identification of enterobacteriaceae
DESCRIPTION
Islamic University of Gaza Faculty of science Medical technology department. الجامعة الاسلامية بغزه كلية العلوم قسم التحاليل الطبية. IDENTIFICATION OF ENTEROBACTERIACEAE. Prepared by: Abdullah Ali. Supervised by: Dr.Abdellraof el-Manama Dr.Nedal Fayyad. - PowerPoint PPT PresentationTRANSCRIPT
IDENTIFICATION OFIDENTIFICATION OFENTEROBACTERIACEAEENTEROBACTERIACEAE
Prepared byPrepared by::Abdullah AliAbdullah Ali
Supervised by:Dr.Abdellraof el-ManamaDr.Nedal Fayyad
بغزه االسالمية الجامعةالعلوم كلية
الطبية التحاليل قسم
Islamic University of GazaFaculty of scienceMedical technology department
Characteristics of EnterobacteriaceaeMembers of the Enterobacteriaceae are Gram-negative, straight rods, some of which are motile. Most species grow well at 37°C,
although some species grow better at 25 - 30°C. They are facultativelyanaerobic, oxidase-negative and catalase-positive (except Shigella dysenteriae type 1). They are distributed worldwide and may be
found in soil, water, plants and animals.
Common genera of the family Enterobacteriaceae
1) Citrobacter species2 )Enterobacter species
3 )Escherichia species4 )Hafnia alvei
5 )Klebsiella species6 )Shigella species
7) Morganella morganii
8 )Proteus species9 )Providencia
species10 )Salmonella
species11 )Serratia species12 )Yersinia species
Incidence of Enterobacteriaceae Associated with Bacteremia
EscherichiaEscherichia
Escherichia coliEscherichia coli
SalmonellaSalmonella
EnterobacterEnterobacter
Enterobacter aurogeouns Enterobacter sakazakii
ShigellaShigella
CitrobacterCitrobacter
Can they ferment lactose?
Can they use citric acid as their sole carbon source?
Can they use citric acid as their sole carbon source?
Do they produce acetoin?
EscherichiaSalmonellaShigella
(Yes)
(Yes)
(Yes)
(Yes)
(No)
(No)(No)
(No)
Citrobacter Enterobacter
Can they ferment lactose?
Can they use citric acid as their sole carbon source?
Tests:
From the previous slide we observe that we need to do the following tests:
1)Lactose fermentation
2)Citrate test
3)Voges-proskauer test
Citratetest
Some disease causing by Some disease causing by Enterobacteriaceae speciesEnterobacteriaceae species
speciesdisease
E. coliE. coli.diarrhea, invasive disease
SalmonellaSalmonella•diarrhea, septicemia, pneumonia
ShigellaShigella .dysentery
ConclusionFamily Enterobacteriaceae have the followingcharacteristic:
1)Gram-negative rod2)Non-sporeforming bacteria
3)Facultative anaerobe4)Motile by peritritchous flagella
5)Oxidase negative6)Non acid fast bacteria
7)Produce nitrate reductase
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