List of Abstracts by Session 8th International Conference on HHV-6 & 7

Host-Cell Interactions

Entry of cytomegalovirus into cells: Comparison with HHV-6Jeffrey Cohen1

1Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

1-1

Human herpesvirus-6A/B entry into host cellsYasuko Mori1,2, Huamin Tang1, Yoshikazu Furusawa1, Akiko Kawabata1, Megumi Ota1,2, Koichi Yamanishi31Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan2Laboratory of Virology and Vaccinology, National Institute of Biomedical Innovation, Ibaraki, Japan3National Institute of Biomedical Innovation, Ibaraki, Japan

1-2

Identification of the cellular factor interacted with human herpesvirus6 glycoprotein M (gM) /gN complexAkiko Kawabata1, Megumi Ota1, Mayuko Hayashi1, Koichi Yamanishi3, Yasuko Mori1,2

1Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan2Laboratory of Virology and Vaccinology, National Institute of Biomedical Innovation, Ibaraki, Japan3National Institute of Biomedical Innovation, Ibaraki, Japan

1-3

Early events in HHV-6B infection: Binding to CD46 and entry of virusAida Solhøj Hansen1, Pernille Bogetofte Thomasen1, Per Höllsberg1

1Department of Biomedicine, Aarhus University, Aarhus, Denmark

1-4

Phosphorylation of lamin A/C during nuclear capsid egress of beta-herpesvirusesJens Milbradt1, Mirko Kummer2, Sabrina Wagner1, Benedikt Kaufer3, Alexander Steinkasserer2, ManfredMarschall11Institute for Clinical and Molecular Virology, University of Erlangen-Nuremberg, Erlangen, Germany2Department of Dermatology, University of Erlangen-Nuremberg, Erlangen, Germany3Department of Microbiology and Immunology, Free University Berlin, Berlin, Germany

1-5

A simplified purification and concentration method on Nycodenz® forHHV-6 productionAngélique N Godet1, Emiliana Abrao1, Pascale Bonnafous1, Nathalie Désiré1, Agathe Milovanovitch1, HenriAgut1, Agnès Gautheret-Dejean1

1Laboratoire de Virologie, ER1DETIV, UPMC, Hôpitaux Universitaires Pitié Salpêtrière - Charles Foix, Paris,France

1-6

Correlation of human herpesvirus-6 load with NK, CD8+T, !" T cellsand MICA/B in Tunisian patients with acute leukemiaFaten Nefzi1, Lambert Claude2, Agnès Gautheret-Dejean3, Nadia Ben Fredj1, Henri Agut3, Selma Feki4,Mahjoub Aouni11Laboratory of Transmissible Diseases and Biological Active Substances, LR99ES27, Faculty of Pharmacy,University of Monastir, Tunisia2Immunology Laboratory Universitary Hospital CHU Saint-Etienne, Saint-Etienne, France3Laboratory of Virology, Universitary Hospital Pitié-Salpêtrière Charles-Foix, AP-HP, Paris, France4Department of Clinical Biology, Faculty of Pharmacy, Monastir, Tunisia

1-7

Genes & Proteins

Enzymatic and biological properties of HHV-6 u94/rep proteinFrédéric Trempe1, Guillaume Morissette1, Annie Gravel1, Louis Flamand1

1Division of Infectious Diseases and Immunity, CHU Research Center and Faculty of Medicine, LavalUniversity, Quebec, Canada

2-1

The HHV-6 latency-associated U94 disrupts fundamental humanoligodendroglial precursor cell biological and metabolic processesDavid Mock1, Christophe Proschel1, Joseph Hollenbaugh1, Andrew Goodman1, Daniel Tanner1, ChristopherFolts1,Margot Mayer-Proschel11University of Rochester, Department of Biomedical Genetics, Rochester, NY, USA

2-2

Protein composition of HHV-6B strain Z29: LC-MS protein identificationand label-free quantitative analysisAniuska Becerra-Artiles1, Karin Green2, J Mauricio Calvo-Calle1, Scott Shaffer2, Lawrence Stern3

1Department of Pathology, University of Massachusetts Medical School, Worcester, MA, USA2Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School,Worcester, MA, USA3Department of Pathology and Department of Biochemistry and Molecular Pharmacology, University ofMassachusetts Medical School, Worcester, MA, USA

2-3

U69 v-Cdks phosphorylate retinoblastoma family proteins Rb, p107and p130Robert Kalejta1, Satoko Iwahori11Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA

2-4

U20 inhibits death receptor and pattern-recognition receptor signalingin vitro, but increases inflammation in vivoEmil Kofod-Olsen1, Rasmus O Bak1, Søren B Jensen1, Frederik Dagnæs-Hansen1, Christian Holm1, Kent WDeleuran1, Per Höllsberg1

1Department of Biomedicine, Aarhus University, Aarhus, Denmark

2-5

HHV-6 alters E2F1/RB pathways and kidnaps the E2F1 transcriptionfactor to transcribe viral genesEyal Sharon1, Ludmila Volchek2, Niza Frenkel31Department of Cell Research and Immunology, S. Daniel Abraham Institute for Molecular Virology, Tel AvivUniversity, Tel Aviv, Israel

2-6

Characterization of Human Herpesvirus 6a encoded miRNAsMasatoshi Nukui1, Yasuko Mori2, Eain A Murphy1

1Molecular Genetics & Virology Department, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio,USA2Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan

2-7

HHV-6B DR6 protein suppresses cell proliferation in a P53-DependentmannerMariane Schleimann1, Søren Hoberg1, Bettina Bundgaard1, Per Höllsberg1

1Department of Biomedicine, Aarhus University, Aarhus, Denmark

2-8

Inhibition of death signals by the extracellular domain of a viralmembrane proteinDea Kejlberg Jensen1, Emil Kofod-Olsen1, Mariane Høgsbjerg Schleimann1, Per Höllsberg1

1Department of Biomedicine, Aarhus University, Aarhus, Denmark

2-9

HHV-6A infection induces the expression of HERV-W envelope in TlymphocytesJosephine Reynaud1, Gilda Raguenez2, Hei-Lanne Reynaud-Dougier2, Patrice Marche2, Branka Horvat1

1International Center for Infectiology Research, INSERM U1111, Lyon, France2INSERM U823, Université J Fourier, IAPC, Grenoble, France

2-10

Complete genome sequence of the original human herpesvirus 6A GSisolateAnnie Gravel1, Frédéric Raymond1, Jacques Corbeil2, Dharam Ablashi3, Louis Flamand2

1Division of Infectious Diseases and Immunity, CHU Research Center, Laval University, Quebec, Canada2Faculty of Medicine, Laval University, Quebec, Canada3HHV-6 Foundation, Santa Barbara, CA, USA

2-11

Innate & Acquired Immunity

Immunomodulation by HHV-6A and HHV-6BPaolo Lusso1

1Laboratory of Immunoregulation, NIAID, NIH

3-1

Identification and characterisation of CD8+ T cell resonses to HHV-6Bantigens U11 and U90Mustafa Halawi1, Naeem Khan2, Neil Blake1

1Institute of Infection and Global Health, University of Liverpool, Liverpool, UK2Clinical Immunology, School of Immunity and Infection, University of Birmingham, Birmingham, UK

3-2

Comparison of U54 specific T-Cell immunity in individuals with andwithout chromosomally integrated HHV-6 (ciHHV-6)Volker Strenger1,2, Simone Kayser1, Dirk Lassner3, Markus Seidel2, Wolfgang Schwinger2, Stephan W. Aberle4,Gerhard Jahn5, Stefan Jürgens5, Christian Urban2, Tobias Feuchtinger1

1University Children's Hospital, Eberhard-Karls University, Tuebingen, Germany2Department of Paediatrics and Adolescent Medicine, Medical University of Graz, Graz, Austria3Institute Cardiac Diagnostics und Therapy (IKDT), Berlin, Germany4Clinical Institute of Virology, Medical University of Vienna, Vienna, Austria5Institute of Medical Virology, Eberhard-Karls University, Tuebingen, Tuebingen, Germany

3-3

Cross-sectional analysis of CD8+ T cell responses to humanherpesvirus 6BLarissa Martin1, Andreas Moosmann1

1Clinical Cooperation Group Immunooncology, University of Munich Clinical Center and Helmholtz Center,Munich, Germany

3-4

Identification of HLA class I restricted CD8+ T cell epitopesrecognizing HHV-6 IE1 proteinMathieu Iampietro1, Guillaume Morissette1, Annie Gravel1, Louis Flamand1

1Department of Infectious and Immune diseases, Laval University, Sainte-Foy, Canada

3-5

Fine analysis of the human antibody response to the U11 protein fromHHV-6J Mauricio Calvo-Calle1, Lawrence Stern2

1Department of Pathology, School of Medicine, University of Massachusetts, Amherst, MA, USA2Department of Pathology, and Department of Biochemistry and Molecular Pharmacology, School ofMedicine, University of Massachusetts, Worchester, MA, USA

3-6

HHV-6A infection modulates host cell antiviral defense by targeting ERand mitochondriaChristine Siegl1, Bhupesh Prusty1, Yasuko Mori2, Thomas Rudel11Department of Microbiology, University of Wuerzburg, Wuerzburg, Germany2Department of Microbiology and Infectious Diseases, Kobe University, Kobe, Japan

3-7

Studies on HHV-7 induced cytokinesBalázs Stercz1, José Menezes2, Károly Nagy1, Joseph Ongrádi11Laboratory of Immunovirology, Institute of Medical Microbiology, Semmelweis University, Budapest,Hungary2Laboratory of Immunovirology, Department of Microbiology and Immunology, University of Montreal,Montreal, Canada

3-8

Immunosuppressive properties of HHV-6 U54 tegument proteinMathieu Iampietro1, Guillaume Morissette1, Annie Gravel1, Louis Flamand1

1Department of Infectious and Immune Diseases, Laval University, Sainte-Foy, Canada

3-9

Human herpesvirus-6 suppresses T cell proliferation through theinduction of infected cell cycle arrest in G2/M phaseLingyun Li1, Bin Gu2, Feng Zhou1, Jing Chi1, Fang Wang3, Guangyong Peng4, Kun Yao1

1Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China2Department of Neurosurgery, The 1st Affiliated Hospital of Nanjing Medical University, Nanjing, China3Department of Laboratory Medicine, The 1st Affiliated Hospital of Nanjing Medical University, Nanjing,China4Division of Infectious Diseases, Allergy & Immunology and Department of Internal Medicine, Saint LouisUniversity, Saint Louis, MO, USA

3-10

Phenotypic and functional alterations of human peripheralplasmacytoid dendritic cells induced by human herpesvirus 6 infectionin vitroMarina Illiaquer1, Patrice Chevallier2, Nelly Robillard3, Cecile Peltier1, Celine Bressollette-Bodin1, ThierryGuillaume2,Mohamad Mohty2, Berthe-Marie Imbert-Marcille1

1Service de virologie, CHU Nantes, Nantes, France; UPRES-EA4271, PRES L'UNAM, Universite de Nantes,France2Service d'Hematologie Clinique, CHU Nantes, Universite de Nantes, France3Service d'Hematologie Biologie, CHU Nantes, Nantes, France

3-11

Chromosomally Integrated HHV-6

Investigation of the effect of CI-HHV-6 on telomeres and the excisionof the viral genomeYan Haung1, Enjie Zhang1, Alberto Hidalgo-Bravo1, Aaron Mendez-Bermudez1, Victoria Cotton1, Martin Dyer2,Duncan Clark3, Nicola Royle1

1Department of Genetics, University of Leicester, Leicester, UK2Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK3Department of Virology, Royal London Hospital, Barts Health NHS Trust, London, UK

4-1

Persistent human herpesvirus-6 infection in patients with an inheritedform of the virusShara Pantry1, Maria Medveczky1, Jesse Arbuckle1, Janos Luka2, Jose Montoya3, Dharam Ablashi4, JoshuaPritchett4, Peter Medveczky1

1Department of Molecular Medicine, University of South Florida, Tampa, FL, USA2Bioworld Consulting Laboratories, Mt. Airy, MD, USA3Department of Infectious Disease, Stanford University, Palo Alto, CA, USA4HHV-6 Foundation, Santa Barbara, CA, USA

4-2

Mapping the telomere integrated genome of human herpesvirus 6Aand 6BJesse Arbuckle1, Shara Pantry1, Maria Medveczky1, Joshua Pritchett2, Dharam Ablashi2, Peter Medveczky1

1University of South Florida College of Medicine, Tampa, FL, USA2HHV-6 Foundation, Santa Barbara, CA, USA

4-3

Chlamydia infection induces CIHHV6 replication without any infectiousvirus productionBhupesh Prusty1, Christine Siegl1, Georg Krohne2, Thomas Rudel11Biocenter, Chair of Microbiology, University of Wuerzburg, Wuerzburg, Germany2Biocenter, Electron Microscopy Core Facility, University of Wuerzburg, Wuerzburg, Germany

4-4

Dual roles of telomeric repeats in chromosomally integrated humanherpesvirus-6Tamae Ohye1, Hidehito Inagaki1, Masaru Ihira2, Yuki Higashimoto3, Tetsushi Yoshikawa4, Hiroki Kurahashi11Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University,Toyoake, Japan2Faculty of Clinical Engineering, Fujita Health University of Health Sciences, Toyoake, Japan3Department of Laboratory Medicine, Fujita Health University Hospital, Toyoake, Japan4Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan

4-5

Long term treatment of chromosomally integrated human herpesvirus6 in patients with acquired cardiomyopathies and heart failuresymptomsDirk Lassner1, Christine Siegismund1, Felicitas Escher2, Ulrich MUM Gross1, Gerhard RF Krüger3, CarstenTschoepe2

Heinz-Peter Schultheiss2, Uwe Kuehl21IKDT Institute Cardiac Diagnostics und Therapy, Berlin, Germany2Department of Cardiology and Pneumology, Charité Campus Benjamin Franklin, Berlin, Germany3Department of Pathology, University Cologne, Cologne, Germany

4-6

Active HHV-6 infection in ciHHV-6 : Case reportMary Caserta1, Jennifer Carnahan1, Brooke Hamilton2, Kenneth Schnabel1, Lynne Shelley1, Steven Gill21University of Rochester School of Medicine and Dentistry, Department of Pediatrics, Division of InfectiousDiseases, Rochester, NY, USA2University of Rochester School of Medicine and Dentistry, Department of Microbiology and Immunology,Rochester, NY, USA

4-7

Chromosomally integrated human herpesvirus-6 was activated in apatient with X-linked severe combined immunodeficiencyAkifumi Endo1, Kohsuke Imai1, Harutaka Katano2, Naoki Inoue2, Hiroki Kurahashi3, Tetsushi Yoshikawa3,Tomohiro Morio1, Shuki Mizutani11Tokyo Medical and Dental University, Tokyo, Japan2National Institute of Infectious Diseases, Bethesda, MD, USA3Fujita Health University, Toyoake, Japan

4-8

Brief Overview of ciHHV6: Key Points for DiscussionPhillip E. Pellett1,1Department of Immunology and Microbiology, Wayne State University, School of Medicine

4-9

Weak and random reactivation of HHV-6 in chromosomally integratedviral cell lines in the presence of sodium valproate or phenytoinMustafa Al Jawhari1, Hazar Al Mouhammad1, Vincent Descamps2, Sylvie Ranger-Rogez1

1Department of Microbiology, Pharmacy Faculty, Limoges, France2Dermatology Department, Bichat-Claude Bernard Hospital, Paris, France

4-10

Role of the herpesvirus telomeric repeats and the U94 protein inhuman herpesvirus 6 integrationNina Wallaschek1, Mandy Glass2, Anne Paredes3, Dong Yu3, Yasuko Mori4, Benedikt B Kaufer1

1Institute of Virology, Free University Berlin, Berlin, Germany2MRC-Center for Virus Research, University of Glasgow, Glasgow, UK3Department of Molecular Microbiology, Washington University, St. Louis, Missouri, USA4Division of Clinical Virology, Kobe University, Kobe, Japan

4-11

Detection of HHV-6 specific mRNA in individuals with chromosomallyintegrated HHV-6Elisabetta Caselli1, Volker Strenger2, Dario DiLuca1, Wolfgang Schwinger2, Christian Urban2

1Department of Medical Sciences, Microbiology Unit, University of Ferrara, Ferrara, Italy2Department of Paediatrics and Adolescent Medicine, Medical University of Graz, Graz, Austria

4-12

Epidemiology

SNP genotyping of chromosomally integrated human herpesvirus 6 inpatients with cardiomyopathies/myocarditis, childhood malignanciesor neurological syndromesUrsula Gompels1, Maria-Alexandra Spyrou1, Joshua Tweedy1, B Bhandari1, Petr Hubacek2, Uwe Kuhl3, DirkLassner4, and Michael W Gaunt1

1Department Pathogen Molecular Biology, London School of Hygiene & Tropical Medicine, University ofLondon, London, UK2Department of Medical Microbiology and Department of Paediatric Haematology and Oncology, 2nd MedicalFaculty of Charles University and Motol University Hospital, Prague, Czech Republic3Department of Cardiology and Pneumology, Charité Campus Benjamin Franklin, Berlin, Germany4Institute Cardiac Diagnostics und Therapy (IKDT), Berlin, Germany

5-1

Effects of human herpesvirus-6A infection on infant cognitivedevelopmentKunda Musonda1, Daniela Manno2, Marta Sanz-Ramos1, Matthew Bates1, Mwaka Monze3, Suzanne Filteau2,Ursula Gompels1

1Department of Pathogen Molecular Biology, London School Hygiene & Tropical Medicine, University ofLondon, UK2Departments Nutriont & Public Health and Infectious Disease Epidemiology, London School Hygiene &Tropical Medicine, University of London, UK3Virology Unit, University Teaching Hospital, Lusaka, Zambia

5-2

Prevalence of HHV-6A, HHV-6B and HHV-7 in sera specimens from HIVpositive and negative children under 2 years of age admitted to a largereferral hospital in sub-Saharan AfricaJohn Tembo1*, Lophina Chilukutu1 Sylvester Sinyangwe2, Chishala Chabala2, Kunda Musonda3, UrsulaGompels3, Omar Siddiqi 4,5, P Kaonga4, Shabir Lakhi4, Peter Mwaba6, Alimuddin Zumla7, Matthew Bates1,7*1Research & Training Programme and HerpeZ, University of Zambia and University College London MedicalSchool, Lusaka, Zambia2Department of Paediatrics and Child Health, University Teaching Hospital, Lusaka, Zambia3Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, UK4Department for Internal Medicine, University Teaching Hospital, Lusaka, Zambia5Department of Neurology and the Division of Viral Pathogenesis, Division of Neurovirology, Beth IsraelDeaconess Medical Center, Harvard Medical SchooL, Boston, MA, USA6Zambian Ministry of Health, Lusaka, Zambia7Division of Infection and Immunity, University College London, UK

*Authors contributed equally

5-3

Chromosomally integrated HHV-6 in patients with hematologicalpathologiesMustafa Al Jawhari1, Claire Laforest1, Radhia M Kacher2, Catherine Yardin3, Estelle Troadec4, NathalieGachard4, Arnaud Jaccard5, Sylvie Ranger-Rogez1

1Department of Microbiology, Pharmacy Faculty, Limoges, France2Alternative Energies and Atomic Energy Commission, Fontenay-aux-Roses, France3Cytogenetics Department, University Hospital, Limoges, France4Biological Hematology Department, University Hospital, Limoges, France5Clinical Hematology Department, University Hospital, Limoges, France

5-4

Increased prevalence of chromosomally integrated HHV-6 atchromosome 11p telomeric region in pediatric adrenal tumors

Emilia M Pinto1, 2, Troy Lund4, Gerard P Zambetti2, Raul C Ribeiro1, 3

1International Outreach Program, St Jude Children's Research Hospital, Memphis, TN, USA2Department of Biochemistry, St Jude Children's Research Hospital, Memphis, TN, USA3Department of Oncology, St Jude Children's Research Hospital, Memphis, TN, USA4Division of Hematology, Oncology and BMT, University of Minnesota, Minneapolis, MN, USA

5-5

Presence of chromosomally integrated HHV-6 in spermatozoaAngélique N Godet1, Marion Desmonet2, Gaëlle Cayrol3, Hannah Koubi2, Pascale Bonnafous1, Henri Agut2,Catherine Poirot3 ,Agnès Gautheret-Dejean1

1UPMC Univ Paris 06, ER1DETIV, Paris, France2Service de Virologie, Hôpitaux Universitaires Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France3Service d'Histologie à orientation Biologie de la Reproduction-CECOS, Hôpital Tenon, AP-HP, Paris,Université Pierre et Marie Curie,France

5-6

Investigation into germ-line transmitted chromosomally integratedHHV-6 and classical Hodgkin lymphomaAdam Bell1, Alice Gallagher1, Ruth Jarrett1

1MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland

5-7

Absence of chromosomally integrated HHV-6 detection in 91 parents offetus who died in uteroMustafa Al Jawhari1, Catherine Yardin2, Claire Laforest1, Sylvie Ranger-Rogez1

1Department of Microbiology, Pharmacy Faculty, Limoges, France2Cytogenetics Department, University Hospital, Limoges, France

5-8

Chromosomally integrated HHV-6 in healthy donor and patientstreated for haematological malignancy

Petr Hubacek1,2, Alena Hrdlickova1, Katerina Muzikova1, Martin Spacek3, Miroslav Zajac2, Petr Sedlacek1, PetrCetkovsky4, Jan Stary1

1Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University andMotol University Hospital, Prague, Czech Republic2Department of Medical Microbiology, Motol University Hospital, Prague, Czech Republic3Department of a Clinical Haematology, Third Faculty of Medicine, Charles University and University HospitalKralovske Vinohrady, Prague, Czech Republic4Department of Clinical Haematology, Institute of Haematology and Blood Transfusion, Prague, CzechRepublic

5-9

Analysis of HHV-6 TRS copy numbers in clinical isolates: Possibility ofsuperinfectionYuri Kato1, Masaru Ihira2, Yuki Higashimoto1, Yoshiki Kawamura3, Masahiro Ohashi3, Tetsushi Yoshikawa3

1Department of Clinical Laboratory, Fujita Health University Hospital, Toyoake, Japan2Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Japan3Department of Pediatrics, Fujita Health University School of Health Sciences, Toyoake, Japan

5-10

HHV-6 lytic and latent infection in a wide variety of tissuesLaura A Adamson1, Jacqueline A Hobbs1

1Departments of Psychiatry and Pediatrics, University of Florida College of Medicine, Gainesville, FL, USA

5-11

Beta-Herpesvirus in human periodontitis: Could it play a role?Ronaldo Thomasini1, Rafael Elias1, Oscar Bruna-Romero2, Antonio Teixeira1, Fabiana Pereira1, MauroTeixeira1

1Interdisciplinary Lab of Medical Investigation- Immunopharmacology Group, Department of Biochemistryand Immunology, Federal University of Minas Gerais, São Paulo, Brazil2Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, SantaCatarina, Brazil

5-12

Human betaherpesviruses in children undergoing cancer chemotherapyJohanna Goldfarb1, Nirica Borges1, Kate Gowans1, Debra Kohn2, Sarah Worley2, Belinda Yen-Lieberman2,Donna Lach1, Philip Pellett3

1Cleveland Clinic Children's Hospital, Cleveland, OH, USA2Cleveland Clinic, Cleveland, OH, USA3Wayne State University, Detroit, MI, USA

5-13

Diagnostics

Diagnosing HHV-6 encephalitis: Tools and algorithmsAgnès Gautheret-Dejean1, Reed Hardin2, Stéphanie Nguyen-quoc3, Francis Bolgert4, Cécile Henquell5, BrunoChanzy6, Marianne Giroux7, Hélène Prodanovic8, Pascale Bonnafous1, Henri Agut1

1UPMC Univ Paris 06, ER1DETIV, and Service of Virology, University Hospitals Pitié Salpêtrière - CharlesFoix, AP-HP, Paris, France2HHV-6 Foundation, Santa Barbara, CA, USA3Service of Clinical Hematology, University Hospitals Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France4Neurological Intensive Care Unit, Neurology Department, University Hospitals Pitié Salpêtrière - CharlesFoix, AP-HP, Paris, France5Laboratory of Medical Virology, University Hospital Centre of Clermont-Ferrand, Clermont-Ferrand, France6Laboratory, Hospital Centre from the Area of Annecy, Annecy, France7Departement of Neurology, EA2686, University Lille Nord of France, Lille, France8Service of Pneumology and Medical Intensive Care Unit, University Hospitals Pitié Salpêtrière - Charles Foix,AP-HP, Paris, France

6-1

Development of a digital PCR assay for detection of chromosomallyintegrated human herpesvirus-6Ruth Hall Sedlak1, Linda Cook1, Meei-Li Huang1, Keith R Jerome1,2

1Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA,USA2The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

6-2

Towards the standardisation of nucleic acid amplification technology(NAT)-based assays for human herpesvirus 6 (HHV-6)Jacqueline Fryer1

1Division of Virology, National Institute for Biological Standards and Control (NIBSC), Hertfordshire, EN, UK

6-3

Digital droplet pcr detection of HHV6a and HHV6bEmily Leibovitch1, Giovanna Brunetto1, Nyater Ngouth1, Steven Jacobson1

1Viral Immunology Section, NIH, Bethesda, MD, USA

6-4

Direct detection of HHV-6 DNA from serum by LAMP method usingnewly developed dry reagentMasaru Ihira1, Yuki Higashimoto2, Hiroko Sugiyama2, Yoshihiko Enomoto2Yoshiki Kawamura3, MasahiroOhashi3, Yoshizo Asano4, Tetsushi Yoshikawa3

1Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Japan2Department of Clinical Laboratory, Fujita Health University Hospital, Toyoake, Japan3Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan4Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

6-5

Non-Neurological Diseases

Chronic/relapsing lymphadenopathy associated with HHV-6B infection:A new benign clinico-pathologic entity occurring in immunocompetentindividualsMario Luppi1, Fabio Forghieri1, Patrizia Barozzi1, Leonardo Potenza1

1Department of Medical and Surgical Sciences, UNIMORE, AOU Policlinico, Modena, Italy

7-1

Virologic and immunologic evidence supporting an associationbetween HHV-6 and Hashimoto's thyroiditisElisabetta Caselli1, M Chiara Zatelli2, Roberta Rizzo1, Debora Martorelli3, Ettore Degli Uberti2, RiccardoDolcetti3, Dario Di Luca1

1Microbiology Unit, Department of Medical Sciences, University of Ferrara, Ferrara, Italy2Section of Endocrinology, Department of Medical Sciences, University of Ferrara, Ferrara, Italy3Cancer Bio-Immunotherapy Unit, CRO IRCCS, National Cancer Institute, Aviano (PN), Italy

7-2

Human herpesvirus 6 and parvovirus B19 co-infection and persistencein thyroid cancer and other thyroid diseasesLaura A Adamson1, Larry J Fowler2, Jacqueline A Hobbs1

1Departments of Psychiatry and Pediatrics, University of Florida College of Medicine, Gainesville, FL, USA2Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine,Gainesville, FL, USA

7-3

HHV-6 is present at the gut level and may be implicated in somediseasesMustafa Al Jawhari1, Laurent Mardivirin1, Denis Sautereau2, Sylvie Ranger-Rogez1

1Department of Microbiology, Pharmacy Faculty, Limoges, France2Gastroenterology Department, University Hospital, Limoges, France

7-4

Presence of HHV-6 and HHV-7 infection in thyroid lobes of patientswith thyroid gland diseasesSabine Gravelsina1, Zaiga Nora-Krukle1, Alina Sultanova1, Maksims Cistjakovs1, Agnija Roce1, SvetlanaChapenko1, Egils Cunskis2, Modra Murovska1

1Riga Stradins University, A. Kirchenstein Institute of Microbiology and Virology, Riga, Latvia2Riga Eastern Hospital, Clinic “Gaiļezers”, Riga, Latvia

7-5

Non-Neurological Diseases: Malignancy

HHV-6 & Hodgkin LymphomaDavid S. Hudnall11Yale University School of Medicine

8-1

HHV-6 in adult and pediatric brain tumorsE. Susan Amirian1, Deborah Marquez-Do1, Michael Scheurer1

1Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA

8-2

Human herpesvirus-6 latent infection in glioma patientsJing Chi1, Lingyun Li1, Bin Gu2, Chun Zhang1, Guangyong Peng3, Feng Zhou1, Yun Chen1, Weixing Hu4, KunYao1

1Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China2Department of Neurosurgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China3Division of Infectious Diseases, Allergy, and Immunology, Department of Internal Medicine, Saint LouisUniversity, MO, USA4Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China

8-3

HHV-6 antigen and HHV-6 DNA expression in sporadic adenomatouspolyps of the colonRaisa Loginov1, Leena Halme2, Johanna Arola3, Ulla Turunen4, Irmeli Lautenschlager1

1Department of Virology, Helsinki University Central Hospital (HUSLAB) and University of Helsinki, Finland2Department of Gastroenterologic Surgery, Helsinki University Central Hospital, Espoo, Finland3Department of Pathology, Helsinki University Central Hospital (HUSLAB) and University of Helsinki, Finland4Department of Medicine, Division of Gastroenterology, Helsinki University Central Hospital, Helsinki, Finland

8-4

Non-Neurological Diseases: DRESS, DIHS

Saliva polymerase chain reaction assay for detection and follow-up ofherpesvirus reactivation in patients with drug reaction with eosinophilia andsystemic symptoms (DRESS)Vincent Descamps1, Sylvie Ranger-Rogez2 and the French Study of Cutaneous Drug Adverse Reaction1Department of Dermatology, Department of Infectious Diseases, Bichat Claude Bernard Hospital, UniversitéParis-Est Créteil Val de Marne, Paris, France2Department of Microbiology, Pharmacy Faculty, Limoges, France

9-1

Drug Reaction with eosinophilia and systemic symptoms: A multiorgandrug adverse reaction mediated by an anti-viral T cell responsePhilippe Musette1

1Rouen University Hospital and INSERM U905, Rouen, France

9-2

Detection and longitudinal monitoring of herpesviruses in 14 patientswith drug reaction with eosinophilia and systemic symptoms comparedto 29 patients with non-severe drug-induced exanthemas: Aretrospective studyMaud Bollaert1, Hélène Jeulin2, Julie Waton1, Isabelle Gastin3, Philippe Trechot4, Christian Rabaut5, Jean-LucSchmutz1, Annick Barbaud1

1Department of Dermatology, batiment des specialités médicales, Brabois Hospital, University Hospital ofNancy, Lorraine University, Nancy, France2Laboratoire de Virologie, University Hospital of Nancy, Lorraine University, Nancy, France3NSERM 954Unit, Lorraine University, Nancy, France4Pharmacology department, Central Hospital, University Hospital of Nancy, Lorraine University, Nancy,France5Department of Infectious and Tropical Diseases,batiment des specialites, Brabois Hospital, UniversityHospital of Nancy, Lorraine University, Nancy, France

9-3

Human herpesvirus 6 reactivation in trichloroethylene-inducedhypersensitivity dermatitisMichihiro Kamijima1, Hailan Wang2, Osamu Yamanoshita3, Yuki Ito1, Tetsushi Yoshikawa4, Laiyu Li2, HanlinHuang2, Tamie Nakajima5

1Department of Occupational and Environmental Health, Nagoya City University Graduate School of MedicalSciences, Nagoya, Japan2Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China3Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Japan4Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan5Nagoya University Graduate School of Medicine, Nagoya, Japan

9-4

Drug-induced hypersensitivity is enhanced by human herpes virus 6reactivationManuela Neuman1, Kristi Mckinen2, Radu Nanau1, Vincent Kong1, George Moussa3, Tony Mazulli3, LawrenceCohen4

1In Vitro Drug Safety and Biotechnology, Department of Pharmacology and Toxicology, University ofToronto, Canada2US Military3Department of Microbiology, Mount Sinai Hospital, University of Toronto, Canada4Department of Gastroenterology, SHSC, and Department of Medicine, University of Toronto, Canada

9-5

HHV-6 reactivation in a severe interstitial pneumopathy as part of anallopurinol induced DRESSBaptiste Balanca1, Camille Chassin2, Vincent Cadiergue1, Helene Baudrand2, Pierre Louis Blanc2

1Service de Reanimation Polyvalente, Centre Hospitalier, Annonay, France2Service de Medecine D, Centre Hospitalier, Annonay, France

9-6

Transplant Reactivation

Overview of HHV-6 reactivation in HSCTMichael Boeckh1

1Clinical Research, Vaccine and Infectious Disease , Fred Hutchinson Cancer Research Center, University OfWashington, USA

10-1

Human herpesvirus 6 infections in transplantationRaymund R Razonable1

1Division of Infectious Diseases and the William J von Liebig Transplant Center, Mayo Clinic, Rochester, MN,USA

10-2

Comparison of HHV-6, CMV, EBV and adenovirus (ADV) infectionsincidences after umbilical cord blood VS PBSC reduced-intensityconditioning allogeneic stem cell transplantation in 66 adults: Aprospective studyMarina Illiaquer1, Berthe-Marie Imbert-Marcille1, Lucie Planche3, Claire Le Houerou2, Celine Bressollette-Bodin1, Thierry Guillaume2, Mohamad Mohty2, Patrice Chevallier2

1Service de virologie, CHU Nantes, Nantes, France; UPRES-EA4271, PRES L'UNAM, Universite de Nantes,France2Service d'Hematologie Clinique, CHU Nantes; Universite de Nantes, France3Cellules de Promotion de la Recherche Clinique, CHU Nantes, Nantes, France

10-3

Interpreting virological results in a recipient of a ciHHV-6 livertransplantPascale Bonnafous1, Flor Munoz2, Ryan Himes2, Karen Eldin2, Agnes Gautheret-Dejean1, Henri Agut1

1Université Pierre et Marie Curie, Paris, France2Departments of Pediatrics, Molecular Virology and Microbiology, and Pathology and Immunology, TexasChildren’s Hospital and Baylor College of Medicine, Houston, TX, USA

10-4

A prospective study of HHV-6B infection in the kidney allograftIlkka Helanterä1, Irmeli Lautenschlager2, Anne Räisänen-Sokolowski3, Petri Koskinen1

1Department of Medicine, Division of Nephrology, Helsinki University Central Hospital, Helsinki, Finland2Department of Virology, Helsinki University Central Hospital, Helsinki, Finland3Department of Pathology, Helsinki University Central Hospital, Helsinki, Finland

10-5

Higher HHV-6 reactivation after umbilical cord blood allo-SCT is notrelated to HHV-6 cell receptor CD46 expressionPatrice Chevallier1, Nelly Robillard2, Marina Illiaquer3, Mohamad Mohty1, Celine Bressollette-Bodin3,Veronique Stocco4, Lucie Planche5, Berthe-Marie Imbert-Marcille3

1Hematology Department, CHU Hotel-Dieu and INSERM U892, Nantes, France2Biology Department, CHU Hotel-Dieu, Nantes, France3Virology Department, CHU Hotel-Dieu, Nantes, France4Unite d'ingénierie cellulaire, EFS Pays de Loire, Nantes, France5Cellule de Promotion à la recherche clinique, CHU Hotel-Dieu, Nantes, France

10-6

Human Herpesvirus-6 (HHV-6) myopericarditis and arthritis afterdouble umbilical cord blood reduced intensity conditioning allogeneicstem-cells transplantation: A case reportMarina Illiaquer1, Benoit Tessoulin2, Fanny Rialland3, Berthe-Marie Imbert-Marcille1, Celine Bressollette-Bodin1, Mohamad Mothy2, Patrice Chevallier2

1Service de virologie, CHU Nantes, Nantes, France, UPRES-EA4271, PRES L'UNAM, Universite de Nantes,France2Service d'Hematologie Clinique, CHU Nantes, Universite de Nantes, France3Service d'Oncologie pediatrique, CHU Nantes, Universite de Nantes, France

10-7

Human Herpesvirus 6 & 7 (HHV-6 AND HHV-7) antigenemia afterhematopoietic stem cell transplantation (HSCT) in Brazilian patientsand its relationship to active human cytomegalovirus (HCMV)Andréia Cristina Gulin1, Priscila Navas Pancielli1, Afonso Celso Vigorito2, Carolina Costa Lima2, Renata MariaBorges Peres1,Claudio Lucio Rossi3, Sandra Cecília Botelho Costa1, Sandra Helena Alves Bonon1

1Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - UNICAMP,Campinas, SP, Brazil2Bone Marrow Transplant Unit, Hemocenter, University of Campinas - UNICAMP, Campinas, SP, Brazil3Department of Internal Medicine, Faculty of Clinical Pathology, University of Campinas - UNICAMP,Campinas, SP, Brazil

10-8

The long-time outcome of the patients transplanted due to acute liverfailure (ALF) with hepatic human herpesvirus-6B (HHV-6B) infectionMaiju Härmä1, Krister Höckerstedt2, Irmeli Lautenschlager3

1Departments of Surgery and Virology, Department of Plastic Surgery, Helsinki University Hospital andUniversity of Helsinki, Helsinki, Finland1Department of Surgery, Transplantation and Liver Surgery Clinic, Helsinki University Hospital, Helsinki,Finland1Department of Virology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

10-9

Monitoring and quantification of human herpesvirus 6 after livertransplantationAna Carolina Guardia2, Arlete Milan2, Renan Colombari2, Joseane Morari3, Raquel Stucchi1,2, Célia Pavam3,Ana Maria Sampaio2, Ilka Boin2

10-10

Treatments

Overview of therapeutic strategies against HHV-6Henri Agut1

1ER1 DETIV UPMC, Department of Virology, Hôpital La Pitié-Salpêtrière, Paris, France

11-2

Immunotherapeutic strategies to prevent and treat human herpesvirus (HHV) 6 reactivation post allogeneic stem cell transplantAnn Leen1

1Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, and Texas Children'sHospital, Houston, TX, USA

11-3

Prophylactic foscarnet and suppression of pre-engraftment immunereactions are essential to overcome the development of HHV-6encephalitis after cord blood transplantationYuki Asano-Mori1, Naoyuki Uchida1, Kazuya Ishiwata1, Hisashi Yamamoto1, Koji Izutsu1, Shuichi Taniguchi11Department of Hematology, Toranomon Hospital, Tokyo, Japan

11-4

Therapeutic potential of cidofovir (HPMPC, VISTIDE) for the treatmentof presumed HHV-6 and/or CMV infections in severely ill patientsdiagnosed with chronic fatigue syndrome/myalgic encephalomyelitisGunnar Gottschalk1, Isabel Barao2, Daniel Peterson1

1Sierra Internal Medicine, Incline Village, NV, USA2University of Nevada, Reno, NV, USA

11-5

HHV-6 U69 Kinase phosphorylates methylenecyclopropane nucleosidesMBX 2168 and MBX 1616 to their monophosphatesGloria Komazin-Meredith1, Steven Cardinale1, John Williams1, Norton Peet1, Mark Prichard2, Terry Bowlin1

1Microbiotix, Inc., Worcester, MA, USA2University of Alabama School of Medicine, Birmingham, AL, USA

11-6

Identification of HHV-6 Antigenic Peptides for Detection of HHV6Specific AntibodiesNaomi Lee1, Emily Leibovitch1, J Mauricio Calvo-Calle2, Lawrence J Stern2, Steven Jacobson1

1Viral Immunology Section, NIH, Bethesda, MD, USA2Department of Pathology, University of Massachusetts, Worcester, MA, USA

11-7

First therapeutic use of artesunate in treatment of human herpesvirus6B myocarditis in a human childNina Hakacova1, Karin Klingel2, Reinhard Kandolf2, Elin Engdahl3, Anna Fogdell-Hahn3, Thomas Higgins1

1Department of Pediatric Cardiology, Skane University Hospital, Lund, Sweden2Department of Molecular Pathology, University Hospital Tübingen, Germany3Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

11-8

HHV-6 in CNS Disease

HHV-6 in CNS Disease OverviewSteven Jacobson1

1Viral Immunology Section, NIH, Bethesda, MD, USA

12-1

HHV-7 and neurological disease in children and adultsKatherine N. Ward1

1Department of Infection, University College London, London, UK

12-2

Prevalence of HHV-6A, HHV-6B and HHV-7 in CSF specimens from HIVinfected adult inpatients admitted with a range of CNS disorders in alarge referral hospital in sub-Saharan AfricaMatthew Bates1,5, Omar Siddiqi 2,3, John Tembo1, P Kaonga 3, Masharip Atadzhanov3, Kunda Musonda4,Ursula Gompels4, Shabir Lakhi3, Alimuddin Zumla5, Igor Koralnik2

1University of Zambia and University College London Medical School, Research & Training Programme andHerpeZ, Lusaka, Zambia2Division of Neurovirology, Department of Neurology and the Division of Viral Pathogenesis, Beth IsraelDeaconess Medical Center, Harvard Medical School, Boston, MA, USA3Department of Internal Medicine, University Teaching Hospital, Lusaka, Zambia4Pathogen Molecular Biology Unit, Department of Infectious Tropical Diseases, London School of Hygieneand Tropical Medicine, UK5Division of Infection and Immunity, University College London, UK

12-3

Co-infection of HHV-6 and HHV-7 in patients with myalgicencephalomyelitis/chronic fatigue syndromeSanta Rasa1, Svetlana Chapenko1, Zaiga Nora-Krukle1, Angelika Krumina2, Ludmila Viksna2, ModraMurovska1,1August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Riga, Latvia2Department of Infectology and Dermatology, Riga Stradins University, Riga, Latvia

12-4

Human herpesvirus 6 and 7 infections in cerebrospinal fluid of patientswith encephalitis and neurological diseasesCarla Aparecida Tavares Rimério1, Daniela Corte Parola1, Murilo Queiroz de Almeida Bonatelli1, Renato Souzade Oliveira1,Anamarli Nucci2, Sandra Cecília Botelho Costa1, Sandra Helena Alves Bonon1

1Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - UNICAMP,Campinas, SP, Brazil2Department of Neurology, Faculty of Medical Sciences, University of Campinas - UNICAMP, Campinas, SP,Brazil

12-5

HHV-6 co-infection in a patient with fulminant progressive multifocalleukoencephalopathy of the brain and spinal cordGloria Von Geldern1, Sara Kranick1, Gene Major1, Steve Jacobson1, Martha Quezado1, Irini Sereti1, Cliff Lane1,Avindra Nath1

1National Institutes of Health, Bethesda, MD, USA

12-6

HHV-6 in CNS Disease: Animal models

Using animal models to study virus and neurological diseaseSusan V. Westmoreland1

1Division of Comparative Pathology, Harvard Medical School, New England Primate Research Center, Maine,USA

13-1

Intranasal HHV-6A infection accelerates EAE in the common marmosetEmily Leibovitch1, Pietro Maggi1, Sheila Cummings Macri2, Giovanna Brunetto1, Susan Westmoreland2,Afonso Silva1,Daniel S Reich1, Steven Jacobson1

1National Institutes of Health, NINDS, Bethesda, MD, USA2New England Primate Research Center, Harvard Medical School, Boston, MA, USA

13-2

HHV-6A infection induces neuroinflammation in CD46-transgenic miceJosephine Reynaud1, Jean-François Jegou1, Jeremy Welsch1, Branka Horvat1

1International Center for Infectiology Research, INSERM U1111, CNRS UMR5308, Ecole Normale Supérieurede Lyon, University of Lyon 1, Lyon, France

13-3

Humanized mice as a novel model to study human herpesvirus 6tropism and infectionAnne Tanner1, Stephanie Carlson1, Wittnee Decottignies1, Stephen Taylor1, Bradford Berges1

1Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA

13-4

HHV-6 in CNS Disease: Multiple Sclerosis

Serological response against hhv-6 in multiple sclerosis patients inrelation to treatment and clinical activityIsabel Ortega-Madueño1, Marta Garcia-Montojo1, Maria Inmaculada Dominguez-Mozo1, Ignacio Casanova-Peño2, Ana Maria Arias-Leal1, Maria Angel Garcia-Martinez1, Rafael Arroyo2, Roberto Alvarez-Lafuente1

1Laboratorio de Investigacion de Esclerosis Múltiple, Ciudad Universitaria, Madrid, Spain2Unidad de Esclerosis Múltiple, Servicio de Neurologia, Hospital Clínico San Carlos, Madrid, Spain

14-1

Herpesvirus-6 antigens recognized by IgG antibodies present in CSF ofmultiple sclerosis patientsRaquel Alenda1, Roberto Álvarez-Lafuente2, José Carlos Álvarez-Cermeño2, Luisa Maria Villar3

1Departamento de Producción Animal, E.T.S.I. Agrónomos-Universidad Politécnica de Madrid, Madrid, Spain2Laboratorio de Investigacion de Esclerosis Múltiple, Ciudad Universitaria, Madrid, Spain3Facultativo del Servicio de Inmunología, Hospital Ramon y Cajal, Madrid, Spain

14-2

Multiple sclerosis and viral risk factors: can we target latent infection?Ute Meier1

1Blizard Institute, Neuroimmunology Group, Department of Neuroscience and Trauma, Queen MaryUniversity of London, Barts and The London School of Medicine and Dentistry, London, UK

14-3

HHV-6 infection in multiple sclerosis: A trigger for autoimmunity?Francesco Broccolo1, Sabrina Matà2, Vittorio Martinelli3, Giulia Cassina4, Davide De Battista4, Marta Radaelli3,Tiziana Biagioli2, Lisa Fusetti5, Luca Ceccherini-Nelli5, Paolo Lusso6, Roberto Furlan3, Mauro S Malnati41Department of Clinical Medicine, Prevention and Biotechnology, University of Milano-Bicocca, Milan, Italy2Department of Neurological and Psychiatric Sciences, AUO Careggi, Florence, Italy3Department of Neurology and Unit of Neuroimmunology, Division of Neurological Sciences OSR ScientificInstitute, Milan, Italy4Unit of Human Virology, Division of Immunology, Transplantation and Infectious Diseases, OSR ScientificInstitute, Milan, Italy5Department of Experimental Pathology, B.M.I.E. University of Pisa, Pisa, Italy6Section of Viral Pathogenesis, Laboratory of Immunoregulation NIAID, NIH, Bethesda, MD, USA

14-4

HHV-6 antibody titers and the association with subsequent relapse,disability and annual change in disability in people with MultipleSclerosisSteve Simpson, Jr1, Bruce Taylor1, Jacqueline Burrows2, Scott Burrows2, Dominic E Dwyer3, Janette Taylor3,Anne-Louise Ponsonby4, Leigh Blizzard1, Terence Dwyer4, Fotini Pittas5, Ingrid van der Mei11Menzies Research Institute Tasmania, University of Tasmania, Hobart, Tasmania2Queensland Institute for Medical Research, Brisbane, Australia3Westmead Millennium Institute and ICPMR, Westmead Hospital, Westmead, Australia4Murdoch Children’s Research Institute, Royal Children’s Hospital, Parkville, Australia5School of Medicine, University of Tasmania, Hobart, Tasmania

14-5

Prevalence of human herpesvirus U94/REP antibodies and DNA inTunisian multiple sclerosis patientsNadia Ben Fredj1, Antonella Rotola2, Faten Nefzi1, Mahbouba Frih-Ayed3, Dario Di Luca2, Mahjoub Aouni1,Elisabetta Caselli21Laboratory of Transmissible Diseases and Biological Active substances, LR99-ES27, Faculty of Pharmacy,University of Monastir, Monastir, Tunisia2Department of Medical Sciences, Microbiology Unit, University of Ferrara, Ferrara, Italy3Department of Neurology, Fattouma Bourguiba University Hospital, Monastir, Tunisia

14-6

Herpesvirus reactive oligoclonal bands in multiple sclerosis CSF andbrain tissueJussi Oskari Virtanen1, Emily Leibovitch1, Jillian Wohler1, Kaylan Fenton1, Daniel S Reich1, Steven Jacobson1

1Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA

14-7

HHV6 IgG response and genetic factor rs11154801 associate withrelapse rate in childrenJennifer Graves1, Lisa Barcellos2, Lauren Krupp3, Anita L Belman3, Judith A James4, Emmanuelle Waubant1

1Neurology, University of California, San Francisco, CA, USA2Public Health, Genetic Epidemiology, University of California, Berkeley, CA, USA3Neurology, Stony Brook University Medical Center, Stony Brook, NY, USA4Rheumatology, Oklahoma Medical Research Foundation, University of Oklahoma, Norman, OK, USA

14-8

No difference in serological response against HHV-6 between MSpatients and controlsElin Engdahl1, Rasmus Gustafsson1, Ryan Ramanujam1, Emilie Sundqvist1, Tomas Olsson1, Jan Hillert1, IngridKockum1, Anna Fogdell-Hahn1

1Karolinska Institutet, Department of Clinical Neuroscience, Stockholm, Sweden

14-9

HHV-6 in CNS Disease: Epilepsy & Seizures

Overview of HHV6B & 7 in seizures & febrile status epilepticusLeon Epstein1

1Department of Neurology, Chicago Children's Memorial Hospital, Northwestern University Feinberg Schoolof Medicine, Chicago, IL

15-1

Cytokine and chemokine response in the blood and cerebrospinal fluidof human herpesvirus 6B associated acute encephalopathy withbiphasic seizures and late reduced diffusionYoshiki Kawamura1, Yuri Kato1, Masahiro Ohashi1, Masaru Ihira2, Tetsushi Yoshikawa1

1Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan2Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Japan

15-2

Large scale epilepsy surgery tissue screening reveals HHV-6B DNA in ahigh number of patients with distinct initial precipitating insultsLaura Esposito1, Alexander Grote2, Jan F Drexler3, Guido Widman4, Susanne Schoch1, Albert J Becker1, PittNiehusmann1

1Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany2Department of Neurosurgery, University of Bonn Medical Center, Bonn, Germany3Department of Virology, University of Bonn Medical Center, Bonn, Germany4Department of Epileptology, University of Bonn Medical Center, Bonn, Germany

15-3

Human Herpesvirus-7 (HHV-7) in brain tissue from adults withintractable epilepsy: a pathological role in some cases of hippocampalsclerosis?Jin-Mei Li1, Cheng Huang1, Wei Wang1, Qiao Zhou2, Josemir W. Sander3,4, Dong Zhou1

1Department of Neurology, West China Hospital of Sichuan University, Chengdu, China2Department of Pathology, West China Hospital of Sichuan University, Chengdu, China3UCL Institute of Neurology, Queen Square, London, UK4SEIN – Stichting Epilepsie Instellingen Nederland, Heemstede, Netherlands

15-4