nk macrophages. 本章大綱 1. hematopoiesis ( 造血 ) 2. cells of the immune system 3. organs of...
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NKMacrophages
本章大綱
1. Hematopoiesis (造血 )
2. Cells of the Immune System
3. Organs of the Immune System
Hematopoiesis
Locations of hematopoiesis
Hematopoietic stem cell (HSC)
Embryonic stage:Yolk sac fetal liver spleen (3rd to 7th month) bone marrow
Self-renewdifferentiate
(embryonic stem cells, ES cells)
Clinical Use of Hematopoietic Stem Cells 1. Providing a functional immune system to individuals with a genetically immunodeficiency.
2. Replacing a defective hematopoitic system with a functional one (i.e. anemia).
3. Restoring the hematopoitic system of cancer patients after treatment with high doses of chemo- therapeutic agents or radiation.
Autologous : The recipient is also the donor.
Syngeneic : The donor is the identical twin of the recipient.
Allogeneic : The donor and the recipient are not genetically identical within the same species.
Xenogeneic : The donor and the recipient are from different species.
Bone-Marrow Transplantation
Cells for transplantation
Bone-marrow cells
Peripheral blood cells
Umbilical-cord blood cells (Cord Blood)
Stem-cell transplantation
Cells of the Immune System
Normal Adult Blood-Cell Count __________________________________
Cell type Cells/mm3 % ___________________________________________________________________________________________________________________________
Red blood cells 5.0 x 106
Platelets 2.5 x 105
Leukocytes 7.3 x 103
Neutrophil 50 – 70 Lymphocyte 20 – 40 Monocyte 1 – 6 Eosinophil 1 – 3 Basophil < 1 ____________________________________________
- Lymphoid Cells
B lymphocytes, T lymphocytes, Null Cells (natural killer, NK cells)
-Mononuclear Phagocytes Monocytes, Macrophages, dendritic cell
- Polymorphonuclear Cells (PMN) or granulocytic cells or granulocytes
Neutrophils, Eosinophils, Basophils, Mast cells, Dendritic cells
Lymphoid Cells
- 20% - 40% of the body’s white blood cells
- 99% of the cells in the lymph
- ~ 1010 - 1012 in the human body
- Circulate in the blood and lymph
- Migrate into the tissue spaces and lymphoid organs
T Lymphocytes: Thymus
B Lymphocytes: Bursa of Fabricius (黏液囊 ) or Bone Marrow
T Lymphocyte Markers
T cell receptor (TCR)CD4/CD8CD28--------B7CD45--------signal transduction
B Lymphocyte Markers
Immunoglobulin (Ig) B220 (CD45R) MHC Class II CR1 (CD35) & CR2 (CD21) FcRII (CD32) B7-1 (CD80) & B7-2 (CD86) CD40
Fate of antigen-activated small B lymphocytes
Short half life
1-2 week
Null Cells- Most are large, granular lymphocytes called natural killer (NK) cells.
- Against tumor cells and cells infected with some virus.
- Constitute 5 - 10% of the lymphocytes in human peripheral blood.
- NK T cells
Mononuclear Phagocytes
- Monocytes circulating in the blood
- Macrophages in the tissues
Macrophages are 5- to 10-fold larger than monocytes and contain more organelles, especially lysosomes
Macrophages
-Alveolar macrophages in the lung
-Histiocytes in connective tissues
-Kupffer cells in the liver
-Mesangial cells in the kidney
-Microglial cells in the brain
- Osteoclasts in bone
Functions of Macrophages
- Phagocytosis
- Antimicrobial and cytotoxic activities
- Antigen processing and presentation
- Secretion of factors
Granulocytic Cells(granulocytes)
- Neutrophils:phogocytosis
- Eosinophils: against parasite
- Basophils: nonphagocytosis; allergic
Two lobedEosin red
One lobedMethylene blue
Dendritic Cells
- Arises from the myeloid and lymphoid lineages.
-Presenting antigen to TH.
-MHC class II and B7 co-stimulatory
Skin, mucous organs
MHC class IIB7
Organs of the Immune System
Primary (central) lymphoid organs- Provide appropriate microenvironments for the development and maturation of lymphocytes.
-thymus, bone marrow
Secondary (peripheral) lymphoid organs- Trap antigen from tissues or vascular spaces and are sites where mature lymphocytes can interact effectively with that antigen.
- lymph nodes, spleen, mucosal-associated lymphoid tissues (MALT), e.g., gut-associated lymphoid tissue (GALT).
Tertiary lymphoid tissue - Cutaneous-associated lymphoid tissues
Lymphatic System (淋巴系統 )
- Once mature lymphocytes have been generated in the primary lymphoid organs, they circulate in the blood and lymphatic system, a network of vessels that collect fluid that has escaped into the tissues from capillaries of the circulatory system and ultimately return it to the blood.
Lymphatic Vessels
The Human Lymphoid Systemsecondary
primary
鎖骨下靜脈胸導管
The thymus
stromal cells:
m
aturation
lobule
+,-, selection
Bone Marrow
• Human, mice: bone marrow
• Cattle, sheep: fetal spleen, ileal peyer’s patch
• Rabbit: gut associated tissue, appendix
• Birds: bursa of fabricius
A Lymph Node (1)
B, dendritic, Macrophage
T, dendritic
Plasma cells
A Lymph Node (2)Ag
Primary follicle
Ag
Secondary follicleGerminal centers
Lymphocytes migrate from blood
The Spleen
Blood borne antigen
The Spleen (1)
(血管神經出入口 )
Ag
The Spleen (2)
MΦ, RBC
Lymphocytes, MΦ
T cells
Mucosal-Associated lymphoid tissue (MALT)
TonsilsAppendixPeyer’s patches
- contain a large population of Ab-producing plasma cells
Tonsils
(上顎 ) (舌 )
(咽 )
Peyer’s Patch
contain intraepitheliallymphocytes IELs
B cells, plasma cell,activated TH cells & m
Structure of M Cells
Basolaternal plasma membrane
Production of IgA Ab in the Gut
Use M cells to infection
• Polio virus
• Salmonella species
• Vibrio cholerae
Cutaneous-associated Lymphoid Tissue
Comparative Immunity
- Invertebrates and plants do not have adaptive immunity.
- antibacterial and antifungal peptides in insects
- antibiotic molecules in plants; phytoalexins
(八目鰻 )
本章大綱
1. Hematopoiesis (造血 )
2. Cells of the Immune System
3. Organs of the Immune System