molecular mechanisms of yield & abiotic stress tolerance in rice joint laboratory for plant cell...

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Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE, Chinese Academy of Science) Professor JIANG Liwen (The Chinese University of Hong Kong) 中中中—中中中中中中中中中中中中中中中中中中 Review Meeting of the CAS-Croucher Funding Scheme for Joint Laboratories Kunming (18 th October 2012)

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Page 1: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice

Joint Laboratory for Plant Cell Biology & Rice Genetics 

Professor LIN Hong-Xuan (SIPPE, Chinese Academy of Science)

Professor JIANG Liwen (The Chinese University of Hong Kong) 

 

中科院—裘槎基金联合实验室资助计划项目总结会

Review Meeting of the CAS-Croucher Funding Scheme for Joint Laboratories

Kunming (18th October 2012)

Page 2: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

• Rice Genetics

• Rice breeding

• Rice Molecular biology

Prof. Lin (Rice Genetics)

Page 3: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Prof. Lin (Rice Genetics)

Page 4: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

• Plant Cell Biology

• Protein trafficking and localization

• Organelle biogenesis and function

Prof. Jiang (Cell Biology)

Page 5: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Prof. Jiang (Plant Cell Biology)

Page 6: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

• Genetics vs. Cell Biology

• Excellent track records in respective research areas

• Have on-going collaborative research

• Complement and syngenism 

The two PIs

Page 7: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Our goals and research plan• To understand the mechanisms of yield traits and stress tolerance in rice

• Potential application

• Important biological questions on rice biology and biotechnology

• Training of students and researchers

Page 8: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Yield traits of Rice

Molecular mechanisms of yield traits

Molecular breeding for high-yield crops

Molecular breeding for high-yield crops

Functional analysis of yield trait genes/QTLsFunctional analysis of yield trait genes/QTLs

Cell biological analysis of yield trait genes/QTLs

Cell biological analysis of yield trait genes/QTLs

Mapping and cloning of genes/QTLs related to yield traits

Mapping and cloning of genes/QTLs related to yield traits

NIL(GW2)                 S-7                     S-65                    S-85

FAZ1 NIL(GW2) FAZ1 NIL(GL3.1)

Research Plan

Page 9: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

 Stress tolerance traits of Rice

Molecular mechanisms of stress tolerance

Molecular breeding for crops with improved tolerance

Molecular breeding for crops with improved tolerance

Functional analysis of stress tolerance genes/QTLs

Functional analysis of stress tolerance genes/QTLs

Cell biological analysis of stress tolerance genes/QTLs

Cell biological analysis of stress tolerance genes/QTLs

Mapping and cloning of genes/QTLs related to stress tolerance

Mapping and cloning of genes/QTLs related to stress tolerance

Research Plan

Page 10: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Progress of on-going collaboration

Page 11: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Mapping based cloning and functional analysis of grain length QTL GL3.1

GL3.1

Cloning of GL3.1

Overexpress GL3.1-WY3 increase the grain length

GL3.1 is predicted to encode a Ser/Thr

phosphatase with kelch domain

Page 12: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

GL3.1 increase the grain length, weight and yield

The plot grain yield was significantly increased in NIL (+11.1%)

Page 13: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Subcellular location of GL3.1

GL3.1 in both parents was detected throughout the entire cell

Page 14: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

Subcellular location of Cyclin-T1;3

When GL3.1 was co-expressed with Cyclin-T1;3, increased accumulation of GL3.1 was observed in the nuclei

Page 15: Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE,

• The Croucher Foundation• The CAS-Croucher Funding Scheme for Joint

Laboratories• CAS/SIPPE and CUHK