from images to answers image pro plus 5.1 introduction

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FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

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Page 1: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

FROM IMAGES TO ANSWERS

Image Pro Plus 5.1 Introduction

Page 2: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Plus 5.1More Answers from your Images!

Page 3: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

• Improved Memory Manager– Load Image Sets Larger than

Available RAM– Breaks Through the “Hard

Wall” at 2GB for Windows® Applications* with Image Stacks

– Useful with Today’s Larger Digital Image Stacks

– Works Seamlessly and Silently in the Background

* Cannot open a single image larger than 2GB

Page 4: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

• Macro Player– Load and run the

various macros within IPP

– Demonstrating a range of applications

Page 5: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

• Stitching and Tiling– Align and stitch

images together – Save or load a set

of calculated alignments to be used on Fluorescence images

Page 6: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

• Alignment correction– align individual images, or

images in a sequence, for translation, rotation, or scaling

– Useful for images taken over time, images with some camera movement, aligning channels in multiple images from a beam splitter, or correcting for offset angles through a stereo microscope Z-stack.

Page 7: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

• Improved Automatic Tracking– Correlation Tracking on Active AOI– Full Correlation or Phase

Correlation Options for Tracking under Different Image Conditions

– More than 50 Morphological Measurements available including Intensity Tracking

– Smart Search Radius Detects Object Movement from Image

– New Coherence Filter Adjusts for Neighbor Vectors

– 1-Button Static or Dynamic Intensity Tracking

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Page 8: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Plus 5.1 New Features

Toolbar Extensions• Use Existing Menu Icons

• Create New Icons• Include Self-Contained Macros with

no External Parameters

Page 9: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

IQbase (Now Included with Image Pro Plus)Powering Image Informatics

•Manages Images and Data•Drives Discovery •Facilitates Collaboration•Easy to Use•Works the Way You Do•Grows with Your Organization (Scalable)

Page 10: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

•Gradient painting macro

•Generate Test stripsCreate Surface plot VRML (Virtual Reality Modeling Language )

VRML Viewing plug-in required

(http://www.parallelgraphics.com/products/cortona/download/)

MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

Page 11: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

Page 12: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

Page 13: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

•FRET Calculations

MACROS in IPP (FRET)

Page 14: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Software Series

• Perfect for Basic Imaging or Capture Stations

• More Advanced than Image-Pro Express, Includes Support for Plug-Ins

• The Ultimate Imaging Package, Includes Support for Macros and Plug-Ins

Page 15: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro Advanced Microscopy SuiteThis complete solution for high-level microscopy analysis and acquisition combines Image-Pro Plus 5.1 with powerful tools for multi-dimensional microscope control, image deconvolution, and 3D rendering.

•Image-Pro Multi-Dimensional AcquisitionIdeal for automated microscopy work, this package combines Image-Pro Plus 5.1 with tools for multi-dimensional microscope control and acquisition.

•Image-Pro 3D SuiteIdeal for deconvolution and interactive control and analysis of volume stacks, this package combines Image-Pro Plus 5.1 with deconvolution and rendering tools.

•Image-Pro Microscope ControlThe Image-Pro Microscope Control (MC) package combines Image-Pro Plus Version 5.1 with tools to control and program all major automated microscopes.

Page 16: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro 3D DiscoveryPerfect for confocal microscopy, the Image-Pro 3D Discovery package combines Image-Pro Discovery 5.1 with tools to provide full interaction and analysis of all 3D volumes. It includes major confocal microscope support.

•Image-Pro AnalyzerIdeal for networked users who want advanced analysis tools but do not need image capture functionality at each work station. Image-Pro Analyzer offers all of the powerful analysis features of Image-Pro Plus 5.1, but does not include support for acquisition.10 Unit Network

5 Unit Network

Page 17: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Advanced Microscopy Suite

•Image-Pro 5.1 Advanced Microscopy Suite

•Includes:• Image Pro Plus 5.1•3D Constructor•Sharpstack (SharpStack Plus available as an Option)•AFA•Scope-Pro•IQbase

•AMS Benefits•Lower Customer Prices•Avoids Version Conflicts•One CD Loads All Programs•Upgradeable from Earlier Image Pro Versions

Page 18: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro Multi-Dimensional Acquisition

•Includes:

•Image Pro Plus v. 5.1

•Advanced Fluorescence Acquisition 5.0

•Scope-Pro 5.0

•IQbase

•MDA Benefits•Lower Customer Prices•Avoids Version Conflicts•One CD Loads All Programs•Upgradeable from Earlier Image Pro Versions

Page 19: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro Microscope Control•Includes:

•Image Pro Plus 5.1•Scope-Pro 5.0

•IQbase

•MC Benefits•Lower Customer Prices•Avoids Version Conflicts•One CD Loads All Programs•Upgradeable from Earlier Image Pro Versions

Page 20: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro 3D Suite

•Includes:

•Image Pro Plus v. 5.1

•Advanced Fluorescence Acquisition 5.0

•Scope-Pro 5.0

•IQbase

•MDA Benefits•Lower Customer Prices•Avoids Version Conflicts•One CD Loads All Programs•Upgradeable from Earlier Image Pro Versions

Page 21: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro 3D Discovery•Includes:

•Image Pro Discovery 5.1•3D Constructor 5.0•IQbase

•MC Benefits

•Lower Customer Prices•Upgradeable from Earlier Image Pro Versions•Avoids Version Conflicts•One CD Loads All Programs•Standalone Version Ideal for Confocal Applications

Page 22: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Bundles

•Image-Pro Analyzer•Includes:

•Image Pro Plus v. 5.1 Network Analysis Only (No Capture) •IQbase

Analyzer Benefits•Lower Customer Prices (Network 5 or 10 Seats)

Page 23: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Multi-Dimensional Acquisition

Page 24: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Multi-Dimensional Acquisition

Page 25: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro Microscope Control

• Software control of stages, filter wheels, shutters, z-motors, microscopes

• Ludl, Marzhauzer, Prior, ASI and Objective Imaging

• Leica, Olympus, Zeiss, Nikon

• Sutter, Physik

Page 26: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite

• 3D reconstruction, visualization and volumetric and spatial measurements

• Major Features– Free Rotation of Image

Stacks– Extensive Animation &

Display Tools– Orthogonal & Oblique

Sectioning – Numerous Spatial &

Volumetric Measurements– Time Resolved Tracking of

Objects

Page 27: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite Spatial Measurements

Page 28: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite Automatic Volumetric Measurements

Page 29: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite Automatic Volumetric Measurements Over Time4D Tracking of objects!!

Page 30: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite Neuron Analyzing (Neuron Spines) !!

Page 31: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image Pro 3D Suite

3D Suite Includes:

No Neighbor,

Nearest Neighbor Haze Removal and

Inverse Filter Haze Restoration Algorithms

These Algorithms are not Appropriate for Confocal Images

An Option is Available which Includes 2D Blind and 3D Blind Deconvolution Algorithms

Page 32: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Image-Pro Family Modules

•Automate Motorized Microscopes or Devices (available separately until 12/2004)

• Color-Pro Color Matching System for Camera, monitor and printer

•SharpStack Plus includes: 3D Blind, 2D Blind deconvolution algorithms as well as No Neighbor, Nearest Neighbor and Inverse Filter algorithms

Page 33: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Color-Pro Module

• Consistent Color• Better Quantitative

Results• ICC calibration tools

for camera, screen, printer and projector

Page 34: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Evolution VF - cooled & un cooled- mono & color

• Features– Designed for high resolution

brightfield scientific and industrial applications.

– A progressive scan interline CCD sensor gives a resolution of 1.3 million pixels in a 12-bit digital output. (Sony 205 Sensor)

– High-speed low noise electronics provide linear digital data

– Live Frame rates of up to 110 fps with reduced region of interest and binning.

– The IEEE 1394 FireWire™ digital interface allows ease of use

Page 35: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Micropublisher 3.3 Integrated Color Camera Kit

•High resolution 3.3 megapixel color camera•Features

•Fully integrated with your choice of Image-Pro software•FireWire (IEEE 1394) C-mount camera•Max 25 fps at 640x480 (30-bit) Real Time Video (RTV)•Option for cooling •No interpolation or Pixel shifting•Laptop kit

Page 36: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Evolution MP™ Integrated Color Camera Kit

• High resolution 5.1 megapixel Color Camera (Sony 282 Sensor)

• Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394)

C-mount camera– Max 25 fps at 640x480 (30-bit)

Real Time Video (RTV)– Option for cooling – No interpolation or Pixel shifting– Laptop kit

Page 37: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Evolution QEi™ Integrated Camera Kit

High Quantum Efficiency

Camera

Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394) C-mount camera– Fast FireWire Live 15 fps at 1360x1036(12-

bit)

Ideal for demanding

Lowlight and fluorescence

imaging

Page 38: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Evolution Camera Series

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With IR Filter (Std)Without IR Filter (Removed)

Page 39: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Evolution™ Camera Series

Media Cybernetics

Evolution MP 5.1

Media Cybernetics

Evolution VFMedia Cybernetics

Evolution QEi

Resolution 2560 X 1920 1392 X 1040 1360 X 1036

CCD Type ICX282 (Color/Mono) ICX205 (Color/Mono) ICX205 (Color/Mono)

Sensor Grade Zero Defect Zero Defect Zero Defect

Pixel Size 3.4 μm × 3.4 μm 4.65 μm × 4.65 μm 6.45 μm × 6.45 μm

Full Well 8,600e- 10,000e- 18,000e-, 22,000@2x2

Read Noise 9.8e- 12e- 8e-

Dark Current 2.3e-/p/s (cooled) 1e-/p/s (cooled) 0.15e-/p/s (cooled)

Optical Interface 2/3” C Mount ½” C Mount 2/3” C Mount

Depth 36-bit 12-bit(mono) 36-bit 12-bit(mono)

Cooling 10° C below ambient 25° C below ambient 25° C below ambient

Max live size 2560 X 1920 1392 X 1040 1360 X 1036

Live fps 3 fps (full) 15fps (full) @ 12-bit 15fps (full) @ 12-bit

30fps@640X480 (RTV)Binning (Max) 4X4 8X8 8X8

Gain Control 0 – 10X 0 – 15X 0 – 35X ROI (User defined) Yes Yes Yes

RGB Filter Option Yes Yes

Warranty 2 yrs 2 yrs 2 yrs

Page 40: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Bright field, Phase- Contrast, & Darkfield MicroscopyFluoresecence MicroscopyLive- Cell ImagingPathology, Histology, & CytologyGreen Fluorescent Protein (GFP) ApplicationsFISHCa++ Ratio, TIRFM, AnalysisMotility & Motion AnalysisDNA AnalysisMetallurgical MicroscopySemiconductor InspectionManufacturing Quality ControlFailure AnalysisForensic AnalysisSpinning Disk Confocal system

Applications Evolution QEi™

Page 41: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Applications Evolution VF™

Brightfield, Phase- Contrast, & Darkfield MicroscopyFluoresecence MicroscopyLive- Cell ImagingPathology, Histology, & CytologyMotility & Motion AnalysisDNA AnalysisMetallurgical MicroscopySemiconductor InspectionFailure AnalysisForensic Analysis

Page 42: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Applications Evolution MP5.1™

Brightfield, Phase- Contrast, & Darkfield MicroscopyFluoresecence MicroscopyPathology, Histology, & CytologyHaematologyDocument ImagingStill Image Animation

Page 43: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

300 400 500 600 700 800 900 1000 1100

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DAPIGFP TRITC

CY5

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RT SE6 with IR Filter RT SE6 without IR Filter

Orca ER150

CoolSNAP HQ Mono

Sensicam QE LOW LIGHT

Sensicam QE Standard Mode

Page 44: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Pro-Series MV-Pro & MV-Lite Capture Kits

•Board, Cable & Driver Included

• Works with Image Pro Express, Discovery or Plus

• Works with Most Video Cameras

• Specialized Cables also Available

•New version available soon

Page 45: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

Turboscan

Fast Mosaic Image Acquisition For Image-Pro® Plus

•Scanning and acquisition at camera frame ratesSupport for various analog and digital cameras, including Media Cybernetics' Evolution VF and QImaging's QICAM cameras High-quality image tiling •Frame-rate shading correction •Fast and accurate relocation •Continuous focus tracking using predictive focus •Compatible with most 3rd party motorized stage and focus drives •Intelligent memory management supports mosaic sizes limited only by available disk space •Plug-in capture driver for use within Image-Pro® Plus 5.1

Page 46: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

X, Y or Z Stage Kit

•X,Y-axis Stage and/or Z-axis motor•Controller box• Use With Image Pro 5.1 MC or MDA, AMS• All necessary cables

Page 47: FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

www.mediacy.comSilver Spring, MD (Headquarters)

Offices in:

Boston, MA, Pittsburgh, PA & San Diego, CA

Leiden, The Netherlands

Goettingen, Germany

Wokingham, United Kingdom

Singapore, Republic of Singapore

Tokyo, Japan

Shanghai, China