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Compd B

Compd C

Compd D

Compd E

Protein A

Protein B

Protein C

Protein E

Protein D

Compd A

Protein C

Unknown Z

Unknown X

Protein syn.

RNA syn.

DNA syn.

cycloheximidereveromycin A

actinomycin Dtoyocamycin

aphidicolindoxorubicinetoposide

Microtubulevinblastinenocodadzoletaxol

Kinesin Eg5

Actinlatrunculincytochalasinjasplakinolide

Metal chelatenigericinmonensin

ARF-GEFbreferdin A

HDACtrichostatin Ascriptaid

ProteasomelactacystinMG132

HSP90geldanamycinradicicol

leptomycin B

terpendole Emonastrol

CRM1

vehicle ICRF193BNS22 TOP2 cat.tsNRK HeLa

1) 2) 3) 4) 5) 6)

11) 12)

7) 8) 9)

10)

12

CellNuclear Granular

INCA2000

PC1

PC2

2.0-0.4-1.5

0.7

Tubulin

Actin

Eg5

DNA syn

TOP2 catalytic

RNA syn

Protein syn

Control

V-ATPase

PP2A

HDAC

HSP90

HSP60ionophore

NPD6689

N

O

OO

O

Chem Biol, 2012

Pyrrolizilactone

N

O

O

O

O

OH

H

H

H

ChemBioChem, 2013

GN39482J Med Chem, 2015

NHN

NHO

O

O

Proteasome

主成分分析

Pyricularia Oryzae

u

u

u 1993

Swelling Clear swelling Black swelling Mixed swelling

Lipopeptin ACystamycin

Polyoxin D PiericidinAntimycin AUK-2A

Monensin

Normal hyphae Short Toxic Beads

Vehicle Miconazole Amphotericin BReveromycin A

Oligomycin ABafilomycin AFolimycin A

P. oryzae 8

Accuracy : 98.7 %