measuring the fermentation broth of nadh photoelectric sensor
DESCRIPTION
Measuring the fermentation broth of NADH photoelectric sensor. 量測發酵液之 NADH 光電感測器. Presenter: Shih- Chieh Chao Adviser: Dr. Hung-Chi Yang Chairman: Dr. Hung-Chi Yang Date:05.08.2013. Outline. Background Introduction Purpose Methods & Materials Experiment Future works Reference. - PowerPoint PPT PresentationTRANSCRIPT
量測發酵液之NADH光電感測器
Measuring the fermentation broth of NADH photoelectric sensor
Presenter: Shih-Chieh ChaoAdviser: Dr. Hung-Chi Yang
Chairman: Dr. Hung-Chi YangDate:05.08.2013
1
OutlineBackgroundIntroductionPurposeMethods & MaterialsExperiment Future worksReference
2
BackgroundFluorescent luminous phenomenon is a light cooling. When
some kind of substance at room temperature by a certain wavelength of the incident light (usually ultraviolet or X-ray) radiation, absorb light energy into excited states and decay and issued by the outgoing light.
Human tissue containing many fluorescent substances, including collagen, NADH, FAD, rhodopsin, will be excited by UV fluorescence.
3
BackgroundHuman tissue in the cancer process, will produce a change
in the structure, such as: the cells become less dense connective tissue collagen tissue to reduce; metabolism of cancer cells than normal cells, so that the increased NADH; caused by increased blood flow absorbed rhodopsin rise. Therefore, the fluorescence spectrum of the cancer tissue and normal tissue will be different, and performance in the fluorescence spectral peak position and strength of the ebb and flow.
4
IntroductionNADH( 煙 醯 胺 腺 嘌 呤 二 核 苷 酸 ) NADH is a
widespread variety of animals, plants and humans in living cells, natural substances , and is a very important compound, was not discovered until 1905, usually with many enzymes in the body co-participate in the reaction, usually called "coenzyme“.
5
IntroductionCell growth and energy metabolism in the cells are required
to NADH, food into energy process requires NADH participate, and in the cell to produce energy in the process, NADH plays the important role of the portable electronic, in addition to NADH is also a very important the antioxidants can protect cells from damage of the harmful substances.
6
IntroductionNADH Excitation light of 340 ± 20nm.
7
Introduction
UV-A UV-B UV-C
Wavelength 400 ~ 315nm 315 ~ 280nm 280 ~ 200nm
Nociceptive weak medium strong
8
IntroductionThe most important function of the Fermenter, is to make
the microorganisms in the growth conditions it needs to reach the desired proliferation.
Therefore the design and production of the fermentation tank you need to have a lot of considerations.
9
IntroductionNeed to maintain long-aseptic operation.Microbial metabolism of aeration and agitation, but can not
give microbial mechanical shock stirring intensity.Need to configure the device temperature system Imperial.Need to be able to control the pH.Need to be able to sample.Must be appropriate size.Internally to maintain a smooth inner surface, not have
welded junction.By fermentation of a variety of different, you need to install
the necessary equipment incidental.
10
Introduction
11
PurposeThis study focused on :
The sensor can be fully inserted fermenterContinuous and real-time performance measurement
12
Detector
Broth+
fungusLED
MCU Control
PC
Purpose
13
340 nm
450 nm
Purpose
30F4011
UART
PWM
TIMER
A/D
LED*4
DETPC
RS232
14
Methods & MaterialsSolidwork
15
Methods & MaterialsType 304—the most common grade; the classic 18/8 stainless steel.
Outside of the US it is commonly known as "A2 stainless steel”.Type 316—the second most common grade (after 304); for food and
surgical stainless steel uses; alloy addition of molybdenum prevents specific forms of corrosion. It is also known as marine grade stainless steel due to its increased resistance to chloride corrosion compared to type 304. 316 is often used for building nuclear reprocessing plants.
16
Methods & Materials
17
Methods & Materials
18
LED
Detector
Methods & Materials
19
Methods & Materials
20
10mm
44mm
20mm
44mm
40mm
10mm
20mm12mm
8mm
Methods & Materials
40mm
21
Methods & Materials
22
Methods & Materials
23
30mm
14mm
26mm
18mm14m
m26mm30mm
Methods & Materials
26mm24
Methods & MaterialsExcitation light : NSHU551B
25
Methods & MaterialsKupo : UV Transmission
300 400 500 600 nm
stut
0102030405060708090
100%T
26
Methods & MaterialsNADH emit light of 450±20nm , so the selection of the
received light 320-730nm.Hamamatsu : S1133
spectral response range 320~730nm
27
Methods & MaterialsKupo : C11715
300 400 500 600 nm
stut
0102030405060708090
100%T
28
Methods & MaterialsKupo : UV Blocking
300 400 500 600 nm
stut
0102030405060708090
100%T
29
300 400 500 600 nm
stut
0102030405060708090
100%T
Methods & MaterialsUV Blocking+C11715
300 400 500 600 nm
stut
0102030405060708090
100%T
30
Experiment
31
Experiment
32
Experiment
33
Experiment
34
ExperimentFluorescein, 90+% L13251Formula: : C20H12O5 Molecular weight :332.32Orange powder
35
Experiment
5mL AOH(47.5%) 5mL fluoresein(1mM)
0.5mM
5ml AOH(47.5%) 5mL fluoresein(0.5mM)
0.25mM
5ml AOH(47.5%) 5mL fluoresein(0.25mM)
0.125mM
5ml AOH(47.5%) 5mL fluoresein(0.125mM)
0.0625mM
Deployment method, beginning first with a 1-to-1 water diluted to 47.5% alcohol with 95% alcohol(AOH)
36
Experiment
37
Experiment
38
ExperimentSigma N8129 :
Reduced coenzyme Yellowish white powderStored at -20 ℃Excitation light = 340 nm 。Emit light = 460 nm 。
39
Experiment Fluorescence Spectrometer F-7000
40
Future worksCircuit design30F4011Labview
41
Reference[1] Y-C Liu, F-S Wang, W-C Lee, “ On-line monitoring and controlling system for fermentation processes. “
Biochemical Engineering Journal 7 (2001) 17–25.[2] Monici M. (2005). "Cell and tissue autofluorescence research and diagnostic
applications".BiotechnolAnnu.Rev.11:227–56.doi:10.1016/S1387-2656(05)11007-2. PMID 16216779.[3] Georgakoudi I, Jacobson BC, Müller MG, Sheets EE, Badizadegan K, Carr-Locke DL, Crum CP, Boone CW, Dasari
RR, Van Dam J, Feld MS (2002-02-01). "NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes". Cancer Res. 62 (3): 682–687. PMID 11830520
[4] Windholz, Martha (1983). The Merck Index: an encyclopedia of chemicals, drugs, and biologicals (10th ed.). Rahway NJ, US: Merck. p. 909. ISBN 911910271 Check |isbn= value (help).
[5] Bakker BM, Overkamp KM, van Maris AJ, et al. (2001). "Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae". FEMS Microbiol. Rev. 25 (1): 15–37.
[6] Nicholls DG; Ferguson SJ (2002). Bioenergetics 3 (1st ed.). Academic Press. ISBN 0-12-518121-3.[7] Sistare FD, Haynes RC (15 October 1985). "The interaction between the cytosolic pyridine nucleotide redox potential
and gluconeogenesis from lactate/pyruvate in isolated rat hepatocytes. Implications for investigations of hormone action". J. Biol. Chem. 260 (23): 12748–53. PMID 4044607.
[8] Freitag A, Bock E (1990). "Energy conservation in Nitrobacter". FEMS MicrobiologyLetters 66 (1–3): 157–62. doi:10.1111/j.1574-6968.1990.tb03989.x.
[9] Starkenburg SR, Chain PS, Sayavedra-Soto LA, et al. (2006). "Genome Sequence of the Chemolithoautotrophic Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Nb-255". Appl. Environ. Microbiol. 72 (3): 2050–63. doi:10.1128/AEM.72.3.2050-2063.2006. PMC 1393235. PMID 16517654.
[10] Dawson, R. Ben (1985). Data for biochemical research (3rd ed.). Oxford: Clarendon Press. p. 122. ISBN 0-19-855358-7.
[11] 曾百由 編著 “數位訊號控制器原理與應用”,洪有圖書開發股份有限公司,九十三年十一月[12] 庭田企業有限公司,網址: http://www.dichroic.com.tw/cn/index.htm 。[13] 日亞化學株式會社,網址: http://www.led-shop.com.tw/index.htm 。42
The End
43