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Flash Chromatography Column Guide• Medicinal Chemistry • Natural Products • Proteins & Peptides

www.interchiminc.com

50% Less Solvent

Sample Load 1.5g / 6%25g PuriFlash HC25

68% Less Solvent

Sample Load 3.0g / 12% 25g PuriFlash HC15 Sample Load

1.25g / 3%

Sample Load: 1.0g / 2.5%40g Conventional Flash

Partial Purity

Solvent savings and higher loading based on purifying the equivalent amount of sample on a conventional flash column.

100% Purity

PuriFlash HC Silica Columns

40g Conventional 1.0 g 11 263 ml 480 ml25g PuriFlash HC50 1.0 g 7 144 ml 340 ml25g PuriFlash HC25 1.5 g 9 199 ml 360 ml25g PuriFlash HC15 3.0 g 17 367 ml 460 ml

Sample Load

Collected Volume

Solvent ConsumedTubesColumn

Test ConditionsSample: 500 mg/ml dibutyl and diethyl phthalateFlow rate: 20 ml/minSolvents: A: Heptane B: EtOAcGradient: 0 - 7 CV 5% B, 7 - 13 CV 5 - 8% B

UV: 254 nmTube volume: 25 ml

% Loading: Sample / silica (w/w)

Partial Purity / Overload Condition

Sample Load

Collected Volume

Solvent ConsumedTubes

Column

40g Conventional 1.25 g 13 298 ml 540 ml 25g PuriFlash HC50 1.25 g 8 186 ml 360 ml

Sample Load: 1.0g / 4% 25g PuriFlash HC50

Partial Purity

30% Less Solvent

Sample Load1.25g / 5%

Interchim uses spherical shaped particles instead of conventional flash media (irregular shaped 40-63u). Spherical silica provides 500% higher loading with 70% less solvent and reduces collection volume by 300%

PuriFlash HC columns contain a new advanced silica technology based on ultra-high surface area spherical silica. In this example a 25 gram puriFlash column purified the same amount of sample loaded on a conventional 40 gram column. For higher loading and increased resolution the 25µ and 15µ columns are recommended. The smaller particle sizes increase resolution and provide up to 5 times the loading compared to conventional flash columns

Conventional flash columns contain irregular shaped particles

PuriFlash columns contain spherical silica • 500% higher loading • 70% less solvent • 300% less collection volume

50µ25µ

15µ

1Flash Chromatography Column GuideMedicinal Chemistry • Natural Products

• Proteins & Peptides

Normal Phase Jumbo Packs 23

Reverse Phase, Linear Scale-up from Analytical to Flash

24

Column Hardware Physical Specifications 25

Column Loading Chart Back Cover

Alumina — ALN: Neutral, ALB: Basic 10

Dry Load — Empty or Partial Fill: Celite, Silica, C18 11

Carbon Columns for Decolorization 10

Ion Exchange — SCX, SAX, MM1 14

ColumnsSilica — HC: High Capacity, HP: High Performance 8, 23

Reverse Phase — C18HC, C18HP, C18HQ 15, 16

Reverse Phase High Aqueous — RPAQ, C18AQ 17

Reverse Phase Polymers — Atoll X, 302H 16

Cyano, Diol, HILIC, DEAP, NH2 12, 13

Cellufine — Ion Exchange, Affinity, HIC, Endotoxin Removal 21, 22

Reverse Phase Bonded Silica— C4, C8, C18 (200 & 300Å) 18 - 20

Biopurification

Natural Products — P6 14

General Guidelines

Column Selection Chart

2

2

Column Selection Guide 3

Column Selection Guidelines

TLC Screening for Flash Optimization 7

2 Column Selection Chart

Sample Column Selection

Charged

Acidic

Basic

Low to MidPolarity

Acid Sensitive

HighPolarity

Reverse PhaseC18HP, C18HC

C18HQ, CN

Reverse PhaseC18HP, C18HCC18HQ, CN, P6

Reverse PhaseRPAQ, Diol, 302H,

NH2HC, DEAP

Reverse PhaseC18HP, C18HC

C18HQ, CN, NH2HCALB, MM1, DEAP

Reverse PhaseC18AQ, C18HP, X

C18HC, C18HQ, CN, NH2HC, MM1, DEAP

Normal PhaseDiol, CN, ALN

Normal PhaseHILIC

Normal PhaseSIHC, SIHP, ALN

Normal PhaseDiol, CN

Normal PhaseSIHC, SIHP, ALN,ALB, CN, NH2HC,

DEAP

Normal PhaseSIHC, SIHP, ALN,

Diol, HILIC

Reverse PhaseDiol

Ion ExchangeSCX, MM1,

NH2HC, DEAP

Ion ExchangeSAX, NH2HC,

DEAP

Ion ExchangeMM1

Normal Phase: In normal-phase chromatography, the stationary phase is polar and the mobile phase is less polar. The more polar the solvent, the greater the elution strength. To determine optimum conditions, please refer to TLC screening on page 7. Includes: SIHC, SIHP, ALB, ALN, NH2HC, NH2, DEAP, HILIC, DIOL, CN

Use: It is possible to re-use puriFlash HC & HP columns by flushing with 100% methanol after each run and then completely remove the methanol by purging with air.Storage: Store dry after purging with methanol and air. Do not use hexane as it will soften the polypropylene column hardware.

Reverse Phase: In reverse phase chromatography, the stationary phase is non-polar or weakly polar and the mobile phase is more polar. A less polar solvent has higher elution strength. Combinations of water with either methanol or acetonitrile are most often used for gradient elution. Compared to normal phase, reverse phase provides higher efficiency and improved selectivity but lower sample loading capacity. Includes: C18HC, C18HP, C18HQ, C18AQ, RPAQ, 302H, X, CN, NH2HC, NH2, MM1, ALB, P6, DEAP

Use: It is possible to re-use puriFlash reverse phase columns by flushing with 100% organic after each run.Storage: Remove organic modifiers, buffer salts and flush with 75% Methanol or Acetonitrile in water with end caps in place.

Ion Exchange: In ion exchange chromatography, the stationary phase is bonded with ionic functional groups that retain the solute ions of opposite charge. Includes: SCX, SAX, MM1, NH2HC, NH2, DEAP

To Retain: Anion Exchange - pKa of the target compound < pH < pKa of the sorbent Cation Exchange - pKa of the sorbent < pH < pKa of the target compoundTo Elute: Anion Exchange - pKa of the sorbent < pH < pKa of the target compound Cation Exchange - pKa of the target compound < pH < pKa of the sorbent Storage: Remove buffer salts by flushing with water and store in 100% Acetonitrile with end caps in place.

General Guidelines

3PuriFlash Column Selection Guide

Type Application Capacity Base MaterialpH

StabilityParticleSizes Page

NH2HC Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. High Capacity, for higher loading. Eliminate the use of TEA (triethylamine) for basic compounds.

7% High Capacity Spherical Silica 1.5 - 6.5 50µ

NH2 Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. Eliminate the use of TEA (triethylamine) for basic compounds.

5% High Performance Spherical Silica 2 - 6.5 30 & 50µ

HILIC Very polar water soluble compounds; dyes, polyphenols, nucleotides and alkaloids using aqueous solvent systems. Reduce the use of chlorinated solvents using 100% MeOH or H2O. Reduced rota-vap time by using less water.

7% High Performance Spherical Silica 1.5 - 7.5 30µ

DIOL Enhanced selectivity and higher retention for low to medium polarity compounds vs. bare silica. 6% High Performance

Spherical Silica 1.5 - 7 30 & 50µ

CNMid range polarity between bare silica and C18. 6% High Performance

Spherical Silica 1.5 - 7 50µ

DEAP DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water for use in normal or reverse phase.

Granular Silica 60µ

Normal Phase, Bonded Silica

Page 1 of 1

Normal Phase, Bonded Silica



SIHC Non-ionic polar organic compounds. Methanol stable, high loading capacity and high resolution. 20% High Capacity

Spherical Silica 1.5 - 6.5 15, 25 & 50µ

SIHP Non-ionic polar organic compounds. Methanol stable, high separation efficiency at high flow rates. 10% High Performance

Spherical Silica 1.5 - 6.5 15, 30 & 50µ

ALB Basic compound purification. Eliminate the use of TEA (triethylamine) to avoid streaking or tailing under normal or reverse phase conditions.

10% Granular Basic Alumina 1 - 12 45µ

ALN Acid sensitive compounds that would degrade if exposed to normal phase silica. 10% Granular Neutral

Alumina 1 -12 45µ

Dry-Load Hand tight, no adapters, stable to 15 bar / 215 PSI NA Silica or Celite 1.5 - 6.5 50µ Carbon Decolorization, no exposure to carbon dust 10% Activated Carbon 1 - 14 50µ

Normal Phase, Bare Silica Alumina and Dry-Load

Page 1 of 1

Normal Phase: Bare Silica, Alumina, Dry-Load and Decolorization

O Si OH

HC, HP Silica

O

Al

AlO

O Na

Na

Alumina Basic

Al

O

Al

O

Alumina Nuetral

O

O

OH

Si

Si

CH3

(CH2)3

CH3

HH

CH3

N

NH2 & NH2 HC

O

O

OH

Si

Si

CH3

(CH2)3

CH3

C

CH3

N

CN HILIC

O

OH

Si (Cx)x

+O O

OH

OH OH

OH

Si (CH2)3 CH2 CH2CH

Diol

8

9

10

10

11

14

13

13

12

12

12

13

4



C18HPNon polar and ionic compounds. Fully end-capped. 3% High Performance

Spherical Silica 1 - 7.5 15, 30 & 50µ

C18HC Non polar and ionic compounds. Fully end-capped. 3% High Capacity Spherical Silica 1 - 7.5 15 & 30µ

C18HQNon polar and ionic compounds. Fully end-capped. 3%

HPLC Prep Grade, Spherical

Silica1 - 7.5 15µ

C18AQ Mid polar compounds. Start gradient with 100% water. 3% High Performance

Spherical Silica 1.5 - 7.5 15 & 30µ

RPAQPolar compounds. Start gradient with 100% water. 3% High Performance

Spherical Silica 1.5 - 7.5 15 & 30µ



302H Hydrophilic/hydrophobic polymer for polar and non polar compounds. MW <3 kDa. High capacity reverse phase.

>25% SDVB Polymer 1 -13 30 & 70µ

Atoll X Atoll X is a universal polymer for mid & non polar compounds, MW <3 kDa. High capacity reverse phase.

>20% SDVB Polymer 1 -13 40µ

P6 Amide functionality for aromatic and natural products; flavonoids, chalkones, anthraquinones, aromatic nitro compounds, DNP amino acids, phenols, carbonic acids, acid amides, sulphonic acids and amides of sulphonic acids and amines.

5% Polyamide 1 - 8 50µ



Reverse Phase, for small molecules

Page 1 of 1

Reverse Phase Bonded Silica and Polymers

O

OH

O

Si (CH2)3 (CH2CH3)2NH+

DEAP

PuriFlash Column Selection Guide

C18HP, C18HC, C18HQ,

O

O

OH

Si CH3

CH3

CH3

Si C18H37

CH3

CH3

C18AQ

O

O

OH

P

Si C18H37

CH3

CH3

RPAQ

O P

O

OH

Si CnHn2+1

CH3

CH3

Atoll X

CH2 CH2

CH2

CH2

CH2

CH

CH

CH

C

OO

OO

302H

CH2

x y

CHP

O

P6

(CH2)5

n

C

O

HN

The following phases can also be used for reverse phase: CN, NH2, ALB (page 3) and MM1 (page 5)

15

15

16

17

17

13

16

16

14

4%

5



SCX Sulfonic acid functionality which retains weak basic compounds that have one or more positive charges. Remove or isolate basic compounds.

0.3 meq/g High Performance Spherical Silica 1 - 7.5 50µ

SAX Quaternary amine functionality which retains strong (pKa <1) or weak acids (pKa >2). 0.3 meq/g Granular Silica 1.5 - 7 50µ

MM1 Unique selectivity of basic, charged and non-polar compounds via ion exchange and hydrophobic chains (SCX/C8). Compounds that possess basic functionality are retained by ion exchange. A washing step with an appropriate pH, elutes ionizable compounds. Retained compounds from hydrophobic bonding are eluted with an organic solvent.

0.09 meq/g High Performance Spherical Silica 1.0 - 7.5 60µ

NH2HC Weak anion exchange for strong acids 0.3 meq/g High Capacity Spherical Silica 1.5 - 6.5 50µ

NH2Weak anion exchange for strong acids 0.3 meq/g High Performance

Spherical Silica 2 - 6.5 30 & 50µ

DEAP Weak anion exchange (secondary amino, pKa 10.5) 0.3 meq/g Granular Silica 60µ

Ion Exchange, Bonded Silica

Page 1 of 1

Ion Exchange, Bonded Silica

O

O

O

O-H+S

OH

OH

Si (CH2)3 O

SCX

O

OH

OH

Si N+(CH2)3 (CH3)3

SAX


O

O

O

O-H+S

OH

OH

Si (CH2)3 O

MM1

Si CnHn2+1

CH3

CH3

14

13

14

14

13

13

O

O

OH

Si

Si

CH3

(CH2)3

CH3

HH

CH3

N

NH2 & NH2 HC

O

OH

O

Si (CH2)3 (CH2CH3)2NH+

DEAP

6



90A500(DEAE) Weak anion exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa.>60 mg/ml

BSA Cellulose Beads 1 - 13 90µ

90Q500(QA) Strong anion exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa. >10 mg/ml

BSA Cellulose Beads 2 - 12 90µ

90C500(CM) Weak cation exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa. >70 mg/ml Lysozyme Cellulose Beads 1 - 13 90µ

75SO4(Sulfate) Affinity purification of Virus and heparin binding

protein up to 3 kDa. >3 mg/ml Lysozyme Cellulose Beads 3 - 12 75µ

90EL Affinity, endotoxin removal up to 2000 kDa. 480 µg/ml Cellulose Beads 2 - 13 90µ75ES Affinity, endotoxin removal up to 2 kDa. 185 µg/ml Cellulose Beads 2 - 13 75µ

90C4(butyl)

Hydrophobic interaction for hydrophobic proteins up to4000 kDa

25 mg/ml BSA Cellulose Beads 1 - 13 90µ

90PH(phenyl)

Hydrophobic interaction for hydrophobic proteins and enzymes up to 4000 kDa

30 mg/ml BSA Cellulose Beads 1 - 13 90µ

Cellulose media for biopurification

Page 1 of 1

Cellulose Media for Biopurification



PP-C18T Moderately hydrophobic peptides & oligopeptides.<40 kDa 2% 300Å Spherical

Silica 1.5 - 8 15 & 30µ

PT-C18T Weak hydrophobic peptides/oligopeptides. <50 kDa 2% 200Å Spherical Silica 1.5 - 8 15 & 30µ

PP-C8 Hydrophobic polypeptides & peptides. <80 kDa 2% 300Å Spherical Silica 2 - 7 15 & 30µ

PT-C8 Moderately hydrophobic peptides & oligopeptides.<60 kDa 2% 200Å Spherical

Silica 2 - 7 15 & 30µ

PP-C4 Hydrophobic proteins & polypeptides 50 - 150 kDa 2% 300Å Spherical Silica 2 - 7 15 & 30µ

PT-C4 Hydrophobic peptides & oligopeptides. <60 kDa 2% 200Å Spherical Silica 2 - 7 15 & 30µ

Reverse Phase, for macro-molecules

Page 1 of 1

Reverse Phase for Biopurification


C18T

O OOH

SiCH

3

CH3

CH3

SiC 18

H37

R R

O OOH

SiCH

3

CH3

CH3

SiC 8H

17

R R

C8 Bio

O OOH

SiCH

3

CH3

CH3

SiC 4H

9

R R

C4 Bio

18

21

18

21

19

21

20

21

22

20

22

19

22

22

7TLC Screening for Flash Optimization

Prior to running a sample on a flash instrument/column it is important to determine the optimum solubility, selectivity and resolution by TLC. The following solvent systems provide a wide range of selectivities and are a useful screening tool. Valuable information is obtained by running the sample in several mobile phases and will improve the odds of obtaining purity using Flash. It is also useful to create a weak and strong polarity for each of the mobile phases to obtain additional data points.

The recommended TLC plate which best correlates to puriFlash HC & HP Silica columns is provided by EM Merck, Part number 15341-1, 2.5 x 7.5cm.

Solvent System

Compound Characteristics TLC conditions Comments

EtOAc:Hexanes

DCM:MeOH + NH3

Diethyl ether: Hexanes or THF

Lipophilic molecules without ionizable groups

Molecules with basic amine functionality

Non-polar molecules

Mixture applied to flash column should be soluble or partially soluble in the mobile phase. Adding DCM will aid solubility but reduce retention.

It is common for streaking to oc-cur with basic amines. To reduce streaking/band broadening add an amine modifier (a few drops of NH3 or a solution of NH3 in MeOH). Adding an amine modifier can improve compound recovery from the column.

Use methyl tetrahydrofuran instead of THF which does not readily form peroxides.

Start at 1:1 and adjust until Rf of target is 0.2 - 0.3

90:10 DCM:MeOH and adjust MeOH until Rf of target is 0.2 - 0.3

Start at 1:1 and adjust until Rf of target is 0.2 - 0.5

DCM:MeOH

DCM:Hexanes: MeOH + NH3

Lipophilic molecules without ionizable groups

Molecules with basic amine functionality

If you have an ester functional group in your product, transesteri-fication is possible. This happens when the product ester is ex-changed with the solvent alcohol used in the mobile phase - if you have an ethyl ester in your product and you use MeOH in the mobile phase you may end up with a small amount of methyl ester in your product. Silica can be acidic and will promote this type of reaction.

If you need to use DCM:MeOH but it is still too polar you can add hexanes (or replace the DCM with hexanes). This will improve the Rf without changing the nature of the column and retain the solubilizing power of the mobile phase.

If above conditions do not move target at 100% EtOAC, add MeOH to DCM at 1% up to 15%.

90:10 DCM MeOH and adjust Hexanes until Rf of target is 0.2 - 0.3

8

Part number Description Price UnitPF-15SIHCKA PuriFlash Column Kit,15µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $325 KITPF-15SIHCKB PuriFlash Column Kit,15u Si HC, 1 EACH;80g, 120g, 200g, 300g $625 KITPF-15SIHCKC PuriFlash Column Kit,15µ Si HC, 2 EACH; 4G, 12G, 25G, 40G $165 KIT

PF-25SIHCKA PuriFlash Column Kit, 25µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $195 KITPF-25SIHCKB PuriFlash Column Kit, 25µ Si HC, 2 EACH; 80G, 120G, 200G, 300G $675 KITPF-25SIHCKC PuriFlash Column Kit, 25µ Si HC, 1 EACH; 80G, 120G, 200G, 300G $350 KIT

PF-50SIHCKA PuriFlash Column Kit, 50µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $135 KITPF-50SIHCKB PuriFlash Column Kit, 50µ Si HC 2 EACH; 80G, 120G, 200G, 300G $395 KIT

PF-15SIHC/4G PuriFlash Column, 15µ Si HC, 4G $220 20/PKPF-15SIHC/12G PuriFlash Column, 15µ Si HC, 12G $530 20/PKPF-15SIHC/25G PuriFlash Column, 15µ Si HC, 25G $485 12/PKPF-15SIHC/40G PuriFlash Column, 15µ Si HC, 40G $715 12/PKPF-15SIHC/80G PuriFlash Column, 15µ Si HC, 80G $505 4/PKPF-15SIHC/120G PuriFlash Column, 15µ Si HC, 120g $725 4/PKPF-15SIHC/200G PuriFlash Column, 15µ Si HC, 200g $620 2/PKPF-15SIHC/300G PuriFlash Column, 15µ Si HC, 300g $775 2/PKPF-15SIHC/800G PuriFlash Column, 15µ Si HC, 800g $1,225 EAPF-15SIHC/1600G PuriFlash Column, 15µ Si HC, 1600g $2,350 EA

PF-25SIHC/4G PuriFlash Column, 25µ Si HC, 4g $370 40/PKPF-25SIHC/12G PuriFlash Column, 25µ Si HC, 12g $385 30/PKPF-25SIHC/25G PuriFlash Column, 25µ Si HC, 25g $495 25/PKPF-25SIHC/40G PuriFlash Column, 25µ Si HC, 40g $530 20/PKPF-25SIHC/80G PuriFlash Column, 25µ Si HC, 80g $415 10/PKPF-25SIHC/120G PuriFlash Column, 25µ Si HC, 120g $450 8/PKPF-25SIHC/200G PuriFlash Column, 25µ Si HC, 200g $400 4/PKPF-25SIHC/300G PuriFlash Column, 25µ Si HC, 300g $635 4/PKPF-25SIHC/800G PuriFlash Column, 25µ Si HC, 800g $445 EAPF-25SIHC/1600G PuriFlash Column, 25µ Si HC, 1600g $785 EA

PF-50SIHC/4G PuriFlash Column, 50µ Si HC, 4g $275 40/PKPF-50SIHC/12G PuriFlash Column, 50µ Si HC, 12g $305 30/PKPF-50SIHC/25G PuriFlash Column, 50µ Si HC, 25g $320 25/PKPF-50SIHC/40G PuriFlash Column, 50µ Si HC, 40g $320 20/PKPF-50SIHC/80G PuriFlash Column, 50µ Si HC, 80g $335 10/PKPF-50SIHC/120G PuriFlash Column, 50µ Si HC, 120g $370 8/PKPF-50SIHC/200G PuriFlash Column, 50µ Si HC, 200g $310 4/PKPF-50SIHC/300G PuriFlash Column, 50µ Si HC, 300g $425 4/PKPF-50SIHC/800G PuriFlash Column, 50µ Si HC, 800g $260 EAPF-50SIHC/1600G PuriFlash Column, 50µ Si HC, 1600g $530 EAPF-50SIHC-KG PuriFlash 50µ Si HC Bulk $160 KG

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PuriFlash Normal Phase HC: High Capacity Silica

9

Part number Description Price UnitPF-15SIHPKA PuriFlash Column Kit, 15µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $475 KITPF-15SIHPKB PuriFlash Column Kit, 15µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $985 KITPF-15SIHPKC PuriFlash Column Kit, 15µ Si HP, 2 EACH; 4G, 12G, 25G, 40G $240 KIT

PF-30SIHPKA PuriFlash Column Kit, 30µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $195 KITPF-30SIHPKB PuriFlash Column Kit, 30µ Si HP, 2 EACH; 80G, 120G, 200G, 300G $675 KITPF-30SIHPKC PuriFlash Column Kit, 30µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $340 KIT

PF-50SIHPKA PuriFlash Column Kit, 50µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $145 KITPF-50SIHPKB PuriFlash Column Kit, 50µ Si HP 2 EACH; 80G, 120G, 200G, 300G $460 KIT

PF-15SIHP/4G PuriFlash Column, 15µ Si HP, 4g $220 20/PKPF-15SIHP/12G PuriFlash Column, 15µ Si HP, 12g $530 20/PKPF-15SIHP/25G PuriFlash Column, 15µ Si HP, 25g $485 12/PKPF-15SIHP/40G PuriFlash Column, 15µ Si HP, 40g $715 12/PKPF-15SIHP/80G PuriFlash Column, 15µ Si HP, 80g $505 4/PKPF-15SIHP/120G PuriFlash Column, 15µ Si HP, 120g $725 4/PKPF-15SIHP/200G PuriFlash Column, 15µ Si HP, 200g $620 2/PKPF-15SIHP/300G PuriFlash Column, 15µ Si HP, 300g $775 2/PKPF-15SIHP/800G PuriFlash Column, 15µ Si HP, 800g $1,225 EAPF-15SIHP/1600G PuriFlash Column, 15µ Si HP, 1600g $2,350 EA

PF-30SIHP/4G PuriFlash Column, 30µ Si HP, 4g $370 40/PKPF-30SIHP/12G PuriFlash Column, 30µ Si HP, 12g $385 30/PKPF-30SIHP/25G PuriFlash Column, 30µ Si HP, 25g $495 25/PKPF-30SIHP/40G PuriFlash Column, 30µ Si HP, 40g $530 20/PKPF-30SIHP/80G PuriFlash Column, 30µ Si HP, 80g $415 10/PKPF-30SIHP/120G PuriFlash Column, 30µ Si HP, 120g $450 8/PKPF-30SIHP/200G PuriFlash Column, 30µ Si HP, 200g $400 4/PKPF-30SIHP/300G PuriFlash Column, 30µ Si HP, 300g $635 4/PKPF-30SIHP/800G PuriFlash Column, 30µ Si HP, 800g $445 EAPF-30SIHP/1600G PuriFlash Column, 30µ Si HP, 1600g $785 EA

PF-50SIHP/4G PuriFlash Column, 50µ Si HP, 4g $265 40/PKPF-50SIHP/12G PuriFlash Column, 50µ Si HP, 12g $295 30/PKPF-50SIHP/25G PuriFlash Column, 50µ Si HP, 25g $310 25/PKPF-50SIHP/40G PuriFlash Column, 50µ Si HP, 40g $310 20/PKPF-50SIHP/80G PuriFlash Column, 50µ Si HP, 80g $325 10/PKPF-50SIHP/120G PuriFlash Column, 50µ Si HP, 120g $360 8/PKPF-50SIHP/200G PuriFlash Column, 50µ Si HP, 200g $275 4/PKPF-50SIHP/300G PuriFlash Column, 50µ Si HP, 300g $415 4/PKPF-50SIHP/800G PuriFlash Column, 50µ Si HP, 800g $250 EAPF-50SIHP/1600G PuriFlash Column, 50µ Si HP, 1600g $390 EA

Page 1 of 1

PuriFlash Normal Phase HP: High Performance Silica

10

Part number Description Price UnitPF-ALB/7G PuriFlash Column, 50µ Al Basic, 7g $60 8/PKPF-ALB/25G PuriFlash Column, 50µ Al Basic, 25g $45 4/PKPF-ALB/40G PuriFlash Column, 50µ Al Basic, 40g $60 4/PKPF-ALB/65G PuriFlash Column, 50µ Al Basic, 65g $80 4/PKPF-ALB/135G PuriFlash Column, 50µ Al Basic, 135g $82 2/PKPF-ALB/200G PuriFlash Column, 50µ Al Basic, 200g $115 2/PKPF-ALB/355G PuriFlash Column, 50µ Al Basic, 355g $183 2/PKPF-ALB/550G PuriFlash Column, 50µ Al Basic, 550g $138 EAPF-ALB/1445G PuriFlash Column, 50µ Al Basic, 1445g $342 EAPF-ALB/2900G PuriFlash Column, 50µ Al Basic, 2900g $568 EA

PF-ALN/7G PuriFlash Column, 50µ Al Neutral, 7g $59 8/PKPF-ALN/25G PuriFlash Column, 50µ Al Neutral, 25g $56 4/PKPF-ALN/40G PuriFlash Column, 50µ Al Neutral, 40g $77 4/PKPF-ALN/65G PuriFlash Column, 50µ Al Neutral, 65g $106 4/PKPF-ALN/135G PuriFlash Column, 50µ Al Neutral, 135g $109 2/PKPF-ALN/200G PuriFlash Column, 50µ Al Neutral, 200g $156 2/PKPF-ALN/355G PuriFlash Column, 50µ Al Neutral, 355g $253 2/PKPF-ALN/550G PuriFlash Column, 50µ Al Neutral, 550g $194 EAPF-ALN/1445G PuriFlash Column, 50µ Al Neutral, 1445g $483 EAPF-ALN/2900G PuriFlash Column, 50µ Al Neutral, 2900g $857 EA

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PuriFlash AluminaBasic & Neutral

Part number Description Price UnitPF-AC/3G puriFlash Carbon 3g, 12 x 64 mm (PF-CARB/4G) $105 16PKPF-AC/10G puriFlash Carbon 10g, 21 x 83 mm (PF-CARB/12G) $95 8PKPF-AC/18G puriFlash Carbon 18g, 21 x 133 mm (PF-CARB/25G) $125 8PKPF-AC/30G puriFlash Carbon 30g, 27 x 133 mm (PF-CARB/40G) $165 8PKPF-AC/60G puriFlash Carbon 60g, 31 x 205 mm (PF-CARB/80G) $190 4PKPF-AC/90G puriFlash Carbon 90g, 36 x 223 mm (PF-CARB/120G) $255 4PKPF-AC/160G puriFlash Carbon 160g, 60 x 161 mm (PF-CARB/200G) $405 4PKPF-AC/250G puriFlash Carbon 250g, 60 x 236 mm (PF-CARB/300G) $310 2PKPF-AC/650G puriFlash Carbon 650g, 78 x 345 mm $420 EAPF-AC/1300G puriFlash Carbon 1300g, 105 x 390 mm $695 EA

Page 1 of 1

PuriFlash Carbon ColumnsDe-Colorization

11

Part number Description Price UnitPF-DLE/4G PuriFlash Dryload Column, Empty 7 ml, 12 x 65 mm (FO8880) $80 20/PKPF-DLE/12G PuriFlash Dryload Column, Empty 30 ml, 21 x 80 mm (FO8890) $95 20/PKPF-DLE/25G PuriFlash Dryload Column, Empty 75 ml, 27 x 130 mm (FL9521) $125 20/PKPF-DLE/40G PuriFlash Dryload Column, Empty 45 ml, 21 x 130 mm (FO8900) $135 20/PK

PF-DLSIHC08/4G PuriFlash Dryload Column, HC Silica 3g, 7 ml, 12 x 65 mm $95 20/PKPF-DLSIHC08/12G PuriFlash Dryload Column, HC Silica 11g, 30 ml, 21 x 80 mm $140 20/PKPF-DLSIHC05/25G PuriFlash Dryload Column, HC Silica 20g, 45 ml, 21 x 130mm $340 20/PKPF-DLSIHC05/40G PuriFlash Dryload Column, HC Silica 30g, 75 ml, 27 x 130 mm $195 20/PKPF-50SIHC-CET/200G puriFlash Sample loading column, 200G, 50/50 HC/Celite $125 EA

PF-DLCET08/4G PuriFlash Dryload Column, Celite 2g, 7 ml, 12 x 65 mm $95 20/PKPF-DLCET08/12G PuriFlash Dryload Column, Celite 5g, 30 ml, 21 x 80 mm $140 20/PKPF-DLCET08/25G PuriFlash Dryload Column, Celite 10g, 45 ml, 21 x 130mm $180 20/PKPF-DLCET08/40G PuriFlash Dryload Column, Celite 15g, 75 ml, 27 x 130 mm $210 20/PK

PF-DLSIHC08/25G PURIFLASH-DRY-LOAD SILICA-HC-80% 25G FLASH-COLUMN $157 20/PKPF-DLSIHC08/40G PURIFLASH-DRY-LOAD SILICA-HC-80% 40G FLASH-COLUMN $189 20/PKPF-DLSIHC05/4G PURIFLASH-DRY-LOAD SILICA-HC-50% 4G FLASH-COLUMN $76 20/PKPF-DLSIHC0512G PURIFLASH-DRY-LOAD SILICA-HC-50% 12G FLASH-COLUMN $108 20/PKPF-DLSIHC0525G PURIFLASH-DRY-LOAD SILICA-HC-50% 25G FLASH-COLUMN $143 20/PK

PF-DLIRC808/4G PURIFLASH-DRY-LOAD C18-STD-80% 4G FLASH-COLUMN $51 5/PKPF-DLIRC1808/12G PURIFLASH-DRY-LOAD C18-STD-80% 12G FLASH-COLUMN $146 5/PKPF-DLIRC1808/25G PURIFLASH-DRY-LOAD C18-STD-80% 25G FLASH-COLUMN $232 5/PKPF-DLIRC1808/40G PURIFLASH-DRY-LOAD C18-STD-80% 40G FLASH-COLUMN $348 5/PK

PF-DLIRC1805/4G PURIFLASH-DRY-LOAD C18-STD-50% 4G FLASH-COLUMN $43 5/PKPF-DLIRC180512G PURIFLASH-DRY-LOAD C18-STD-50% 12G FLASH-COLUMN $105 5/PKPF-DLIRC1805/40G PURIFLASH-DRY-LOAD C18-STD-50% 40G FLASH-COLUMN $240 5/PK

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PuriFlash Dry Load ColumnsEmpty or partially filled with Silica, Celite or C18

Prep HPLC, Spot II50 ml/min, 4350 PSI200 ml/min, 3300 PSI500 ml/min, 1600 PSI24" x 23" x 26"

Flash, Evo Series150 ml/min, 150 PSI200 ml/min, 435 PSI250 ml/min, 750 PSI 800 ml/min, 150 PSI19" x 17" x 26"

HPLC pumps for reliabilityDetection with UV, ELSD

Same software for Flash & HPLC

PURIFICATION INSTRUMENTATION FOR ALLInterchim offers the most comprehensive range of instruments from Flash to preparative HPLC

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Part number Description Price UnitPF-30DIOL/6G PuriFlash Column, 30µ Diol, 6g $290 4/PKPF-30DIOL/20G PuriFlash Column, 30µ Diol, 20g $285 2/PKPF-30DIOL/35G PuriFlash Column, 30µ Diol, 35g $160 EAPF-30DIOL/55G PuriFlash Column, 30µ Diol, 55g $200 EAPF-30DIOL/120G PuriFlash Column, 30µ Diol, 120g $390 EAPF-30DIOL/175G PuriFlash Column, 30µ Diol, 175g $575 EAPF-30DIOL/300G PuriFlash Column, 30µ Diol, 300g $1,075 EAPF-30DIOL/450G PuriFlash Column, 30µ Diol, 450g $1,655 EAPF-30DIOL/1250G PuriFlash Column, 30µ Diol, 1250g $3,410 EAPF-30DIOL/2500G PuriFlash Column, 30µ Diol, 2500g $6,825 EA

PF-50DIOL/6G PuriFlash Column, 50µ Diol, 6g $75 4/PKPF-50DIOL/20G PuriFlash Column, 50µ Diol, 20g $109 2/PKPF-50DIOL/35G PuriFlash Column, 50µ Diol, 35g $85 EAPF-50DIOL/55G PuriFlash Column, 50µ Diol, 55g $130 EAPF-50DIOL/120G PuriFlash Column, 50µ Diol, 120g $277 EAPF-50DIOL/175G PuriFlash Column, 50µ Diol, 175g $406 EAPF-50DIOL/300G PuriFlash Column, 50µ Diol, 300g $701 EAPF-50DIOL/450G PuriFlash Column, 50µ Diol, 450g $1,081 EAPF-50DIOL/1250G PuriFlash Column, 50µ Diol, 1250g $2,818 EAPF-50DIOL/2500G PuriFlash Column, 50µ Diol, 2500g $5,537 EA

PF-30HILIC/6G PuriFlash Column, 30µ Hilic, 6g $78 4/PKPF-30HILIC/20G PuriFlash Column, 30µ Hilic, 20g $122 2/PKPF-30HILIC/35G PuriFlash Column, 30µ Hilic, 35g $95 EAPF-30HILIC/55G PuriFlash Column, 30µ Hilic, 55g $149 EAPF-30HILIC/120G PuriFlash Column, 30µ Hilic, 120g $319 EAPF-30HILIC/175G PuriFlash Column, 30µ Hilic, 175g $467 EAPF-30HILIC/300G PuriFlash Column, 30µ Hilic, 300g $813 EAPF-30HILIC/450G PuriFlash Column, 30µ Hilic, 450g $1,250 EAPF-30HILIC/1250G PuriFlash Column, 30µ Hilic, 1250g $3,289 EAPF-30HILIC/2500G PuriFlash Column, 30µ Hilic, 2500g $6,488 EA

PF-50CN/6G PuriFlash Column, 50µ CN 6G $89 4PKPF-50CN/20G PuriFlash Column, 50µ CN 20G $138 2PKPF-50CN/35G PuriFlash Column, 50µ CN 35G $111 EAPF-50CN/55G PuriFlash Column, 50µ CN 55G $173 EAPF-50CN/120G PuriFlash Column, 50µ CN 120G $370 EAPF-50CN/175G PuriFlash Column, 50µ CN 175G $543 EAPF-50CN/300G PuriFlash Column, 50µ CN 300G $945 EAPF-50CN/450G PuriFlash Column, 50µ CN, 450g $1,455 EAPF-50CN/1250G PuriFlash Column, 50µ CN, 1250g $3,823 EAPF-50CN/2500G PuriFlash Column, 50µ CN, 2500g $7,563 EA

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PuriFlash Normal PhaseDiol, HILIC & CN Bonded Silica

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Part number Description Price UnitPF-30NH2/6G PuriFlash Column, 30µ NH2, 6g $280 4/PKPF-30NH2/20G PuriFlash Column, 30µ NH2, 20g $275 2/PKPF-30NH2/35G PuriFlash Column, 30µ NH2, 35g $165 EAPF-30NH2/55G PuriFlash Column, 30µ NH2, 55g $195 EAPF-30NH2/120G PuriFlash Column, 30µ NH2, 120g $409 EAPF-30NH2/175G PuriFlash Column, 30µ NH2, 175g $598 EAPF-30NH2/300G PuriFlash Column, 30µ NH2, 300g $1,037 EAPF-30NH2/450G PuriFlash Column, 30µ NH2, 450g $1,602 EAPF-30NH2/1250G PuriFlash Column, 30µ NH2, 1250g $4,185 EAPF-30NH2/2500G PuriFlash Column, 30µ NH2, 2500g $8,281 EA

PF-50NH2/6G PuriFlash Column, 50µ NH2, 6g $95 4/PKPF-50NH2/20G PuriFlash Column, 50µ NH2, 20g $125 2/PKPF-50NH2/35G PuriFlash Column, 50µ NH2, 35g $100 EAPF-50NH2/55G PuriFlash Column, 50µ NH2, 55g $150 EAPF-50NH2/120G PuriFlash Column, 50µ NH2, 120g $320 EAPF-50NH2/175G PuriFlash Column, 50µ NH2, 175g $455 EAPF-50NH2/300G PuriFlash Column, 50µ NH2, 300g $795 EAPF-50NH2/450G PuriFlash Column, 50µ NH2, 450g $1,200 EAPF-50NH2/1250G PuriFlash Column, 50µ NH2, 1250g $3,050 EAPF-50NH2/2500G PuriFlash Column, 50µ NH2, 2500g $6,500 EA

PF-50NH2HC/6G PuriFlash Column, 50µ NH2 HC, 6g $43 4/PKPF-50NH2HC/20G PuriFlash Column, 50µ NH2 HC, 20g $57 2/PKPF-50NH2HC/35G PuriFlash Column, 50µ NH2 HC, 35g $43 EAPF-50NH2HC/55G PuriFlash Column, 50µ NH2 HC, 55g $68 EAPF-50NH2HC/120G PuriFlash Column, 50µ NH2 HC, 120g $146 EAPF-50NH2HC/175G PuriFlash Column, 50µ NH2 HC, 175g $211 EAPF-50NH2HC/300G PuriFlash Column, 50µ NH2 HC, 300g $362 EAPF-50NH2HC/450G PuriFlash Column, 50µ NH2 HC, 450g $556 EAPF-50NH2HC/1250G PuriFlash Column, 50µ NH2 HC, 1250g $1,461 EAPF-50NH2HC/2500G PuriFlash Column, 50µ NH2 HC, 2500g $2,822 EA

PF-50DEAP/6G PuriFlash Column, 50µ DEAP, 6g $138 6/PKPF-50DEAP/20G PuriFlash Column, 50µ DEAP, 20g $415 6/PKPF-50DEAP/35G PuriFlash Column, 50µ DEAP, 35g $651 6/PKPF-50DEAP/55G PuriFlash Column, 50µ DEAP, 55g $168 EAPF-50DEAP/120G PuriFlash Column, 50µ DEAP, 120g $362 EAPF-50DEAP/175G PuriFlash Column, 50µ DEAP, 175g $527 EAPF-50DEAP/300G PuriFlash Column, 50µ DEAP, 300g $913 EAPF-50DEAP/450G PuriFlash Column, 50µ DEAP, 450g $1,411 EAPF-50DEAP/1250G PuriFlash Column, 50µ DEAP, 1250g $3,684 EAPF-50DEAP/2500G PuriFlash Column, 50µ DEAP, 2500g $7,274 EA

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PuriFlash Normal PhaseNH2 & DEAP Bonded Silica

14 PuriFlash Ion ExchangeSCX & SAX Bonded Silica

PuriFlash Ion ExchangeMM1 Bonded Silica

PuriFlash Reverse PhaseP6 for Natural Products

Part number Description Price UnitPF-50SCX/6G PuriFlash Column, 50µ SCX, 6g $165 4/PKPF-50SCX/20G PuriFlash Column, 50µ SCX, 20g $268 2/PKPF-50SCX/35G PuriFlash Column, 50µ SCX, 35g $212 EAPF-50SCX/55G PuriFlash Column, 50µ SCX, 55g $336 EAPF-50SCX/120G PuriFlash Column, 50µ SCX, 120g $719 EAPF-50SCX/175G PuriFlash Column, 50µ SCX, 175g $1,051 EAPF-50SCX/300G PuriFlash Column, 50µ SCX, 300g $1,832 EAPF-50SCX/450G PuriFlash Column, 50µ SCX, 450g $2,830 EAPF-50SCX/1250G PuriFlash Column, 50µ SCX, 1250g $7,416 EAPF-50SCX/2500G PuriFlash Column, 50µ SCX, 2500g ###### EA

PF-50MM1/6G PuriFlash Column, 50µ MM1 (RP/SCX), 6g $95 4/PKPF-50MM1/20G PuriFlash Column, 50µ MM1 (RP/SCX), 20g $144 2/PKPF-50MM1/35G PuriFlash Column, 50µ MM1 (RP/SCX), 35g $115 EAPF-50MM1/55G PuriFlash Column, 50µ MM1 (RP/SCX), 55g $177 EAPF-50MM1/120G PuriFlash Column, 50µ MM1 (RP/SCX), 120g $377 EAPF-50MM1/175G PuriFlash Column, 50µ MM1 (RP/SCX), 175g $551 EAPF-50MM1/300G PuriFlash Column, 50µ MM1 (RP/SCX), 300g $954 EAPF-50MM1/450G PuriFlash Column, 50µ MM1 (RP/SCX), 450g $1,475 EAPF-50MM1/1250G PuriFlash Column, 50µ MM1 (RP/SCX), 1250g $3,849 EAPF-50MM1/2500G PuriFlash Column, 50µ MM1 (RP/SCX), 2500g $7,610 EA

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Part number Description Price UnitPF-50SAX/6G PuriFlash Column, 50µ SAX, 6g $275 4/PKPF-50SAX/20G PuriFlash Column, 50µ SAX, 20g $375 2/PKPF-50SAX/35G PuriFlash Column, 50µ SAX, 35g $355 EAPF-50SAX/55G PuriFlash Column, 50µ SAX, 55g $540 EAPF-50SAX/120G PuriFlash Column, 50µ SAX, 120g $865 EAPF-50SAX/175G PuriFlash Column, 50µ SAX, 175g $1,075 EAPF-50SAX/300G PuriFlash Column, 50µ SAX, 300g $1,905 EAPF-50SAX/450G PuriFlash Column, 50µ SAX, 450g $3,060 EAPF-50SAX/1250G PuriFlash Column, 50µ SAX, 1250g $8,260 EAPF-50SAX/2500G PuriFlash Column, 50µ SAX, 2500g $16,450 EA

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PF-100P6/2G PuriFlash Column, 100µ P6 Polyamide, 2g $30 4/PKPF-100P6/8G PuriFlash Column, 100µ P6 Polyamide, 8g $40 2/PKPF-100P6/12G PuriFlash Column, 100µ P6 Polyamide, 12g $60 2/PKPF-100P6/20G PuriFlash Column, 100µ P6 Polyamide, 20g $85 2/PKPF-100P6/45G PuriFlash Column, 100µ P6 Polyamide, 45g $88 EAPF-100P6/65G PuriFlash Column, 100µ P6 Polyamide, 65g $124 EAPF-100P6/115G PuriFlash Column, 100µ P6 Polyamide, 115g $209 EAPF-100P6/175G PuriFlash Column, 100µ P6 Polyamide, 175g $321 EAPF-100P6/465G PuriFlash Column, 100µ P6 Polyamide, 465g $819 EAPF-100P6/940G PuriFlash Column, 100µ P6 Polyamide, 940g $1,531 EA

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Quote

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Part number Description Price UnitPF-15C18HP/6G PuriFlash Column, 15µ C18 HP, 6g $390 4/PKPF-15C18HP/20G PuriFlash Column, 15µ C18 HP, 20g $390 2/PKPF-15C18HP/35G PuriFlash Column, 15µ C18 HP, 35g $225 EAPF-15C18HP/55G PuriFlash Column, 15µ C18 HP, 55g $285 EAPF-15C18HP/120G PuriFlash Column, 15µ C18 HP, 120g $545 EAPF-15C18HP/175G PuriFlash Column, 15µ C18 HP, 175g $736 EAPF-15C18HP/300G PuriFlash Column, 15µ C18 HP, 300g $1,281 EAPF-15C18HP/450G PuriFlash Column, 15µ C18 HP, 450g $1,976 EAPF-15C18HP/1250G PuriFlash Column, 15µ C18 HP, 1250g $5,168 EAPF-15C18HP/2500G PuriFlash Column, 15µ C18 HP, 2500g $10,252 EA

PF-30C18HP/6G PuriFlash Column, 30µ C18 HP, 6g $340 4/PKPF-30C18HP/20G PuriFlash Column, 30µ C18 HP, 20g $340 2/PKPF-30C18HP/35G PuriFlash Column, 30µ C18 HP, 35g $195 EAPF-30C18HP/55G PuriFlash Column, 30µ C18 HP, 55g $240 EAPF-30C18HP/120G PuriFlash Column, 30µ C18 HP, 120g $490 EAPF-30C18HP/175G PuriFlash Column, 30µ C18 HP, 175g $540 EAPF-30C18HP/300G PuriFlash Column, 30µ C18 HP, 300g $990 EAPF-30C18HP/450G PuriFlash Column, 30µ C18 HP, 450g $1,490 EAPF-30C18HP/1250G PuriFlash Column, 30µ C18 HP, 1250g $3,445 EAPF-30C18HP/2500G PuriFlash Column, 30µ C18 HP, 2500g $6,995 EA


PF-15C18HQ/6G PuriFlash Column, 15µ C18 HQ, 6g $400 4/PKPF-15C18HQ/20G PuriFlash Column, 15µ C18 HQ, 20g $400 2/PKPF-15C18HQ/35G PuriFlash Column, 15µ C18 HQ, 35g $235 EAPF-15C18HQ/55G PuriFlash Column, 15µ C18 HQ, 55g $295 EAPF-15C18HQ/120G PuriFlash Column, 15µ C18 HQ, 120g $595 EAPF-15C18HQ/175G PuriFlash Column, 15µ C18 HQ, 175g $855 EAPF-15C18HQ/300G PuriFlash Column, 15µ C18 HQ, 300g $1,482 EAPF-15C18HQ/450G PuriFlash Column, 15µ C18 HQ, 450g $2,282 EAPF-15C18HQ/1250G PuriFlash Column, 15µ C18 HQ, 1250g $6,003 EAPF-15C18HQ/2500G PuriFlash Column, 15µ C18 HQ, 2500g $11,925 EA

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PK

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PuriFlash Reverse PhaseC18HP & C18HQ Bonded Silica

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Part number Description Price UnitPF-15C18HP/6G PuriFlash Column, 15µ C18 HP, 6g $390 4/PKPF-15C18HP/20G PuriFlash Column, 15µ C18 HP, 20g $390 2/PKPF-15C18HP/35G PuriFlash Column, 15µ C18 HP, 35g $225 EAPF-15C18HP/55G PuriFlash Column, 15µ C18 HP, 55g $285 EAPF-15C18HP/120G PuriFlash Column, 15µ C18 HP, 120g $545 EAPF-15C18HP/175G PuriFlash Column, 15µ C18 HP, 175g $736 EAPF-15C18HP/300G PuriFlash Column, 15µ C18 HP, 300g $1,281 EAPF-15C18HP/450G PuriFlash Column, 15µ C18 HP, 450g $1,976 EAPF-15C18HP/1250G PuriFlash Column, 15µ C18 HP, 1250g $5,168 EAPF-15C18HP/2500G PuriFlash Column, 15µ C18 HP, 2500g $10,252 EA



PF-15C18HQ/6G PuriFlash Column, 15µ C18 HQ, 6g $400 4/PKPF-15C18HQ/20G PuriFlash Column, 15µ C18 HQ, 20g $400 2/PKPF-15C18HQ/35G PuriFlash Column, 15µ C18 HQ, 35g $235 EAPF-15C18HQ/55G PuriFlash Column, 15µ C18 HQ, 55g $295 EAPF-15C18HQ/120G PuriFlash Column, 15µ C18 HQ, 120g $595 EAPF-15C18HQ/175G PuriFlash Column, 15µ C18 HQ, 175g $855 EAPF-15C18HQ/300G PuriFlash Column, 15µ C18 HQ, 300g $1,482 EAPF-15C18HQ/450G PuriFlash Column, 15µ C18 HQ, 450g $2,282 EAPF-15C18HQ/1250G PuriFlash Column, 15µ C18 HQ, 1250g $6,003 EAPF-15C18HQ/2500G PuriFlash Column, 15µ C18 HQ, 2500g $11,925 EA

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PK

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PuriFlash Reverse PhaseC18HC Bonded Silica

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PKPF-15C18HC/20G PuriFlash Column, 15µ C18 HC, 20g $390 2/PKPF-15C18HC/35G PuriFlash Column, 15µ C18 HC, 35g $225 EAPF-15C18HC/55G PuriFlash Column, 15µ C18 HC, 55g $285 EAPF-15C18HC/120G PuriFlash Column, 15µ C18 HC, 120g $545 EAPF-15C18HC/175G PuriFlash Column, 15µ C18 HC, 175g $736 EAPF-15C18HC/300G PuriFlash Column, 15µ C18 HC, 300g $1,281 EAPF-15C18HC/450G PuriFlash Column, 15µ C18 HC, 450g $1,976 EAPF-15C18HC/1250G PuriFlash Column, 15µ C18 HC, 1250g $5,168 EAPF-15C18HC/2500G PuriFlash Column, 15µ C18 HC, 2500g $10,252 EA

PF-30C18HC/6G PuriFlash Column, 30µ C18 HC, 6g $340 4/PKPF-30C18HC/20G PuriFlash Column, 30µ C18 HC, 20g $340 2/PKPF-30C18HC/35G PuriFlash Column, 30µ C18 HC, 35g $195 EAPF-30C18HC/55G PuriFlash Column, 30µ C18 HC, 55g $240 EAPF-30C18HC/120G PuriFlash Column, 30µ C18 HC, 120g $490 EAPF-30C18HC/175G PuriFlash Column, 30µ C18 HC, 175g $540 EAPF-30C18HC/300G PuriFlash Column, 30µ C18 HC, 300g $990 EAPF-30C18HC/450G PuriFlash Column, 30µ C18 HC, 450g $1,490 EAPF-30C18HC/1250G PuriFlash Column, 30µ C18 HC, 1250g $3,445 EAPF-30C18HC/2500G PuriFlash Column, 30µ C18 HC, 2500g $6,995 EA

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PuriFlash Reverse PhaseHigh Capacity Polymers

Part number Description Price UnitPF-X/2G PuriFlash Column, Atoll X 40µ 2G $103 4/PKPF-X/7G PuriFlash Column, Atoll X 40µ 7G $159 2/PKPF-X/10G PuriFlash Column, Atoll X 40µ 10G $124 EAPF-X/18G PuriFlash Column, Atoll X 40µ 18G $186 EAPF-X/40G PuriFlash Column, Atoll X 40µ 40G $421 EAPF-X/55G PuriFlash Column, Atoll X 40µ 55G $608 EAPF-X/100G PuriFlash Column, Atoll X 40µ 100G $1,061 EAPF-X/150G PuriFlash Column, Atoll X 40µ 150G $1,628 EAPF-X/400G PuriFlash Column, Atoll X 40µ 400G $4,282 EAPF-X/800G PuriFlash Column, Atoll X 40µ 800G $8,492 EA

PF-302H/2G PuriFlash Column, 302H 30µ 2G $143 4/PKPF-302H/7G PuriFlash Column, 302H 30µ 7G $232 2/PKPF-302H/10G PuriFlash Column, 302H 30µ 10G $181 EAPF-302H/18G PuriFlash Column, 302H 30µ 18G $278 EAPF-302H/40G PuriFlash Column, 302H 30µ 40G $621 EAPF-302H/55G PuriFlash Column, 302H 30µ 55G $896 EAPF-302H/100G PuriFlash Column, 302H 30µ 100G $1,577 EAPF-302H/150G PuriFlash Column, 302H 30µ 150G $2,417 EAPF-302H/400G PuriFlash Column, 302H 30µ 400G $6,359 EAPF-302H/800G PuriFlash Column, 302H 30µ 800G ###### EA

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Quote

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Part number Description Price UnitPF-15C18AQ/6G PuriFlash Column, 15µ C18AQ, 6g $390 4/PKPF-15C18AQ/20G PuriFlash Column, 15µ C18AQ, 20g $390 2/PKPF-15C18AQ/35G PuriFlash Column, 15µ C18AQ, 35g $225 EAPF-15C18AQ/55G PuriFlash Column, 15µ C18AQ, 55g $285 EAPF-15C18AQ/120G PuriFlash Column, 15µ C18AQ, 120g $545 EAPF-15C18AQ/175G PuriFlash Column, 15µ C18AQ, 175g $736 EA

PuriFlash Column, 15µ C18AQ, 300g $1,281 EAPF-15C18AQ/450G PuriFlash Column, 15µ C18AQ, 450g $1,976 EAPF-15C18AQ/1250G PuriFlash Column, 15µ C18AQ, 1250g $5,168 EAPF-15C18AQ/2500G PuriFlash Column, 15µ C18AQ, 2500g $10,252 EA

PF-30C18AQ/6G PuriFlash Column, 30µ C18AQ, 6g $340 4/PKPF-30C18AQ/20G PuriFlash Column, 30µ C18AQ, 20g $340 2/PKPF-30C18AQ/35G PuriFlash Column, 30µ C18AQ, 35g $195 EAPF-30C18AQ/55G PuriFlash Column, 30µ C18AQ, 55g $240 EAPF-30C18AQ/120G PuriFlash Column, 30µ C18AQ, 120g $490 EAPF-30C18AQ/175G PuriFlash Column, 30µ C18AQ, 175g $540 EAPF-30C18AQ/300G PuriFlash Column, 30µ C18AQ, 300g $990 EAPF-30C18AQ/450G PuriFlash Column, 30µ C18AQ, 450g $1,490 EAPF-30C18AQ/1250G PuriFlash Column, 30µ C18AQ, 1250g $3,445 EAPF-30C18AQ/2500G PuriFlash Column, 30µ C18AQ, 2500g $6,995 EA

PF-15RPAQ/6G PuriFlash Column, 15µ RPAQ, 6g $390 4/PKPF-15RPAQ/20G PuriFlash Column, 15µ RPAQ, 20g $390 2/PKPF-15RPAQ/35G PuriFlash Column, 15µ RPAQ, 35g $225 EAPF-15RPAQ/55G PuriFlash Column, 15µ RPAQ, 55g $285 EAPF-15RPAQ/120G PuriFlash Column, 15µ RPAQ, 120g $545 EAPF-15RPAQ/175G PuriFlash Column, 15µ RPAQ, 175g $736 EAPF-15RPAQ/300G PuriFlash Column, 15µ RPAQ, 300g $1,281 EAPF-15RPAQ/450G PuriFlash Column, 15µ RPAQ, 450g $1,976 EAPF-15RPAQ/1250G PuriFlash Column, 15µ RPAQ, 1250g $5,168 EAPF-15RPAQ/2500G PuriFlash Column, 15µ RPAQ, 2500g $10,252 EA

PF-30RPAQ/6G PuriFlash Column, 30µ RPAQ, 6g $340 4/PKPF-30RPAQ/20G PuriFlash Column, 30µ RPAQ, 20g $340 2/PKPF-30RPAQ/35G PuriFlash Column, 30µ RPAQ, 35g $195 EAPF-30RPAQ/55G PuriFlash Column, 30µ RPAQ, 55g $240 EAPF-30RPAQ/120G PuriFlash Column, 30µ RPAQ, 120g $490 EAPF-30RPAQ/175G PuriFlash Column, 30µ RPAQ, 175g $540 EAPF-30RPAQ/300G PuriFlash Column, 30µ RPAQ, 300g $990 EAPF-30RPAQ/450G PuriFlash Column, 30µ RPAQ, 450g $1,490 EAPF-30RPAQ/1250G PuriFlash Column, 30µ RPAQ, 1250g $3,445 EAPF-30RPAQ/2500G PuriFlash Column, 30µ RPAQ, 2500g $6,995 EA

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PuriFlash Reverse PhaseHigh Aqueous C18 Bonded Silica

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Part number Description Price UnitPP-15C18T/6G PuriFlash Column, 15µ PP 300Å C18T, 6g $210 2/PKPP-15C18T/20G PuriFlash Column, 15µ PP 300Å C18T, 20g $210 EAPP-15C18T/35G PuriFlash Column, 15µ PP 300Å C18T, 35g $345 EAPP-15C18T/55G PuriFlash Column, 15µ PP 300Å C18T, 55g $545 EAPP-15C18T/120G PuriFlash Column, 15µ PP 300Å C18T, 120g $1,166 EAPP-15C18T/175G PuriFlash Column, 15µ PP 300Å C18T, 175g $1,708 EAPP-15C18T/300G PuriFlash Column, 15µ PP 300Å C18T, 300g $2,989 EAPP-15C18T/450G PuriFlash Column, 15µ PP 300Å C18T, 450g $4,618 EAPP-15C18T/1250G PuriFlash Column, 15µ PP 300Å C18T, 1250g $12,113 EAPP-15C18T/2500G PuriFlash Column, 15µ PP 300Å C18T, 2500g $24,178 EA

PP-30C18T/6G PuriFlash Column, 30µ PP 300Å C18T, 6g $170 2/PKPP-30C18T/20G PuriFlash Column, 30µ PP 300Å C18T, 20g $170 EAPP-30C18T/35G PuriFlash Column, 30µ PP 300Å C18T, 35g $205 EAPP-30C18T/55G PuriFlash Column, 30µ PP 300Å C18T, 55g $245 EAPP-30C18T/120G PuriFlash Column, 30µ PP 300Å C18T, 120g $490 EAPP-30C18T/175G PuriFlash Column, 30µ PP 300Å C18T, 175g $598 EAPP-30C18T/300G PuriFlash Column, 30µ PP 300Å C18T, 300g $1,040 EAPP-30C18T/450G PuriFlash Column, 30µ PP 300Å C18T, 450g $1,602 EAPP-30C18T/1250G PuriFlash Column, 30µ PP 300Å C18T, 1250g $4,191 EAPP-30C18T/2500G PuriFlash Column, 30µ PP 300Å C18T, 2500g $8,290 EA

PP-15C8/6G PuriFlash Column, 15µ PP 300Å C8, 6g $210 2/PKPP-15C8/20G PuriFlash Column, 15µ PP 300Å C8, 20g $210 EAPP-15C8/35G PuriFlash Column, 15µ PP 300Å C8, 35g $330 EAPP-15C8/55G PuriFlash Column, 15µ PP 300Å C8, 55g $521 EAPP-15C8/120G PuriFlash Column, 15µ PP 300Å C8, 120g $1,119 EAPP-15C8/175G PuriFlash Column, 15µ PP 300Å C8, 175g $1,640 EAPP-15C8/300G PuriFlash Column, 15µ PP 300Å C8, 300g $2,868 EAPP-15C8/450G PuriFlash Column, 15µ PP 300Å C8, 450g $4,429 EAPP-15C8/1250G PuriFlash Column, 15µ PP 300Å C8, 1250g $11,621 EAPP-15C8/2500G PuriFlash Column, 15µ PP 300Å C8, 2500g $23,198 EA

PP-30C8/6G PuriFlash Column, 30µ PP 300Å C8, 6g $170 2/PKPP-30C8/20G PuriFlash Column, 30µ PP 300Å C8, 20g $170 EAPP-30C8/35G PuriFlash Column, 30µ PP 300Å C8, 35g $205 EAPP-30C8/55G PuriFlash Column, 30µ PP 300Å C8, 55g $245 EAPP-30C8/120G PuriFlash Column, 30µ PP 300Å C8, 120g $490 EAPP-30C8/175G PuriFlash Column, 30µ PP 300Å C8, 175g $545 EAPP-30C8/300G PuriFlash Column, 30µ PP 300Å C8, 300g $1,005 EAPP-30C8/450G PuriFlash Column, 30µ PP 300Å C8, 450g $1,515 EAPP-30C8/1250G PuriFlash Column, 30µ PP 300Å C8, 1250g $3,687 EAPP-30C8/2500G PuriFlash Column, 30µ PP 300Å C8, 2500g $7,283 EA

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PuriFlash Reverse Phase Biopurification300Å C18 and C8 Bonded Silica

19PuriFlash Reverse Phase Biopurification300Å C4 and 200Å C18 Bonded Silica

Part number Description Price UnitPP-15C4/6G PuriFlash Column, 15µ PP 300Å C4, 6g $210 2/PKPP-15C4/20G PuriFlash Column, 15µ PP 300Å C4, 20g $215 EAPP-15C4/35G PuriFlash Column, 15µ PP 300Å C4, 35g $345 EAPP-15C4/55G PuriFlash Column, 15µ PP 300Å C4, 55g $545 EAPP-15C4/120G PuriFlash Column, 15µ PP 300Å C4, 120g $1,166 EAPP-15C4/175G PuriFlash Column, 15µ PP 300Å C4, 175g $1,708 EAPP-15C4/300G PuriFlash Column, 15µ PP 300Å C4, 300g $2,989 EAPP-15C4/450G PuriFlash Column, 15µ PP 300Å C4, 450g $4,618 EAPP-15C4/1250G PuriFlash Column, 15µ PP 300Å C4, 1250g $12,113 EAPP-15C4/2500G PuriFlash Column, 15µ PP 300Å C4, 2500g $24,178 EA

PP-30C4/6G PuriFlash Column, 30µ PP 300Å C4, 6g $170 2/PKPP-30C4/20G PuriFlash Column, 30µ PP 300Å C4, 20g $170 EAPP-30C4/35G PuriFlash Column, 30µ PP 300Å C4, 35g $205 EAPP-30C4/55G PuriFlash Column, 30µ PP 300Å C4, 55g $245 EAPP-30C4/120G PuriFlash Column, 30µ PP 300Å C4, 120g $490 EAPP-30C4/175G PuriFlash Column, 30µ PP 300Å C4, 175g $598 EAPP-30C4/300G PuriFlash Column, 30µ PP 300Å C4, 300g $1,040 EAPP-30C4/450G PuriFlash Column, 30µ PP 300Å C4, 450g $1,602 EAPP-30C4/1250G PuriFlash Column, 30µ PP 300Å C4, 1250g $4,191 EAPP-30C4/2500G PuriFlash Column, 30µ PP 300Å C4, 2500g $8,290 EA

PT-15C18T/6G PuriFlash Column, 15µ PT 200Å C18T, 6g $210 2/PKPT-15C18T/20G PuriFlash Column, 15µ PT 200Å C18T, 20g $224 EAPT-15C18T/35G PuriFlash Column, 15µ PT 200Å C18T, 35g $359 EAPT-15C18T/55G PuriFlash Column, 15µ PT 200Å C18T, 55g $565 EAPT-15C18T/120G PuriFlash Column, 15µ PT 200Å C18T, 120g $1,213 EAPT-15C18T/175G PuriFlash Column, 15µ PT 200Å C18T, 175g $1,776 EAPT-15C18T/300G PuriFlash Column, 15µ PT 200Å C18T, 300g $3,110 EAPT-15C18T/450G PuriFlash Column, 15µ PT 200Å C18T, 450g $4,800 EAPT-15C18T/1250G PuriFlash Column, 15µ PT 200Å C18T, 1250g $12,596 EAPT-15C18T/2500G PuriFlash Column, 15µ PT 200Å C18T, 2500g $25,150 EA

PT-30C18T/6G PuriFlash Column, 30µ PT 200Å C18T, 6g $170 2/PKPT-30C18T/20G PuriFlash Column, 30µ PT 200Å C18T, 20g $170 EAPT-30C18T/35G PuriFlash Column, 30µ PT 200Å C18T, 35g $205 EAPT-30C18T/55G PuriFlash Column, 30µ PT 200Å C18T, 55g $245 EAPT-30C18T/120G PuriFlash Column, 30µ PT 200Å C18T, 120g $490 EAPT-30C18T/175G PuriFlash Column, 30µ PT 200Å C18T, 175g $598 EAPT-30C18T/300G PuriFlash Column, 30µ PT 200Å C18T, 300g $1,040 EAPT-30C18T/450G PuriFlash Column, 30µ PT 200Å C18T, 450g $1,602 EAPT-30C18T/1250G PuriFlash Column, 30µ PT 200Å C18T, 1250g $4,191 EAPT-30C18T/2500G PuriFlash Column, 30µ PT 200Å C18T, 2500g $8,290 EA

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Part number Description Price UnitPT-15C8/6G PuriFlash Column, 15µ PT 200Å C8, 6g $210 2/PKPT-15C8/20G PuriFlash Column, 15µ PT 200Å C8, 20g $210 EAPT-15C8/35G PuriFlash Column, 15µ PT 200Å C8, 35g $330 EAPT-15C8/55G PuriFlash Column, 15µ PT 200Å C8, 55g $521 EAPT-15C8/120G PuriFlash Column, 15µ PT 200Å C8, 120g $1,119 EAPT-15C8/175G PuriFlash Column, 15µ PT 200Å C8, 175g $1,640 EAPT-15C8/300G PuriFlash Column, 15µ PT 200Å C8, 300g $2,868 EAPT-15C8/450G PuriFlash Column, 15µ PT 200Å C8, 450g $4,429 EAPT-15C8/1250G PuriFlash Column, 15µ PT 200Å C8, 1250g $11,621 EAPT-15C8/2500G PuriFlash Column, 15µ PT 200Å C8, 2500g $23,198 EA

PT-30C8/6G PuriFlash Column, 30µ PT 200Å C8, 6g $170 2/PKPT-30C8/20G PuriFlash Column, 30µ PT 200Å C8, 20g $170 EAPT-30C8/35G PuriFlash Column, 30µ PT 200Å C8, 35g $205 EAPT-30C8/55G PuriFlash Column, 30µ PT 200Å C8, 55g $245 EAPT-30C8/120G PuriFlash Column, 30µ PT 200Å C8, 120g $490 EAPT-30C8/175G PuriFlash Column, 30µ PT 200Å C8, 175g $592 EAPT-30C8/300G PuriFlash Column, 30µ PT 200Å C8, 300g $1,028 EAPT-30C8/450G PuriFlash Column, 30µ PT 200Å C8, 450g $1,587 EAPT-30C8/1250G PuriFlash Column, 30µ PT 200Å C8, 1250g $4,144 EAPT-30C8/2500G PuriFlash Column, 30µ PT 200Å C8, 2500g $8,199 EA

PT-15C4/6G PuriFlash Column, 15µ PT 200Å C4, 6g $210 2/PKPT-15C4/20G PuriFlash Column, 15µ PT 200Å C4, 20g $212 EAPT-15C4/35G PuriFlash Column, 15µ PT 200Å C4, 35g $342 EAPT-15C4/55G PuriFlash Column, 15µ PT 200Å C4, 55g $539 EAPT-15C4/120G PuriFlash Column, 15µ PT 200Å C4, 120g $1,154 EAPT-15C4/175G PuriFlash Column, 15µ PT 200Å C4, 175g $1,690 EAPT-15C4/300G PuriFlash Column, 15µ PT 200Å C4, 300g $2,954 EAPT-15C4/450G PuriFlash Column, 15µ PT 200Å C4, 450g $4,562 EAPT-15C4/1250G PuriFlash Column, 15µ PT 200Å C4, 1250g $11,971 EAPT-15C4/2500G PuriFlash Column, 15µ PT 200Å C4, 2500g $23,896 EA

PT-30C4/6G PuriFlash Column, 30µ PT 200Å C4, 6g $170 2/PKPT-30C4/20G PuriFlash Column, 30µ PT 200Å C4, 20g $170 EAPT-30C4/35G PuriFlash Column, 30µ PT 200Å C4, 35g $205 EAPT-30C4/55G PuriFlash Column, 30µ PT 200Å C4, 55g $245 EAPT-30C4/120G PuriFlash Column, 30µ PT 200Å C4, 120g $490 EAPT-30C4/175G PuriFlash Column, 30µ PT 200Å C4, 175g $598 EAPT-30C4/300G PuriFlash Column, 30µ PT 200Å C4, 300g $1,040 EAPT-30C4/450G PuriFlash Column, 30µ PT 200Å C4, 450g $1,602 EAPT-30C4/1250G PuriFlash Column, 30µ PT 200Å C4, 1250g $4,191 EAPT-30C4/2500G PuriFlash Column, 30µ PT 200Å C4, 2500g $8,290 EA

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PuriFlash Reverse Phase Biopurification200Å C8 & C4 Bonded Silica

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Part number Description Price UnitCF-90A500/7ML PuriFlash Column, 90µ Cellufine DEAE, 7ml $47 2/PKCF-90A500/27ML PuriFlash Column, 90µ Cellufine DEAE, 27ml $71 EACF-90A500/45ML PuriFlash Column, 90µ Cellufine DEAE, 45ml $109 EACF-90A500/68ML PuriFlash Column, 90µ Cellufine DEAE, 68ml $168 EACF-90A500/145ML PuriFlash Column, 90µ Cellufine DEAE, 145ml $362 EACF-90A500/215ML PuriFlash Column, 90µ Cellufine DEAE, 215ml $527 EACF-90A500/375ML PuriFlash Column, 90µ Cellufine DEAE, 375ml $916 EACF-90A500/575ML PuriFlash Column, 90µ Cellufine DEAE, 575ml $1,414 EACF-90A500/1540ML PuriFlash Column, 90µ Cellufine DEAE, 1540ml $3,693 EACF-90A500/3100ML PuriFlash Column, 90µ Cellufine DEAE, 3100ml $7,295 EA

CF-90Q500/7ML PuriFlash Column, 90µ Cellufine QA, 7ml $53 2/PKCF-90Q500/27ML PuriFlash Column, 90µ Cellufine QA, 27ml $82 EACF-90Q500/45ML PuriFlash Column, 90µ Cellufine QA, 45ml $127 EACF-90Q500/68ML PuriFlash Column, 90µ Cellufine QA, 68ml $200 EACF-90Q500/145ML PuriFlash Column, 90µ Cellufine QA, 145ml $427 EACF-90Q500/215ML PuriFlash Column, 90µ Cellufine QA, 215ml $624 EACF-90Q500/375ML PuriFlash Column, 90µ Cellufine QA, 375ml $1,084 EACF-90Q500/575ML PuriFlash Column, 90µ Cellufine QA, 575ml $1,673 EACF-90Q500/1540ML PuriFlash Column, 90µ Cellufine QA, 1540ml $4,373 EACF-90Q500/3100ML PuriFlash Column, 90µ Cellufine QA, 3100ml $8,658 EA

CF-90C500/7ML PuriFlash Column, 90µ Cellufine CM, 7ml $47 2/PKCF-90C500/27ML PuriFlash Column, 90µ Cellufine CM, 27ml $71 EACF-90C500/45ML PuriFlash Column, 90µ Cellufine CM, 45ml $112 EACF-90C500/68ML PuriFlash Column, 90µ Cellufine CM, 68ml $171 EACF-90C500/145ML PuriFlash Column, 90µ Cellufine CM, 145ml $368 EACF-90C500/215ML PuriFlash Column, 90µ Cellufine CM, 215ml $536 EACF-90C500/375ML PuriFlash Column, 90µ Cellufine CM, 375ml $931 EACF-90C500/575ML PuriFlash Column, 90µ Cellufine CM, 575ml $1,437 EACF-90C500/1540ML PuriFlash Column, 90µ Cellufine CM, 1540ml $3,752 EACF-90C500/3100ML PuriFlash Column, 90µ Cellufine CM, 3100ml $7,410 EA

CF-75SO4/7ML PuriFlash Column, 75µ Cellufine SO4, 7ml $124 EACF-75SO4/27ML PuriFlash Column, 75µ Cellufine SO4, 27ml $430 EACF-75SO4/45ML PuriFlash Column, 75µ Cellufine SO4, 45ml $692 EACF-75SO4/68ML PuriFlash Column, 75µ Cellufine SO4, 68ml $1,096 EACF-75SO4/145ML PuriFlash Column, 75µ Cellufine SO4, 145ml $2,350 EACF-75SO4/215ML PuriFlash Column, 75µ Cellufine SO4, 215ml $3,443 EACF-75SO4/375ML PuriFlash Column, 75µ Cellufine SO4, 375ml $6,034 EACF-75SO4/575ML PuriFlash Column, 75µ Cellufine SO4, 575ml $9,318 EACF-75SO4/1540ML PuriFlash Column, 75µ Cellufine SO4, 1540ml ###### EACF-75SO4/3100ML PuriFlash Column, 75µ Cellufine SO4, 3100ml ###### EA

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PuriFlash Cellufine BiopurificationIon Exchange and Affinity

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22 PuriFlash Cellufine BiopurificationEndotoxin Removal and Hydrophobic Interaction

Part number Description Price UnitCF-90EL/7ML PuriFlash Column, 75µ Cellufine Etclean L, 7ml $153 EACF-90EL/27ML PuriFlash Column, 75µ Cellufine Etclean L, 27ml $527 EACF-90EL/45ML PuriFlash Column, 75µ Cellufine Etclean L, 45ml $851 EACF-90EL/68ML PuriFlash Column, 75µ Cellufine Etclean L, 68ml $1,346 EACF-90EL/145ML PuriFlash Column, 75µ Cellufine Etclean L, 145ml $2,892 EACF-90EL/215ML PuriFlash Column, 75µ Cellufine Etclean L, 215ml $4,235 EACF-90EL/375ML PuriFlash Column, 75µ Cellufine Etclean L, 375ml $7,427 EACF-90EL/575ML PuriFlash Column, 75µ Cellufine Etclean L, 575ml ###### EACF-90EL/1540ML PuriFlash Column, 75µ Cellufine Etclean L, 1540ml ###### EACF-90EL/3100ML PuriFlash Column, 75µ Cellufine Etclean L, 3100ml ###### EA

CF-75ES/7ML PuriFlash Column, 75µ Cellufine Etclean S, 7ml $153 EACF-75ES/27ML PuriFlash Column, 75µ Cellufine Etclean S, 27ml $527 EACF-75ES/45ML PuriFlash Column, 75µ Cellufine Etclean S, 45ml $851 EACF-75ES/68ML PuriFlash Column, 75µ Cellufine Etclean S, 68ml $1,346 EACF-75ES/145ML PuriFlash Column, 75µ Cellufine Etclean S, 145ml $2,892 EACF-75ES/215ML PuriFlash Column, 75µ Cellufine Etclean S, 215ml $4,235 EACF-75ES/375ML PuriFlash Column, 75µ Cellufine Etclean S, 375ml $7,427 EACF-75ES/575ML PuriFlash Column, 75µ Cellufine Etclean S, 575ml ###### EACF-75ES/1540ML PuriFlash Column, 75µ Cellufine Etclean S, 1540ml ###### EACF-75ES/3100ML PuriFlash Column, 75µ Cellufine Etclean S, 3100ml ###### EA

CF-90C4/7ML PuriFlash Column, 90µ Cellufine C4, 7ml $65 EACF-90C4/27ML PuriFlash Column, 90µ Cellufine C4, 27ml $215 EACF-90C4/45ML PuriFlash Column, 90µ Cellufine C4, 45ml $342 EACF-90C4/68ML PuriFlash Column, 90µ Cellufine C4, 68ml $539 EACF-90C4/145ML PuriFlash Column, 90µ Cellufine C4, 145ml $1,157 EACF-90C4/215ML PuriFlash Column, 90µ Cellufine C4, 215ml $1,696 EACF-90C4/375ML PuriFlash Column, 90µ Cellufine C4, 375ml $2,966 EACF-90C4/575ML PuriFlash Column, 90µ Cellufine C4, 575ml $4,579 EACF-90C4/1540ML PuriFlash Column, 90µ Cellufine C4, 1540ml ###### EACF-90C4/3100ML PuriFlash Column, 90µ Cellufine C4, 3100ml ###### EA

CF-90PH/7ML PuriFlash Column, 90µ Cellufine Phenyl, 7ml $65 EACF-90PH/27ML PuriFlash Column, 90µ Cellufine Phenyl, 27ml $215 EACF-90PH/45ML PuriFlash Column, 90µ Cellufine Phenyl, 45ml $342 EACF-90PH/68ML PuriFlash Column, 90µ Cellufine Phenyl, 68ml $539 EACF-90PH/145ML PuriFlash Column, 90µ Cellufine Phenyl, 145ml $1,157 EACF-90PH/215ML PuriFlash Column, 90µ Cellufine Phenyl, 215ml $1,696 EACF-90PH/375ML PuriFlash Column, 90µ Cellufine Phenyl, 375ml $2,966 EACF-90PH/575ML PuriFlash Column, 90µ Cellufine Phenyl, 575ml $4,579 EACF-90PH/1540ML PuriFlash Column, 90µ Cellufine Phenyl, 1540ml ###### EACF-90PH/3100ML PuriFlash Column, 90µ Cellufine Phenyl, 3100ml ###### EA

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23PuriFlash Normal Phase Jumbo Packs HP: High Performance Silica

Part number Description Price UnitPF-15SIHP-JP/4G PuriFlash Column, 15µ Si HP, 4g $836 80/PKPF-15SIHP-JP/12G PuriFlash Column, 15µ Si HP, 12g $2,014 80/PKPF-15SIHP-JP/25G PuriFlash Column, 15µ Si HP, 25g $1,843 48/PKPF-15SIHP-JP/40G PuriFlash Column, 15µ Si HP, 40g $2,717 48/PKPF-15SIHP-JP/80G PuriFlash Column, 15µ Si HP, 80g $1,919 16/PKPF-15SIHP-JP/120G PuriFlash Column, 15µ Si HP, 120g $2,755 16/PKPF-15SIHP-JP/200G PuriFlash Column, 15µ Si HP, 200g $2,356 8/PKPF-15SIHP-JP/300G PuriFlash Column, 15µ Si HP, 300g $2,945 8/PKPF-15SIHP-JP/800G PuriFlash Column, 15µ Si HP, 800g $4,655 4/PKPF-15SIHP-JP/1600G PuriFlash Column, 15µ Si HP, 1600g $8,930 4/PK

PF-30SIHP-JP/4G PuriFlash Column, 30µ Si HP, 4g $1,406 160/PKPF-30SIHP-JP/12G PuriFlash Column, 30µ Si HP, 12g $1,463 120/PKPF-30SIHP-JP/25G PuriFlash Column, 30µ Si HP, 25g $1,881 100/PKPF-30SIHP-JP/40G PuriFlash Column, 30µ Si HP, 40g $2,014 80/PKPF-30SIHP-JP/80G PuriFlash Column, 30µ Si HP, 80g $1,577 40/PKPF-30SIHP-JP/120G PuriFlash Column, 30µ Si HP, 120g $1,710 32/PKPF-30SIHP-JP/200G PuriFlash Column, 30µ Si HP, 200g $1,520 16/PKPF-30SIHP-JP/300G PuriFlash Column, 30µ Si HP, 300g $2,413 16/PKPF-30SIHP-JP/800G PuriFlash Column, 30µ Si HP, 800g $1,691 4/PKPF-30SIHP-JP/1600G PuriFlash Column, 30µ Si HP, 1600g $2,983 4/PK

PF-50SIHP-JP/4G PuriFlash Column, 50µ Si HP, 4g $1,007 160/PKPF-50SIHP-JP/12G PuriFlash Column, 50µ Si HP, 12g $1,121 120/PKPF-50SIHP-JP/25G PuriFlash Column, 50µ Si HP, 25g $1,178 100/PKPF-50SIHP-JP/40G PuriFlash Column, 50µ Si HP, 40g $1,178 80/PKPF-50SIHP-JP/80G PuriFlash Column, 50µ Si HP, 80g $851 40/PKPF-50SIHP-JP/120G PuriFlash Column, 50µ Si HP, 120g $1,368 32/PKPF-50SIHP-JP/200G PuriFlash Column, 50µ Si HP, 200g $1,045 16/PKPF-50SIHP-JP/300G PuriFlash Column, 50µ Si HP, 300g $1,577 16/PKPF-50SIHP-JP/800G PuriFlash Column, 50µ Si HP, 800g $950 4/PKPF-50SIHP-JP/1600G PuriFlash Column, 50µ Si HP, 1600g $1,482 4/PK

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HC: High Capacity SilicaPart number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK

PF-25SIHC-JP/4G PuriFlash Column, 25µ Si HC, 4g $1,406 160/PKPF-25SIHC-JP/12G PuriFlash Column, 25µ Si HC, 12g $1,463 120/PKPF-25SIHC-JP/25G PuriFlash Column, 25µ Si HC, 25g $1,881 100/PKPF-25SIHC-JP/40G PuriFlash Column, 25µ Si HC, 40g $2,014 80/PKPF-25SIHC-JP/80G PuriFlash Column, 25µ Si HC, 80g $1,577 40/PKPF-25SIHC-JP/120G PuriFlash Column, 25µ Si HC, 120g $1,710 32/PKPF-25SIHC-JP/200G PuriFlash Column, 25µ Si HC, 200g $1,520 16/PKPF-25SIHC-JP/300G PuriFlash Column, 25µ Si HC, 300g $2,413 16/PKPF-25SIHC-JP/800G PuriFlash Column, 25µ Si HC, 800g $1,691 4/PKPF-25SIHC-JP/1600G PuriFlash Column, 25µ Si HC, 1600g $2,983 4/PK

PF-50SIHC-JP/4G PuriFlash Column, 50µ Si HC, 4g $1,045 160/PKPF-50SIHC-JP/12G PuriFlash Column, 50µ Si HC, 12g $1,159 120/PKPF-50SIHC-JP/25G PuriFlash Column, 50µ Si HC, 25g $1,216 100/PKPF-50SIHC-JP/40G PuriFlash Column, 50µ Si HC, 40g $1,216 80/PKPF-50SIHC-JP/80G PuriFlash Column, 50µ Si HC, 80g $1,273 40/PKPF-50SIHC-JP/120G PuriFlash Column, 50µ Si HC, 120g $1,406 32/PKPF-50SIHC-JP/200G PuriFlash Column, 50µ Si HC, 200g $1,178 16/PKPF-50SIHC-JP/300G PuriFlash Column, 50µ Si HC, 300g $1,615 16/PKPF-50SIHC-JP/800G PuriFlash Column, 50µ Si HC, 800g $988 4/PKPF-50SIHC-JP/1600G PuriFlash Column, 50µ Si HC, 1600g $2,014 4/PK

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Part number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK



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Part number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK



Page 1 of 1

24

Reverse Phase, Linear Scale-up from Analytical to Flash

Solvent:A: Water B: AcetonitrileC: Ammonium formate - 10% in 50/50 Acetonitrile/WaterFlow Rate : Analytical 1.0 ml/min, Flash 20ml/min UV 254nm

puriFlash 15um C18, 35g Part number PF-15C18/35G

Analytical, 250 x 4.6mm 5um C18

0.0

200.0

-200.0

400.0

800.0

1000.0

1200.0

1400.0

maU

600.0

00:00 01:00 02:00 03:00 04:00 05:00 06:00 07:00 08:00

0.99

2

2.78

2

3.50

13.

402

3.71

1

4.25

3

5.97

5

DAD1 A, Sig=215, 4 Ref=360,100 (10-12-06/095-020.D

Are

a: 1

701.

76

Are

a: 6

255.

81

Are

a: 2

667.

88

Are

a: 6

76.2

31

Are

a: 6

129.

713

Are

a: 4

19.5

87

Are

a: 2

076.

46

0.0

50.0

-50.0

100.0

200.0

250.0

300.0

350.0

150.0

05:00 10:00 15:00 20:00 25:00 30:00 35:00 40:00

30

40

20

50

70

80

90

100

60

10

00

%

0.0

200.0

-200.0

400.0

800.0

1000.0

1200.0

1400.0

maU

600.0

00:00 01:00 02:00 03:00 04:00 05:00 06:00 07:00 08:00

0.99

2

2.78

2

3.50

13.

402

3.71

1

4.25

3

5.97

5

DAD1 A, Sig=215, 4 Ref=360,100 (10-12-06/095-020.D

Are

a: 1

701.

76

Are

a: 6

255.

81

Are

a: 2

667.

88

Are

a: 6

76.2

31

Are

a: 6

129.

713

Are

a: 4

19.5

87

Are

a: 2

076.

46

0.0

50.0

-50.0

100.0

200.0

250.0

300.0

350.0

150.0

05:00 10:00 15:00 20:00 25:00 30:00 35:00 40:00

30

40

20

50

70

80

90

100

60

10

00

%

PuriFlash Column Specifications

[email protected] • 800.560.8262www.interchiminc.com

©2011 Interchim Inc. All prices subject to change. 082011

Column TypeSorbentWeight

ID(mm)

Length(mm) Volume

MaxPressure

TypicalFlowRate

FlowRange

Modify the 12g to make a 4g, see ID & Length4g Si,

6g Bonded 12 62 7 ml22 bar / 320 PSI 7ml/min

5-20ml/min

12g Si,20g

Bonded 21 78 27 ml22 bar / 320 PSI

20ml/min

15-50ml/min

25g Si,35g


20ml/min

15-50ml/min

40g Si,55g


30ml/min

20-70ml/min

80g Si,120g

Bonded 31 193 145 ml20 bar / 290 PSI

40ml/min

30-100ml/min

120g Si,175g

Bonded 36 212 215 ml20 bar / 290 PSI

60ml/min

40-150ml/min

200g Si,300g

Bonded 60 133 375 ml20 bar / 290 PSI

160ml/min

80-300ml/min

300g Si, 450g

Bonded 60 205 575 ml20 bar / 290 PSI

160ml/min

80-300ml/min

800g Si, 1250g

Bonded 78 323 1540 ml7 bar /

100 PSI200

ml/min150-300ml/min

1600g Si, 2500g

Bonded 104 365 3100 ml7 bar /

100 PSI400

ml/min200-500ml/min

Coming Soon

2500g Si, 3750g

Bonded 4850 ml7 bar /

100 PSI700

ml/min500-1000

ml/min

0800

0300

0200

0120

0080

0040

0025

0012

0012

1600

2500

110

100

1 000

10 000

100 000

1 000 000

412254080120

200

300

800

1600

Sam

ple

size

(mg)

Column size (g)

puri

Flas

h® C

olum

n Lo

adin

g C

hart

ΔCV

ΔCV

= 1‐1,5 Difficult

ΔCV

= 1,5‐4 Typical

ΔCV

= 4‐10

Easy

CV (A

) = 1/ Rf (A

)CV

(B) =

1/ Rf (B

)ΔC

V = CV (A

) –CV

(B)

Rf (A

)

Rf (B

)

Origin

Solven

t Front

I will

be

addi

ng t

o th

is, p

lace

men

t bac

k co

ver

PURI

FIC

ATIO

N IN

STRU

MEN

TATI

ON

FO

R A

LLIn

terc

him

offe

rs th

e m

ost c

ompr

ehen

sive

rang

e of

inst

rum

ents

from

Fl

ash

to p

repa

rativ

e H

PLC

info

@in

terc

him

inc.

com

w

ww

.inte

rchi

min

c.co

m80

0.56

0.82

62

This

char

t is b

ased

on

usin

g 50

µ H

C sil

ica

colu

mns

und

er n

orm

al p

hase

con

ditio

ns.

Expe

ct tw

o to

thre

e tim

es h

ighe

r loa

ding

with

25µ

and

15µ

silic

a. F

or re

vers

e ph

ase

and

ion

exch

ange

load

ing

refe

r to

page

s 3-6

.in

fo@

inte

rchi

min

c.co

m80

0.56

0.82

62w

ww

.inte

rchi

min

c.co

m

The

nex

T Ti

me

you

rea

ch f

or

a 4

0g f

lash

co

lum

n,

use

a p

uri

fla

sh 2

5g a

nd

geT

500

% h

igh

er l

oa

din

g!

Puri

fic

atio

n in

stru

men

tati

on

fo

r a

llIn

terc

him

offe

rs th

e m

ost c

ompr

ehen

sive

rang

e of

inst

rum

ents

from

flas

h to

pre

para

tive

HPL

C.

• hpl

c pu

mps

for r

elia

bilit

y• d

etec

tion

with

uV,

els

d• s

ame

soft

war

e fo

r fla

sh &

hpl

c

flas

h, e

vo s

erie

s15

0 m

l/min

, 150

PSI

200

ml/m

in, 4

35 P

SI25

0 m

l/min

, 750

PSI

800

ml/m

in, 1

50 P

SI19

x 1

7 x

26”

prep

hpl

c, s

pot i

i50

ml/m

in, 4

350

PSI

250

ml/m

in, 3

300

PSI

500

ml/m

in, 1

600

PSI

24 x

23

x 26

”

Prep

HPL

C, S

pot I

I50

ml/m

in, 4

350

PSI

200

ml/m

in, 3

300

PSI

500

ml/m

in, 1

600

PSI

24" x

23"

x 2

6"

Flas

h, E

vo S

erie

s15

0 m

l/min

, 150

PSI

200

ml/m

in, 4

35 P

SI25

0 m

l/min

, 750

PSI

800

m

l/min

, 150

PSI

19" x

17"

x 2

6"

HPL

C pu

mps

for r

elia

bilit

yD

etec

tion

wit

h U

V, E

LSD

Sam

e so

ftw

are

for F

lash

& H

PLC

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