effect of volatile on fungal spore germination 周秋伶 90134086 2004/12/28

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Effect of volatile on fungal spore germination 周周周 90134086 2004/12/28

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Effect of volatile on fungal spore germination

周秋伶 90134086

2004/12/28

前言• Fusarium oxysporum (尖孢鐮刀菌)• 大孢子 鐮刀型 3~ 5 個隔膜 31.75 ×4.38

mm

• 小孢子 單胞,長圓筒型至紡錘形 8.25 ×2.5mm

• 厚膜孢子 壁厚,近圓形 9.45 ×8.13mm

目的

揮發性物質對真菌孢子的影響

空培養皿

水 Acetate Methanol Ethanol

1% 5% 25%

V 型玻棒

玻片

30 1℃ 天三重複

流程

揮發性物質測試之裝置

玻片

孢子懸浮液

V 形玻棒

結果

圖一 . Fusarium oxysporum 大分生孢子 (左 )與小分生孢子(右 )

圖二 . 萌芽的 Fusarium oxysporum 小分生孢子

表一、 不同濃度揮發性物質對 Fusarium oxysporum 孢子萌芽影響

揮發性物質(%)

Acetate Ethanol Methanol

Control

1 5 25 *1 5 25 1 5 25

萌芽數(spore) 335 0 0 0 298 297 0 240 203 0

萌芽率(%) 71 0 0 0 66 58 0 53 38 0

*α=0.01 時,與 control 組無顯著差異

討論• 孢子懸浮液濃度• 孢子接觸揮發性物質面積• 揮發性物質 pH 值• 揮發性物質濃度

參考文獻 • Rai, M.K., and D. Acharya. 1999. Screening of some As

teraceous plants for antimycotic activity. Compositae Newsletter. 34:37-43

• Xavier, L.J.C., and J.J. Germida. 2003. Bacteria associated with Glomus clarum spores influence mycorrhizal activity. Soil Biology and Biochemistry. 35:471-478

• Marullo, P., M. Bely, I. Masneuf-Pomarede, M. Aigle, and D. Dubourdieu. 2004. Inheritable nature of enological quantitative traits is demonstrated by meiotic segregation of industrial wine yeast strains. FEMS Yeast Reasearch. 4:711-719

• http://www.tactri.gov.tw/htdocs/mllee/Fusarium.html • http://140.130.177.203/jgtsay/jg6-101.html