29 2017

Laboratory of Applied Stress Microbiology

Manhattan from Hoboken

Serendipity !! Originality !!

Manhattan from Empire State Building

1982 1995 2006

Applied Molecular MicrobiologyCiti Field, New York

2 1

DC 5 MC 8 + 6 8 !!

!!

!!

吟醸香 AAT

(ATF1/2)�Isoamyl acetate�

TA�

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Keywords etc.

Keywords ROSNO

Rim15 TOR etc

Rim15

British Columbia etc.

pH etc.

!

•  • • • 

mRNA!

!

!

X!

response

tolerance

(adaptation)

L-

N-

N- -GSA

L-

L-

L- -γ- (GSA)

L-

L-

L- -5-

L-

N- -L-

Arg2

Arg8

Arg6

Arg5

Arg7

P5C/GSA

L-

Pro2

Pro3

L-Δ1- -5- (P5C) H2O

Arg3,1,4 Car1

Pro1 Pro1

N- -5-

Mpr1 Put1

Put2

CoA

Car2

NO Tah18

ROS ?

AZC

γ-

TOR

Appl. Microbiol. Biotechnol., 47, 405, 1997; 79, 273, 2008, 81, 211, 2008; FEMS Microbiol. Lett., 184, 103, 2000; Appl. Environ. Microbiol., 69, 212, 2003; 69, 6527, 2003; 71, 8656, 2005; 73, 4011, 2007; 74, 5845, 2008; J. Biosci. Bioeng., 94, 2002; 100, 538, 2005; 103, 277, 2007; 116, 576, 2013; Biosci. Biotech. Biochem., 73, 2131, 2009; 76, 454, 2012; Int. J. Food Microbiol., 152, 40, 2012; 238, 233, 2016; J. Gen. Appl. Microbiol., 62, 132, 2016; Yeast, 33, 353, 2016; FEBS Lett., 590, 2906, 2016; Microbial Cell, 3, 522, 2016.

Arg

Mpr1

Mpr1

S. cerevisiae Σ1278b：AZC 耐性株�

AZC N-

ROS

Pro/Glu Arg NO

J. Bacteriol., 182, 4249, 2000; J. Biol. Chem., 276, 41998, 2001; Yeast, 19, 1437, 2002; 26, 587, 2009; J. Biochem., 133, 67, 2003; 138, 391, 2005; Proc. Natl. Acad. Sci. USA, 101, 12616, 2004; 110, 11821, 2013; Appl. Microbiol. Biotechnol., 75, 1343, 2007; 97, 247, 2013; FEMS Yeast Res., 8, 607, 2008; Biotechnol. Bioeng., 103, 341, 2009; Int. J. Food Microbiol., 138, 181, 2010; J. Biosci. Bioeng., 114, 160, 2012; J. Biochem., 159, 271, 2016 etc.

CO2HNH

L-����-�� �� (AZC)

NH

CO2H

HO

��-4-����-L-���� (CHOP)

NO

!!

mpr1Arg NO

NO Tah18-Dre2 NO

NO Mac1

NO etc.

NO Mpr1 Arg Tah18 NO

NO Mac1

Mac1�

Nucleus�

NO�

CTR1�

Arg�

Ctr1�Cu+�

Ctr1�

Fre1�

Cu2+�

ROS�

Tah18� Sod1�

Sod1�Sod1�S-����� ?�

ストレス耐性 !! 高温�

FEMS Yeast Res., 10, 687, 2010; Microb. Cell Fact., 11:40 doi:10.1186/1475-2859-11-40, 2012: Proc. Natl. Acad. Sci. USA, 110, 11821, 2013; Biochem. Biophys. Res. Commun., 430, 137, 2013 PLoS One, 9, e113788, 2014; Nitric Oxide-Biol. Chem., 52, 29, 2016; 57, 85, 2016; Appl. Microbiol. Biotechnol., 100, 9483, 2016.

Rsp5

Rsp5

Rsp5

Rsp5

Rsp5

Rsp5 AZC

Proc. Natl. Acad. Sci. USA, 100, 11505, 2003; FEBS Lett., 580, 3433, 2006; Biosci. Biotech. Biochem., 70, 2762, 2006; 73, 2268, 2009; FEMS Microbiol. Lett., 277, 70, 2007; Genes Cells, 13, 105, 2008; FEMS Yeast Res., 9, 73, 2009; 14, 567, 2014; FEBS J., 276, 5287, 2009 ; J. Brew. Distill., 3, 1, 2012, Genes Cells, 18, 459, 2013; Eukaryot. Cell, 13, 1191, 2014; J. Biochem., 157, 251, 2015 etc.

rim155055insA

Rim15 !!

Rim15

Greatwall

Rim15Appl. Environ. Microbiol., 77, 934, 2011; 78, 385, 2012; 78, 4008, 2012; J. Biosci. Bioeng., 112, 44, 2011; 112, 54, 2011; 112, 577, 2011; 116, 591, 2013; 123, 183, 2017; Biosci. Biotech. Biochem., 77, 2255, 2013; Appl. Environ. Microbiol., 82, 340, 2016.

!!

FEMS Yeast Res., 16, fow049, 2016; J. Cell Sci. 128, 1762, 2015; Biosci. Biotechnol. Biochem., 78, 1389, 2014; Cell Struct. Funct., 38, 135, 2013; Biosci. Biotechnol. Biochem., 77, 1337, 2013; Genes Cells 18, 288, 2013; Mol. Biol. Cell, 22, 3520, 2011; Curr. Opin. Cell Biol., 23, 135, 2011; J. Cell Biol., 179, 75, 2007; J. Cell Biol., 167, 445, 2004; Mol. Biol. Cell, 14, 2559, 2003.

ER !!

ER ?

��"%)"

��"%)"#* +Ire1

������� ,"%)"!�&(-

��

������� ,�����$*'�����-

"%)"�����

��"%)"�

小胞体ストレスに伴う、Ire1の集合

ストレス無し������������������� ストレス時�(DTT$10mM$30min) �

Ire1-GFPの細胞内分布

Ire1

小胞体 サイトゾル�

�����

ER

etc.

Ire1 ER

? !!

ER

NONO

! Rsp5

→ Rsp5 α-

Univ. Medical Center Goettingen Univ. Bayreuth (Geremany)

!!

!! !!

!!

The 10th ANNIVERSARY

!!

, , , , , , , , , , , , , , , J&J,

, , , , , ,

!!

!!

5 International Meeting on Yeast Apoptosis (Italy), NO 6 International Specialized Symposium on Yeast (Ireland) 7 International Union of Microbiological Societies (Singapore)

9 , 12 , 3 etc.

!!

!!

5 M1 Beer & BBQ

12 Bowling → Eating/Drinking → Singing

3

!!