iii respiratory chain ( 呼吸链 ) 1. concept : respiratory chain, or electron-transport chain (...
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III Respiratory chain (呼吸链 )
1. Concept: Respiratory chain, or electron-transport chain (电子传递链 ), is one of the redox chains in organism.
redox chain (氧化还原链 ):Metabolic pathway consist of (oxidative) metabolic intermediates, H donors, transfers, H final acceptor and relevant enzyme system.
By LI Dehong
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III Respiratory chain (呼吸链 )
1. Concept: (Cont.)
Redox chians include 5 chains: respiratory chain,
microsomal oxidative system (微粒体氧化体系 ), peroxidase system (过氧化物酶氧化体系 ), ascorbate oxidative system (抗坏血酸氧化体系 ) and polyp
henol oxidase system (多酚氧化酶体系 )(in plants).
By LI Dehong
We’d focus on Respiratory chain since it can produce ATP.?
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1. Concept (Cont.)
III Respiratory chain
A2e + BA +B 2e
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1. Concept: (Cont.)
• H final acceptor of respiratory chain: O2
product: H2O
• Transfers of respiratory chain: H, H+, e.
• Classification of respiratory chain:
Respiratory chains can be classified into 2 kinds by the initial H donor <co-factor>.
NADH+H+ respiratory chain
FADH2 respiratory chain
III Respiratory chain
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2. Components of respiratory chain
1). Intracellular site \ location
III Respiratory chain
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2. Components of respiratory chain (Cont.)
III Respiratory chain
Cristae: 嵴
Matrix: 基质
Movie 1
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2). 5 components form 4 enzyme complex and relevant co-factors:
NADH-Q reductase: coenzyme NAD+ \ NADP+ (Vt PP, Vt B5)
Succinate-Q reductase: prosthetic group \ FAD (Vt B2)
FeS Protein: (transfer one electron)
CoQ: The only non-protein component in respiratory chain. It occurs in organism widely. (ubiquinone, 泛醌 )
Role as H transfer: quinone (醌 ) 2Hhydroxybenzene (酚 ) Cytochrome proteins
(see also next slide)
2. Components of respiratory chain (Cont.)
III Respiratory chain
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2). 5 components form 4 enzyme complex and relevant co-factors:
NADH-Q reductase: co-enzyme NAD+ \ NADP+ (Vt PP, Vt B5)
2. Components of respiratory chain (Cont.)
III Respiratory chain
Complex I
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Succinate-Q reductase) [琥珀酸 -Q 还原酶 ]:
prosthetic group: FAD (Vt B2)
III Respiratory chain
Complex II
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III Respiratory chain
NADH dehydrogenase (NADH-Q reductase)
NADH+H+
NAD+
FMN
FMNH2 Fe3+£ S
Fe2+£ S CoQ
CoQH2
Succinate[琥珀酸 ]dehydrogenase (Succinate-Q reductase)
succinate
Fumarate
FAD
FADH2 Fe3+£ S
Fe2+£ S CoQ
CoQH2
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III Respiratory chain
FeS protien:
transfer 1 electron
Fe3+ + e Fe2+
Cys = cysteine
-SH = sulfhydryl group
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CoQ: The only non-protein component in respiratory chain. It occurs in organism widely. (ubiquinone, 泛醌 )
Role as H transfer: (醌 ) 2H (酚 ) quinone hydroxybenzene
III Respiratory chain
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Cytochromes (细胞色素蛋白 ): 1) Transfer one electron;
2) Prosthetic group: hemachrome (血红素 , 含铁卟啉 <Fe-porphyrin>)
(Fig. 7-4, p151)
2. Components of repiratory chain (Cont.)
III Respiratory chain
Cyt b Cyt c1→Cyt c→Cyt a→Cyt a3→ O2 Fe-S
Fe3+ + e Fe2+
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III Respiratory chain
Structure of hemachrome & its connection with protein
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III Respiratory chain
Structure of cyt c & cyt c1
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III Respiratory chain
Absorbance of cytochromes
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o 5 kinds of cytochromes: cyt b, cyt c1, cyt c, cyt aa3.
The 5 kinds cyt all contain Fe (cyt a3 contains also Cu+).
They bind tightly to mitochondrion except cyt c.
o To form 2 enzymes:
CoQH2-cyt c reductase: cyt b, FeS, cyt c.
function: catalyze electron CoQH2 cyt c
Cyt c oxidase: cyt aa3.
function: catalyze electron cyt c cyt aa3
2. Components of respiratory chain (Cont.)
III Respiratory chain
\\Complex IV
\\Complex III
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3). Order /Sequence of transporters in respiratory chain
SNADHFMNFeSCoQ
SuccinateFAD
cyt bFeScyt c1 cyt ccyt aa3O2Complex II
Complex III
Complex I
III Respiratory chain
Complex IV
(The components are sequenced by the redox potentials [氧化还原电位 ] of the components of respiratory chain)
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• Respiratory Chain (see also Fig 6.6, p189)
III Respiratory chain
I
II III IV
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III Respiratory chain
Respiratory Complex I & II
Movie 2
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III Respiratory chain
Movie 3
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III Respiratory chain
Movie 4
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III Respiratory chain
Let’s go back to
the very beginning of this topic:
Where and how does ATP be produced?
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3 steps can produce ATP: (3 enzyme complex)
NADH CoQ (The Potential of NADH is -3.2 V)
CoQ cyt ccyt c O2
potential of respiratory chain lowerhigher (negativepositive)
The lower the reductive potential, the easy the electron losing.
3. Product of ATP
(I)(III)(IV)
III Respiratory chain
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En
ergy
III Respiratory chain
potential of respiratory chainnegativepositive(负正 )
lower higher [能量 : 高低 ]
The lower the reductive potential, the easy the electron losing.
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3. Product of ATP (Cont.)
III Respiratory chain