fig. s1. construction of the k. pneumoniae ard -deficient mutant
DESCRIPTION
Journal of Industrial Microbiology and Biotechnology Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high level production of L-2,3-butanediol - PowerPoint PPT PresentationTRANSCRIPT
Fig. S1. Construction of the K. pneumoniae ard-deficient mutant.
X
(Probe apr)
SalI 2.4 kb
ard
aac(3)IV
1.8 kb
ldhA ldhA
(ldhA ard) XldhA
ldhA
Probe ard
Probe apr
(Probe up)
(ldh
A ard
)
1.8 kb
2.4 kb
ldhA
(ldh
A ard
)
SalI
SalISalI
Journal of Industrial Microbiology and Biotechnology
Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high level production of L-2,3-butanediol Jang Min Park Won-Kyung Hong Sung-Mok Lee Sun-Yeon Heo You Ree Jung In Yeong Kang Baek-Rock Oh Jeong-Woo Seo () Chul Ho Kim ()Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South KoreaTel: +82 63 570 5160; Fax: +82 63 570 5109; E-mail: [email protected] (J. W. Seo); Tel: +82 63 570 5110; Fax: +82 63 570 5109; E-mail: [email protected] (C. H. Kim)