master- and bachelor thesis topics 2018-2019 master

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1 Master- and Bachelor Thesis Topics 2018-2019 Master-/bachelor-oppgaver Plant protection Plantevernfag Her er mange forslag til spennende oppgaver innen plantevernfag, d.v.s. ugras/herbologi, plantepatologi, landbruksentomologi og pesticidkjemi. Dersom du vil finne ut mer om oppgavene ta kontakt med veilederen(e) som er oppført, med kopi til faggruppelederen i Plantevern og Matplanter [email protected]. Du kan også komme med eget forslag til oppgaver. Below you will find many interesting thesis topics in plant protection, including weed science, plant pathology, agricultural entomology and pesticide science. If you want to know more about a topic, contact the supervisor listed for the topic, and copy to the head of section [email protected]. You can also suggest a topic that is not listed here.

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Page 1: Master- and Bachelor Thesis Topics 2018-2019 Master

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Master- and Bachelor Thesis Topics 2018-2019 Master-/bachelor-oppgaver

Plant protection

Plantevernfag

Her er mange forslag til spennende oppgaver innen plantevernfag, d.v.s. ugras/herbologi,

plantepatologi, landbruksentomologi og pesticidkjemi. Dersom du vil finne ut mer om

oppgavene ta kontakt med veilederen(e) som er oppført, med kopi til faggruppelederen i

Plantevern og Matplanter [email protected]. Du kan også komme med eget forslag til

oppgaver.

Below you will find many interesting thesis topics in plant protection, including weed science,

plant pathology, agricultural entomology and pesticide science. If you want to know more

about a topic, contact the supervisor listed for the topic, and copy to the head of section

[email protected]. You can also suggest a topic that is not listed here.

Page 2: Master- and Bachelor Thesis Topics 2018-2019 Master

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Agricultural entomology

Landbruksentomologi

Page 3: Master- and Bachelor Thesis Topics 2018-2019 Master

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28 Effect of UV-B used against plant diseases on biological pest management in

greenhouses and plastic tunnels (UV-Bio)

Supervisor: Nina Svae Johansen, NIBIO/NMBU. [email protected]

Background: Biological control of insect and mite pests is the most important control

method in greenhouse cucumber, tomato, strawberries, herbs and berry crops today, and is

also used in berries grown in plastic tunnels. Powdery mildew is a great problem in these

crops, and is today mainly controlled by fungicides. Recent research has, however, shown that

this plant disease can be controlled by treating the plants with very low dosages with

ultraviolet B wavelengths (UVB) during the night. A robot for automatic UVB-treatment is

currently under development for use in commercial greenhouses. The growers want to use

less fungicides, and therefore have great interest in the robot, but they need to be sure that the

UVB-treatment is safe for the biocontrol agents (= natural enemies of the pests used in

biological control) and does not interfere with biological control.

The aim of this project is to be able to combine biological pest control with UVB-treatment

against powdery mildew in edible crops grown in greenhouses and plastic tunnels (cucumber,

tomato, strawberries and herbs).

Model organisms:

Pests: Two-spotted spider mite and aphids

Biocontrol agents: Predatory mites, predatory bugs and aphid parasitoids.

Fig. 2 Adult and egg of two-

spotted spider mite (Photo:

K. Westrum, NIBIO)

Fig. 3 The predatory bug

Macrolophus is used

against spider mites and

whiteflies (Photo: E.

Fløistad, NIBIO)

Fig. 4 Parasitoids and

parasitized aphids (Photo: E.

Fløistad, NIBIO).

The research questions that we will look into are:

UVB is harmful to insects and mites if they are exposed in sufficiently high dosages. But

how and to what degree will the low UVB dose used to control powdery mildew affect the

different pests and biocontrol agents?

Page 4: Master- and Bachelor Thesis Topics 2018-2019 Master

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Will the mobile stages of the different pests and biocontrol agents try to avoid the UVB-

irradiation? Will the avoidance response be different in the different species?

Many herbivore pests live on the underside of the leaves or otherwise hidden in the plant

canopy. Green light is attractive to many pests and biocontrol agents. Can green light be

used to lure the pests to the UVB-irradiated parts of the plants? How will the green light

affect the biocontrol agents?

Pests and biocontrol agents may respond differently on the UVB-treatment. Will this

influence the balance between pests and biocontrol agent population development? Will

this affect the success of the biological pest control in the greenhouse and plastic tunnels?

If UVB has negative impact on biological control: How can the use of biocontrol agents or

the use of the UVB-equipment be adjusted to compensate for this?

Project period: 01.03.2016 – 28.02.2019.

Time for doing experiments: All year round, depending on type of study

Type of studies: Basic studies of biology and behaviour of pests and biocontrol agents in

laboratory and research greenhouses and/or studies of population development and biological

control in commercial greenhouses (cucumber, tomato and herbs, plastic tunnels with

strawberries).

Page 5: Master- and Bachelor Thesis Topics 2018-2019 Master

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29 Optimal strategy for the use of pesticides in Norwegian berry production

(OptiBær). Work package 2: Reduced risk of pesticide resistance development in

two- spotted spider mite

Supervisor: Nina Svae Johansen, NIBIO/NMBU. [email protected]

Background: Two-spotted spider mites have a high risk of developing resistance to

acaricides (= pesticides used against mites). The lack of effective alternative control methods

and the few acaricides with different modes of action available for use against mite pests in

Norwegian berry crops further increase the resistance risk. Globally, the two-spotted spider

mite has developed resistance to 94 different active ingredients (479 cases) in total, amongst

them are four out of the five active ingredients that are registered for mite control in berry

crops in Norway. Acaricide resistance in two-spotted spider mites is very little studied in

Norway. However, indications of resistance to two active ingredients was found around the

year 2000, and the growers strongly suspect resistance in e.g. strawberries.

The aim of work-package 2 in this project is to develop a strategy for reducing the risk of

pesticide resistance development in spider mites in Norwegian strawberry and raspberry

production.

Two-spotted spider mite

and mite damage in

raspberry. Photo: E.

Fløistad (left.) og N.

Trandem (right),

NIBIO.

The research questions that we will look into are:

• Has resistance to the currently used acaricides developed in two-spotted spider mites in

the most important strawberry and raspberry production sites in Norway?

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• Are there differences in the level of acaricide resistance and resistance development in

spider mites collected from plastic tunnels and field crops?

• How does the rotation of acaricides with different modes of action, reduced dosages and

spray coverage on the plants affect acaricide resistance development in the spider mites?

• Can the mite control strategy be changed in such a way that the risk for acaricide

resistance is reduced without loss of berry yield and quality?

Project period: 01-03 2016 – 31-12-2018.

Time for doing experiments: May-September each year.

Type of studies: Resistance tests (bioassays) in laboratory and/or efficacy experiments in

fields. Development of anti-resistance strategies for mite control in strawberry and raspberries

at farm and growing district level.

Read more about pesticide resistance:

Plantevernmiddelresistens hos skadedyr:

http://www.bioforsk.no/ikbViewer/Content/117065/TEMA_10-

1_PVMRes_v06_sendt%20Peder%20Lombn%C3%A6s%2026%20jan%202015.pdf

FAO Guidelines on Prevention and Management of Pesticide Resistance:

http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/Code/FAO_R

MG_Sept_12.pdf

EPPO standard on pest risk analysis PP1/213(3): http://pp1.eppo.int/getnorme.php?id=260

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30 Insecticide resistance monitoring and management

Supervisor: Nina Svae Johansen, NIBIO/NMBU. [email protected]

Background: Pesticide has become an increasing problem in Norwegian agriculture. Insect

pests of concern are the e.g. pollen beetles (Brassicogethes/ Meligethes spp.) and flea beetles

in oilseed crops, diamondback moth (Plutella xylostella) in cruciferous crops and two-spotted

spider mite (Tetranychus urticae) in soft fruits berry production. Some populations of these

pests have developed resistance towards one or more pesticides. In order to obtain less and a

more sustainable use of insecticides, and reduce the risk of resistance development, there is a

need to develop resistance management strategies for use at farm and district level, and to

include anti-resistance strategies in integrated pest management programs.

Fig. 1. Adult and egg of two-

spotted spider mite (Photo:

K. Westrum, NIBIO)

Fig. 2. Adult pollen

beetles on spring rape

flower buds. (Photo: E.

Fløistad, NIBIO)

Fig. 3 Larvae of diamondback

moth (Photo: E. Fløistad,

NIBIO).

Thesis: Different approaches are relevant, and a specific approach must be made based on the

student's field of interests. The master thesis will be a part of the projects Pesticide resistance:

Mutation, selection and dispersal (RESISTOPP) 2017-2021 or Pesticide resistance:

Preparedness and anti-resistance strategies (2017-2019).

Examples of topics are:

Monitoring the susceptibility in one of the mentioned pests towards insecticides used for

their control. The work will include collection of pests from field, resistance tests

(bioassays) in laboratory and/or efficacy experiments in fields.

Effect of the composition of the pollen beetle species-complex on resistance level: The work

will include identification of pollen beetles collected from oilseed rape fields with

morphological or molecular methods (beetles collected in 2016-2018 are available as well)

and resistance tests (historical and/ or new data).

Effect of resistance development on the fitness of two-spotted spider mites. The work will

include resistance selection in a strain of the two spotted spider mites, and experiments to

study of how resistance affects the biology of the mites. Laboratory experiments.

Development of anti-resistance strategies for pest control in one of the crops for use at farm

and district level: The work will include literature study, compilation of current knowledge

on resistance management and interviews with agricultural advisors and farmers, and can

be combined with one of the above-mentioned tasks.

You will get help with the handling of insecticides.

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Time for doing different tasks: Field collection of pests during May-August; resistance testing

with bioassays during May-December; fitness-resistance studies in laboratory and development

of and anti-resistance strategies during January-December.

Read more (in Norwegian):

Johansen NS, Asalf B, Eikemo H, Ficke A, Herrero M, Le VH, Netland J, Ringselle B, Schjøll

AF, Stensvand A, Strømeng GM (2017). Plantevernmiddelresistens hos skadegjørere i

norske jord- og hagebrukskulturer. NIBIO Rapport 3 (150) 2017. 60 pp.

https://brage.bibsys.no/xmlui/handle/11250/2481104

Pollen beetles: https://www.plantevernleksikonet.no/l/oppslag/73/

Diamondback moth: https://www.plantevernleksikonet.no/l/oppslag/93/

Two spotted spider mites: https://www.plantevernleksikonet.no/l/oppslag/19/

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31 Biological control of the new thrips Thrips setosus in protected

crops

Supervisor: Nina Svae Johansen, NIBIO/NMBU. [email protected]

Background: A new thrips (Thrips setosus) from Asia, has spread very rapidly in European

countries during the last 3 years. As this thrips can cause considerable damage in, it has been

of concern to the European Plant Protection Organization (EPPO). In 2018, T. setosus was

introduced to Norwegian greenhouses on imported plants. Currently, this thrips can only be

controlled by insecticides. This is a problem for growers who use biological control as their

main control strategy. Predatory mites, predatory bugs and nematodes are approved and used

for biological control against thrips in Norway, but we do not know if they are able to control

T. setosus.

Fig. 1. Adult Thrips setosus. Fig. 2. Thrips setosus on the underside

of a leaf and thrips damage on petals.

Thesis: Investigate if any of the approved natural enemies can be used for biological control of

the new thrips T. setosus. According to the student’s fields of interest, the work can include

predation/infection studies of one or two selected natural enemies in laboratory experiments or

in controlled climate chambers. It might be possible to do biological control experiments in a

commercial greenhouse if a grower are willing to act as a host (this might imply travelling to

the greenhouse).

The student should take contact early, because we need to establish a culture of T. setosus well

in advance before the experiments can start.

Read about Thrips setosus:

In Norwegian: https://www.plantevernleksikonet.no/l/oppslag/1882/

In English: https://gd.eppo.int/taxon/THRISE

Page 10: Master- and Bachelor Thesis Topics 2018-2019 Master

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32 Management of the alien invasive horse-chestnut leafminer

Cameraria ohridella

Supervisor: Nina Svae Johansen, NIBIO/NMBU. [email protected]

Background: The horse-chestnut leafminer (Cameraria ohridella) was first described in

Macedonia in 1984, it then spread rapidly accross Europe. The larvae, mining in the leaves, has

caused considerable damage on horse-chestnut threes since the 1980’es. The horse-chestnut

leaf miner was found for the first time in Drøbak in Norway in 2013, and has so far been found

at several locations around Oslofjorden, including in the park at NMBU, and at one location in

Vest-Agder. There are little experience on how this pest can be managed in Norway.

Fig. 1. Adult horse-chestnut leaf miner Fig. 2. Leaf mines made by larvae of horse-

chestnut leaf miner

Thesis: Different approaches are relevant, and a specific approach must be made based on the

student's field of interests.

Examples of topics are:

Investigate current distribution of the horse-chestnut leafminer in Norway.

At selected location(s):

o Phenology of the horse-chestnut leafminer

o Survey of natural enemies of the horse-chestnut leafminer at selected locations

o Investigate winter survival of the horse-chestnut leafminer at selected locations

Develop a guideline for prevention and management of horse-chestnut leafminer on trees

in urban areas.

Read more about the horse-chestnut leafminer:

In Norwegian: https://www.plantevernleksikonet.no/l/oppslag/1850/

In English: https://gd.eppo.int/taxon/LITHOD