. Insect Reproductive Molecules Meeting,

June 28 – July 1. Westfälische Wilhelms-University, Münster, Germany

2. Aigaki, T. (2016) Nutrition and lifespan. Amazonian Meeting of Geriatrics and Gerontology, Nov. 8, Manaus, Brazil

3. (2016) 20����������� 12 17 ,

,

4. Asano, T and Kanost, M (2016)rlaccase activation during molting process”, at Symposium “Insect Biocomposites:

Cuticles and Peritrophic Matrices”, in International Congress of Entomology, Sept, Orlando, USA.

Arakane, Y., Noh, M. Y., Asano, T., Kramer, KJ. (2016) "Tyrosine Metabolism for Insect Cuticle Pigmentation and

Sclerotization" in "Extracellular Composite Matrices in Arthropods", Springer, ISBN-10: 3319407384

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1. Watanabe, K., Tominaga, K., Kitamura, M., and Kato, J. (2016) Systematic identification of synthetic lethal

mutations with reduced-genome Escherichia coli: synthetic genetic interactions among yoaA, xthA, and holC related

to survival of MMS exposure. Genes Genet. Syst. 91: 183-188.

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Watanabe, M., Ehira, S., Suzuki, E., Awai, K., Nakamura, Y. (2016) CyanoBase: A large-scale update on its 20th

anniversary. Nucleic Acids Res. 45:D551-D554.

3. Ohbayashi, R., Watanabe, S., Ehira, S., Kanesaki, Y., Chibazakura, T., Yoshikawa, H. (2016) Diversification of

DnaA dependency for DNA replication in cyanobacterial evolution. ISME J. 10:1113-1121.

4. (2016) .

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Kanno Y., Oikawa T., Chiba Y., Ishimaru Y., Shimizu T., Sano N., Koshiba T., Kamiya Y., Ueda M., Seo M. (2016)

AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes. Nat Commun. 7: 13245. Matsumura T., Okamoto T. (2016) Isolation of gametes from Brachypodium distachyon. Plant Biotech. 33: 39-43. Ohnishi Y., Okamoto T. (2017) Nuclear migration during karyogamy in rice zygotes is mediated by continuous

convergence of actin meshwork toward the egg nucleus. J. Plant Res., in press Okamoto T. (2017) Analysis of proteins enriched in rice gamete. Methods Mol. Biol. in press Okamoto T., Ohnishi Y., Toda E. (2017) Development of polyspermic zygote and possible contribution of polyspermy to

polyploid formation in angiosperms. J. Plant Res., in press Park K., Kim Y., Vickers M., Park JS., Hyun Y., Okamoto T., Zilberman D., Fischer R., Feng X., Choi Y., Scholten S.

(2017) DNA demethylation is initiated in the central cells of Arabidopsis and rice. Proc. Natl. Acad. Sci. USA 113: 15138–15143.

Suzuki H., Yokawa K., Nakano S., Yoshida Y., Fabrissin I., Okamoto T., Baluška F., Koshiba T. (2016) Root cap-dependent gravitropic U-turn of maize root requires light-induced auxin biosynthesis via the YUC-pathway in the root apex. J. Exp. Bot. 67: 4581–4591.

Takeuchi K., Hasegawa H., Gyohda A., Komatsu S., Okamoto T., Okada K., Terakawa T., Koshiba T. (2016) Overexpression of RSOsPR10, a root-specific rice PR10 gene, confers tolerance against drought stress in rice and drought and salt stresses in bentgrass. Plant Cell Tissue Organ Cult. 127: 35-46.

Toda E., Ohnishi Y., Okamoto T. (2016) Development of polyspermic rice zygotes. Plant Physiol. 171: 206-214. Toda E., Ohnishi Y., Okamoto T. (2016) Electro-fusion of gametes and subsequent culture of zygotes. Bio Protocol 6:

e2074. Toda E., Okamoto T. (2016) Formation of triploid plants via possible polyspermy. Plant Signal. Behav. 11: e1218107. Yokawa K., Baluška F. (2016) TOR complex: switch for emergency in root behavior. Plant Cell Physiol. 57: 14-18.

Ⅲ 80 (9 )

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in vitro 1309

μ in vitro 2016

Shimizu T., Masujima T., Koshiba T., Seo M. “Current situation of quantitative single cell plant hormone analysis by mass spectrometry.” 58 Ⅲ 3

Suzuki H., Fujita C., Kimura T., Sakai T., Isobe T., Taoka M., Okamoto T., Koshiba T. (2017) “Analysis of fluense-dependent phosphorylation of Zmphot1 in phototropism of maize coleoptiles. 58 Ⅲ

3 Suzuki H., Yokawa K., Okamoto T., Baluška F., Koshiba T. Light and root cap-dependent gravitropism of maize root

requires auxin biosynthesis. TMU bio-confference 2016

2016 58 Ⅲ

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131 3 ⑤ Ⅲ

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Ⅲ 2017- 15371

Ⅲ 2016-70288

2016 11 18-20

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1. Suzuki, R. and Kawahara, H. (2016) UBQLN4 recognizers mislocalized transmembrane domain proteins and targets

these to proteasomal degradation. EMBO Rep. 17, 842–857. doi 10.15252/embr.201541402

2. Tanaka, H., Takahashi, T., Xie, Y., Minami, R., Yanagi, Y., Hayashishita, M., Suzuki, R., Yokota, N., Shimada, M.,

x38x

Mizushima, T., Kuwabara, N., Kato, R., and Kawahara, H. (2016) A conserved island of BAG6/Scythe is related to

ubiquitin domains and participates in short hydrophobicity recognition. FEBS J. 283 (2016) 662–677. doi:

10.1111/febs.13618

3. Takasugi, T., Saito, T., Asada, A., Kawahara, H. and Hisanaga, S.-I. (2016) Two degradation pathways of the p35

Cdk5 activation subunit, dependent and independent of ubiquitination.

J. Biol. Chem. 291, 4649-4657. doi: 10.1074/jbc.M115.692871

4. Yamaki, Y., Kagawa, H., Hatta, T., Natsume, T., and Kawahara, H. (2016) The C-terminal cytoplasmic tail of

hedgehog receptor Patched1 is a platform for E3 ubiquitin ligase complexes.

Mol. Cell. Biochem. 414: 1-12. doi:10.1007/s11010-015-2643-4

5. 2016

The World of UBIQUITIN, Vol. 4, p.60-64.

6. 2016 88 3 p.426

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Fraimout A, Debat V, Fellous S, Hufbauer RA, Foucaud J, Pudlo P, Marin J-M, Price DK, Cattel J, Chen X, Deprá M,

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Shearer P, Singh N, Tamura K, Xuéreb A, Zhang J, Estoup A. (2017) Deciphering the routes of invasion of

Drosophila suzukii by means of ABC random forest. Mol. Biol. Evol. (in press)

Igawa T, Nozawa M, Suzuki DG, Reimer JD, Norov AR, Wang Y, Henmi Y, Yasui K. (2017) Evolutionary history of

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the extant amphioxus lineage with shallow-branching diversification. Sci. Rep. (in press)

Mello B, Tao Q, Tamura K, Kumar S. (2017) Fast and accurate estimates of divergence times from big data. Mol. Biol.

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Y, Hoshi Y, Imai T, Kasahara M, Librado P, Mao L, Mori H, Nishiyama T, Nozawa M, Palfalvi G, Pollard ST,

Rozas J, Sanchez-Gracia A, Sankoff D, Shibata TF, Shigenobu S, Sumikawa N, Uzawa T, Xie M, Zheng C, Pollock

DD, Albert VA, Li S, Hasebe M. (2017) Genome of the pitcher plant Cephalotus reveals genetic changes associated

with carnivory. Nature Ecol. Evol. 1:0059.

Kudo A, Shigenobu S, Kadota K, Nozawa M, Shibata FT, Ishikawa Y, Matsuo, T. (2017) Comparative analysis of the

brain transcriptome in a hyper-aggressive fruit fly, Drosophila prolongata. Insect Biochem. Mol. Biol. 82:11-20.

Okamiya H, Igawa T, Nozawa M, Sumida M, and Kusano T. (2017) Development and characterization of 23

microsatellite markers for the montane brown frog (Rana ornativantris) Curr. Herpetology. 36:63-68.

Kumar S, Stecher, G, Tamura K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger

Datasets. Mol. Biol. Evol. 33:1870-1874.

Sunaga, S., Akiyama, N., Miyagi, R., and Takahashi, A. (2016) Factors underlying natural variation in body

pigmentation of Drosophila melanogaster. Genes Genet. Syst. 91: 127-137.

Yoshida, K., Miyagi, R., Mori, S., Takahashi, A., Makino, T., Toyoda, A., Fujiyama, A., and Kitano, J. (2016) Whole

genome sequencing reveals small genomic regions of introgression in an introduced crater lake population of

threespine stickleback. Ecol. Evol. 6: 2190-2204.

Nozawa M, Onizuka K, Fujimi M, Ikeo K, Gojobori T. (2016) Accelerated pseudogenization on the neo-X chromosome

in Drosophila miranda. Nat. Commun. 7:13659.

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and Algae. Mol. Phyl. Evol. 103:19-25.

Ishikawa M, Yuyama I, Shimizu H, Nozawa M, Ikeo K, Gojobori T. (2016) Different endosymbiotic interactions in two

hydra species reflect the evolutionary history of endosymbiosis. Genome Biol. Evol. 8:2155-2163.

Nozawa M, Fujimi M, Iwamoto C, Onizuka K, Fukuda N, Ikeo K, Gojobori T. (2016) Evolutionary transitions of

microRNA-target pairs. Genome Biol. Evol. 8:1621-1633.

Koga H, Fujitani H, Morino Y, Miyamoto N, Tsuchimoto J, Shibata TF, Nozawa M, Shigenobu S, Ogura A, Tachibana

K, Kiyomoto M, Amemiya S, Wada H. (2016) Experimental approach reveals the role of alx1 in the evolution of the

echinoderm larval skeleton. PLOS One 11:e0149067.

Nozawa M, Kinjo S. (2016) Origin and evolution of non-coding RNAs. Encyclopedia Evol. Biol. 3:130-135. Kojima KK, Seto Y and Fujiwara H (2016) The Wide Distribution and Change of Target Specificity of R2 Non-LTR

Retrotransposons in Animals. PLoS ONE 11:e0163496, 2016

Tamura K (2017) Divergence time estimation using genome-scale sequence data. Symposium on "Genome Evolution at

Mishima" (Mishima)

Seto Y and Tamura K (2016) Comparative RNA-seq analysis of antifungal immune responses among Drosophila

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species. SMBE 2016, Annual Meeting of the Society for Molecular Biology and Evolution (Gold Coast)

Takahashi, A. (2016) Genetics underlying species diversification: cases in Drosophila "Biology of biodiversity"

Commemorative Symposium for the 32nd International Prize for Biology (Tokyo).

Akiyama N, Miyagi R, Takahashi A 2016 Genetic bases of the association between body color variation and

environmental stress tolerances in Drosophila melanogaster 12

Muto L, Kamimura Y, Tanaka K, Takahashi A 2016 Coevolution of male and female genitalia in Drosophila suzukii

driven by changes in ovipositor shape 12

Tanaka K, Takahashi A 2016 The genetic basis of rapid morphological diversification of male genitalia 12

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Tanaka, K. Ito, S. and Kurokawa, M. 2016 Central pathways of the hindgut movement in the penaeid shrimp,

Marsupenaeus japonicus. Kyorin J. Arts and Sciences, 33 1-7

Okutani, M. and Kurokawa, M. (2016) Dual innervation by central and peripheral nervous system in feeding behavior

of Bivalvia, Mytilus. Environmental Sensing and Animal Behavior

Okutani, M. and Kurokawa, M. (2016) Monoaminergic regulation of ciliary movements of the labial palps in Mytilus

galloprovincialis. The 22nd International Conference of Zoology and The 87th Meeting of Zoological Society of

Japan

Syu, G. and Kurokawa, M. (2016) Rest in Aplysia kurodai is a sleep-like state. The 22nd International Conference of

Zoology and The 87th Meeting of Zoological Society of Japan

Kurokawa, M. (2016) Comparative physiology of regional specificity of the enteric nervous system in some

gastropods. in Symposium entitled Digestive Tract of Metazoans: Its Diversity and Evolution. The 22nd

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y53y

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(2017) 58

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y54y

o t Environmental Microbiology 1. Members

③

D2 D2 D2 M2 M2

M2 M2 M1 M1 M1 M1 Chen

Yuxin M1 B4

2. Research

×

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…

Survival strategy and ecophysiology 1-a. Rhodopseudomonas palustris −

Stress tolerance induced by carbon-starvation in purple photosynthetic bacterium, Rhodopseudomonas

palustris

Bacteria in natural environments are frequently exposed to nutrient starvation. In order to give an energetic insight

into survivability under starved conditions, this study employed a purple photosynthetic bacterium, Rhodopseudomonas

palustris, and compared cellular responses to starvation in the light and the dark, with special focus on salt stress

tolerance.

R. palustris was anaerobically cultivated in a carbon-limited medium with illumination. Growth was terminated at

exponential growth phase. The growth-terminated cells were further incubated for one day in the light and the dark.

After exposure to 2.5 M NaCl stress for 1 h, the survival ratio determined from colony forming units was 11% and 14%

in the exponentially growing cells and the growth-terminated cells, respectively. In 1-d starved cells in the light, the salt

tolerance drastically increased (80% of survival ratio), intracellular ATP contents increased, and the number of cells

with shorter length obviously increased. On the other hand, 1-d starved cells in the dark showed decreased ATP

contents and did not increase the salt tolerance (16%). No marked cell-shortening was observed in 1-d starved cells in

the dark. These results indicate ATP produced by photosynthetic energy conversion in starved cells contributes to salt

tolerance. These findings suggest that ATP supply makes starved cells adaptive to salt stress. *

1-b.

Survival of thermophilic cyanobacteria beyond its growing temperature

Survivability of bacteria at high temperature environments which are unfavorable to their growth has importance to

y55y

understand bacterial behavior in nature. This study investigated the distribution and survivability of cyanobacteria at

high temperature area in Nakabusa hot springs. A piece of microbial mats developed at various temperatures were

collected. Fluorescent intensity of phycobilin per protein contents was determined. The relative values to that of the

56°C sample as 100 were 68, 0.5, 0.2, and 0.07 for the 62°C, 63°C, 66°C, and 76°C sample, respectively. Cultivation

experiments at 55°C in light indicated the existence of viable unicellular cyanobacteria at 80°C. But cyanobacterial

growth was not detected from any samples after cultivation at 65°C.

Small population of cyanobacteria existed beyond its growing temperature and maintained its viability in Nakabusa

hot springs. These results suggest that cyanobacteria have an ability to survive at fluctuating temperatures.

1-c.

Diversity of thermophilic cyanobacteria in iron rich hot springs

Iron rich environments have been studied as model systems analogous to the ancient sea. These studies will elucidate

the involvement of microbes in ferrous iron oxidation, both today and throughout the early earth. This study explored

the diversity of cyanobacteria in an iron rich hot spring, Jinata hot spring in Shikine Island, one of Izu Islands in Tokyo.

Microbial mats were observed on the surface of sediments in the hot spring water: pH was approximately 6,

temperature was 50-55°C, and the reduced iron concentrations were around 200 µM. Molecular ecological analyses

indicated the existence of three species of cyanobacteria, Leptolyngbya sp., Thermosynechococcus sp., and Pannus sp.

A pecies of the microbial mats was cultivated under photoautotrophic conditions using ASNIII medium at 45°C or

BG11 medium at 50°C. Morphological analyses of the obtained cultures indicated the growth of these three species of

cyanobacteria. Our results suggest that these cyanobacteria grow and survivie in the iron rich hot springs and evolved

oxygen by the cyanobacteria is involved in the iron sedimentation. (Idei, Matsuura, and McGlynn*)

1-d.

Nitrogen fixing properties of chemolithoautotrophic microbial mats in terrestrial hot springs

Nitrogen-fixing activities at thermal environments are poorly understood. This study characterized nitrogen-fixing

activity of chemolithoautotrophic microbial mats developed at 74°C at sulfidic and slightly alkaline hot spring water.

Nitrogenase activity was determined using the acetylene reduction assay.

Nitrogenase activities were detected in conditions where moderate amounts of methane were produced suggesting

that nitrogen fixation occurs within a limited range of redox levels in the communities. Addition of molybdate, an

inhibitor of anaerobic sulfur metabolisms which have sulfite as an intermediate (e.g., sulfate reduction, and sulfur

disproportionation), inhibited the nitrogenase activity of the microbial mats. Dispersion of cell aggregates of the mats

also decreased the nitrogenase activity, but the activity was partially recovered by amendment with H2 and CO2. These

suggest that the nitrogenase activity in the mats is coupled with hydrogen-autotrophic and anaerobic sulfur metabolisms.

In addition, cell aggregation may contribute to efficient interspecies interactions supporting nitrogen fixation. Taken

together, our findings provide new insight into the ecological contributions of anoxic sulfur metabolisms in

chemosynthetic thermophilic microbial communities. (Nishihara, Thier*, McGlynn*, Haruta, and Matsuura)

1-e.

Diversity and function of cellulose-utilizing bacteria in hot spring microbial mats

y56y

It has been reported that several types of microbial mats in hot springs contain cellulose. Extracellular cellulose

produced by bacteria may work to form microbial mats. It seems that chemolithoautotrophic and photosynthetic bacteria

are the possible members synthesizing bacterial cellulose. However, diversity of cellulose-utilizing bacteria in hot

spring microbial mats has not been studied well and their function in the microbial ecosystems has not been clarified yet.

This study is trying to isolate and characterize cellulose-utilizing bacteria in the microbial mats.

Several types of microbial mats were collected from Nakabusa hot springs. A piece of the mats was inoculated into

cultivation media containing cellulose powder and anaerobically cultivated at 60°C. Repetitive cultivations are being

conducted to isolate cellulose-utilizing bacteria. In addition, cellulose-utilizing ability of Fervidobacterium sp. which

has been isolated from the hot spring is being tested. This fermentative bacterial species is one of the dominant species

in the microbial mats. (Chen, Matsuura, and Haruta)

1-f.

Abundance and diversity of aerobic anoxygenic photosynthetic bacteria in epilithic biofilm

AAPB

a

bacteriochlorophyll a (BChl a)

BChl a) (AAPB %) 26.1 %

70.5 % AAPB % a (

a ) 16S rRNA

alpha-4 Alphaproteobacteria

Gammaproteobacteria a

AAPB a

a AAPB * *

Interspecies interaction 2-a.

Co-existence stimulates biofilm formation of unicellular cyanobacterium and filamentous anoxygenic

photosynthetic bacterium

A unicellular cyanobacterium, Thermosynechococcus sp. is widely found in multispecies biofilms attaching on solid

surfaces in hot springs. It has been reported this cyanobacterium doesn’t have cell-aggregating and attaching abilities in

axenic cultures under its growth conditions. This study investigated biofilm formation of Thermosynechococcus sp. by

focusing on effects of other bacteria. A pecies of cyanobacteria-dominated thick biofilms, i.e., microbial mats were

collected from Nakabusa hot springs and were cultivated photoautotrophically. After repetitive cultivations, bacteria

were isolated from the greenish biofilm on the cultivation flask under heterotrophic dark conditions. Co-cultivation

experiments of the isolates with Thermosynechococcus sp. strain NK55a found one of the isolates, strain F1 formed

biofilms with the cyanobacterium. 16S rRNA gene sequence analysis of strain F1 indicated that this strain was closely

related to a filamentous photosynthetic bacterium, Chloroflexus aurantiacus. Similar biofilm formation was observed by

co-cultivation with Chloroflexus aggregans.

These results found that Themosynechococcus sp. and Chloroflexus sp. formed biofilms under co-culture conditions.

y57y

It is likely that Chloroflexus sp. receive organic carbons and stimulate biofilm-formation in the mixed culture.

2-b.

Growth of protease-producing bacteria supported by thermophilic cyanobacteria of hot spring microbial mats

Dense microbial communities called microbial mats are developed in natural environments including hot springs.

Protease-producing bacteria are frequently isolated from the hot spring microbial mats. It has not been clarified how the

protease-producing bacteria grow on and affect a primary producer, Thermosynechococcus sp. in the mats.

Thermosynechococcus sp., a dominant species of the microbial mats in Nakabusa hot spings was cultivated, and the

culture solution was used to prepared agar plates. Seven strains of protease producing bacteria which had been isolated

from the microbial mats were cultivated on the agar plate. After two days of cultivation at 55°C in the dark,

colony-formation was confirmed in all the strains tested. To evaluate susceptibility of the cyanobacterial cells to

proteolysis, a paper disk containing a purified protease was put on the cyanobacteria-containing agar plate. After 24

hours of incubation, no clear discoloration of greenish color was observed in the agar plate. These results indicate that

protease-producing bacterial isolates utilize extracellular nutrients produced by Thermosynechococcus sp. but do not

lyze the cyanobacterial cells.

2-c. Chloroflexus aggregans

Exploration of bacteria attracting filamentous gliding bacterium, Chloroflexus aggregans

Chloroflexus aggregans

C. aggregans

C.

aggregans C. aggregans C.

aggregans ─

*

2-d.

Sulfide production in sulfur oxidizing bacteria-dominated microbial mats

Microbial mats are developed in sulfidic hot spring waters at Nakabusa hot springs. At over 75°C, two types of mats

are observed: whitish and grayish ones. Both are dominated by sulfur oxidizing bacteria coexisting with various bacteria.

In order to elucidate the sulfur cycles in the mats, this study comparatively analyzed sulfide producing abilities of these

two mats.

Microbial mats were anaerobically incubated in artificial hot spring water and sulfide concentrations were

periodically determined. During the incubation, sulfide concentrations were increased in both types of the mats, and the

amount of sulfide produced in the whitish mats was higher than that in the grayish mats. These results indicated that

differences in sulfate-reducing and sulfur-reducing abilities of these mats. Environmental factors such as oxygen supply

may determine the sulfur metabolisms of the mats.

y58y

2-e.

Growth suppression of green algae by aerobic heterotrophic bacteria

Chlorella vulgaris

4 4

×

16S rRNA Pseudomonas

Pseudomonas

/

* Collaborators

Vera Their

Shawn McGlynn

4. Publications

Fukushima, S., S. Morohoshi, S. Hanada, K. Matsuura and S. Haruta. Gliding motility driven by individual cell-surface

movements in a multicellular filamentous bacterium Chloroflexus aggregans. FEMS Microbiol. Lett., 363:fnw056

(2016).

Hirose, S., K. Matsuura, S. Haruta. Phylogenetically diverse aerobic anoxygenic phototrophic bacteria isolated from

epilithic biofilms in Tama River, Japan. Microbes Environ., 31:299-306 (2016).

Haruta, S., Y. Saito, and H. Futamata. Editorial: Development of microbial ecological theory: stability, plasticity and

evolution of microbial ecosystems. Front. Microbiol., (2016).

Haruta, S., T. Iino, M. Ohkuma, K. Suzuki, and Y. Igarashi. Ca2+ in hybridization solutions for fluorescence in situ

hybridization facilitate the detection of Enterobacteriaceae. Microbes Environ., in press (2017).

Books

+)*/

Presentations

89 (2016)

Idei, A., T. Kakegawa, SE. McGlynn. Possible Fe(II) Fueled Microbial Ecosystem at the Carbonate Rich Hot Spring

Okuoku-Hachikuro Onsen, an Early Ocean Analog Site. 26th Goldschmidt Conference (Yokohama) (2016)

y59y

2016

2016

2016

Arai, S., N. Kanno, K. Matsuura, S. Haruta. Carbon-starvation-induced changes in cellular state was affected by

illumination in purple photosynthetic bacterium Rhodopseudomonas palustris. 16th International Symposium on

Microbial Ecology (Montreal, Canada) (2016)

Nishihara, A., M. Labiad, S. McGlynn, K. Matsuura, S. Haruta. Detection of nitrogenase activity and nifH genes in

microbial streamer communities in sulfidic hot springs at Nakabusa, Japan. 31

(2016)

Rhodopseudomonas palustris −

31 (2016)

31 (2016)

Chloroflexus aggregans

31 (2016)

Idei, A., K. Matsuura, S. McGlynn. Microorganism iron particle interactions in a Fe(II) rich hot spring. 31

(2016)

31 (2016)

31

(2016)

31 (2016)

90

Sleeping Microbes: (2017)

y60y

t u 1.

Phan Quoc Toan D3 9 D2 DC M2

9 D1 10 M2 M2 M2 Pino Blanco Ana M1 9

M2 10 M1 M1 M1 M1

2. ×

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2016

(1) (a)

Callosciurus finlaysonii

× Ⅱ ×

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3.

1. Boonkhaw, P., Prayoon, U., Kanchanasaka, B., Hayashi, F. and Tamura, N. (2017) Colour polymorphism and genetic

relationships among twelve subspecies of Callosciurus finlaysonii in Thailand. Mammalian Biology 85: 6-13.

2. Hashimoto, K. and Hayashi, F. (2016) Cantharidin world on islands: Species diversity of canthariphilous arthropods

in the Izu-Ogasawara Arc. Entomological Science 19: 432-439.

3. Hashimoto, K., Sugawara, H. and Hayashi, F. (2016) Sclerotised spines in the female bursa associated with male’s

spermatophore production in cantharidin-producing false blister beetles. Journal of Insect Physiology 93: 18-27.

4. Hashimoto, K., Suzuki, K. and Hayashi, F. (2016) Unique set of copulatory organs in mantises: concealed female

genital opening and extremely asymmetric male genitalia. Entomological Science 19: 383-390.

y62y

5. Ishikawa, N. F., Hayashi, F., Sasaki, Y., Chikaraishi, Y., Ohkouchi, N. (2017) Trophic discrimination factor of

nitrogen isotopes within amino acids in the dobsonfly Protohermes grandis (Megaloptera: Corydalidae) larvae in a

controlled feeding. Ecology and Evolution 7: 1674-1679.

6. Ito, M., Seto, N., Rico, B. Shigeta, M., Tamura, N. and Hayashi, F. (2016) Folivory with leaf folding by giant flying

squirrels: its patterns and possible function. Ecological Research 31: 617-626.

7. Karube, H. and Phan, Q. T. (2016) Discovery of Chlorogomphus papilio Ris, 1927 from northern Vietnam. TOMBO

58: 49-51.

8. Kishimoto, K. and Hayashi, F. (2017) The complete embryonic and larval stages of the oophagous frog Kurixalus

eiffingeri (Rhacophoridae). Current Herpetology, 36: 37-45.

9. 2016 Ⅱ

2016: 135-146.

10. Niitsu, S., Sugawara, H. and Hayashi, F. (2017) Evolution of female-specific wingless forms in bagworm moths.

Evolution & Development 19: 9-16.

11. Okamiya, H., Igawa, T., Nozawa, M., Sumida, M. and Kusano, T. (2017) Development and characterization of 23

microsatellite markers for the montane brown frog (Rana ornativentris). Current Herpetology 36: 63-68.

12. Phan, Q. T. and Dinh, T. P. A. (2016) Odonata from the Cham Islands, off central Vietnam, collected in September

2015. Journal of the International Dragonfly Fund. Faunistic Studies in SE Asian and Pacific Island Odonata 13:

1-22.

13. Phan, Q. T. and Kompier, T. (2016a) A study of the genus Protosticta Selys, 1855, with descriptions of four new

species from Vietnam (Odonata: Platystictidae). Zootaxa 4098 (3): 529-544.

14. Phan, Q. T. and Kompier, T. (2016b) Description of two new species of Coeliccia from Vietnam (Odonata:

Platycnemididae). Zootaxa 4196 (3): 407-414.

15. 2016 Rana ornativentris

Ⅱ 2016: 122-130

16. Sugawara, H., Kusano, T. and Hayashi, F. (2016) Fine-scale genetic differentiation in a salamander Hynobius

tokyoensis living in fragmented urban habitats in and around Tokyo, Japan. Zoological Science 33: 476-484.

17. Boonkhaw, P., Prayoon, U., Budsabong, K., Tamura, N. and Hayashi, F. (2016) Present situation and conservation

concerns in Callosciurus finlaysonii in Thailand. 2016

18. 2017

64

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24. 2017

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26. Murakami, Y., Tsujimura, M., Kato, H. and Hayashi, F. (2016) Flower visiting by the gecko Lepidodactylus

lugubris in the Ogasawara Islands. The 8th World Congress of Herpetology, Hangzhou, China.

27. 2017

64

28. , 2016

Ⅱ 55

29. Okamiya, H., Shiga, Y., Sugawara, H. and Kusano, T. (2016) Which factors influence genetic diversity and

reproductive success in populations of endangered Japanese amphibians? The 8th World Congress of

Herpetology, Hangzhou, China.

30. , , 2017

64

31. Pino Blanco, A. and Hayashi, F. (2017)

64

32. Boonkhaw, P. Kanchanasaka, B. 2017 Ⅰ

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33. 2017 52 (1): 4-7

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Science of the Total Environment, 545-546, 372-380.

DOI: 10.1016/j.scitotenv.2015.12.007

Ⅲ 1997-1999

1978 1991

2003 2012 2014

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y67y

2003 2003 Ⅲ

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Ⅲ

Weed Research, 56,

168-178. DOI: 10.1111/wre.12198

Ⅲ (http://www.biol.se.tmu.ac.jp/plantecol/)

3. 2016 5 13

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4.

y68y

Hosaka, N., Kachi, N., Kudoh, H., Stuefer, J.F. & Whigham, D. (2016) Compensatory growth of the clonal understory

tree, Asimina triloba, in response to small-scale disturbances. Plant Ecology, 217, 471-480

Hata, K., Kawakami, K. & Kachi, N. (2016) Increases in soil water content after the mortality of non-native trees in

oceanic island forest ecosystems are due to reduced water loss during dry periods. Science of the Total

Environment 545-546: 372-380

Osawa, T., Hata, K. & Kachi, N. (2016) Eradication of feral goats enhances expansion of the invasive shrub Leucaena

leucocephala L. on Nakoudo-jima, an oceanic island. Weed Research 56: 168-178

(2016) ♂

. 9: 119-124

2016 Ⅲ Ⅲ pp.158-159

2016 Ⅲ Ⅲ pp.252-253

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2016 pp.259-261

2016 Ⅲ Ⅲ pp.244-245

△

Tsunoda T., Suzuki J.-I. & Kaneko N., Fatty acids analyses to detect feeding behaviour of a generalist root chewing

insect, Anomala cuprea (Coleoptera: Scarabaeidae) larva. XVII International Colloquium on Soil Zoology, Nara,

Japan, August 2016

Inouye K., Ikeda H., Niwa S., Minamiya Y., Tsunoda T., Miura T. & Kaneko N., Soil foodweb structure as an indicator

of nitrogen use efficiency in conservation agriculture. XVII International Colloquium on Soil Zoology, Nara,

Japan, August 2016

Iwasaki A., Ito T., Nemoto R., Hashimi R., Komatsuzaki M., Kaneda S., Tsunoda T. & Kaneko N., Effects of no tillage

with clipped mulch on PLFA profiling of microbial community structure and nematode community. XVII

International Colloquium on Soil Zoology, Nara, Japan, August 2016

Sangat B., Kaneko N., Minamiya Y., Tsunoda T. & Kimura J., Nitrogen dynamics on no till weed management under

wheat plantation. XVII International Colloquium on Soil Zoology, Nara, Japan, August 2016

Tsunoda T., Makoto K., Suzuki J.-I. & Kaneko N., Temperature warming enhanced damage of an omnivorous

root-chewing insect (Anomala cuprea (Coleoptera: Scarabaeidae)) on Lolium perenne (Poaceae) growth but did

not affect the insect behaviour and growth. The 13th Gordon Research Conference on Plant-Herbivore Interactions,

Ventura, USA, February 2017

64 × 4

http://www.biol.se.tmu.ac.jp/plantecol/

y69y

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Cronin • 2015 8

y70y

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y71y

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y72y

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A) ABS Access and

Benefit-Sharing

URA

B) 11 12

y73y

3.

1. Eguchi K, Mizuno R, Ito F, Satria R, Dang VA, Bui TV, Phung THL (2016) First Discovery of subdichthadiigyne in Yunodorylus Xu (Formicidae: Dorylinae), Revue suisse de Zoologie, 123, 307-314.

2. Hashimoto Y, Endo T, Itioka T, Hyodo F, Yamasaki T, Mohamed M (2016) Pattern of co-occurrence between ant-mimicking jumping spiders and sympatric ants in a Bornean tropical rainforest. Raffles Bulletin of Zoology, 64, 70-75.

3. Kobayashi H, Nonaka M (2016) Molecular phylogeny of the Notodontidae: Subfamilies inferred from 28S rRNA sequences (Lepidoptera, Noctuoidea, Notodontidae), Tinea, 23 Supplement 1, 1-83.

4. Kurushima H, Yoshimura J, Kim Je, Kim Jo, Nishimoto Y, Sayama K, Kato M, Watanabe K, Hasegawa E, Roff DA, Shimizu A (2016) Co-occurrence of ecologically equivalent cryptic species of spider wasps. Royal Society Open Science, 3, 160119; DOI: 10.1098/rsos.160119: 1-10.

5. Niitsu S, Jinbo U, Nasu Y (2016) The first discovery of genus Narycia (Lepidoptera, Psychidae) from Japan, with description of a new species. Nota Lepidopterologica 39 (2), 137-143.

6. Niitsu S, Sugawara H, Hayashi F (2017) Evolution of female-specific wingless forms in bagworm moths. Evolution & Development 19 (1), 9-16.

7. Phung THL, Yamasaki T, Eguchi K (2016) Conspecificity of Phintella aequipeiformis Zabka 1985 and P. lucai Zabka 1985 (Araneae: Salticidae), confirmed by DNA barcoding. Revue suisse de Zoologie, 123, 283-290.

8. Satria R, Bui TV, Eguchi K (2017, online first) New synonymy and redescription of Anochetus

mixtus Radchenko, 1993, and distinction from the other members of the Anochetus rugosus group (Hymenoptera: Formicidae: Ponerinae). Asian Myrmecology, 9.

9. Satria R, Sasaki O, Bui TV, Oguri E, Syoji K, Fisher BL, Yamane S, Eguchi K (2016) Description of the first Oriental species of the ant genus Xymmer (Hymenoptera: Formicidae: Amblyoponinae). Zootaxa, 4168, 141-150.

10. Syoji K, Eguchi K (2017, online first) Nesting site selection of Temnothorax congruus from Japanese broad-leave forests: a trade-off between nest site quality and distance from sympatric Crematogaster teranishii colonies. Asian Myrmecology 9.

11. Yamasaki T, Hashimoto Y, Endo T, Hyodo F, Itioka T (2016) A new species of the genus Castoponera (Araneae, Corinnidae) from Sarawak, Borneo, with comparison to a related species. ZooKeys, 596, 13-25.

12. (2016) DNA pp.184-190.

―

13. Cronin AL, Loeuille N, Monnin T (2017) The competition-colonisation trade-off, harlequin landscapes, and the evolution of alternative reproductive strategies in ants. 64th meeting of the

y74y

Ecological Society of Japan. Tokyo March 14-19, 2017. 14. Kurushima H & Shimizu A 2016 Discovery of multiple cryptic species of the Auplopus

carbonarius species-complex (Hymenoptera: Pompilidae). 25 △

15. Phung THL , Yamasaki T, Eguchi K (2016) Taxonomic study of Vietnamese species of the genus

Phintella (Araneae: Salticidae). 48th Annual meeting of Arachnological society of Japan (Kashiwa Campus of the University of Tokyo, Chiba).

16. Phung THL , Yamasaki T, Eguchi K (2017) Taxonomic study of the genus Lechia Zabka, 1985

(Araneae: Salticidae). 2nd Meeting for young arachnologists (University of Tokyo). 17. Shimizu A , Kurushima K 2016 Brood parasitism in spider wasps, with special reference to its

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