lltech breast atlas 2012
DESCRIPTION
This document present the result of the imaging capabilities for ex vivo breast tissue.TRANSCRIPT
1 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
LLTech Light-‐CT Scanner Breast images 2012
2 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
• Op8cal in-‐depth biopsies of gross 8ssue within minutes
• 1 µm 2D and 3D histopathological resolu8on
• Easy explora8on, acquisi8on and rendering in DICOM format
• Safe, non-‐invasive and non-‐destruc8ve process
Light-‐CT™ key benefits
Fast and non-‐invasive 3D in-‐depth structural and cellular imaging
3 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Based on Full-‐Field Op8cal Coherence Tomography (FFOCT)
Combines microscope resolu8on with interferometry
High resolu8on in-‐depth C scans
Commercial device specs:
• Excellent resolu8on: 1,5µm transverse, 1µm axial
• 70Hz max. tomographic frame rate – 0.8 mm x 0.8 mm
• Penetra8on depth 200µm – 1mm depending on 8ssue scaZering
• 25 mm diameter sample size
• Small footprint: Scanner and light source fit on 70 cm x 35 cm
Light Computed Tomography technology
4 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Op8cal acquisi8on unit, moving ver8cally • User friendly
acquisi8on so\ware
• DICOM 2D and 3D Viewer
Movable tray with sample holder
X,Y moving stage
Joys8ck for easy control of X,Y, Z movements
White Light Source
Integrated wide field camera to take sample picture before
imaging
Light-‐CT™ Scanner for ex-‐vivo cellular imaging
5 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
BREAST IMAGING
6 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Grainy aspect of normal fibrous 8ssue
Duct with calcifica8on
Lobule Adipocytes Vessel
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
Healthy breast <ssue
7 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com © LLTech 2012 Figure 3 : Diagnosis decision tree for FF-‐OCT images on human breast tissue
Breast tissue reading tree
8 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Breast Histology DCIS – Ductal Carcinoma in situ
Breast., Ductal Carinoma H&E vs LightCT on Fresh Tissue Hopital Tenon, France, August 2010
Lobules
Fat cells
1 cm
9 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Breast Histology DCIS – Ductal Carcinoma in situ
Lobules
Duct with necrosiss
10 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Breast Histology Invasive adenocarcinoma - Nodular tumor
Tumorous 8ssue: highly scaZering thin trabeculae aspect of fibers
Healthy 8ssue: grainy medium scaZering fibers
11 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Breast Milk duct with thick wall
1 mm
3 mm
1
Milk Duct
2
Blood vessels
Traditional Histology
1 mm
Connective tissue
Fat cells
12 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Breast Histology Identification of the milk duct structures
Milk Duct End
Fat Cells
13 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Healthy breast <ssue
Lobule
Galactophorous duct cut longitudinally
Honeycomb configura8on of adipocytes
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
14 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Collec8ng duct with secre8on in the lumen
Collec8ng duct in the retroareolar sec8on
Nipple -‐ Cross sec<on
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
15 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Invasive adenocarcinoma with in Situ Component
Enlarged ducts filled with cells prolifera8on or necrosis
Highly scaZering thin trabeculae aspect of fibrous 8ssue
surrounding grey cellular zones
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
16 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Elongated compressed ductules with slit-‐like lumen
Well delimited nodule with lobulated appearence
Fibroadenoma
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
17 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Fibroadenoma
Enlarged ductules characteris8c of the lesion
© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France
18 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
The Light-CT scanner allows fast tissue Processing and Pathology Examination. It completes the tools available for pathologists
When Chemical Fixation is needed
(Mostly Diagnostic)
When Frozen Section is needed
(Mostly Intra-operative)
Biopsy 3D
Digital Image
2D Digital Image
Slide
15-12 mins Artefacts
Destructive
12-24 hours Expensive Destructive
5-8 minutes Non-Destructive
Tissue Scanner
19 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
The Light-CT could help reducing the number of re excision in breast cancer surgery whilst preserving the current habits of pathologists
Per surgery
LLTech’s analysis
Histology And/or
Immuno histochemistry
Further treatment
Post surgery
Surgical margin analysis Metastasis analysis (> 2mm) et
micro metastaisi : PN1mi > 0.2 – 2 mm <
Surgery OK OK
Per surgery Post Surgery
Cur
rent
tech
niqu
es
Up
to 4
0% o
f dou
ble
proc
edur
es
LLTe
ch L
ight
-CT
Sig
nific
ativ
e de
crea
se o
f do
uble
pro
cedu
res
Visual analysis, cryostat
Further treatment Surgery
OK
Cancerous cancerous
OK
Poor identification of metastasis
Up to 40% of double surgical procedures
Several days analysis Few minutes analysis
Surgeon Pathologist Pathologist
Decrease of double surgical procedures Cancerous Cancerous
No surgical margins analysis
Histology And/or
Immuno histochemistry
Node
Margin
Several days analysis Few minutes analysis
Surgeon Pathologist Pathologist
20 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com
Contact Details
USA
LLTech Inc. 103 Carnegie Center Drive
Suite 300 Princeton, NJ 08540
USA
www.lltechimaging.com [email protected]
Phone :+ 1 609 955 3506
LLTech SAS 6, place de la Madeleine
75008 Paris - France
www.lltechimaging.com
Phone :+33 9 72 16 33 40
Europe