introducing cpa200 and cpa300 punching systems
DESCRIPTION
Card Processing AutomationTRANSCRIPT
1 The world leader in serving science
Next Generation Punching Systems for Reference DNA Samples 2014
Introducing CPA200 & CPA300
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Collection Punching Amplification Detection Analysis
3500 and 3500xL Genetic
Analyzers
GeneMapper® ID-X expert
system software
Direct PCR Amplification Kit
NUCLEIC-CARD™ Framed Cards
GeneAmp® PCR System 9700 Cycler Veriti® 96-Well Thermal Cycler
BSD® semi-automated punching systems
BSD® Automated punching systems
NUCLEIC-CARD™ Unframed Cards
Direct Amplification Workflow for DNA Databasing
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Why a Punching System?
Increase Throughput
Improve Lab
Efficiency
Minimize Operator
Errors
Enhance Chain-of- Custody Tracking
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Update on BSD® Punchers by Luminex
• On April 28, 2014 Luminex communicated to Life Technologies the plan to cease the operations of BSD and the manufacture of BSD® punchers
• On the same date, Luminex announced they would no longer accept any BSD® Puncher orders
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Life Technologies and BSD® Punchers
• Life Technologies has been a distributor of BSD® Punchers in the forensic market since the end of 2009
• Life Technologies recognizes the importance of service and support for deployed BSD® instruments
• Life Technologies plans to continue to offer its standard comprehensive service plan for the BSD® punchers until June 30th, 2016
• As a result of the Luminex decision, Life Technologies may need to offer more limited service
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Life Technologies & Copan
• Life Technologies started distributing Copan sample collection devices for forensic applications including the NUCLEIC-CARD™ system in 2012
• Life Technologies and Copan signed a distribution agreement in June 2014 to distribute the CPA technology developed by Copan
• Life Technologies is now the global exclusive distributor of CPA instruments in the human identification market
• Life Technologies will provide service and support for installed CPA instruments
NUCLEIC-CARD™ System FLOQSwabsTM Devices
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Introducing CPA200 and CPA300
CPA2001 Semi-automated puncher
CPA3001 Fully-automated puncher & liquid handler
1. Card Processing Automation
PC Operational Box Operational Box PC
Support Box
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CPA200 – General Specs
CPA2001 Semi-automated puncher
1. Card Processing Automation
Dimensions 610mm (L) x 545mm (W) x 675mm (H)
Weight 72 kg Peripherals Touchscreen,
keyboard, mouse, USB (x2)
Network Ethernet 1 Gbit/sec
Exportable files .pdf & .csv files
Available Late Summer 2014!
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CPA200 – Supported Cards
Optimized for NUCLEIC-CARD™ with Cleaning Area
• Validated punch size: 1.2 mm
• Additional punch sizes are currently under consideration
• Protocol with “cleaning punch” available
CPA200 works with sample collection systems routinely used in forensic laboratories: • NUCLEIC-CARD™ (regular and custom) • FTA® Cards (Whatman) • Buccal DNA Collector (Bode)
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CPA200 – Punch Quality
CPA200 includes: • Ionizer – to neutralize the electrostatic charges on the
puncher tool to ensure detachment of paper disks and release in the correct well
• Dust Remover – to remove any card debris from the puncher tool to ensure excellent cleaning conditions and optimal cutting quality
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Intuitive and User-Friendly User Interface
Real-time updates on plate status
Camera control of each well before and
after punching
Event List
Workflow
User Protocol Status
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CPA200 – Cross-Contamination Study
• Objective: Assess well cross-contamination when preparing a 96-well plate with CPA200
• Method: Collect samples from 23 donors using FLOQSwabs™ Regular Transfer samples onto 1 spot NUCLEIC-CARD™ COLOR, 4 spots
NUCLEIC-CARD™ COLOR, FTA® cards and Bokumbio cards. Cards were dried at RT for one hour Add 15 µL GlobalFiler® Express Reaction Mix1 to each well of the 96-
well plate Punch one 1.2 mm disc in each well of the plate followed by one
cleaning strike between punches Seal the plate and centrifuge it at 300 rpm for 20 seconds Amplify the samples using a GeneAmp® PCR System 9700
1. For Forensic or Paternity Use Only
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CPA200 – Plate Set Up 1 2 3 4 5 6 7 8 9 10 11 12
A NC1 NP8 NC9 NP15 NC17 NP22 NP29 NC29 NP37 NC36 NP44
B NP1 NC2 NC10 NP16 NC18 NP23 NC25 NP33 NC32 NP41 NC40
C NP2 NC3 NP9 NC11 NC19 NP24 NP30 NC30 NP38 NC37 NP45
D NP3 NC4 NP10 NC12 NP17 NC20 NC26 NP34 NC33 NP42 NC41
E NP4 NC5 NP11 NC13 NP18 NC21 NP25 NP31 NP39 NC38 NP46
F NP5 NC6 NP12 NC14 NP19 NC22 NP26 NC27 NP35 NC34 NC42
G NP6 NC7 NP13 NC15 NP20 NC23 NP27 NP32 NC31 NP40 NC39 POS
H NP7 NC8 NP14 NC16 NP21 NC24 NP28 NC28 NP36 NC35 NP43 NEG
Legend: • NP: Non-Probative samples • NC: Negative Control samples – Blank punches used as substrate control • NEG: Negative Control DNA well - No punches added to this well • POS: Control DNA well – No punches added to this well
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CPA200 – Electropherograms
• Representative electropherogram generated from NP well
• Full STR profile generated
• Representative electropherogram generated from NC well
• No STR profile generated
Data generated using Applied Biosystems® 3130 Genetic Analyzer
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CPA200 – Key Findings
• Good punch quality with different card types • No instances of incorrect barcode reading • No disc losses • Consistent release of the paper discs at the bottom of the
well as confirmed by the real-time feedback from the camera • No instances of cross-contamination • Full concordance between STR profile and expected profile
from the donor1 • Very user-friendly software
1. Samples from each donor were spotted on two cards. Each card was punched so that the discs associated with each donor randomly ended up in two different plate wells
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CPA300 – General Specs
CPA3001 Fully-automated puncher
1. Card Processing Automation 2. Operational box
Dimensions 1160mm (L) x 760mm (W) x 1005mm (H)2
Weight 270 kg Peripherals Touchscreen,
keyboard, mouse, USB (x2)
Network Ethernet 1Gbit/sec
Exportable files .pdf & .csv files
Available early Fall 2014!
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CPA300 – Supported Cards
CPA300 works with framed cards: • 51 mm x 51 mm (single collection area) • 85 mm x 53 mm (two collection areas)
Validated punch size: 1.2 mm
NUCLEIC-CARD™ Collection Device
NUCLEIC-CARD™ with Cleaning Area
Whatman EasiCollect
Custom Framed Cards
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CPA300 – Operational Box Overview
Sealing Unit
Tips Station
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CPA300 – Operational Box Overview
Storage
Punching Unit
Scanning Area Plate Gripper Card Gripper
Pipetting Unit
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CPA300 – Punching Unit
• Puncher has two main functions: generates a 1.2 mm disk that
is safely released in the 96 well plate performs the cleaning
procedure. The cleaning disk(s) are released in a separate bin
• Ionizer removes the electrostatic
charges
• Dust Remover removes card debris
Two cards per minute WITH
cleaning punch
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CPA300 – Plate and Card Grippers
• Plate gripper enables the movement of the plate during the punching and liquid handling procedures
CPA300 can accommodate up to three 96-well plates
Bin to collect cleaning punches
• Card gripper transports the card from and to the card rack to enable the punching procedure
CPA300 can accommodate up to four card racks1
1. Each rack fits up to 75 cards
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Sealing Unit
• Plates are automatically sealed at the end of the liquid handling procedure
Reduces risks of cross-contamination Helps prevent reagent
evaporation PCR-ready plate for better
efficiency
Full cycle: Approx. one hour per plate1
1. Includes punching, cleaning, liquid handling and sealing
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Collection Punching Amplification Detection Analysis
3500 and 3500xL Genetic
Analyzers
GeneMapper® ID-X expert
system software
Direct Amplification Workflow for DNA Databasing
Direct PCR Amplification Kit
NUCLEIC-CARD™ Framed Cards
GeneAmp® PCR System 9700 Cycler Veriti® 96-Well Thermal Cycler CPA200
CPA300
NUCLEIC-CARD™ Unframed Cards
28 The world leader in serving science
Please contact Andrea Carbonaro at [email protected]
For Research, Forensic or Paternity Use Only unless otherwise noted. Not for use in diagnostic procedures. STR kits described are for Forensic or Paternity Use Only. When used for purposed other than Human Identification the instruments and modules of the cited software are for Research Use Only. Not for use in diagnostic procedures.
The content provided herein may relate to products that have not been officially released and is subject to change without notice.
©2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. BSD is a trademark of Bizpac Party Ltd. FTA and EasiCollect are trademarks of Whatman Limited. NUCLEIC-CARD and FLOQSwabs are trademarks of Copan Flock Technologies Srl.
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