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Hndaw Pblshn CopoatonEvdnc-Basd Complmntay and Altnatv MdcnVolm 2013, Atcl ID 432764, 6 pashttp://dx.do.o/10.1155/2013/432764

Research ArticleLaser Acupuncture: Two Acupoints (Baihui, Neiguan)and Two Modalities of Laser (658 nm, 405 nm)Induce Different Effects in Neurovegetative Parameters

Gerhard Litscher,1,2 Lu Wang,1 Xiaoyu Wang,1,2 and Ingrid Gaischek1

1 Stronach Research Unit for Complementary and Integrative Laser Medicine,Research Unit of Biomedical Engineering in Anesthesia and Intensive Care Medicine,

TCM Research Center Graz, Medical University of Graz, Auenbruggerplatz 29,8036 Graz, Austria

2 Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences,Beijing 100700, China

Cospondnc shold b addssd to Ghad Ltsch; [email protected]

Rcvd 19 Apl 2013; Accptd 17 May 2013

Acadmc Edto: W H

Copyht 2013 Ghad Ltsch t al. Ts s an opn accss atcl dstbtd nd th Catv Commons AttbtonLcns, whch pmts nstctd s, dstbton, and podcton n any mdm, povdd th onal wok s poplyctd.

T a only wscntfc pblcatons daln wth thbascnvstaton oth ctso only ono twoacponts ocompanon snl pont wth anoth snl pont, sn dnt stmlaton mthods n th sam psons. T am o ths ndl-contolld, andomzd cossov stdy was to nvstat th novtatv paamts hat at (HR) and hat at vaablty(HRV) sntwo dnt acponts, Bah (GV20)and Nan (PC6), n spaat sssons. W nvstatd 11 halthy volnts(3 m, 8 ) wth a man a SD o22.9 2.8 yas. T two acponts w stmlatd o 10 mnts ach wth manal ndlacpnct, d las acpnct (658 nm), and volt las acpnct (405 nm), n andomzd od. Ndl and d lasstmlaton o th Bah acpont dcasd HR snfcantly. Only volt las stmlaton at th Nan acpont ndcd asnfcant ncas o total HRV. Fth stds sn oth novtatv paamts and mo volnts a ncssay toconfm th plmnay slts.

1. Introduction

T tm las s on connctd wth pcson, t,

and nnovaton. Only vy w oth aas hav shown scha apd and nomos dvlopmnt ov th last yas asmdcal las applcatons. Hh-tch las acpnct [1],ntavnos las blood adaton [2], and nonnvasv andnvasv las ndl stmlaton [13] a only som o tht-ontd las thapy optons n mdcn.

O sach op was th fst to nvstat voltlas acpnct (405 nm). Impotant contbtons on thstopc w pblshd btwn 2010 and 2012 [48]. T mostmpotant dnc btwn volt and, o xampl, dlas (658 nm) s th pntaton dpth (405nm: 1-2 mm;658 nm: 3-4 cm) on th on hand and th act that th ntny o th volt las s absobd alady at th skn

sac on th oth hand. In pactcal tms, ths mans thatth d las cannot b lt, whas th volt las can, thsndcn a ston dq snsaton [9].

T am o ths ndl-contolld, andomzd cossovstdy was to nvstat novtatv paamts lk hatat (HR) and hat at vaablty (HRV) n two dntsssons sn two dnt acponts n halthy volnts.

2. Materials and Methods

2.1. Stimulation Methods

2.1.1. Manual Needle Acupuncture. Ndl acpnct waspomd wth snl-s stl ndls (0.30 30mm,Han Q, Szho, Chna). A dsnctn th skn at th


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2 Evdnc-Basd Complmntay and Altnatv Mdcn

Figure 1: Las ndl systm at th Mdcal Unvsty o Gaz.

chosn acpont(s) (s blow), th ndl was nstd andstmlatd clockws and contclockws o 15 scondsach, wth two otatons p scond, sltn n 30 otatons[10]. A tn mnts, th ndl was movd.

2.1.2. Red Laser. T d las ndl adaton (658 nm) wascopld nto optcal fbs, and th las ndls waand at dstal nds o th optcal fbs o a Lasndl-toch systm (Lasndl GmbH, Bln, Gmany; sF 1). Otpt pow o ach las ndl was 40 mW. T

fb co damt was abot 500 m. T tm o adatonwas 10 mnts, sltn n an ny dnsty o abot

20 J/cm2 p acpont. A contnos wav (cw) mod wasappld. T ndls w placd vtcally on th skn snspcal applcatos, tn panlss and nonpcptblstmlaton at th acpont. T mthod s dscbd n dtaln pvos pblcatons [11].

2.1.3. Violet Laser. Nonnvasv volt optcal las ndls(wavlnth: 405 nm, otpt pow 110 mW, las ndl spotdamt 500 m, daton: 10 mn, cw-mod) w also fxdonto th skn, bt not nstd. T sam las ndl systmdscbd abov was sd. Optcal ny dnsty was vy

hh (an: kJ/cm2). Mo dtals concnn th tchncalpaamts o las ndl acpnct can also b ond ncnt pblcatons [12].

2.2. Neurovegetative Monitoring. T two novtatvpaamts w codd sn an HRV Mdlo AR12(Hntlh Halthca, Cad, UK; and Lpamd GmbH,Gaz, Asta) systm. Ts systm s dsnd o a monto-n pod p to 24 hos. T sampln at o th cods 4096 Hz, so that R wavs can b dtctd xtmlyaccatly. T Skntact Pm F-55 ECG lctods(Lonhad Lan GmbH, Innsbck, Asta) w fxd on

th chst. All aw data a stod dtally on spcal mmoycads.

HRV s masd as th pcnta chan n sqntalchamb complxs (RR ntvals) n th ECG. HRV can bqantfd ov tm sn staton o pcnta chansn RR ntvals n th tm doman as wll as th chans

n th qncy an by analyss o lctocadoaphcpow spcta. Paamts a commndd by th taskoc o th Eopan Socty o Cadoloy and th NothAmcan Socty o Pacn and Elctophysoloy [13].Calclaton o ECG pow spcta s thoht to povd anndstandn o th cts o sympathtc and paasym-pathtc systms on HRV and s sd also n acpnctsach [1323]. Ealy wok pontd ot a w bands nth spctm o HRV that cold b ntptd as makso physolocal lvanc. Assocatd mchansms a th-molaton whch can b ond n th vy low qncyband, blood pss and spatoy cts [13]. o th bsto o knowld, th a no scntfc slts concnnlas acpnct and lon-lastn thmolatoy ctswhch mht b ctd n HRV.

2.3. Volunteers. T nvstatons w pomd n lvnhalthy volnts (M/F, 3/8) wth a man a SD o22.9 2.8 yas. Body hht was 172.9 7.4 cm and body wht66.0 10.4 k. Non o th sbjcts was nd th nnco any mdcaton. T staton o th non-nvasvpaamts was appovd by th local thcs commtt andn accodanc wth th Dclaaton o Hlsnk o th WoldMdcal Assocaton. All psons povdd wttn nomdconsnt.

2.4. Acupuncture Points. T ollown acpnct pontsw sd n o stdy: Bah (GV20) and Nan (PC6)(s Fs 2(a) and 2(b)). Bah s on o th mostmpotant acponts o th D mdan (ovnn vssl)and commonly sd n noloy and psychaty [24]. It slocatd on th contnaton o th ln connctn th lowstand hhst ponts o th a, on th mdan ln o thhad, 7 cn abov th posto haln, and 5 cn bhndth anto haln [25]. Nan s locatd on th palmasd o th oam, on th ln connctn Qz (PC3) andDaln (PC7), 2 cn poxmal to th tansvs cas oth wst, and btwn th tndons o m. palmas lonsand m. xo cap adals [26]. Ts pont was stmlatd

blatally (s F 2(b)). Its man ndcatons a cadacpan, palptaton, oppssd ln on th chst, vomtn,plpsy, mana, and bl dsass.

2.5. Measurement Procedure. T pocd was dvdd ntoth pats: volt las, d las, o ndl acpnct,whch w pomd n th sam sbjcts n a andomzdod. Btwnth dnt stmlaton modalts, thwasa bak o at last 10 mn. Each o th acponts dscbdabov was tstd n all sbjcts n spaat sssons on twodnt days (coss-ov), also n andomzd od. Tpatcpants w lyn comotably on a bd dn th ntnvstaton.


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(a) (b)

Figure 2: Acpnct ponts Bah (GV20; (a)) and Nan (PC6; (b)).

b d1 d2 a

5 min

10 min

stimulation

Figure 3: Rcodn pofl. Manal ndl stmlaton, d las(658 nm), and volt las (405nm), n andomzd od. (b)bo stmlaton; (d1, d2), two phass dn stmlaton; (a) astmlaton.

T masmnt pocd, and th 5-mnt smnts(altoth 20 mn) a shown n F 3.

2.6. Statistical Analysis. Data w analyzd wth on-waypatd mass analyss o vaanc (ANOVA; SmaPlot12.0, Systat Sowa, Chcao, IL, USA), and th ky tstwas sd o post hoc analyss. T lvl o snfcanc wasdfnd as < 0.05.

3. Results

F 4 shows HR vals om th masmnts. Dnand a stmlaton o th Bah acpont, HR dcasd;howv, th ct was snfcant only dn and andl and d las stmlaton. Stmlaton o Nan ddnot ndc any snfcant chans n HR.

T vals o total HRV o all 11 halthy volntsa smmazd n F 5. Nth ndl no d lasno volt las stmlaton at th Bah acpont ndcdsnfcant chans n HRV, althoh HR dcasd dnand a all th stmlaton modalts (c. F 4).Howv, volt las stmlaton o th Nan acpontsshowd a snfcant ncas dn th scond hal o thstmlaton phas (d2) n compason to th basln vals

(b) and also to th fst hal o th stmlaton phas (d1). Its ntstn that ndl and d las stmlaton dd notndc smla cts.

4. Discussion

Acpnct, an ontal mdcn tchnq that can btacd back at last 2,500 yas, s ann poplaty as analtnatv and complmntay ntvnton n th Wstnwold. Accodn to tadtonal Chns mdcn (CM),acponts a dstbtd alon mdans bnath th bodyssac [27]. In CM, th snl acpnct ponts wllonhav dnt cts, althoh thy a sd n th tatmnto th sam dsas. Whn nvstatn th ctvnsso acpnct on ctan dsass, acpont combnatonso schms a sally sd. T a only w scntfcpblcatons daln wth th basc nvstaton o thcts o only on o two acponts. T a vn wstds compan on snl pont wth anoth snl pont,sn dnt stmlaton mthods n th sam psons, aspomd n ths stdy.

Bah (GV20) s on o th most mpotant acpontso th nt mdan acpont systm. It was potd thatlctoacpnct stmlaton at GV20 ncasd th c-bal pson n th cbal cotx whch was sppssd nndothlal ntc oxd synthas knockot mc [28] and also

ncasd cbal blood ow n a modl o schmc bannjy n ats [29]. T w also pots o acpnctstmlaton o GV20 wokn wll n lown blood pssnot only n hyptnson patnts [30] and halthy sbjcts[31] bt also n spontanosly hyptnsv ats [32]. Smlacts may b lvant o dcasn abnomally lvatdltamat and actylcholn lvls n th lsond sd oth statm [33]. Anoth aspct o ths stdy on th cto GV20 was that th pont was ascbd th ablty tocalm and stablz motons [33]. In th clncal pocdo patnts wth slp dstbancs, Bah s th mostcommonly sd acpnct pont [34, 35]. Acpnct onGV20 mtatd th anxty symptoms o womn ndon


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b d1 d2 a b d1 d2 a b d1 d2 a


P 0.016 P 0.002

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HRmean

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Figure 4: Box plot psntaton o HR slts. Not th snfcantdcas o HR (ndl and d las acpnct). T lns n th

boxs psnt th mdan; th nds o th boxs, th 25th and75th pcntl; th o bas, th 10th and 90th pcntl; and thdots, th otls. (b) Bo stmlaton; (d1, d2), two phass dnstmlaton; (a) a stmlaton.

n vto tlzaton [36] andpatntsdn dntal tatmnt[37]. GV20 was ond to hav th ablty o vasodlataton anddcton o sympathtc actvty n th stss spons [2832]. In th psnt stdy, w ond GV20 to hav th cto dcasn HR, whch s n accodanc wth th pvoslymntond cts.

Nan (PC6) s also a classc acpnct pont n

CM. It s consdd to b ctv whn tatn ca-dovascla dsods. Accmlatn scntfc vdnc hascntly shown that PC6 cold modlat cadovasclanctons, possbly thoh actvaton o th ostal vnto-latal mdlla (RVLM) aa [38, 39]. It was also potdthat stmlaton o PC6 dcasd th xtnt o myocadalschma by mans o dcn th myocadal oxyndmand n anmals, and t dcd sympathoxctatoy ca-dovascla x sponss, patly thoh an ct on thRVLM [40, 41]. Anoth stdy sstd that PC6 and S36both actd cadac actvts n halthy volnts [42].In pots om patnts sn om ccadan hythmdsods, las acpnct stmlaton appld on PC6

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/Tukeytest


P = 0.009

P = 0.007



103

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HRVtotal(a.u.)

HRVtotal(a.u.)

Figure 5: Chans n total HRV. A snfcant ncas occdonly dn volt las stmlaton o th Nan acpont. Fo

th xplanatons, s F 4.

ncasd vaal actvty and sppsson o cadac sympa-thtc nvs [43]. Howv, anoth stdy sstd thatHRV was not nncd by las ndl acpnct at thNan pont (PC6) [44]. Ts s smla to th obsvatonsn o xpmnt. Fth stds concnn possbl lon-tm cts o dnt knds o lass on novtatvpaamts a dsabl.

5. ConclusionsT ollown conclsons can b dawn om th slts oths stdy.

() Ndl and d las stmlaton o th Bah ac-pont dcas hat at n hman sbjcts snf-cantly.

() Only volt las stmlaton at th Nan acpontndcs a snfcant ncas o total hat at vaa-blty. Ts s vn mo ntstn bcas o th actthat at th sam tm HR dd not chan snfcantly.


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Conflict of Interests

T athos dcla to hav no conct o ntsts.

Acknowledgments

T scntfc nvstatons w sppotd by th StonachMdcal Gop, th Gman Acadmy o Acpnct(DAA), th Dpatmnt o Scnc o th Cty o Gaz,Easa-Pacfc Unnt, and th Astan Mnsts o Sc-nc and Rsach and o Halth. T masmnts wpomd wthn th sach aas o Sstanabl HalthRsach and Noscnc at th Mdcal Unvsty oGaz.

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