energy and nutrient utilization responses of broilers fed diets supplemented with an α amylase

RESULTS Table 1. Ileal digestibility and total tract utilization of nutrients and energy of corn or corn- soybean meal diets supplemented with an α-amylase + β-glucanase complex for broiler chickens CONCLUSIONS Corn-soybean meal diets supplemented with an α-amylase-β-glucanase complex had increased AME n and IDE releases by 78 and 105 KNU/kg, respectively, whereas a maximum energy release of corn was estimated with 70 KNU/kg. Abstract #135 Energy and nutrient utilization responses of broilers fed diets supplemented with an α-amylase J.O.B. SORBARA* 1 , S.L. VIEIRA 2 , C. STEFANELLO 2 , G.O SANTIAGO 2 , T.C. SIMOES 2 , N.C. SERAFINI 2 , R.G. HERMES 1 1 DSM Nutritional Products, São Paulo, Brazil; 2 UFRGS Departamento de Zootecnia, Porto Alegre, RS, Brazil INTRODUCTION Exogenous carbohydrases such as xylanases, amylases, and glucanases have been reported to improve energy utilization and performance of broiler chickens (Olukosi et al., 2008; Vieira et al., 2015). These enzymes may improve the access of endogenous enzymes to cell contents due to hydrolysis of cell wall arabinoxylans (Kocher et al., 2003) as well as to augment endogenous amylase in young birds (Gracia et al., 2003). Decreases in endogenous amino acid losses may also contribute to the beneficial effects of amylases (Jiang et al., 2008). OBJECTIVE Evaluate the effects of adding an exogenous α-amylase-β-glucanase complex on energy utilization, nutrient digestibility and total tract retention of corn or corn- soybean meal-based diets in broiler chickens. 1 Crude protein; 2 Ether extract; 3 Linear or quadratic regression effects (P < 0.05). MATERIAL AND METHODS 480 slow feathering Cobb 500 male broilers Experimental diets fed from 15 to 24 d Broilers allocated into 80 battery cages; 10 treatments with 8 replications of 6 birds A Basal diet (Basal) had industry nutrient levels (21.0% CP, 1.15% dig. Lys, 0.86% dig. TSAA, 0.75% dig. Thr and 3,100 kcal AME/kg). A diet having corn replaced at 40% (Test) was also tested Basal and Test diets had Celite as indigestible marker at 1% and were supplemented with 40, 80, 120, and 160 kilo-Novo α-amylase units (KNU) per kg of feed (RONOZYME® A, DSM Nutritional Products) Excreta samples were collected from 21 to 23 d, whereas all birds were slaughtered at 24 d for ileal content collection Data were analyzed using SAS (SAS Institute, 2009) Item CORN-SOYBEAN MEAL DIET CORN Ileal digestibility Total tract retention Ileal digestibili ty Total tract retention DM, % IDE, kcal/k g DM, % AME, kcal/kg AME n , kcal/kg CP 1 , % EE 2 , % DM, % IDE, kcal/ kg DM, % AME, kcal/k g AME n , kcal/k g CP, % EE, % Amylase, KNU/kg 0 61.7 3,016 70.4 3,421 2,934 65.7 83.0 72.3 3,512 77.4 3,561 3,493 52.0 73.1 40 64.8 3,266 75.4 3,563 3,036 68.8 85.6 77.2 3,525 79.9 3,607 3,545 57.0 75.5 80 65.1 3,271 75.9 3,638 3,107 69.2 85.4 79.1 3,772 80.8 3,681 3,597 57.9 74.9 120 65.4 3,278 72.9 3,521 2,955 68.1 85.1 78.8 3,586 81.5 3,653 3,466 58.9 73.4 160 66.0 3,281 71.8 3,505 2,939 68.0 84.6 73.8 3,540 79.2 3,474 3,560 58.0 70.6 SEM 0.469 29.365 0.535 24.364 20.573 0.592 0.755 0.993 42.13 7 0.587 32.111 29.539 0.882 0.749 P-value 3 L 0.004 0.007 0.957 0.473 0.632 0.363 0.642 0.522 0.676 0.181 0.580 0.247 0.098 0.188 Q 0.155 0.027 0.002 0.011 0.007 0.103 0.335 0.008 0.116 0.032 0.040 0.013 0.025 0.065

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Page 1: Energy and nutrient utilization responses of broilers fed diets supplemented with an α amylase

RESULTSTable 1. Ileal digestibility and total tract utilization of nutrients and energy of corn or corn-soybean meal diets supplemented with an α-amylase + β-glucanase complex for broiler chickens

CONCLUSIONSCorn-soybean meal diets supplemented with an α-amylase-β-glucanase complex had increased AMEn and IDE releases by 78 and 105 KNU/kg, respectively, whereas a maximum energy release of corn was estimated with 70 KNU/kg.

Abstract #135

Energy and nutrient utilization responses of broilers fed diets supplemented with an

α-amylaseJ.O.B. SORBARA*1, S.L. VIEIRA2, C. STEFANELLO2, G.O SANTIAGO2, T.C. SIMOES2, N.C. SERAFINI2 , R.G. HERMES1

1DSM Nutritional Products, São Paulo, Brazil; 2UFRGS Departamento de Zootecnia, Porto Alegre, RS, Brazil

INTRODUCTIONExogenous carbohydrases such as xylanases, amylases, and glucanases have been reported to improve energy utilization and performance of broiler chickens (Olukosi et al., 2008; Vieira et al., 2015). These enzymes may improve the access of endogenous enzymes to cell contents due to hydrolysis of cell wall arabinoxylans (Kocher et al., 2003) as well as to augment endogenous amylase in young birds (Gracia et al., 2003). Decreases in endogenous amino acid losses may also contribute to the beneficial effects of amylases (Jiang et al., 2008).

OBJECTIVEEvaluate the effects of adding an exogenous α-amylase-β-glucanase complex on energy utilization, nutrient digestibility and total tract retention of corn or corn-soybean meal-based diets in broiler chickens.

1Crude protein; 2Ether extract; 3Linear or quadratic regression effects (P < 0.05).

MATERIAL AND METHODS480 slow feathering Cobb 500 male broilersExperimental diets fed from 15 to 24 d

Broilers allocated into 80 battery cages; 10 treatments with 8 replications of 6 birds

A Basal diet (Basal) had industry nutrient levels (21.0% CP, 1.15% dig. Lys, 0.86% dig. TSAA, 0.75% dig. Thr and 3,100 kcal AME/kg). A diet having corn replaced at 40% (Test) was also tested

Basal and Test diets had Celite as indigestible marker at 1% and were supplemented with 40, 80, 120, and 160 kilo-Novo α-amylase units (KNU) per kg of feed (RONOZYME® A, DSM Nutritional Products)

Excreta samples were collected from 21 to 23 d, whereas all birds were slaughtered at 24 d for ileal content collection

Data were analyzed using SAS (SAS Institute, 2009)

Item

CORN-SOYBEAN MEAL DIET CORNIleal digestibility Total tract retention Ileal digestibility Total tract retention

DM, % IDE, kcal/kg

DM, % AME,

kcal/kgAMEn, kcal/kg CP1, % EE2, %

DM, % IDE,

kcal/kg

DM, % AME, kcal/kg

AMEn, kcal/kg CP, % EE, %

Amylase, KNU/kg 0 61.7 3,016 70.4 3,421 2,934 65.7 83.0 72.3 3,512 77.4 3,561 3,493 52.0 73.1

40 64.8 3,266 75.4 3,563 3,036 68.8 85.6 77.2 3,525 79.9 3,607 3,545 57.0 75.580 65.1 3,271 75.9 3,638 3,107 69.2 85.4 79.1 3,772 80.8 3,681 3,597 57.9 74.9120 65.4 3,278 72.9 3,521 2,955 68.1 85.1 78.8 3,586 81.5 3,653 3,466 58.9 73.4160 66.0 3,281 71.8 3,505 2,939 68.0 84.6 73.8 3,540 79.2 3,474 3,560 58.0 70.6

SEM 0.469 29.365 0.535 24.364 20.573 0.592 0.755 0.993 42.137 0.587 32.111 29.539 0.882 0.749P-value3 L 0.004 0.007 0.957 0.473 0.632 0.363 0.642 0.522 0.676 0.181 0.580 0.247 0.098 0.188Q 0.155 0.027 0.002 0.011 0.007 0.103 0.335 0.008 0.116 0.032 0.040 0.013 0.025 0.065