kuliah 1 sejarah biotek
DESCRIPTION
sejarah biotekTRANSCRIPT
Topik Bentuk Tugas Mahasiswa Jumlah Pertemuan Dosen
I. Bioteknologi Pertanian meliputi Sejarah Singkat, Pengertian Dasar dan Ruang Lingkup
Ceramah dan diskusi
1 Agro
II. Teori Dasar Bioteknologi (Bahan Genetik, Replikasi DNA, Ekspresi Genetik dan DNA Rekombinan)
Ceramah dan diskusi
3 Agro
III. Teknik-Teknik Dasar bioteknologi (teknik pada tingkat protein dan DNA)
Ceramah dan diskusi
3 Agro (1)HPT (2)
IV. Evaluasi I 1 TimV. Bioteknologi
Konvensional dan Modern
Ceramah dan diskusi
2 HPT
VI. Bioteknologi Pemuliaan Tanaman
Diskusi kelompok
Telaah Jurnal
1 Agro+HPT (Tim Kelas)
VII. Bioteknologi Tanah
Diskusi kelompok
Telaah Jurnal
1 Agro+HPT(Tim Kelas)
VIII Bioteknologi Perlindungan Tanaman
Diskusi kelompok
Telaah Jurnal
1 Agro+HPT(Tim Kelas)
IX Regulasi Tanaman Produk rekayasa genetik
Ceramah dan diskusi
2 Agro (1)HPT (1)
X Evaluasi II 1 Tim
KONTRAK KULIAH BIOTEKNOLOGI
Acara Jadwal (Minggu ke)
Penanggung Jawab
I Isolasi/ekstraksi dan pengukuran Kandungan Protein
1 Prof. Tri Agus Siswoyo
II Pola Protein pada Tanaman ( Elektroforesis) 2 Prof. Tri Agus Siswoyo
III Isolasi/ekstrasi DNA (Plasmid) dan pengukuran Kandungan DNA/plasmid
3 Prof. Tri Agus Siswoyo
IV Elektrophoresis dan Visualisasi DNA 4 Prof. Tri Agus Siswoyo
V Amplifikasi DNA (PCR) 5 Prof. Tri Agus Siswoyo
VI Elektrophoresis dan Visualisasi PCR Produk 6 Prof. Tri Agus Siswoyo
VII Evaluasi 7 Prof. Tri Agus Siswoyo
INTRODUCTION TO PLANT BIOTECHNOLOGY
Biotechnology ?Biotechnology ?
The application of biological organisms, systems The application of biological organisms, systems or processes to manufacturing and service or processes to manufacturing and service industriesindustries
The integrated use of biochemistry, microbiology The integrated use of biochemistry, microbiology and engineering sciences in order to achieve and engineering sciences in order to achieve technological (industrial) application capabilities technological (industrial) application capabilities of microorganism, cultured tissue cells and part of microorganism, cultured tissue cells and part thereofthereof
A technology using biological phenomena by A technology using biological phenomena by copying and manufacturing various kinds of copying and manufacturing various kinds of useful substanceuseful substance
The application of scientific and engineering The application of scientific and engineering principles to the processing of materials by principles to the processing of materials by biological-agents to provide goods and servicesbiological-agents to provide goods and services
Biotechnology ?Biotechnology ?
The science of the production processes based on The science of the production processes based on the action of microorganisms and their active the action of microorganisms and their active components and production processes involving the components and production processes involving the use of cells and tissues from higher organisms. It use of cells and tissues from higher organisms. It was not included medical technology, agriculture was not included medical technology, agriculture and traditional crop breedingand traditional crop breeding
The use of living organism and their component in The use of living organism and their component in agriculture, food and other industrial processesagriculture, food and other industrial processes
The use of microbial, animal and plant cells or The use of microbial, animal and plant cells or enzymes to synthesize, breakdown and transform enzymes to synthesize, breakdown and transform materialsmaterials
The integration of natural sciences and organisms, The integration of natural sciences and organisms, cells, parts thereof and molecular analogues to cells, parts thereof and molecular analogues to product and servicesproduct and services
Stages of Biotechnology Stages of Biotechnology DevelopmentDevelopment
Ancient biotechnology Ancient biotechnology early history as related to food and shelter; early history as related to food and shelter; Includes domesticationIncludes domestication
Classical biotechnology Classical biotechnology built on ancient biotechnology; Fermentation built on ancient biotechnology; Fermentation promoted food production, and medicinepromoted food production, and medicine
Modern biotechnologyModern biotechnologymanipulates genetic information in organism; manipulates genetic information in organism; Genetic engineeringGenetic engineering
Traditional/old biotechnologyTraditional/old biotechnology
The conventional techniques that have been used to The conventional techniques that have been used to produce beer, wine, cheese, many other foodproduce beer, wine, cheese, many other food
New/modern biotechnologyNew/modern biotechnology
All methods of genetic modification by recombinant All methods of genetic modification by recombinant DNA and cell fusion techniques, together with the DNA and cell fusion techniques, together with the modern development of traditional biotechnological modern development of traditional biotechnological processprocess
Stages of Biotechnology Stages of Biotechnology DevelopmentDevelopment
Areas of BiotechnologyAreas of Biotechnology Organismic biotechnologyOrganismic biotechnology
uses intact organisms; Does not uses intact organisms; Does not alter genetic materialalter genetic material
Molecular biotechnologyMolecular biotechnologyalters genetic makeup to achieve alters genetic makeup to achieve specific goalsspecific goals• Transgenic organism- an organism Transgenic organism- an organism
with artificially altered genetic materialwith artificially altered genetic material
Biotechnology: Biotechnology: A collection of technologiesA collection of technologies
The Applications of The Applications of BiotechnologyBiotechnology
Medical BiotechnologyMedical Biotechnology DiagnosticsDiagnostics TherapeuticsTherapeutics VaccinesVaccines
Agricultural BiotechnologyAgricultural Biotechnology Plant agriculturePlant agriculture Animal agricultureAnimal agriculture Food processingFood processing
Environmental BiotechnologyEnvironmental Biotechnology Cleaning through bioremediationCleaning through bioremediation Preventing environmental problemsPreventing environmental problems Monitoring the environmentMonitoring the environment
Plant agriculturePlant agriculture
Crop production and protectionCrop production and protection• Genetically engineered (transgenic) cropsGenetically engineered (transgenic) crops• Using biological methods to protect cropsUsing biological methods to protect crops
• Exploiting cooperative relationships in natureExploiting cooperative relationships in nature
Nutritional value of cropsNutritional value of crops• Improving food quality and safetyImproving food quality and safety
• Healthier cooking oils by decreasing the concentration of Healthier cooking oils by decreasing the concentration of saturated fatty acids in vegetable oilssaturated fatty acids in vegetable oils
• Functional foodsFunctional foodsFoods containing significant levels of biologically active Foods containing significant levels of biologically active
components that impart health benefitscomponents that impart health benefits
Plant Biotechnology
TRADITIONAL PLANT BIOTECHNOLOGY
breeding
tissue culture
inter-specific hybridisation
mapping phenotypic/biochemical markers
Now use a molecular approach to manipulation: molecular markers & mapping
gene cloning
plant transformation
PLANT BIOTECHNOLOGY
a process to produce a genetically modified a process to produce a genetically modified plant by removing genetic information from plant by removing genetic information from
an organism, manipulating it in the an organism, manipulating it in the laboratory and then transferring it into a laboratory and then transferring it into a
plant to change certain of its characteristics plant to change certain of its characteristics ..
Exogenous genes (non-plant
genes)
Applications:
transgenic
pathogen-derived genes
bacterial genes
any other organism
Pathogen resistance
Herbicide resistance
bioreactors
Delivery systems
Endogenous genes (Plant
genes)
Gene discovery (functional genomics)
Applications:
markers
transgenic
Enzymes in biochemical pathway
Natural resistance genes
Marker assisted breeding
Plant improvement
Mapping
ESTs, libraries
Silencing, expressionMutants, arrays