laser-spectroscopic information on agricultural products as future resources for field server...

17
Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito*, T. Koga*, T. Matsubara*, Y. Maruyama*, F. Kobayashi*, A. Nomura*, H. Ishizawa** and K. Komatsu*** *Shinshu University (Engineering), Nagano, Japan **Shinshu University (Textile Science & Technology), Ueda, Japan ***Nagano Vegetable and Ornamental Crops 17th APAN Meetings, University of Hawaii 2004.1.28-30

Upload: madeline-carroll

Post on 27-Mar-2015

214 views

Category:

Documents


1 download

TRANSCRIPT

Page 1: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Laser-spectroscopic Information on Agricultural Products as Future

Resources for Field server

Yasunori Saito*, T. Koga*, T. Matsubara*, Y. Maruyama*, F. Kobayashi*,

A. Nomura*, H. Ishizawa** and K. Komatsu***

*Shinshu University (Engineering), Nagano, Japan**Shinshu University (Textile Science & Technology), Ueda, Japan***Nagano Vegetable and Ornamental Crops Experiment Station,

Nagano, Japan

17th APAN Meetings, University of Hawaii2004.1.28-30

Page 2: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

MotivationFuture field server (next generation) will be requiredmore direct information on health and living status of agricultural products.

PurposeApplication of laser spectroscopy as a new method to collect the direct status information of agricultural productsDiscussion on its possibility and usefulness

Field Server

TemperatureHumiditySun LightCO2Water ContentImage....

Environment Information

+

Direct Status Information

PhysiologicalBiologicalBiophysicalPhotobiologicalEcophysiological…

FurtureField Server

Page 3: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

MethodologyLaser-induced Fluorescence Spectroscopy (LIFS)

LIFS for Agriculture Project

Fluorescence EmissionLaser

SampleMolecular

Energy Diagram

Normal

Abnormal

Spectrum

Vegetation Fluorescence as an Index for Photosynthesis, Stress, Disease, Vitality, etc.No-use Chemical SolutionNon Destructive MethodRemote Sensor toward OPTICUL FARMING

Page 4: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Examples

1) Lettuce : Optimal Harvest Time Decision2) Coffee : Leaves Vitality (?) Distribution Map3) Tomato : Seedling’s Remote LIF Imaging

Page 5: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

   1) Lettuce : Optimal Harvest Time Decision

PC

Spectrometor& CCD detector

Nd:YAGLaser

Laser TransmissionFiber

Fluorescence DetectionFiber

0

0.5

1

1.5

2

300 400 500 600 700 800Wavelength (nm)

Rela

tive I

nte

nsit

y

LIF Spectra of Lettuce Leaf

Mobile LIF Monitoring System

ChlorophyllFerulic Acid& Others

Page 6: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Ch

loro

gen

ic a

cid

 C

on

cen

trati

on

g/g

)

0

20

40

60

80

100

0 10 20 30 40 50 60

Days after Planting

▲ ‘98 ‘99

● ‘ 97▲ ‘ 98  ■  ‘ 99   

Outer Leaves

0

0.5

1

1.5

Rela

tive I

nte

nsit

y

‘99‘98‘97Harvest time

■■

Page 7: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

2 ) Coffee Tree Leaves : Vitality (?) Distribution Map

Image IntensifiedCooled CCD

Camera

CW VioletDiode Laser

Liquid CrystalTunable

Filter

PC

Coffee Tree LIF Imaging System

Controll Units

Page 8: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

460nm 530nm 685nm 740nm

Ferulic acid derivativesPhenylpropanoidsNAD(P)H

Chlorophyll

0 5

Activated?

Relative Value to 660nm Fluorescence Image Intensity

Page 9: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

3) Tomato : Seedling Remote LIF Imaging

1.5m

0.3m

CameraController

Gated ICCDCamera

Liquid Crystal Tunable Filter

Tomato Seedling

Fluorescence

Nd:YAG Laser

PC

15m

Page 10: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

600 700 800

Wavelength (nm)

F660/F620

F685/F620

F720/F620

F740/F620

Remote Spectral Imaging of LIF

Page 11: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Conclusion Proposal : LIF information for a future field server System development and discussion of the usefulness 1) Lettuce : Optimal harvest time decision 2) Coffee tree leaves : Vitality distribution map 3) Tomato : Remote LIF imaging

 

Needs direct comparison to physiological data (evidence) More experimental discussion required

Page 12: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Conclusion Proposal : LIF information for a future field server System development and discussion of the usefulness 1) Lettuce : Optimal harvest time decision 2) Coffee tree leaves : Vitality distribution map 3) Tomato : Remote LIF imaging

 

Needs direct comparison to physiological data (evidence) More experimental discussion required

Field Server

Plan

Page 13: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

Thank you very much !!

Mahalo Nui Loa !!

Page 14: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,
Page 15: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

1) LIF スペクトル情報取得

   ex 2 ) 形態付属情報の取得        実験方法        ハウス管理栽培トマト葉         移動型 LIF スペクトル計測装置

Page 16: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

0

12

3

45

67

89

- A6471shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度 1系列#

7 - A6411shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度 1系列

#1

- A6491shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度 1系列#

9

- A6441shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度 1系列#

4 - A6421shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度 1系列#

2

A64-0

-50

0

50

100

150

200

250

300

200 300 400 500 600 700 800 900

波長(nm)

強 1系列#0

Page 17: Laser-spectroscopic Information on Agricultural Products as Future Resources for Field server Yasunori Saito *, T. Koga*, T. Matsubara*, Y. Maruyama*,

- A6431shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度

1系列

- A6451shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度

1系列

- A6481shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度

1系列

- A6461shot

-50

0

50

100

150

200

250

300

350

200 300 400 500 600 700 800 900

波長(nm)

強度

1系列

#3

#8

#5

#6