genetics of bacteria and...
TRANSCRIPT
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Chapter 15Genetics of Bacteria and
Bacteriophages
Copyright © 2010 Pearson Education Inc.
CORSO GENETICA LAUREA TRIENNALE “ROMA TRE”
Prof. A. Antoccia
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ANALISI GENETICA NEI BATTERI:
MAPPATURA MEDIANTE
A) CONIUGAZIONE
B) TRASFORMAZIONE
C) TRASDUZIONE
(GENERALIZZATA,SPECIALIZZATA)
RIPRODUZIONE ASESSUATE E
PROCESSI PARASESSUALI ATTRAVERSO LA
RICOMBINAZIONE-
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UNIDIREZIONALITA’ DEL TRASFERIMENTO
GENICO
DIPLOIDI PARZIALI
NON IN TUTTI I
TIPI DI BATTERI
CON ECCEZIONE
TRASDUZIONE
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TERRENI LIQUIDI E SOLIDI -
TERRENI SELETTIVI
MUTANTI PROTOTROFI E
AUXOTROFI (trp+ o trp-)
CAPACITA’ DI
UTILIZZARE ZUCCHERI
(lac+ o lac-)
RESISTENZA A FARMACI
ED ANTIBIOTOICI (ampR O ampS)
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CONIUGAZIONE IN E. COLI- 1946 LEDEBERG & TATUM-
RARI PROTOTROFI 1 IN 106 CELLULE
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TUBO DI DAVIS
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1953-W. HAYES, UNIDIREZIONALITA’ DELLO SCAMBIO
GENETICO IN E. COLI
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FATTORE F
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CELLULA
DONATRICE
CELLULA
RICEVENTE
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CEPPI DI E. COLI AD ALTA FREQUENZA DI
RICOMBINAZIONE:
High Frequency of Recombination
HFR H - (Hayes) - Sito di integrazione F, in
Prossimità di thr e leu
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FATTORI F’
E SEXDUZIONE
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MAPPATURA
PER
CONIUGAZIONE
1957- F. Jacob e E.
Wollman
Hfr H
thr+ leu+ azas tons
lac+ gal + strs
X F-
Thr- leu- azar tonr lac-
gal- strr
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MEZZO SELETTIVO CON
STR E MANCANTE THR E
LEU, POI TRASFERITE
IN ALTRI MEZZI SELETTIVI
DONATRICI ERANO STR-
E THR+ LEU+
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MAPPA per trasferire tutto cromosoma da ceppo
HFR aricevente F- circa 100 minuti
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MAPPATURA DEI GENI NEI BATTERI PER
TRASFORMAZIONE
(SOLO NEI RICEVENTI COMPETENTI CHE
PRESENTANO RECETTORI SPECIFICI)
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FRAMMENTI PARI ALLO 0.2-0.5% DEL CROMOSOMA
BATTERICO
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IL DNA A SINGOLO FILAMENTO VIENE
PROTETTO DA PROTEINE DI RIVESTIMENTO
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