8/12/2019 Jmi Poster 2

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Determination of Heavy Metal Concentration in Both Field

Cultured and Tissue Cultured Kappaphycus alvarezii and

Gracilaria changiiusing ICP-OES

More than 60% of petroleum has been consumed since

1975 with world demand continuing to rise ~2% per year.

Biomass feedstock can replace more than 30% of

petroleum consumption [1]. Diacylglycerol acyltransferase

gene (DGAT; E.C. 2.3.1.20) from A. thaliana was

genetically engineered into tobacco (Nicotina tabacum) to

increase the oil yield for biofuel production.

Overview of DGAT

Figure 1. Triacylglycerol synthesis.

1. To compare oil yield between various crops.

2. To determine benefits and challenges for biofuel

production from engineered tobacco.

Oil Yield

Table 1. Comparison of oil yield between crops [2,3,4,5].

Oil yield in engineered tobacco can be maximized to 4.128

ton/acre. Hence, it increase the energy production per acre.

However, there are some benefits and challenges with theuse of tobacco for biofuel production.

1. Co-expression of DGAT with other genes involving in

oil synthesis and inhibition of negative feedback

system.

2. Maximization of oil yield by optimizing oil extraction

method on green tissue and reducing the lignin

content.

1. Ahmann et al. (2007). Bioengineering for Pollution Prevention throughDevelopment of Biobased Energy and Materials State of the ScienceReport (EPA/600/R-07/028).

2. Andrianov et al. (2009). Plant Biotechnol J, 8, 1-11.

3. Eshetu, B. (2000). http://ipp.boku.ac.at/pz/oilseeds/eshetu.html

4. OBrien, D. (2006).http://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdf

5. Razon, L.F. (2009). CAB Review: Perspectives in Agriculture, VeterinaryScience, Nutrition and Natural resources, 4(46), 1-14.

ABSTRACT

Various crops have always been seeked as biofuel, alternative to petroleum. Arabidopsis thalianadiacylglycerol acyltransferase (DGAT)

gene has been engineered into tobacco to increase oil yield both in seed and green tissue which showed higher oil yield compared to

soybean and rapeseed. The use of tobacco in biofuel production gives a lot of benefits but it faces challenges as well.

Figure 2. Comparison of oil yield between tobacco seed

and green tissue.

LITERATURE REVIEW

INTRODUCTION

CONCLUSION

MAIN LITERATURE CITED

AIMS

Low Lignin

Multi-production

Nonfood Crop

High Yield

Extraction Method

Cost

Benefits

Challenges

FUTURE RESEARCH

A full length cDNA of DGAT gene from A.thaliana was inserted into tobacco with a

strong ribulose-biphosphate carboxylase

small subunit promoter (rbcS Promoter) [2].

J.Y.Y. Chin, W.T.L. Yong, Suhaimi M. Yasir

http://ipp.boku.ac.at/pz/oilseeds/eshetu.htmlhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://www.pdc.us/pdf/bus_serv/target_industry/biodiesel-feedstocks-report.pdfhttp://ipp.boku.ac.at/pz/oilseeds/eshetu.html